[ Objective] The aim of this study was to isolate the endophyte of three solanaceae fruits and vegetables such as tomato, pepper and eggplant, to screen and identify the bacterial wilt antagonistic bacteria. [ Methodl...[ Objective] The aim of this study was to isolate the endophyte of three solanaceae fruits and vegetables such as tomato, pepper and eggplant, to screen and identify the bacterial wilt antagonistic bacteria. [ Methodl According to the lapping liquid culture method, the endophyte of three plants was isolated by the selective medium and purified by the plate streaking method, so the purified enclophyte was screened by the hyphal pieces confront culture method. Furthermore, the screened antagonistic and endophyteic bacteria was identified and classified through culture characteristics of isolates and morphological features of thallus, Gram stain as well as physiological and biochemical reactions. [ Result] Fifty-three endophytic bacteria, fifty-three endophytic fungi and forty-four endophytic actinomycetes were separated from the endophyte of three plants. The screened fourteen endophytic bacteria with strong antagonistic effect on the bacterial wilt were classified to Bacillus, Escherichia, Klebsiella, Agromonas, Erwinia and Curto Bacterium respectively. Especially, Bacillus was the dominant species, which had the strongest antagonistic effect on the bacterial wilt. [ Conclusion] This study provides an effective way for biological control of the bacterial wilt in solanaceae.展开更多
文摘[ Objective] The aim of this study was to isolate the endophyte of three solanaceae fruits and vegetables such as tomato, pepper and eggplant, to screen and identify the bacterial wilt antagonistic bacteria. [ Methodl According to the lapping liquid culture method, the endophyte of three plants was isolated by the selective medium and purified by the plate streaking method, so the purified enclophyte was screened by the hyphal pieces confront culture method. Furthermore, the screened antagonistic and endophyteic bacteria was identified and classified through culture characteristics of isolates and morphological features of thallus, Gram stain as well as physiological and biochemical reactions. [ Result] Fifty-three endophytic bacteria, fifty-three endophytic fungi and forty-four endophytic actinomycetes were separated from the endophyte of three plants. The screened fourteen endophytic bacteria with strong antagonistic effect on the bacterial wilt were classified to Bacillus, Escherichia, Klebsiella, Agromonas, Erwinia and Curto Bacterium respectively. Especially, Bacillus was the dominant species, which had the strongest antagonistic effect on the bacterial wilt. [ Conclusion] This study provides an effective way for biological control of the bacterial wilt in solanaceae.
