本研究旨在探讨灵香草提取物中的主要活性成分Foenumoside B (FB)与有氧运动(AE)协同对高脂血症大鼠肝脏脂质代谢紊乱的影响。通过建立高脂饮食诱导的高脂血症大鼠模型,将大鼠随机分为对照组、HLP组、AE组、FB组和FB+AE联合组,通过实验...本研究旨在探讨灵香草提取物中的主要活性成分Foenumoside B (FB)与有氧运动(AE)协同对高脂血症大鼠肝脏脂质代谢紊乱的影响。通过建立高脂饮食诱导的高脂血症大鼠模型,将大鼠随机分为对照组、HLP组、AE组、FB组和FB+AE联合组,通过实验观察各组大鼠的血脂水平、肝脏脂质代谢相关基因表达以及肝脏组织病理变化。结果表明,与单独使用FB或AE相比,FB与AE的联合干预能更有效地降低血脂水平,调节ACAT1、DGAT2、FAS、SREBP1c、PPARα、LCAT、UCP2、MCD和CPT-1等脂质代谢相关基因的表达,减轻肝脏脂质积累和损伤。本研究证实了FB与AE的协同干预对改善HLP大鼠的脂质代谢紊乱具有显著效果,为开发新型的HLP治疗策略提供了科学依据。展开更多
We determined the global yield and composition of extracts from vetiver roots cultivated hydroponically. We extracted roots of two varieties collected at different times after planting. The extracts were obtained by s...We determined the global yield and composition of extracts from vetiver roots cultivated hydroponically. We extracted roots of two varieties collected at different times after planting. The extracts were obtained by supercritical fluid extraction （SFE）; we also estimated manufacturing costs. Constituents were quantified by gas-chromatography/time ionization detection （GC-FID）. Results demonstrated the efficacy of a hydroponic system in producing vctiver with a high root extract content. The largest yield was obtained from roots collected on days 132 and 92 after planting from varieties National （1.41%） and Bourbon （1.28%）, respectively. The major compounds present in the extract were khusimoi, isovalencenol, alpha-vetivone and beta-vetivone. To determine the economic viability of producing these extracts, manufacturing costs were estimated using a simplified methodology. The manufacturing costs were US$ 22.26/kg and US$ 24.26/kg for varieties national and Bourbon, respectively.展开更多
The unsaponifiable compounds derived from the fresh green beans of Vanilla siamens＆ Rol. ex. Dow were assayed for the first time to detect their estrogenic activity. We used a simple screening method using the yeast ...The unsaponifiable compounds derived from the fresh green beans of Vanilla siamens＆ Rol. ex. Dow were assayed for the first time to detect their estrogenic activity. We used a simple screening method using the yeast two hybrid system based on the binding of a ligand to estrogen receptors. Yeast cells carrying the hER （human estrogen receptor） gene, ERE （estrogen response elements） and lacZ （β-galactosidase gene） are very suitable for screening and sensitive analysis of estrogenic compound. Our results showed that V. siamensis plant extracts bind with relatively affinity to YES- hERa was 2.27-fold the relative potency ofestradiol （E2） in YES-hERa. The effects of phytoestrogen activity on the osteoblast cells were examined on the proliferation of hFOB 1.19 cells and the bone mineralization process. V. siamens＆ was a positive screening result and induced mineralization ofosteoblasts. This study indicated that V. siamensis plant extract exhibited the characteristic effects of a nature bone promoter compound as phytoestrogen.展开更多
文摘本研究旨在探讨灵香草提取物中的主要活性成分Foenumoside B (FB)与有氧运动(AE)协同对高脂血症大鼠肝脏脂质代谢紊乱的影响。通过建立高脂饮食诱导的高脂血症大鼠模型,将大鼠随机分为对照组、HLP组、AE组、FB组和FB+AE联合组,通过实验观察各组大鼠的血脂水平、肝脏脂质代谢相关基因表达以及肝脏组织病理变化。结果表明,与单独使用FB或AE相比,FB与AE的联合干预能更有效地降低血脂水平,调节ACAT1、DGAT2、FAS、SREBP1c、PPARα、LCAT、UCP2、MCD和CPT-1等脂质代谢相关基因的表达,减轻肝脏脂质积累和损伤。本研究证实了FB与AE的协同干预对改善HLP大鼠的脂质代谢紊乱具有显著效果,为开发新型的HLP治疗策略提供了科学依据。
文摘We determined the global yield and composition of extracts from vetiver roots cultivated hydroponically. We extracted roots of two varieties collected at different times after planting. The extracts were obtained by supercritical fluid extraction （SFE）; we also estimated manufacturing costs. Constituents were quantified by gas-chromatography/time ionization detection （GC-FID）. Results demonstrated the efficacy of a hydroponic system in producing vctiver with a high root extract content. The largest yield was obtained from roots collected on days 132 and 92 after planting from varieties National （1.41%） and Bourbon （1.28%）, respectively. The major compounds present in the extract were khusimoi, isovalencenol, alpha-vetivone and beta-vetivone. To determine the economic viability of producing these extracts, manufacturing costs were estimated using a simplified methodology. The manufacturing costs were US$ 22.26/kg and US$ 24.26/kg for varieties national and Bourbon, respectively.
文摘The unsaponifiable compounds derived from the fresh green beans of Vanilla siamens＆ Rol. ex. Dow were assayed for the first time to detect their estrogenic activity. We used a simple screening method using the yeast two hybrid system based on the binding of a ligand to estrogen receptors. Yeast cells carrying the hER （human estrogen receptor） gene, ERE （estrogen response elements） and lacZ （β-galactosidase gene） are very suitable for screening and sensitive analysis of estrogenic compound. Our results showed that V. siamensis plant extracts bind with relatively affinity to YES- hERa was 2.27-fold the relative potency ofestradiol （E2） in YES-hERa. The effects of phytoestrogen activity on the osteoblast cells were examined on the proliferation of hFOB 1.19 cells and the bone mineralization process. V. siamens＆ was a positive screening result and induced mineralization ofosteoblasts. This study indicated that V. siamensis plant extract exhibited the characteristic effects of a nature bone promoter compound as phytoestrogen.
