OBJECTIVE:To combine calorie-restriction therapy(CRT) with Traditional Chinese Medicine(TCM) using Lingguizhugan Decoction and note the effects on expression of inflammatory cytokines[tumor necrosis factor(TNF)-α,hig...OBJECTIVE:To combine calorie-restriction therapy(CRT) with Traditional Chinese Medicine(TCM) using Lingguizhugan Decoction and note the effects on expression of inflammatory cytokines[tumor necrosis factor(TNF)-α,high-sensitivity C-reactive protein(hs-CRP),interleukin(IL)-6]in high-fat-diet induced hyperlipidemia in rats.METHODS:Forty rats were divided randomly and equally into four groups:control group A(regular feeding for 5 weeks);model group B(high-fat diet for 5 weeks),calorie-limited group C(high-fat diet for 5 weeks) and TCM calorie-limit group D(high-fat diet for 5 weeks).After modeling,groups C and D were fasted for 3 days:group C with simple fasting,and group D with TCM fasting.The motion as well as changes in color,body weight,food intake,plasma lipids[low-density lipoprotein-cholesterol(LDL-C),high-density lipoprotein-cholesterol,total cholesterol(TC) and triglyceride(TG) along with TNF-α,hs-CRP and IL-6]were measured before and after intervention.RESULTS:Modeled rats were established after five weeks.After 3 days of fasting,compared with group B,blood lipid levels(TG,TC,LDL-C) of groups C and D decreased dramatically.Those of group D decreased more obviously than those of group C.However,the values of TNF-α,hs-CRP and IL-6 showed no obvious difference between each of the three treatment groups.CONCLUSION:CRT can reduce fasting blood lipid levels in rats,but not by reducing the levels of inflammatory cytokines.展开更多
OBJECTIVE:To explore the effect of Linguizhugan decoction on hyperlipidemia rats with caloric restriction.METHODS:The hyperlipidemia model of rat was induced by high fat diet for 8 weeks.After the model was establishe...OBJECTIVE:To explore the effect of Linguizhugan decoction on hyperlipidemia rats with caloric restriction.METHODS:The hyperlipidemia model of rat was induced by high fat diet for 8 weeks.After the model was established,26 rats were randomly divided into 4 groups:the control group(n=6),the model group(n=6),the intermittent fasting(IF) group(n= 8),and the IF and herbal medicine(IFH) group(n= 6).IF group was applied intermittent fasting every other day.The IFH group was given Linguizhugan decoction every day and intermittent fasting every other day.Blood samples were taken at the end of 16 weeks,and serum ghrelin and lipid was tested.RESULTS:Serum ghrelin in the IF group significantly increased(P<0.01).Serum ghrelin in IFH group was lower than the IF group(P<0.05),but higher than the model group(P<0.01).CONCLUSION:Linguizhugan decoction may play a part in regulation of energy and appetite in hyperlipidemia rats with IF.展开更多
文摘OBJECTIVE:To combine calorie-restriction therapy(CRT) with Traditional Chinese Medicine(TCM) using Lingguizhugan Decoction and note the effects on expression of inflammatory cytokines[tumor necrosis factor(TNF)-α,high-sensitivity C-reactive protein(hs-CRP),interleukin(IL)-6]in high-fat-diet induced hyperlipidemia in rats.METHODS:Forty rats were divided randomly and equally into four groups:control group A(regular feeding for 5 weeks);model group B(high-fat diet for 5 weeks),calorie-limited group C(high-fat diet for 5 weeks) and TCM calorie-limit group D(high-fat diet for 5 weeks).After modeling,groups C and D were fasted for 3 days:group C with simple fasting,and group D with TCM fasting.The motion as well as changes in color,body weight,food intake,plasma lipids[low-density lipoprotein-cholesterol(LDL-C),high-density lipoprotein-cholesterol,total cholesterol(TC) and triglyceride(TG) along with TNF-α,hs-CRP and IL-6]were measured before and after intervention.RESULTS:Modeled rats were established after five weeks.After 3 days of fasting,compared with group B,blood lipid levels(TG,TC,LDL-C) of groups C and D decreased dramatically.Those of group D decreased more obviously than those of group C.However,the values of TNF-α,hs-CRP and IL-6 showed no obvious difference between each of the three treatment groups.CONCLUSION:CRT can reduce fasting blood lipid levels in rats,but not by reducing the levels of inflammatory cytokines.
基金Supported by International Science and Technology Cooperation Program of Guangdong Province (No.2009B050700022)
文摘OBJECTIVE:To explore the effect of Linguizhugan decoction on hyperlipidemia rats with caloric restriction.METHODS:The hyperlipidemia model of rat was induced by high fat diet for 8 weeks.After the model was established,26 rats were randomly divided into 4 groups:the control group(n=6),the model group(n=6),the intermittent fasting(IF) group(n= 8),and the IF and herbal medicine(IFH) group(n= 6).IF group was applied intermittent fasting every other day.The IFH group was given Linguizhugan decoction every day and intermittent fasting every other day.Blood samples were taken at the end of 16 weeks,and serum ghrelin and lipid was tested.RESULTS:Serum ghrelin in the IF group significantly increased(P<0.01).Serum ghrelin in IFH group was lower than the IF group(P<0.05),but higher than the model group(P<0.01).CONCLUSION:Linguizhugan decoction may play a part in regulation of energy and appetite in hyperlipidemia rats with IF.
