赝靛及其制剂中马卡因(Maackiain)的薄层扫描含量测定

目的 :采用薄层色谱扫描法测定赝靛及其制剂施保利通口服液 (EsberitoxN)中指标成分马卡因 (Maackiain)的含量 ,为了考察不同色谱法定量测定的相关性 ,与高效液相色谱法测得的结果进行比较。方法 :样品经预处理后点于硅胶6 0F2 54 预制板上 ,以甲苯 丙酮 (8∶2 )为展开剂 ,于 30 6nm波长处进行吸收扫描 ,所得结果与HPLC测得的结果比较。结果 :此方法的平均回收率为 99.7% ,最低检测限为 0 .2 4μg ,符合中草药制剂的定量要求。 结论 :TLCS法与HPLC法比较 ,测定结果相关性良好。薄层扫描法和高效液相色谱法是互为补充的两种不同体系的色谱法 。

基于UPLC-Q-TOF-MS分析黄酮类成分马卡因在大鼠体内的代谢产物及代谢途径

采用超高效液相色谱-四极杆飞行时间质谱联用技术(UPLC-Q-TOF-MS),基于UNIFI数据分析处理软件的预测功能,结合肝微粒体体外孵育平台,对黄酮类成分马卡因在大鼠体内的代谢产物进行研究。推测了马卡因经大鼠口服吸收后在体内代谢后的10个产物。研究结果表明,马卡因在体内的生物转化过程主要经历Ⅰ相氧化、脱氢反应,Ⅱ相硫酸结合、葡萄糖结合和葡萄糖醛酸结合反应。其中,发生葡萄糖结合的代谢产物三叶豆紫檀苷,经对照品比对首次得到了确定。通过体外代谢研究结果进一步证实了马卡因在大鼠体内的代谢产物及其代谢途径。血、尿和粪中的代谢产物互为补充,系统全面地揭示了马卡因在血中的移行和代谢及排泄方式。该研究结果为马卡因体内代谢机制的进一步研究,以及药效和毒性作用机制的深入探究奠定了基础。

一测多评法同时测定开喉剑喷雾剂(儿童型)中4种成分

目的 建立一测多评法同时测定开喉剑喷雾剂(儿童型)(八爪金龙、山豆根、蝉蜕等)中4种成分的含量。方法 该药物的分析采用Platisil-ODS色谱柱(250 mm×4.6 mm,5μm);流动相甲醇-0.1%磷酸(三乙胺调节pH至5.0);体积流量0.8 mL/min;柱温35℃;检测波长220、275、300 nm。以岩白菜素为内标,计算其他3种成分的相对校正因子,测定其含量。结果 苦参碱、岩白菜素、三叶豆紫檀苷、马卡因分别在56.73~496.37、64.19~561.68、8.29~72.52、1.96~17.19μg/mL范围内线性关系良好(r≥0.999 6),平均加样回收率分别为99.89%、96.77%、92.12%、97.29%,RSD分别为1.57%、1.98%、0.96%、1.36%。一测多评法所得结果与外标法接近。结论 该方法简便可行,准确可靠,可用于开喉剑喷雾剂(儿童型)的质量控制。

山羊脊髓麻醉的实验研究

给山羊蛛网膜腔注射5%的利多卡因或1%的马卡因后进行了脊髓麻醉的研究。观察到腰荐区、会阴和尾的直接麻醉。在麻醉中,出现犬坐势,在恢复中看到膝、跗关节屈曲姿势。麻醉的持续时间在马卡因组几乎比利多卡因组长一倍。麻醉的前15分钟,两组的体温都很明显的降低。在注射后的前30分钟,两组的呼吸数都明显减少,随后稳定下来。而在利多卡因组,注射后20分钟,马卡因组注射后45分钟,脉数显著增加。

The effects of local anesthetics on intracellular Ca2+ release from ryanod ine-sensitive Ca2+ stores in gerbil hippocampal neurons

OBJECTIVE: To examine the effects of procaine and lidocaine on intracellular Ca(2+) release from sarcoplasmic reticulum ryanodine-sensitive Ca(2+) stores. METHODS: The experiment was performed on hippocampal slices from 60-80 g male Mongolian gerbils. Levels of intracellular Ca(2+) concentration in the slices were measured by microfluorometry. The slices were perfused with 50 mmol/L KCl containing medium for 30 seconds. Then, the medium was switched to physiological medium. After 5 min of incubation, the slice was perfused with 20 mmol/L caffeine containing physiology medium for 2 min. Following incubation, the slice was superfused with physiological medium until the end of the experiment. The effects of procaine and lidocanin (100 micro mol/L) on caffeine-evoked Ca(2+) release were evaluated by adding them to the medium after high K(+) medium perfusion. RESULTS: Caffeine induced a marked increase in intracellular Ca(2+) concentration which was then decreased 12% upon the addition of procaine (P

The effects of local anesthetics on intracellular Ca^(2+) release from ryanodine-sensitive Ca^(2+) stores in gerbil hippocampal neurons

To examine the effects of procaine and lidocaine on intracellular Ca 2+ release from sarcoplasmic reticulum ryanodine sensitive Ca 2+ stores Methods The experiment was performed on hippocampal slices from 60-80 g male Mongolian gerbils Levels of intracellular Ca 2+ concentration in the slices were measured by microfluorometry The slices were perfused with 50 mmol/L KCl containing medium for 30 seconds Then, the medium was switched to physiological medium After 5 min of incubation, the slice was perfused with 20 mmol/L caffeine containing physiology medium for 2 min Following incubation, the slice was superfused with physiological medium until the end of the experiment The effects of procaine and lidocanin (100 μmol/L) on caffeine evoked Ca 2+ release were evaluated by adding them to the medium after high K + medium perfusion Results Caffeine induced a marked increase in intracellular Ca 2+ concentration which was then decreased 12% upon the addition of procaine ( P 【0 05); however, lidocaine, did not induce a similar inhibitory reaction Conclusion Procaine inhibits ryanodine receptor mediated Ca 2+ release from intracellular Ca 2+ stores, while lidocaine may inhibit Ca 2+ release through other mechanisms