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饲料乳酸菌的分离鉴定及优良菌株筛选 被引量:6
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作者 热娜·米吉提 乌斯满·依米提 +4 位作者 周秀文 麦热姆妮萨·艾麦尔 李越中 买尔哈巴·艾合买提 古丽斯玛依·艾拜都拉 《生物技术通报》 CAS CSCD 北大核心 2012年第6期166-173,共8页
对从饲料玉米、高粱、麦秆及棉花中筛选出的乳酸菌进行分类鉴定和综合性分析。用MRS+CaCO3固体培养基从棉花中分离出乳酸菌18株、高粱中30株、饲料玉米中18株、麦秆中18株。经形态学、生理生化试验进行初步鉴定并按产酸试验,耐盐及耐酸... 对从饲料玉米、高粱、麦秆及棉花中筛选出的乳酸菌进行分类鉴定和综合性分析。用MRS+CaCO3固体培养基从棉花中分离出乳酸菌18株、高粱中30株、饲料玉米中18株、麦秆中18株。经形态学、生理生化试验进行初步鉴定并按产酸试验,耐盐及耐酸试验挑选出32株产酸率强的乳酸菌对其进行16S rDNA分子鉴定。结果显示,32株菌都具有良好的耐盐、耐酸能力;经生理生化和16S rDNA基因序列鉴定可知32株乳酸菌分属于两个属,即乳杆菌属、肠球菌属,4个种,即干酪乳杆菌(Lactobacilluscasei)、肠道球菌(Entercoccus faecium)、植物乳杆菌(Lactobacillus plantarum)、海氏肠球菌(Entercoccus hirae)。4种饲料原料中肠道球菌普遍存在。除了这种乳酸菌以外,棉花有干酪乳杆菌、植物乳杆菌、海氏肠球菌,玉米和麦秆内有植物乳杆菌。从饲料中筛选出4株具有较强产酸能力的乳酸菌,可进一步研发成青贮饲料添加剂。 展开更多
关键词 饲料玉米 饲料棉花 饲料高粱 饲料麦秆 乳酸菌 分离 鉴定 16S rDNA
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Feeding Effect of Triticale Fodder as Replacement of Straw on Production Performance of Dairy Cows 被引量:2
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作者 Nathu Ram Sarker Mohammad Asaduzzaman +4 位作者 Khan Shahidul Huque Mohammad Toyebur Rahman NazrulIslam Mohammad Enamul Haque Stephen R. Waadington 《Journal of Agricultural Science and Technology(A)》 2013年第1期72-82,共11页
Twenty lactating cows of two to five parity having an average live weight of 290.00 to 330.00 kg and an average milk production of 4.54 kg/head/day to 4.66 kg/head/day were selected from the Central Cattle breeding St... Twenty lactating cows of two to five parity having an average live weight of 290.00 to 330.00 kg and an average milk production of 4.54 kg/head/day to 4.66 kg/head/day were selected from the Central Cattle breeding Station and Dairy Farm. The four dietary treatment were: SlooTo (Straw 100: Triticale 0 as control); $5oT5o (Straw 50: Triticale 50); $25T75 (Straw 25: Triticale 75) and SoTloo (Straw 0: Triticale 100). It was observed that the roughage dry matter intake (DMI) (2.61 ~ 0.07 kg) in percent live weight was significantly (P 〈 0.05) higher in $25T75 and the lowest (1.76 ~ 0.018 kg) in the control group (SlooTo) and the differences were significant among the dietary treatments except $5oT5o and SoTloo. The total DMI was significantly (P 〈 0.05) higher in SoT^oo (13.36 i 0.13 kg) followed by $25T75, $5oT5o and SlooTo, respectively. The digestibility of crude protein (CP) was slightly higher in $25T75 (75.48 ~ 0.96) compared to SoTtoo (75.31 ~ 1.45) and the difference was non-significant (P 〉 0.05). Milk production was significantly (P 〈 0.05) the highest in $5oT5o and the lowest in SlooTo followed by SoTloo, $25T75, respectively. The percent increased in milk yield was also the highest in $5oT5o (67.68%) and the lowest in SlooTo (28.85%). The 4% fat corrected milk was also significantly (P 〈 0.05) higher in $5oT5o followed by SoTloo, $25T75 and SlooTo, respectively. Therefore, it can be concluded that triticale and straw at a ratio of 50:50 may be fed for better production performance of dairy cows. 展开更多
关键词 Triticale green fodder production performance REPLACEMENT milk yield.
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