[Objective] The research aimed to optimize laboratory artificial rearing methods for Plutella xylostella L. and to select out effective insecticides against this pest species. [Method] Brassica chinensis seedlings wer...[Objective] The research aimed to optimize laboratory artificial rearing methods for Plutella xylostella L. and to select out effective insecticides against this pest species. [Method] Brassica chinensis seedlings were cultivated in the laboratory at （25±1）℃, 60-75% RH, and a photoperiod of 14：10 （L：D） h. The green blow molding papers were used to collect eggs, and Brassica oleracea seedlings were cultivated to breed Plutella xylostella L. until the insects were ready for use. Mean-while, the susceptibility of 3rd instar larvae of Plutella xylostella L. to five types of insecticides was tested by leaf-dipping method. [Result] Plutella xylostella L. larvae were found well-grown and uniform in developmental stages with average pupa weight of 5.4 mg, pupa emergence percentage of 94.0 % and egg number of 135.1 per female. The LCso values of 3rd instar Plutella xylostella L. larvae against 200 g/L chlorantraniliprole SC, 20% flubendiamide WG, 150 g/L indoxacarb EC, 200 g/L tebufenozide SC and 1.8% abamectin EC, were 0.152, 0.223, 1.151, 93.340 and 0.128 mg/L, respectively. [Conclusion] This rearing method is easy to be implemented and of great practicality, suitable for mass propagation of P. xylostella L. Abamectin, chlorantraniliprole and flubendiamide showed high insecticidal activities, so these three insecticides can be used alternately for the field prevention and control of Plutella xylostella L.展开更多
Two adult captive Malayan pangolin （Manis javanica） successfully mated on February 3, 2015, and gave birth to one healthy cub on April 8 in Pangolin＇s Breeding Center of College of Life Sciences, Chongqing Normal U...Two adult captive Malayan pangolin （Manis javanica） successfully mated on February 3, 2015, and gave birth to one healthy cub on April 8 in Pangolin＇s Breeding Center of College of Life Sciences, Chongqing Normal University. Current- ly, the two adults and the cub are in good state of health, and especially the cub is healthily growing according to its weight and other health indicators. This is the first birth and survival of cub of Malayan pangolins in captivity. It proves that Malayan pangolin can successfully breed in appropriate captivity conditions and the reproductive parameters of Malayan pangolin are also determined, which provide valuable reference for other species of pangolins in artificial breeding, and also give effective way for population growth, ecological restoration and species conservation for pangolin in the wild.展开更多
Since the 1990s, artificial propagation and breeding technique of marine fish in China have developed by way of increasing species and fry numbers, with special stress laid on valuable species. Large quantities of art...Since the 1990s, artificial propagation and breeding technique of marine fish in China have developed by way of increasing species and fry numbers, with special stress laid on valuable species. Large quantities of artificial fry can meet the needs of both marine cage culture and pond culture for most species. Experimental results obtained by scientists have been put into use in actual production. Fish fry production has entered a period of sustainable development. So far, at least 44 species (21 families) of marine fish have been successfully bred in China. The artificial fry number of large yellow croaker (Pseudosciaena crocea) exceeded 300 million in 1999. The species whose artificial fry numbers have each surpassed 10 million annually are red drum (Sciaenops ocellatus), Japanese seabass (Lateolabrax japonicus), cuneate drum (Nibea miichthioides), spring spawning red seabream (Pagrosomus major) and threebanded sweetlip (Plectorhynchus cinctus). Millions of artificial fry are bred annually in the species of black porgy (Sparus macrocephalus), Russell’s snapper (Lutjanus russelli), javelin grunt (Pomadasys hasta), miiuy croaker (Miichthys miiuy) and skewband grunt (Hapalogenys nitens). The fish in the family Sciaenidae are the main species in artificial propagation and breeding. Some problems and prospects on marine fish culture and stock enhancement are also discussed and some proposals for sustainable development are put forward in this article.展开更多
基金Supported by Key Technologies of R&D Program of Shanxi Province(20120311019-1)Research Project Supported by Shanxi Scholarship Council of China(2012-110)+1 种基金Doctoral Research Fund of Shanxi Academy of Agricultural Sciences(YBSJJ1006)Key Technologies of R&D Program of Shanxi Academy of Agricultural Sciences(2012ygg16)~~
文摘[Objective] The research aimed to optimize laboratory artificial rearing methods for Plutella xylostella L. and to select out effective insecticides against this pest species. [Method] Brassica chinensis seedlings were cultivated in the laboratory at （25±1）℃, 60-75% RH, and a photoperiod of 14：10 （L：D） h. The green blow molding papers were used to collect eggs, and Brassica oleracea seedlings were cultivated to breed Plutella xylostella L. until the insects were ready for use. Mean-while, the susceptibility of 3rd instar larvae of Plutella xylostella L. to five types of insecticides was tested by leaf-dipping method. [Result] Plutella xylostella L. larvae were found well-grown and uniform in developmental stages with average pupa weight of 5.4 mg, pupa emergence percentage of 94.0 % and egg number of 135.1 per female. The LCso values of 3rd instar Plutella xylostella L. larvae against 200 g/L chlorantraniliprole SC, 20% flubendiamide WG, 150 g/L indoxacarb EC, 200 g/L tebufenozide SC and 1.8% abamectin EC, were 0.152, 0.223, 1.151, 93.340 and 0.128 mg/L, respectively. [Conclusion] This rearing method is easy to be implemented and of great practicality, suitable for mass propagation of P. xylostella L. Abamectin, chlorantraniliprole and flubendiamide showed high insecticidal activities, so these three insecticides can be used alternately for the field prevention and control of Plutella xylostella L.
基金Supported by National Natural Science Foundation(31470570)Chongqing Natural Science Foundation(cstc2014jcyj A80013)+1 种基金Science Foundation of Chongqing Education Commission(kj1400534)Chongqing Creative Program for Graduate Students(CYS15155)~~
文摘Two adult captive Malayan pangolin （Manis javanica） successfully mated on February 3, 2015, and gave birth to one healthy cub on April 8 in Pangolin＇s Breeding Center of College of Life Sciences, Chongqing Normal University. Current- ly, the two adults and the cub are in good state of health, and especially the cub is healthily growing according to its weight and other health indicators. This is the first birth and survival of cub of Malayan pangolins in captivity. It proves that Malayan pangolin can successfully breed in appropriate captivity conditions and the reproductive parameters of Malayan pangolin are also determined, which provide valuable reference for other species of pangolins in artificial breeding, and also give effective way for population growth, ecological restoration and species conservation for pangolin in the wild.
文摘Since the 1990s, artificial propagation and breeding technique of marine fish in China have developed by way of increasing species and fry numbers, with special stress laid on valuable species. Large quantities of artificial fry can meet the needs of both marine cage culture and pond culture for most species. Experimental results obtained by scientists have been put into use in actual production. Fish fry production has entered a period of sustainable development. So far, at least 44 species (21 families) of marine fish have been successfully bred in China. The artificial fry number of large yellow croaker (Pseudosciaena crocea) exceeded 300 million in 1999. The species whose artificial fry numbers have each surpassed 10 million annually are red drum (Sciaenops ocellatus), Japanese seabass (Lateolabrax japonicus), cuneate drum (Nibea miichthioides), spring spawning red seabream (Pagrosomus major) and threebanded sweetlip (Plectorhynchus cinctus). Millions of artificial fry are bred annually in the species of black porgy (Sparus macrocephalus), Russell’s snapper (Lutjanus russelli), javelin grunt (Pomadasys hasta), miiuy croaker (Miichthys miiuy) and skewband grunt (Hapalogenys nitens). The fish in the family Sciaenidae are the main species in artificial propagation and breeding. Some problems and prospects on marine fish culture and stock enhancement are also discussed and some proposals for sustainable development are put forward in this article.
