The research was done at Havana Biofabric, Mayabeque Province. The vitro plants of banana （Musa sp） used came from the clone Enano Guantanamero （viand type）. They come from multiplication phase in a Murashige and ...The research was done at Havana Biofabric, Mayabeque Province. The vitro plants of banana （Musa sp） used came from the clone Enano Guantanamero （viand type）. They come from multiplication phase in a Murashige and MS （Skoog medium）. The objective of this work was to study the potentialities of HA （humic acids） for stimulation of rooting and cell division process, searching for completely synthetic hormone substitution due to its high cost. The multiplication medium contained thiamine （2 mg·L^-1）, myo-inositol （100 mg·L^-1）, sugar （30mg·L^-1）. By the use of HA, five treatments were used with total substitution of auxin and cytokinin （TI： HA （10 mg.Ll）; （T2： HA （20 mg·L^-1）; （T3： HA （30mg·L^-1）; （T4： HA （40 mg·L^-1）; （T5： HA （50 mg·L^-1） and the control with 6BAP （benzil amino purine） （4 mg·L^-1） ＋ IBA （indol butyric acid） （0.65 mg·L^-1）, pH of the medium was adjust just before agar were add. The results showed that in the explants under HA treatments, elongation and cell multiplication were favored confirmed by explants great high, root number and length due to dry mass. The total protein content, reduced carbohydrate and peroxidases enzymatic activity were determine. The use of Vermicompost HA in the in vitro micropropagation of banana allow the elimination of rooting phase saving materials and be able to pass explants directly to Acclimatization phase.展开更多
文摘The research was done at Havana Biofabric, Mayabeque Province. The vitro plants of banana （Musa sp） used came from the clone Enano Guantanamero （viand type）. They come from multiplication phase in a Murashige and MS （Skoog medium）. The objective of this work was to study the potentialities of HA （humic acids） for stimulation of rooting and cell division process, searching for completely synthetic hormone substitution due to its high cost. The multiplication medium contained thiamine （2 mg·L^-1）, myo-inositol （100 mg·L^-1）, sugar （30mg·L^-1）. By the use of HA, five treatments were used with total substitution of auxin and cytokinin （TI： HA （10 mg.Ll）; （T2： HA （20 mg·L^-1）; （T3： HA （30mg·L^-1）; （T4： HA （40 mg·L^-1）; （T5： HA （50 mg·L^-1） and the control with 6BAP （benzil amino purine） （4 mg·L^-1） ＋ IBA （indol butyric acid） （0.65 mg·L^-1）, pH of the medium was adjust just before agar were add. The results showed that in the explants under HA treatments, elongation and cell multiplication were favored confirmed by explants great high, root number and length due to dry mass. The total protein content, reduced carbohydrate and peroxidases enzymatic activity were determine. The use of Vermicompost HA in the in vitro micropropagation of banana allow the elimination of rooting phase saving materials and be able to pass explants directly to Acclimatization phase.
