In order to provide consumers with more safe and edible fungus products and to create a faithful social environment of food quality, the edible fungus products quality safety events, as well as their evoked factors an...In order to provide consumers with more safe and edible fungus products and to create a faithful social environment of food quality, the edible fungus products quality safety events, as well as their evoked factors and effects on edible fungus industry were discussed in depth in this paper. The performance of edible fungus products quality management is a comprehensive reflection of technical effect, control effect and implementation effect. Among them, the implementation effect is the most important, which refers to the actual implementation effect of quality and safety rules by practitioners of edible fungus industry. Thus, the initiative implementation effect in quality management will be improved, and a quality assurance system in which practitioners are volunteered to participate and implement initiatively the quality standards and regulations so as to provide safe and high-quality products will be established, thereby achieving the best actual implementation effect in quality management.展开更多
The increasing demand for natural food antioxidants has hastened research to extract the biologically active substances from a variety of raw materials. The consumption of anthocyanin-rich food promotes health by con...The increasing demand for natural food antioxidants has hastened research to extract the biologically active substances from a variety of raw materials. The consumption of anthocyanin-rich food promotes health by contributing to cardio protection, lowering high blood pressure and by helping to protect against cancer. In this study, centrifugal partition chromatography （CPC） was studied for anthocyanin separation from Hibiscus sabdariffa L. （HS）. A simple elution and two displacement protocols were implemented. The displacement modes were strong ion-exchange （SIX） and pH-zone refining （pHZR） method. Among the three protocols, SIX CPC using trioctylmethylammonium chloride （Aliquat 336 ？） as a strong exchanger was the most successful in purifying four anthocyanins from an ethanolic extract of HS, identified as delphinidin-3-O-sambubioside （DS）, cyanidin-3-O-sambubioside （CS）, delphinidin-3-O-glucoside （DG） and cyanidin-3-O-glucoside （CG）. The purification process was carried out on a gram scale using the ternary biphasic system ethyl acetate：n-butanol：water （mobile organic phase： 4.0/4.6/1.4; aqueous stationary phase： 0.5/0.5/9.0） in the ascending mode. Aliquat 336？ was used as the anion extractant in the aqueous stationary phase and sodium iodide （NaI） as the cation displacer in the organic mobile phase. From 1 g of crude HS extract, 80 mg of DS, 40 mg of CS, 15 mg of DG, and 4 mg of CG were obtained after 75 minutes. High performance liquid chromatography （HPLC-UV and LC-MS） analysis revealed the identity and the purity of the isolated compounds： 88% for DS, 92% for CS, 93% for DG and 70% for CG. This new methodology for the isolation of anthocyanin mixtures successfully increased the purity and ef？ciency especially for DG and CG, while maintaining excellent recovery rate and being relatively low cost. The availability of high purity anthocyanin mixtures will facilitate anthocyanin testing studies and will widen the use of anthocyanins in the food, supplements and pharmaceutical industries.展开更多
基金Sponsored by Coal-based Key Scientific and Technological Project of Shanxi Province(FT2014-03)Doctoral Foundation of Shanxi Academy of Agricultural Sciences(YBSJJ1616)
文摘In order to provide consumers with more safe and edible fungus products and to create a faithful social environment of food quality, the edible fungus products quality safety events, as well as their evoked factors and effects on edible fungus industry were discussed in depth in this paper. The performance of edible fungus products quality management is a comprehensive reflection of technical effect, control effect and implementation effect. Among them, the implementation effect is the most important, which refers to the actual implementation effect of quality and safety rules by practitioners of edible fungus industry. Thus, the initiative implementation effect in quality management will be improved, and a quality assurance system in which practitioners are volunteered to participate and implement initiatively the quality standards and regulations so as to provide safe and high-quality products will be established, thereby achieving the best actual implementation effect in quality management.
文摘The increasing demand for natural food antioxidants has hastened research to extract the biologically active substances from a variety of raw materials. The consumption of anthocyanin-rich food promotes health by contributing to cardio protection, lowering high blood pressure and by helping to protect against cancer. In this study, centrifugal partition chromatography （CPC） was studied for anthocyanin separation from Hibiscus sabdariffa L. （HS）. A simple elution and two displacement protocols were implemented. The displacement modes were strong ion-exchange （SIX） and pH-zone refining （pHZR） method. Among the three protocols, SIX CPC using trioctylmethylammonium chloride （Aliquat 336 ？） as a strong exchanger was the most successful in purifying four anthocyanins from an ethanolic extract of HS, identified as delphinidin-3-O-sambubioside （DS）, cyanidin-3-O-sambubioside （CS）, delphinidin-3-O-glucoside （DG） and cyanidin-3-O-glucoside （CG）. The purification process was carried out on a gram scale using the ternary biphasic system ethyl acetate：n-butanol：water （mobile organic phase： 4.0/4.6/1.4; aqueous stationary phase： 0.5/0.5/9.0） in the ascending mode. Aliquat 336？ was used as the anion extractant in the aqueous stationary phase and sodium iodide （NaI） as the cation displacer in the organic mobile phase. From 1 g of crude HS extract, 80 mg of DS, 40 mg of CS, 15 mg of DG, and 4 mg of CG were obtained after 75 minutes. High performance liquid chromatography （HPLC-UV and LC-MS） analysis revealed the identity and the purity of the isolated compounds： 88% for DS, 92% for CS, 93% for DG and 70% for CG. This new methodology for the isolation of anthocyanin mixtures successfully increased the purity and ef？ciency especially for DG and CG, while maintaining excellent recovery rate and being relatively low cost. The availability of high purity anthocyanin mixtures will facilitate anthocyanin testing studies and will widen the use of anthocyanins in the food, supplements and pharmaceutical industries.
