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香蕉多酚氧化酶的纯化、酶学性质及活性抑制的研究进展 被引量:4
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作者 袁德保 杨昭 +5 位作者 李芬芳 谭琳 李奕星 陈娇 郑晓燕 郑丽丽 《食品科学》 EI CAS CSCD 北大核心 2013年第19期330-335,共6页
多酚氧化酶(PPO)是引起香蕉酶促褐变的关键酶。在香蕉采后及加工过程中,PPO与香蕉的品质有重要关系。本文综述香蕉PPO的分离纯化、酶学性质以及活性抑制等方面的研究进展,以期为香蕉加工科研工作提供一定的借鉴。
关键词 香蕉多酚氧化酶 纯化 特性 活性抑制
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聚马来松香酰乙二胺-Cu(Ⅱ)配合物的合成及在固定化酶中的应用 被引量:2
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作者 曹宇 史伯安 《化学与生物工程》 CAS 2007年第5期60-63,共4页
介绍了聚马来松香酰乙二胺-Cu(Ⅱ)高分子配合物的制备和固定化香蕉多酚氧化酶的方法,测定了固定化香蕉多酚氧化酶的活性和米氏常数,研究了温度和pH值对固定化香蕉多酚氧化酶催化活性的影响及固定化香蕉多酚氧化酶的重复催化活性。结果表... 介绍了聚马来松香酰乙二胺-Cu(Ⅱ)高分子配合物的制备和固定化香蕉多酚氧化酶的方法,测定了固定化香蕉多酚氧化酶的活性和米氏常数,研究了温度和pH值对固定化香蕉多酚氧化酶催化活性的影响及固定化香蕉多酚氧化酶的重复催化活性。结果表明,聚马来松香酰乙二胺-Cu(Ⅱ)高分子配合物,是香蕉多酚氧化酶的良好固定化载体,固定化香蕉多酚氧化酶的米氏常数Km=4.56×10-3mol.L-1,小于天然香蕉多酚氧化酶的米氏常数(1.95×10-2mol.L-1),固定化香蕉多酚氧化酶的耐热性和耐酸碱性均优于天然香蕉多酚氧化酶,并有一定的重复使用性。对香蕉多酚氧化酶的固定化方式和固定化香蕉多酚氧化酶的催化反应机理也进行了初步探讨。 展开更多
关键词 聚马来松香酰乙二胺 Cu(Ⅱ)-高分子配合物载体 香蕉多酚氧化酶 固定化
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Study on Processing of Clear Banana Juice Using Hot Water Extraction Method 被引量:2
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作者 郑欣 余元善 +4 位作者 吴继军 徐玉娟 肖更生 黄婉华 温靖 《Agricultural Science & Technology》 CAS 2014年第11期2003-2005,2044,共4页
In this study, the effects of hot water temperature on clarity, total soluble solids, polyphenol oxidase (PPO) and color of banana juice during hot water ex-traction were discussed based on the theory in which heat ... In this study, the effects of hot water temperature on clarity, total soluble solids, polyphenol oxidase (PPO) and color of banana juice during hot water ex-traction were discussed based on the theory in which heat treatment might induce the pectin and protein in banana pulp to form insoluble products. The results showed the hot water temperature had a significant effect on the formation of insol-uble polymers in banana pulp from pectin and protein. In 75 ℃ water, the pectin and protein in banana pulp were most inclined to form insoluble products. Under this condition, the clarity of banana juice was also highest. The light transmittance at 660 nm was close to 90%. In the banana juice, extracted by 75 ℃ water, the pectin and protein contents were lowest, and they were lower than 7.3 mg/100 ml and 12.9 mg/100 ml respectively. The 75 ℃ water could not inactivate completely the pectin in banana pulp due to its high heat resistance, Therefore, 0.05% L-cys-teine or ascorbic acid needed to be added into banana pulp to inhibit the browning of juice induced by residual PPO. 展开更多
关键词 Clear banana juice Hot water extraction Polyphenol oxidase Pectin Protein
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