[Objective] This study aimed to compare the microstructure between I. in- digotica Fort. and B. cusia （Nees） Bremek. [Method] Microstructure of I. indigotica Fort. and B. cusia （Nees） Bremek. with different source...[Objective] This study aimed to compare the microstructure between I. in- digotica Fort. and B. cusia （Nees） Bremek. [Method] Microstructure of I. indigotica Fort. and B. cusia （Nees） Bremek. with different sources were compared and analyzed by using digital microscopy. [Result] Comparison result of the microstructure shows that reticulate vessels, single starch granules, multiple starch granules and stone cells are mostly observed in I. indigotica Fort.; pitted vessels, parenchyma cells with small pits, single starch granules and cystolith are mostly observed in B. cusia （Nees） Bremek., which indicated that microstructure of L indigotica Fort. and B. cusia （Nees） Bremek. are significantly different. [Conclusion] Microstructure char- acteristics of I. indigotica Fort. and B. cusia （Nees） Bremek. established in this study can be used to evaluate the quality of these two kinds of Chinese herbal medicines, which also provide scientific basis and methods for comprehensively studying the quality standard of /. indigotica Fort. and B. cusia （Nees） Bremek. Key words Isatis indigotica Fort.; Baphicacanthus cusia （Nees） Bremek.; Microstruc- ture; Quality展开更多
Protein extraction is a critical step for two-dimensional electrophoresis （2-DE）. Different plant samples require different and adaptive protein extraction protocols. The leaves of medicinal plant, Baphicacanthus cu...Protein extraction is a critical step for two-dimensional electrophoresis （2-DE）. Different plant samples require different and adaptive protein extraction protocols. The leaves of medicinal plant, Baphicacanthus cusia （Nees） Bremek are notoriously recalcitrant to common protein extraction methods due to high levels of interfering compounds （especially the secondary metabolites and pigments）. This study was aimed to establish a routine procedure for the proteomic analysis ofB. cusia leaves, and a new protocol for the protein extraction was developed by optimizing trichloroacetic acid （TCA）/ acetone extraction method. The efficiency of this protocol was demonstrated by comparison with 3 published protein extraction methods （chloroform/acetone, Mg/NP-40, Tris-base/acetone）. The results showed that the optimized TCA/ acetone precipitation extraction method gave a relatively high protein yield （9.263 mg g^-1 fresh weight）, high-resolution separation, clear protein profiles, the highest proteins spots （1 31 t protein spots）, and displayed less contamination in 2- DE gels. Therefore, the results suggested that the optimized TCA/acetone method was the most effective among the 4 methods for B. cusia leaves.展开更多
This study explored the extraction process of indigo pigment from Baphicacanthus cusia using aqueous enzymatic method for the first time. Through single factor tests and orthogonal tests, this study investigated the e...This study explored the extraction process of indigo pigment from Baphicacanthus cusia using aqueous enzymatic method for the first time. Through single factor tests and orthogonal tests, this study investigated the effects of extraction time, extraction temperature, enzyme concentration, pH value, and solidliquid ratio on the extraction rate of indigo pigment. The results showed that the extraction rate of indigo reached the highest(0.218%) when the solid-liquid ratio was 1:20, enzyme concentration was 6 g/L, pH value was 5, extraction temperature was 50℃, and extraction time was 3 h. The method had greatly shortened the extraction time, simplified the extraction process, and improved the extraction efficiency while producing little pollution and meeting the green environmental protection requirements.展开更多
文摘[Objective] This study aimed to compare the microstructure between I. in- digotica Fort. and B. cusia （Nees） Bremek. [Method] Microstructure of I. indigotica Fort. and B. cusia （Nees） Bremek. with different sources were compared and analyzed by using digital microscopy. [Result] Comparison result of the microstructure shows that reticulate vessels, single starch granules, multiple starch granules and stone cells are mostly observed in I. indigotica Fort.; pitted vessels, parenchyma cells with small pits, single starch granules and cystolith are mostly observed in B. cusia （Nees） Bremek., which indicated that microstructure of L indigotica Fort. and B. cusia （Nees） Bremek. are significantly different. [Conclusion] Microstructure char- acteristics of I. indigotica Fort. and B. cusia （Nees） Bremek. established in this study can be used to evaluate the quality of these two kinds of Chinese herbal medicines, which also provide scientific basis and methods for comprehensively studying the quality standard of /. indigotica Fort. and B. cusia （Nees） Bremek. Key words Isatis indigotica Fort.; Baphicacanthus cusia （Nees） Bremek.; Microstruc- ture; Quality
基金supported by the Department of Edu-cation(JA05238)the Key Project on Ecology of Fujian Province,China(0608537)
文摘Protein extraction is a critical step for two-dimensional electrophoresis （2-DE）. Different plant samples require different and adaptive protein extraction protocols. The leaves of medicinal plant, Baphicacanthus cusia （Nees） Bremek are notoriously recalcitrant to common protein extraction methods due to high levels of interfering compounds （especially the secondary metabolites and pigments）. This study was aimed to establish a routine procedure for the proteomic analysis ofB. cusia leaves, and a new protocol for the protein extraction was developed by optimizing trichloroacetic acid （TCA）/ acetone extraction method. The efficiency of this protocol was demonstrated by comparison with 3 published protein extraction methods （chloroform/acetone, Mg/NP-40, Tris-base/acetone）. The results showed that the optimized TCA/ acetone precipitation extraction method gave a relatively high protein yield （9.263 mg g^-1 fresh weight）, high-resolution separation, clear protein profiles, the highest proteins spots （1 31 t protein spots）, and displayed less contamination in 2- DE gels. Therefore, the results suggested that the optimized TCA/acetone method was the most effective among the 4 methods for B. cusia leaves.
文摘This study explored the extraction process of indigo pigment from Baphicacanthus cusia using aqueous enzymatic method for the first time. Through single factor tests and orthogonal tests, this study investigated the effects of extraction time, extraction temperature, enzyme concentration, pH value, and solidliquid ratio on the extraction rate of indigo pigment. The results showed that the extraction rate of indigo reached the highest(0.218%) when the solid-liquid ratio was 1:20, enzyme concentration was 6 g/L, pH value was 5, extraction temperature was 50℃, and extraction time was 3 h. The method had greatly shortened the extraction time, simplified the extraction process, and improved the extraction efficiency while producing little pollution and meeting the green environmental protection requirements.
