The unsaponifiable compounds derived from the fresh green beans of Vanilla siamens＆ Rol. ex. Dow were assayed for the first time to detect their estrogenic activity. We used a simple screening method using the yeast ...The unsaponifiable compounds derived from the fresh green beans of Vanilla siamens＆ Rol. ex. Dow were assayed for the first time to detect their estrogenic activity. We used a simple screening method using the yeast two hybrid system based on the binding of a ligand to estrogen receptors. Yeast cells carrying the hER （human estrogen receptor） gene, ERE （estrogen response elements） and lacZ （β-galactosidase gene） are very suitable for screening and sensitive analysis of estrogenic compound. Our results showed that V. siamensis plant extracts bind with relatively affinity to YES- hERa was 2.27-fold the relative potency ofestradiol （E2） in YES-hERa. The effects of phytoestrogen activity on the osteoblast cells were examined on the proliferation of hFOB 1.19 cells and the bone mineralization process. V. siamens＆ was a positive screening result and induced mineralization ofosteoblasts. This study indicated that V. siamensis plant extract exhibited the characteristic effects of a nature bone promoter compound as phytoestrogen.展开更多
文摘The unsaponifiable compounds derived from the fresh green beans of Vanilla siamens＆ Rol. ex. Dow were assayed for the first time to detect their estrogenic activity. We used a simple screening method using the yeast two hybrid system based on the binding of a ligand to estrogen receptors. Yeast cells carrying the hER （human estrogen receptor） gene, ERE （estrogen response elements） and lacZ （β-galactosidase gene） are very suitable for screening and sensitive analysis of estrogenic compound. Our results showed that V. siamensis plant extracts bind with relatively affinity to YES- hERa was 2.27-fold the relative potency ofestradiol （E2） in YES-hERa. The effects of phytoestrogen activity on the osteoblast cells were examined on the proliferation of hFOB 1.19 cells and the bone mineralization process. V. siamens＆ was a positive screening result and induced mineralization ofosteoblasts. This study indicated that V. siamensis plant extract exhibited the characteristic effects of a nature bone promoter compound as phytoestrogen.
