全名为大卫·艾伦·马梅特(David Alan Mamet,1947-),他生于美国伊利诺伊州的芝加哥市,曾在佛蒙特州普莱恩菲尔德市就读于戈达德学院,1969年获得了文学学士学位。他在求学期间就开始创作剧本,以创作绝望的工人阶级成员和设计粗...全名为大卫·艾伦·马梅特(David Alan Mamet,1947-),他生于美国伊利诺伊州的芝加哥市,曾在佛蒙特州普莱恩菲尔德市就读于戈达德学院,1969年获得了文学学士学位。他在求学期间就开始创作剧本,以创作绝望的工人阶级成员和设计粗俗独特的对话著称。回乡后,他在不同工厂工作过,还曾任职于一家房地产中介公司,也当过出租车司机,这些经历为他的戏剧创作提供了许多素材。1973年,他在芝加哥与人合伙创办了一家戏剧公司,并在美国的几所学院和大学教授戏剧课程,日后他有不少剧作是在芝加哥上演。展开更多
A study was conducted on the identifications of the degraded samples of sika deer （Cervus nippon） and red deer （Cervus elaphus） by phylogenetic and nucleotide distance analysis of partial Cytb and 12s rRNA genes s...A study was conducted on the identifications of the degraded samples of sika deer （Cervus nippon） and red deer （Cervus elaphus） by phylogenetic and nucleotide distance analysis of partial Cytb and 12s rRNA genes sequences. 402 bp Cytb genes were achieved by PCR-sequencing using DNA extracted from 8 case samples, and contrasted with 27 sequences of Cytb gene downloaded from GenBank database. The values of three nucleotide distance between three suspected samples and sika deer were identical （0.026±0.006）, which was smaller than the smallest nucleotide distance between eastern red deer and sika deer （0.036）. Furthermore, phylogenetic analysis of sika deer and red deer indicated that the evidences located within the same cluster as sika deer. The evidences were sika deer materials. As the same way, other three suspected samples were derived from red deer. The results were further confirmed by phylogenetic and nucleotide distance analysis of 387 bp 12s rRNA gene. The method was powerful and less time-consuming and helpful to reduce the related cases with wildlife.展开更多
文摘全名为大卫·艾伦·马梅特(David Alan Mamet,1947-),他生于美国伊利诺伊州的芝加哥市,曾在佛蒙特州普莱恩菲尔德市就读于戈达德学院,1969年获得了文学学士学位。他在求学期间就开始创作剧本,以创作绝望的工人阶级成员和设计粗俗独特的对话著称。回乡后,他在不同工厂工作过,还曾任职于一家房地产中介公司,也当过出租车司机,这些经历为他的戏剧创作提供了许多素材。1973年,他在芝加哥与人合伙创办了一家戏剧公司,并在美国的几所学院和大学教授戏剧课程,日后他有不少剧作是在芝加哥上演。
文摘A study was conducted on the identifications of the degraded samples of sika deer （Cervus nippon） and red deer （Cervus elaphus） by phylogenetic and nucleotide distance analysis of partial Cytb and 12s rRNA genes sequences. 402 bp Cytb genes were achieved by PCR-sequencing using DNA extracted from 8 case samples, and contrasted with 27 sequences of Cytb gene downloaded from GenBank database. The values of three nucleotide distance between three suspected samples and sika deer were identical （0.026±0.006）, which was smaller than the smallest nucleotide distance between eastern red deer and sika deer （0.036）. Furthermore, phylogenetic analysis of sika deer and red deer indicated that the evidences located within the same cluster as sika deer. The evidences were sika deer materials. As the same way, other three suspected samples were derived from red deer. The results were further confirmed by phylogenetic and nucleotide distance analysis of 387 bp 12s rRNA gene. The method was powerful and less time-consuming and helpful to reduce the related cases with wildlife.
