[ Objective] The aim of this study was to lay a foundation for obtaining effective traditional Chinese medicine preparations controlling chicken colibacillosis. [ Method] With the western medicine Lomeflbxacin Hydroch...[ Objective] The aim of this study was to lay a foundation for obtaining effective traditional Chinese medicine preparations controlling chicken colibacillosis. [ Method] With the western medicine Lomeflbxacin Hydrochloride and Sodium Chloride Injection as the control, drug sensitivity tests of 15 single traditional Chinese herbs and seven self-made Chinese herbal compounds were conducted. Subsequently, the single traditional Chinese herbs presenting good in vitro antibacterial effects against Escherichia coli and all the seven compounds were given to the chicks artificially infected with E. coil to observe their efficacies. [ Result] The results of drug sensitivity tests showed that all the 15 traditional Chinese herbs had certain antibacterial effect against E. coli, among which aloe, pomegranate rind and radix sanguisorbae were the best. Compound 1, 2 and 5 had good antibacterial effects in vitro. By in vivo test, traditional Chinese medicines showed superior efficacies on infected chicks than western medicine, and compound 1 and 2 performed well. [ConclusiOn] Traditional Chinese medicine preparations are good at controlling Chicken colibacillosis, but their bacteriostasis mechanism in vivo was different with that in vitro.展开更多
[ Objective ] The aim of the study was to explore the resistance development of deltamethrin through selection of dettamethrin resistant strain of wild silkworm ( Bombyx Mandarina), thus providing the basis for scie...[ Objective ] The aim of the study was to explore the resistance development of deltamethrin through selection of dettamethrin resistant strain of wild silkworm ( Bombyx Mandarina), thus providing the basis for scientific pesticide application and resistance management. [ Method] The wild silkworms collected from three different regions were reared indoors, and the sensitivity of their parents to deltamethrin was detected by topical application. The larvae in each generation were treated with deltamethdn in median lethal dose or so by.topical application. The mortality of larva was analyzed for the establishment of toxicity regression equations and the calculation of the multiple or increased multiple of deltamethdn resistance.[Result] After the Qidong Bornbyx Mandarina (YQD) fed with mulberry leaves were selected for three generations indoors, the multiple of deltamethdn resistance of F, was 14.26, 1.2 times as great as that of Fo ; after the Bombyx Mandarina from mulberry garden of Soochow University (YSD) fed with mulberry leaves were selected for three generations indoors, the multiple of deltamethrin resistance of F4 was 16.48, 1.9 times as great as that of Fo ; after the Wujiang Bombyx Mandarina (YWJ) fed with artificial diets were selected for three generations indoors during six generations, the multiple of deltamethrin resistance of Fe was 18.67, 1.2 times as great as that of Fo. [ Conclusioa] With the selection in same dose, the resistance multiple of YSD increases more rapidly than that of YQD; under double selection of artificial diets and insecticide, the resistance multiple of YWJ increases more slowly than that of YQD.展开更多
基金Supported by Fund of Education Department in Hebei Province(2007408)~~
文摘[ Objective] The aim of this study was to lay a foundation for obtaining effective traditional Chinese medicine preparations controlling chicken colibacillosis. [ Method] With the western medicine Lomeflbxacin Hydrochloride and Sodium Chloride Injection as the control, drug sensitivity tests of 15 single traditional Chinese herbs and seven self-made Chinese herbal compounds were conducted. Subsequently, the single traditional Chinese herbs presenting good in vitro antibacterial effects against Escherichia coli and all the seven compounds were given to the chicks artificially infected with E. coil to observe their efficacies. [ Result] The results of drug sensitivity tests showed that all the 15 traditional Chinese herbs had certain antibacterial effect against E. coli, among which aloe, pomegranate rind and radix sanguisorbae were the best. Compound 1, 2 and 5 had good antibacterial effects in vitro. By in vivo test, traditional Chinese medicines showed superior efficacies on infected chicks than western medicine, and compound 1 and 2 performed well. [ConclusiOn] Traditional Chinese medicine preparations are good at controlling Chicken colibacillosis, but their bacteriostasis mechanism in vivo was different with that in vitro.
文摘[ Objective ] The aim of the study was to explore the resistance development of deltamethrin through selection of dettamethrin resistant strain of wild silkworm ( Bombyx Mandarina), thus providing the basis for scientific pesticide application and resistance management. [ Method] The wild silkworms collected from three different regions were reared indoors, and the sensitivity of their parents to deltamethrin was detected by topical application. The larvae in each generation were treated with deltamethdn in median lethal dose or so by.topical application. The mortality of larva was analyzed for the establishment of toxicity regression equations and the calculation of the multiple or increased multiple of deltamethdn resistance.[Result] After the Qidong Bornbyx Mandarina (YQD) fed with mulberry leaves were selected for three generations indoors, the multiple of deltamethdn resistance of F, was 14.26, 1.2 times as great as that of Fo ; after the Bombyx Mandarina from mulberry garden of Soochow University (YSD) fed with mulberry leaves were selected for three generations indoors, the multiple of deltamethrin resistance of F4 was 16.48, 1.9 times as great as that of Fo ; after the Wujiang Bombyx Mandarina (YWJ) fed with artificial diets were selected for three generations indoors during six generations, the multiple of deltamethrin resistance of Fe was 18.67, 1.2 times as great as that of Fo. [ Conclusioa] With the selection in same dose, the resistance multiple of YSD increases more rapidly than that of YQD; under double selection of artificial diets and insecticide, the resistance multiple of YWJ increases more slowly than that of YQD.