[Objective] The aim was to explore the species diversity of flower-visiting Syrphidae in the farmland of Hanzhong area.[Method] Based on the sample collection and the sorting and identification of collected specimens ...[Objective] The aim was to explore the species diversity of flower-visiting Syrphidae in the farmland of Hanzhong area.[Method] Based on the sample collection and the sorting and identification of collected specimens and literature,the species and their seasonal changes of flower-visiting Syrphidae in the agroecosystem of Hanzhong area were systematically analyzed.[Result] 5 015 specimens of Syrphidae were cheaked in this study,and 141 species of 53 genera of 16 families in three subfamilies of flower-visiting species of Syrphidae were identified in farmland of Hanzhong area,in which 65 species were Syrphinae;72 species were Milesiinae;while four species were Microdontinae.The species of Syrphidae in this region were active in April,May,June and July.There were two dominant species in this region,including Episyrphus balteatus and Eristalis tenax.[Conclusion] This study had provided theoretical basis for the studies on the flower-visiting habit and fecundation of insects of Syrphidae in this area.展开更多
[ Objective] The aim of this study was to isolate the endophyte of three solanaceae fruits and vegetables such as tomato, pepper and eggplant, to screen and identify the bacterial wilt antagonistic bacteria. [ Methodl...[ Objective] The aim of this study was to isolate the endophyte of three solanaceae fruits and vegetables such as tomato, pepper and eggplant, to screen and identify the bacterial wilt antagonistic bacteria. [ Methodl According to the lapping liquid culture method, the endophyte of three plants was isolated by the selective medium and purified by the plate streaking method, so the purified enclophyte was screened by the hyphal pieces confront culture method. Furthermore, the screened antagonistic and endophyteic bacteria was identified and classified through culture characteristics of isolates and morphological features of thallus, Gram stain as well as physiological and biochemical reactions. [ Result] Fifty-three endophytic bacteria, fifty-three endophytic fungi and forty-four endophytic actinomycetes were separated from the endophyte of three plants. The screened fourteen endophytic bacteria with strong antagonistic effect on the bacterial wilt were classified to Bacillus, Escherichia, Klebsiella, Agromonas, Erwinia and Curto Bacterium respectively. Especially, Bacillus was the dominant species, which had the strongest antagonistic effect on the bacterial wilt. [ Conclusion] This study provides an effective way for biological control of the bacterial wilt in solanaceae.展开更多
[Objective] This study aimed to establish Balb/c mice model for food allergy caused by Chinese lobsters through using intraperitoneal injection for sensitization and explore methods for in vitro identification and eva...[Objective] This study aimed to establish Balb/c mice model for food allergy caused by Chinese lobsters through using intraperitoneal injection for sensitization and explore methods for in vitro identification and evaluation of food allergy caused by Chinese lobsters. [Method] The 40 male Bal/c mice were divided into ovalbumin(OVA) positive control group, Coca's solution negative control group, blank control group and model group. Balb/c mice model was established by intraperitoneally injection of immunized Balb/c mice with OVA or Chinese lobster crude protein with aluminum hydroxide adjuvant. IgE and histamine levels in serum after the second challenge were determined by ELISA method, and the specific IgE antibody titer was determined by passive cutaneous anaphylaxis test(PCA); additionally, spleen index and histological changes in the small intestine, as well as food allergy symptoms after challenge were also calculated or observed. [Results] After the last challenge, IgE content was(236.75 ±73.39) μg/L in the Chinese lobster crude protein group, revealing no difference with that in the OVA group, but significantly higher than that in either the Coca's solution group or the blank control group(P 0.01);histamine content in serum in the Chinese lobster crude protein group was(406.55±232.79), significantly higher than that in the blank control group(P0.01). In the passive cutaneous anaphylaxis test, IgE antibody titer reached 1/16 after the last challenge in the Chinese lobster crude protein group. Spleen index in both Chinese lobster crude protein group and OVA group was significantly greater than that in either the Coca's solution group or the blank control group(P0.01). What's more, infiltration of inflammatory cells like lymphocytes and eosnophils at the lamina propria of intestinal mucosa was also observed both Chinese lobster crude protein group and OVA group. [Conclusion] This study established Balb/c mice model for food allergy caused by Chinese lobsters; serum IgE and ELISA assay and specific IgE antibody titer in PCA test can be used for the in vitro identification and evaluation of food allergies caused by Chinese lobsters.展开更多
Food allergy is a common and increasing problem worldwide. The newly-found knowledge might provide novel experimental strategies, especially for laboratory diagnosis. Approximately 20% of the population alters their d...Food allergy is a common and increasing problem worldwide. The newly-found knowledge might provide novel experimental strategies, especially for laboratory diagnosis. Approximately 20% of the population alters their diet for a perceived adverse reaction to food, but the application of double-blind placebo-controlled oral food challenge, the "gold standard" for diagnosis of food allergy, shows that questionnaire-based studies overestimate the prevalence of food allergies. The clinical disorders determined by adverse reactions to food can be dassified on the basis of immunologic or nonimmunologic mechanisms and the organ system or systems affected. Diagnosis of food allergy is based on clinical history, skin prick tests, and laboratory tests to detect serum-food specific IgE, elimination diets and challenges. The primary therapy for food allergy is to avoid the responsible food. Antihistamines might partially relieve oral allergy syndrome and IgE-mediated skin symptoms, but they do not block systemic reactions. Systemic corticosteroids are generally effective in treating chronic IgE-mediated disorders. Epinephrine is the mainstay of treatment for anaphylaxis. Experimental therapies for IgE-mediated food allergy have been evaluated, such as humanized IgG anti-IgE antibodies and allergen specific immunotherapy.展开更多
AIM: To prepare a kind of magnetic iron-dextran nanopartides that was coated with anti-E.coli O157:H7 IgG, analyze its application conditions, and try to use it to isolate E.coli O157:H7 from foods. METHODS: Magnetic ...AIM: To prepare a kind of magnetic iron-dextran nanopartides that was coated with anti-E.coli O157:H7 IgG, analyze its application conditions, and try to use it to isolate E.coli O157:H7 from foods. METHODS: Magnetic iron-dextran nanopartides were prepared by the reaction of a mixture of ferric and ferrous ions with dextran polymers under alkaline conditions. The particles were coated with antiserum against E.coli O157: H7 by the periodate oxidation-borohydride reduction procedure. The oxidation time, amount of antibody coating the particles, amount of nanoparticles, incubation time and isolation time were varied to determine their effects on recovery of the organisms. Finally, the optimum conditions for isolating E.coli O157:H7 from food samples were established. RESULTS: E.coli O157:H7 can be isolated from samples within 15 min with the sensitivity of 101 CFU/mL or even less. In the presence of 108 CFU/mL of other organisms, the sensitivity is 101-102 CFU/mL. Nonspecific binding of other bacteria to the particles was not observed. Two and a half hours of enrichment is enough for the particles to detect the target from the food samples inoculated with 1 CFU/g. CONCLUSION: Isolation of target bacteria by immuno magnetic nanoparticles is an efficient method with high sensitivity and specificity. The technique is so simple that it can be operated in lab and field even by untrained personnel.展开更多
A device to activate lignite and sludge by electro-hydraulic impulse is presented. It comprises an impulse current generator constituted by a high voltage transformer, a high voltage silicon rectifier, a current-limit...A device to activate lignite and sludge by electro-hydraulic impulse is presented. It comprises an impulse current generator constituted by a high voltage transformer, a high voltage silicon rectifier, a current-limiting resistor, a capacitor bank and an air-break switch, and a lignite and sludge appliance made up of two plane electrodes and a working chamber. The installation activates the lignite or sludge delivered to the working chamber by the impulse current that is from the generator to puncture the main gap in the working chamber, raise the temperature therein up to (20 000 to 40 000) K and the energy density to as high as 109 Jm-3, and form a plasma piston. The alternative development and attenuation of plasma expansion makes the lignite in a number of physical and chemical processes resulting in diverse active radicals and ions, and also breaks it into grains mostly smaller than 250 mm. It is founded by experimental study that the technology can raise the content of nitro-nitrogen by 1.4 to 1.5 times and that of dissoluble organic substances by 5 to 10 times which is probably attributed to the decomposition of the ample germs in the lignite. The calculated power requirement to activate lignite is about (50 to 60) kWht-1. In comparison with muck applied to cucumber cultivation, the activated lignite demonstrates its features as an effective and economical green fertilizer by the same yield with half amount. These findings imply a promising access to green fertilizer.展开更多
AIM: To explore the effect of Sinai san decoction on the development of non-alcoholic steatohepatitis induced by CCL4 combined with a fat-rich diet in rats.METHODS: Twenty-seven Sprague-Dawley rats were divided into t...AIM: To explore the effect of Sinai san decoction on the development of non-alcoholic steatohepatitis induced by CCL4 combined with a fat-rich diet in rats.METHODS: Twenty-seven Sprague-Dawley rats were divided into three groups randomly: control group (n = 9),model group (n = 9) and treatment group (n = 9). The rats of model group and treatment group were given small dosage of CCL4 combined with a fat-rich diet, andthose of control group were given normal diet. After four weeks of fat-rich diet feeding, the rats of treatment group were given Sinai san decoction. The serum levels of aminotransferase and lipid were measured, and the pathology of livers was observed by HE staining after the rats were sacrificed at eight weeks.RESULTS: The rats' livers presented the pathology of steatosis and inflammation with higher serum levels of ALT and AST in the model group. In the treatment group the serum ALT and AST levels decreased significantly and were close to the control group. The hepatic inflammation scores also decreased markedly, but were still higher than those of control group. And the degree of hepatocyte steatosis was similar to that of model group.CONCLUSION: Sinai san decoction may ameliorate the hepatic inflammation of rats with steatohepatitis induced by small dosage of CCL4 combined with a fat-rich diet,but does not prevent the development of hepatocyte steatosis.展开更多
Insects are major pests of stored grains. In sub Sahara Africa, stored grain insect pests are mainly Coleoptera or Lepidoptera. In the Logone Valley, area covering the Far North Region of Cameroon and South-West of Ch...Insects are major pests of stored grains. In sub Sahara Africa, stored grain insect pests are mainly Coleoptera or Lepidoptera. In the Logone Valley, area covering the Far North Region of Cameroon and South-West of Chad, to our knowledge, the diversity of insect pests noxious to stored products is not established. These beetles and weevils (Insecta:Coleoptera) during their development depreciate food in storage, were able to destroy a whole stock in a very short period of time if no protection of stored food is made. To achieve the diversity of insect pests, inspections of granaries were done in 209 villages of Logone Valley where insect pests were collected and identified. Secondly, the stored grains were sampled in peasant's granaries of Logone Valley and reared in incubators in the Unit of Entomological Research of the University of Ngaound6r6. After their emergence, insects were removed, collected and identified. Their noxious efficiency on stored grains is done by the calculation of the density of each insect pests per gram of grains. This study indicates that 12 species of harmful insects attack cereals and leguminous grains in the Logone Valley during storage. The Bruchids were the main insect pests of legumes. Callosobruchus maculatus was the main pest of legume and attacking mainly cowpeas (34.92 bruchids/g of seed) and peanuts (20.75 bruchids/g of seed). Seven harmful species destroy the cereals. Sitophilus zeamais is the main pest of maize (15.86 weevils/g of maize) and S. oryzae, for sorghum (36.59 weevils/g of maize). Tribolium castaneum were the main secondary pest. T. castaneum infests both cereals and leguminous. This work summarizes the importance and the diversification of stored grain pests in Logone Valley. Knowing the pest diversity is an important step in the strategy to develop a friendly and ecological control method of these harmful insects.展开更多
This paper is divided into three subjects: Japanese Genghis Khan, Chinese tofu and Korean bibimbap (mixed rice with vegetables). It will be a cross-cultural and comparative research on historic foods among three co...This paper is divided into three subjects: Japanese Genghis Khan, Chinese tofu and Korean bibimbap (mixed rice with vegetables). It will be a cross-cultural and comparative research on historic foods among three countries (Japan, China, and Korea), in relation to national culinary identity.展开更多
Stable carbon and nitrogen isotopes were used to investigate nutrient pathways and trophic relationships from the rice-crab system in Panjin, Liaoning Province, China. Values of 313C ranged from -27.38%±0.44%o to...Stable carbon and nitrogen isotopes were used to investigate nutrient pathways and trophic relationships from the rice-crab system in Panjin, Liaoning Province, China. Values of 313C ranged from -27.38%±0.44%o to -18.34%±0.26%o and δ^15N ranged from 1.10%o4-0.88%o to 9.33%±0.57%. Pseudorasbora parva (Stone moroko) had the highest δ^13C and 8tSN values. The lowest δ^13C values were obtained for the macrophytes and the lowest δ^15N value was found in sediments. Stable carbon and nitrogen isotopes were used to determine the contribution of different food items to the diets of crabs. The δ^13C results indicated that the Pseudorasbora parva made the greatest contribution to the diet of Eriocheir sinensis (Chinese mitten crab), while the δ^15N results indicated that most food items contributed more than 10% to the diet of the crab. There were three trophic levels identified in the system (Levels 0-2). The crab Eriocheir sinensis, fish Pseudorasbora parva and Misgurnus anguillicaudatus (Oriental weatherfish), and the oligochaete Limnodrilus hoffmeisteri (Limnodrilus worm), were at the second level, zooplankton were at the first level and suspended particulate matter and macrophytes were at trophic position 0.展开更多
基金Supported by National Natural Science Foundation of China(31071966)Natural Science Foundation of Shaanxi Province(2009JM3007)Innovative Research Projects for Students in Shaanxi University of Technology~~
文摘[Objective] The aim was to explore the species diversity of flower-visiting Syrphidae in the farmland of Hanzhong area.[Method] Based on the sample collection and the sorting and identification of collected specimens and literature,the species and their seasonal changes of flower-visiting Syrphidae in the agroecosystem of Hanzhong area were systematically analyzed.[Result] 5 015 specimens of Syrphidae were cheaked in this study,and 141 species of 53 genera of 16 families in three subfamilies of flower-visiting species of Syrphidae were identified in farmland of Hanzhong area,in which 65 species were Syrphinae;72 species were Milesiinae;while four species were Microdontinae.The species of Syrphidae in this region were active in April,May,June and July.There were two dominant species in this region,including Episyrphus balteatus and Eristalis tenax.[Conclusion] This study had provided theoretical basis for the studies on the flower-visiting habit and fecundation of insects of Syrphidae in this area.
文摘[ Objective] The aim of this study was to isolate the endophyte of three solanaceae fruits and vegetables such as tomato, pepper and eggplant, to screen and identify the bacterial wilt antagonistic bacteria. [ Methodl According to the lapping liquid culture method, the endophyte of three plants was isolated by the selective medium and purified by the plate streaking method, so the purified enclophyte was screened by the hyphal pieces confront culture method. Furthermore, the screened antagonistic and endophyteic bacteria was identified and classified through culture characteristics of isolates and morphological features of thallus, Gram stain as well as physiological and biochemical reactions. [ Result] Fifty-three endophytic bacteria, fifty-three endophytic fungi and forty-four endophytic actinomycetes were separated from the endophyte of three plants. The screened fourteen endophytic bacteria with strong antagonistic effect on the bacterial wilt were classified to Bacillus, Escherichia, Klebsiella, Agromonas, Erwinia and Curto Bacterium respectively. Especially, Bacillus was the dominant species, which had the strongest antagonistic effect on the bacterial wilt. [ Conclusion] This study provides an effective way for biological control of the bacterial wilt in solanaceae.
基金Supported by the Science and Technology Project of Department of Education of Jiangxi Province(GJJ08399)~~
文摘[Objective] This study aimed to establish Balb/c mice model for food allergy caused by Chinese lobsters through using intraperitoneal injection for sensitization and explore methods for in vitro identification and evaluation of food allergy caused by Chinese lobsters. [Method] The 40 male Bal/c mice were divided into ovalbumin(OVA) positive control group, Coca's solution negative control group, blank control group and model group. Balb/c mice model was established by intraperitoneally injection of immunized Balb/c mice with OVA or Chinese lobster crude protein with aluminum hydroxide adjuvant. IgE and histamine levels in serum after the second challenge were determined by ELISA method, and the specific IgE antibody titer was determined by passive cutaneous anaphylaxis test(PCA); additionally, spleen index and histological changes in the small intestine, as well as food allergy symptoms after challenge were also calculated or observed. [Results] After the last challenge, IgE content was(236.75 ±73.39) μg/L in the Chinese lobster crude protein group, revealing no difference with that in the OVA group, but significantly higher than that in either the Coca's solution group or the blank control group(P 0.01);histamine content in serum in the Chinese lobster crude protein group was(406.55±232.79), significantly higher than that in the blank control group(P0.01). In the passive cutaneous anaphylaxis test, IgE antibody titer reached 1/16 after the last challenge in the Chinese lobster crude protein group. Spleen index in both Chinese lobster crude protein group and OVA group was significantly greater than that in either the Coca's solution group or the blank control group(P0.01). What's more, infiltration of inflammatory cells like lymphocytes and eosnophils at the lamina propria of intestinal mucosa was also observed both Chinese lobster crude protein group and OVA group. [Conclusion] This study established Balb/c mice model for food allergy caused by Chinese lobsters; serum IgE and ELISA assay and specific IgE antibody titer in PCA test can be used for the in vitro identification and evaluation of food allergies caused by Chinese lobsters.
文摘Food allergy is a common and increasing problem worldwide. The newly-found knowledge might provide novel experimental strategies, especially for laboratory diagnosis. Approximately 20% of the population alters their diet for a perceived adverse reaction to food, but the application of double-blind placebo-controlled oral food challenge, the "gold standard" for diagnosis of food allergy, shows that questionnaire-based studies overestimate the prevalence of food allergies. The clinical disorders determined by adverse reactions to food can be dassified on the basis of immunologic or nonimmunologic mechanisms and the organ system or systems affected. Diagnosis of food allergy is based on clinical history, skin prick tests, and laboratory tests to detect serum-food specific IgE, elimination diets and challenges. The primary therapy for food allergy is to avoid the responsible food. Antihistamines might partially relieve oral allergy syndrome and IgE-mediated skin symptoms, but they do not block systemic reactions. Systemic corticosteroids are generally effective in treating chronic IgE-mediated disorders. Epinephrine is the mainstay of treatment for anaphylaxis. Experimental therapies for IgE-mediated food allergy have been evaluated, such as humanized IgG anti-IgE antibodies and allergen specific immunotherapy.
基金Supported by the National High-technology Research and Development Program of China (863 Program), No. 2003AA302260
文摘AIM: To prepare a kind of magnetic iron-dextran nanopartides that was coated with anti-E.coli O157:H7 IgG, analyze its application conditions, and try to use it to isolate E.coli O157:H7 from foods. METHODS: Magnetic iron-dextran nanopartides were prepared by the reaction of a mixture of ferric and ferrous ions with dextran polymers under alkaline conditions. The particles were coated with antiserum against E.coli O157: H7 by the periodate oxidation-borohydride reduction procedure. The oxidation time, amount of antibody coating the particles, amount of nanoparticles, incubation time and isolation time were varied to determine their effects on recovery of the organisms. Finally, the optimum conditions for isolating E.coli O157:H7 from food samples were established. RESULTS: E.coli O157:H7 can be isolated from samples within 15 min with the sensitivity of 101 CFU/mL or even less. In the presence of 108 CFU/mL of other organisms, the sensitivity is 101-102 CFU/mL. Nonspecific binding of other bacteria to the particles was not observed. Two and a half hours of enrichment is enough for the particles to detect the target from the food samples inoculated with 1 CFU/g. CONCLUSION: Isolation of target bacteria by immuno magnetic nanoparticles is an efficient method with high sensitivity and specificity. The technique is so simple that it can be operated in lab and field even by untrained personnel.
基金the National Natural Science Foundation of China (59979029)
文摘A device to activate lignite and sludge by electro-hydraulic impulse is presented. It comprises an impulse current generator constituted by a high voltage transformer, a high voltage silicon rectifier, a current-limiting resistor, a capacitor bank and an air-break switch, and a lignite and sludge appliance made up of two plane electrodes and a working chamber. The installation activates the lignite or sludge delivered to the working chamber by the impulse current that is from the generator to puncture the main gap in the working chamber, raise the temperature therein up to (20 000 to 40 000) K and the energy density to as high as 109 Jm-3, and form a plasma piston. The alternative development and attenuation of plasma expansion makes the lignite in a number of physical and chemical processes resulting in diverse active radicals and ions, and also breaks it into grains mostly smaller than 250 mm. It is founded by experimental study that the technology can raise the content of nitro-nitrogen by 1.4 to 1.5 times and that of dissoluble organic substances by 5 to 10 times which is probably attributed to the decomposition of the ample germs in the lignite. The calculated power requirement to activate lignite is about (50 to 60) kWht-1. In comparison with muck applied to cucumber cultivation, the activated lignite demonstrates its features as an effective and economical green fertilizer by the same yield with half amount. These findings imply a promising access to green fertilizer.
文摘AIM: To explore the effect of Sinai san decoction on the development of non-alcoholic steatohepatitis induced by CCL4 combined with a fat-rich diet in rats.METHODS: Twenty-seven Sprague-Dawley rats were divided into three groups randomly: control group (n = 9),model group (n = 9) and treatment group (n = 9). The rats of model group and treatment group were given small dosage of CCL4 combined with a fat-rich diet, andthose of control group were given normal diet. After four weeks of fat-rich diet feeding, the rats of treatment group were given Sinai san decoction. The serum levels of aminotransferase and lipid were measured, and the pathology of livers was observed by HE staining after the rats were sacrificed at eight weeks.RESULTS: The rats' livers presented the pathology of steatosis and inflammation with higher serum levels of ALT and AST in the model group. In the treatment group the serum ALT and AST levels decreased significantly and were close to the control group. The hepatic inflammation scores also decreased markedly, but were still higher than those of control group. And the degree of hepatocyte steatosis was similar to that of model group.CONCLUSION: Sinai san decoction may ameliorate the hepatic inflammation of rats with steatohepatitis induced by small dosage of CCL4 combined with a fat-rich diet,but does not prevent the development of hepatocyte steatosis.
文摘Insects are major pests of stored grains. In sub Sahara Africa, stored grain insect pests are mainly Coleoptera or Lepidoptera. In the Logone Valley, area covering the Far North Region of Cameroon and South-West of Chad, to our knowledge, the diversity of insect pests noxious to stored products is not established. These beetles and weevils (Insecta:Coleoptera) during their development depreciate food in storage, were able to destroy a whole stock in a very short period of time if no protection of stored food is made. To achieve the diversity of insect pests, inspections of granaries were done in 209 villages of Logone Valley where insect pests were collected and identified. Secondly, the stored grains were sampled in peasant's granaries of Logone Valley and reared in incubators in the Unit of Entomological Research of the University of Ngaound6r6. After their emergence, insects were removed, collected and identified. Their noxious efficiency on stored grains is done by the calculation of the density of each insect pests per gram of grains. This study indicates that 12 species of harmful insects attack cereals and leguminous grains in the Logone Valley during storage. The Bruchids were the main insect pests of legumes. Callosobruchus maculatus was the main pest of legume and attacking mainly cowpeas (34.92 bruchids/g of seed) and peanuts (20.75 bruchids/g of seed). Seven harmful species destroy the cereals. Sitophilus zeamais is the main pest of maize (15.86 weevils/g of maize) and S. oryzae, for sorghum (36.59 weevils/g of maize). Tribolium castaneum were the main secondary pest. T. castaneum infests both cereals and leguminous. This work summarizes the importance and the diversification of stored grain pests in Logone Valley. Knowing the pest diversity is an important step in the strategy to develop a friendly and ecological control method of these harmful insects.
文摘This paper is divided into three subjects: Japanese Genghis Khan, Chinese tofu and Korean bibimbap (mixed rice with vegetables). It will be a cross-cultural and comparative research on historic foods among three countries (Japan, China, and Korea), in relation to national culinary identity.
基金Supported by the National Special Research Fund for Non-Profit Sector(Agriculture)(No.201203O81-7)
文摘Stable carbon and nitrogen isotopes were used to investigate nutrient pathways and trophic relationships from the rice-crab system in Panjin, Liaoning Province, China. Values of 313C ranged from -27.38%±0.44%o to -18.34%±0.26%o and δ^15N ranged from 1.10%o4-0.88%o to 9.33%±0.57%. Pseudorasbora parva (Stone moroko) had the highest δ^13C and 8tSN values. The lowest δ^13C values were obtained for the macrophytes and the lowest δ^15N value was found in sediments. Stable carbon and nitrogen isotopes were used to determine the contribution of different food items to the diets of crabs. The δ^13C results indicated that the Pseudorasbora parva made the greatest contribution to the diet of Eriocheir sinensis (Chinese mitten crab), while the δ^15N results indicated that most food items contributed more than 10% to the diet of the crab. There were three trophic levels identified in the system (Levels 0-2). The crab Eriocheir sinensis, fish Pseudorasbora parva and Misgurnus anguillicaudatus (Oriental weatherfish), and the oligochaete Limnodrilus hoffmeisteri (Limnodrilus worm), were at the second level, zooplankton were at the first level and suspended particulate matter and macrophytes were at trophic position 0.