银白杨×马氏杨杂交子代及亲本的AFLP分析

为了探讨银白杨、马氏杨及银白杨×马氏杨杂交子代间的遗传多样性参数及个体聚类关系,利用8对引物对银白杨、马氏杨及银白杨×马氏杨杂交子代4个样本进行了AFLP分析。结果表明,8对引物共扩增出780条扩增谱带,其中多态性条带数为715条,多态性比例为91.65%,平均有效等位基因数为1.5017,Nei’s平均遗传多样性指数为0.3065,Shannon平均信息指数为0.4679。不同杂交子代在E-AAC/M-CAG引物对上均有特异性位点,这些特异位点可用于品种鉴定。聚类研究发现,在相似系数为0.76时,试验样品分成2个AFLP群,其中马氏杨为1个AFLP群,银白杨及2个杂交子代为1个AFLP群;当相似系数为0.78时,银白杨和2个杂交子代的AFLP群又划分为2个亚群,其中杂交子代1和杂交子代2聚合为亚群1,银白杨为亚群2。本研究的结果多态性比例很高,对样品的区分率达到100%,为杨树AFLP分析检测提供了指导借鉴价值。

Redescription of male and female genitalia of Tipula(Emodotipula) holoteles(Diptera), with notes on the structure of the semen pump in the subgenus Emodotipula

The original description of male hypopygium of Tipula(Emodotipula) holoteles Alexander, 1924 is expanded with additional morphological characters and detailed illustrations. The additional morphological descriptions of the female ovipositor along with detailed drawings are provided. The male semen pump and the female vaginal apodeme as well as the spermatheca are also described and illustrated for the first time, representing the first report of these structures of Tipula(Emodotipula) holoteles. Additionally, the taxonomic potential of the semen pump in the subgenus Emodotipula is analyzed and discussed. The new specific name Tipula(Emodotipula) yangi nom. nov. is proposed as a replacement name for junior homonym Tipula(Emodotipula) alexanderi Men, 2017. A worldwide checklist and a key to Chinese species of the subgenus Emodotipula are provided.