通过不同功率和不同干燥时间对发芽糙米营养品质和食用性能影响的分析,探索发芽糙米微波干燥适宜工艺参数。结果表明,中火(464 W)干燥8 m in、中高火(648 W)干燥5 m in、高火(800 W)干燥4 m in均可达到发芽糙米安全贮藏水分的要求,且胶...通过不同功率和不同干燥时间对发芽糙米营养品质和食用性能影响的分析,探索发芽糙米微波干燥适宜工艺参数。结果表明,中火(464 W)干燥8 m in、中高火(648 W)干燥5 m in、高火(800 W)干燥4 m in均可达到发芽糙米安全贮藏水分的要求,且胶稠度均大于60 mm。其中,中高火干燥5 m in时,发芽糙米中还原糖、游离氨基酸、可溶性蛋白质和抗坏血酸的含量最高,糊化温度最低。展开更多
A versatile peroxidase (VP-Peco60-7 ) was generated and purified from the liquid culture of Pleurotus eryngii. The purification procedure included ammonium sulfate precipitation, ion exchange chromatography, and gel c...A versatile peroxidase (VP-Peco60-7 ) was generated and purified from the liquid culture of Pleurotus eryngii. The purification procedure included ammonium sulfate precipitation, ion exchange chromatography, and gel chromatography. The molecular weight and isoelectric point (pI) of VP-Peco60-7 were determined to be approxi-mately 40 kDa and 4.1, respectively. By N-terminal sequence determination and peptide mapping analysis, VP-Peco60-7 was found to be similar to the versatile peroxidase isoenzyme VPL1, which was previously isolated from liquid cultures of the same species. However, the molecular weight and pI of VP-Peco60-7 were different from those of versatile peroxidases of liquid cultures, implying that the VP-Peco60-7 in this study is of a novel type. With 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) as a substrate, the maximal enzyme activity was obtained at 50 °C and pH 3.0. The catalysis of ABTS by VP-Peco60-7 was expressed by the Michaelis-Menten equa-tion. At 50 °C and pH 3.0, the maximum velocity (V max ) was 188.68 U·mg-1 and the michaelis constant (K m ) was 203.09 μmol·L-1 .展开更多
Due to high price of Straight Vegetable Oil (SVO) for bio-diesel production, the use of Waste Cooking Oil (WCO) will be cost effective. Furthermore, utilization of WCO will refrain waterways pollution and endanger...Due to high price of Straight Vegetable Oil (SVO) for bio-diesel production, the use of Waste Cooking Oil (WCO) will be cost effective. Furthermore, utilization of WCO will refrain waterways pollution and endanger ecosystem. In Malaysia, more than 50-tone of WCO from various sources was produced every day. This study evaluates combustion performance and exhaust emission characteristics of several WCOs with different sources. Modification on fuel properties has been done to improve the combustion and exhaust emission of using WCO as diesel fuel. Regular diesel fuel also has been used for comparison in the test. A 0.6 liter, single-cylinder, air-cooled direct injection diesel engine was used to perform this experiment. Experiment was done at variable engine loads at constant speed.展开更多
An 8-week feeding experiment was conducted in an indoor recirculation seawater system to investigate the effects of partial replacement of dietary fishmeal with proteins from five sources on the growth performance and...An 8-week feeding experiment was conducted in an indoor recirculation seawater system to investigate the effects of partial replacement of dietary fishmeal with proteins from five sources on the growth performance and feed utilization of Sebastes schlegeli. Six isonitrogenous and isoenergetic diets were formulated using fishmeal(FM, the control) as sole protein source, or proteins from five sources including poultry by-product meal(PBM), meat and bone meal(MBM), soybean meal(SBM), cottonseed meal(CSM) and canola meal(CNM). Fifteen percent of the crude protein provided by fish meal was replaced, respectively. The results showed that the differences in specific growth rate(SGR) and survival rate(SR) among fish fed PBM, MBM, SBM, CSM and whole FM diets were not significant. However, SGR and SR of fish fed CNM diet was significantly lower than that of other treatments. Feeding rate, feed conversion, nutrient retention showed similar patterns to that of growth. Fish fed CSM and CNM showed significantly lower apparent digestibility coefficient(ADC) of dry matter and gross energy than those fed others while fish fed CNM showed lower ADC of crude protein than those fed others(P<0.05). These results suggested that it was feasible to substitute 15% dietary protein provided by fishmeal with PBM, MBM, SBM and CSM, respectively, but not with CNM as the replacement with CNM reduced fish growth and feed utilization.展开更多
SVO (straight vegetable oil) method means the direct use of vegetable oil as car fuel through installation of a heater unit in the car to decrease vegetable oil viscosity. In this study, the authors carried out perf...SVO (straight vegetable oil) method means the direct use of vegetable oil as car fuel through installation of a heater unit in the car to decrease vegetable oil viscosity. In this study, the authors carried out performance tests on the direct use of waste cooking oil using a car with a heater unit, Moreover, the authors carried out long run driving on road tests in five years using a public car of Minami-Aizu Town in Fukushima Prefecture and analyzed the case of troubles and clarified the availability and problems of SVO vehicles. As a result, the car with a heater unit shows similar performance in both cases using vegetable oil or diesel fuel as fuel. The tested SVO vehicle of Minami-Aizu Town could be driven 38,127 km mainly by waste vegetable oil with a total driving distance of 52,293 km in long run driving tests in five years, and decreased about 3,813.5 liters of light oil which corresponds to 9.99 t of CO2.展开更多
文摘通过不同功率和不同干燥时间对发芽糙米营养品质和食用性能影响的分析,探索发芽糙米微波干燥适宜工艺参数。结果表明,中火(464 W)干燥8 m in、中高火(648 W)干燥5 m in、高火(800 W)干燥4 m in均可达到发芽糙米安全贮藏水分的要求,且胶稠度均大于60 mm。其中,中高火干燥5 m in时,发芽糙米中还原糖、游离氨基酸、可溶性蛋白质和抗坏血酸的含量最高,糊化温度最低。
基金Supported by the Special Funds for Major State Basic Research Program of China (2007CB707805) the Natural Science Foundation of Zhejiang Province (Y505334)
文摘A versatile peroxidase (VP-Peco60-7 ) was generated and purified from the liquid culture of Pleurotus eryngii. The purification procedure included ammonium sulfate precipitation, ion exchange chromatography, and gel chromatography. The molecular weight and isoelectric point (pI) of VP-Peco60-7 were determined to be approxi-mately 40 kDa and 4.1, respectively. By N-terminal sequence determination and peptide mapping analysis, VP-Peco60-7 was found to be similar to the versatile peroxidase isoenzyme VPL1, which was previously isolated from liquid cultures of the same species. However, the molecular weight and pI of VP-Peco60-7 were different from those of versatile peroxidases of liquid cultures, implying that the VP-Peco60-7 in this study is of a novel type. With 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) as a substrate, the maximal enzyme activity was obtained at 50 °C and pH 3.0. The catalysis of ABTS by VP-Peco60-7 was expressed by the Michaelis-Menten equa-tion. At 50 °C and pH 3.0, the maximum velocity (V max ) was 188.68 U·mg-1 and the michaelis constant (K m ) was 203.09 μmol·L-1 .
文摘Due to high price of Straight Vegetable Oil (SVO) for bio-diesel production, the use of Waste Cooking Oil (WCO) will be cost effective. Furthermore, utilization of WCO will refrain waterways pollution and endanger ecosystem. In Malaysia, more than 50-tone of WCO from various sources was produced every day. This study evaluates combustion performance and exhaust emission characteristics of several WCOs with different sources. Modification on fuel properties has been done to improve the combustion and exhaust emission of using WCO as diesel fuel. Regular diesel fuel also has been used for comparison in the test. A 0.6 liter, single-cylinder, air-cooled direct injection diesel engine was used to perform this experiment. Experiment was done at variable engine loads at constant speed.
基金funded by National Key Basic Research Program (2009CB118702)partly by the Knowledge Innovation Program of the Chinese Academy of Sciences
文摘An 8-week feeding experiment was conducted in an indoor recirculation seawater system to investigate the effects of partial replacement of dietary fishmeal with proteins from five sources on the growth performance and feed utilization of Sebastes schlegeli. Six isonitrogenous and isoenergetic diets were formulated using fishmeal(FM, the control) as sole protein source, or proteins from five sources including poultry by-product meal(PBM), meat and bone meal(MBM), soybean meal(SBM), cottonseed meal(CSM) and canola meal(CNM). Fifteen percent of the crude protein provided by fish meal was replaced, respectively. The results showed that the differences in specific growth rate(SGR) and survival rate(SR) among fish fed PBM, MBM, SBM, CSM and whole FM diets were not significant. However, SGR and SR of fish fed CNM diet was significantly lower than that of other treatments. Feeding rate, feed conversion, nutrient retention showed similar patterns to that of growth. Fish fed CSM and CNM showed significantly lower apparent digestibility coefficient(ADC) of dry matter and gross energy than those fed others while fish fed CNM showed lower ADC of crude protein than those fed others(P<0.05). These results suggested that it was feasible to substitute 15% dietary protein provided by fishmeal with PBM, MBM, SBM and CSM, respectively, but not with CNM as the replacement with CNM reduced fish growth and feed utilization.
文摘SVO (straight vegetable oil) method means the direct use of vegetable oil as car fuel through installation of a heater unit in the car to decrease vegetable oil viscosity. In this study, the authors carried out performance tests on the direct use of waste cooking oil using a car with a heater unit, Moreover, the authors carried out long run driving on road tests in five years using a public car of Minami-Aizu Town in Fukushima Prefecture and analyzed the case of troubles and clarified the availability and problems of SVO vehicles. As a result, the car with a heater unit shows similar performance in both cases using vegetable oil or diesel fuel as fuel. The tested SVO vehicle of Minami-Aizu Town could be driven 38,127 km mainly by waste vegetable oil with a total driving distance of 52,293 km in long run driving tests in five years, and decreased about 3,813.5 liters of light oil which corresponds to 9.99 t of CO2.