[Objective] The aim is to study the construction of vectors expressing foreign protein in potato starch grains specifically, and provide some reference for solving industrialized core problem of high cost and low expr...[Objective] The aim is to study the construction of vectors expressing foreign protein in potato starch grains specifically, and provide some reference for solving industrialized core problem of high cost and low expression level of foreign protein. [Method] By using molecular biological techniques of RT-PCR and nested PCR, plant expression vector for the foreign protein locating in the potato starch grains was constructed. [ Result] Coding sequence （ GC20 ） of potato starch grains that was located and expressed by GBSSI promoter was cloned. Plant expression vector was screened out through connection, transformation and enzyme digestion identification. [ Conclusion] This result laid a foundation for further screening the foreign protein on the potato starch grains.展开更多
For the integration network of a cellular network and a wireless local area network （WLAN）, a priority-based dynamic load transfer （PDLT） algorithm is proposed. The dynamic vertical handoffs by call admission cont...For the integration network of a cellular network and a wireless local area network （WLAN）, a priority-based dynamic load transfer （PDLT） algorithm is proposed. The dynamic vertical handoffs by call admission control are jointly determined by the network conditions and the traffic characteristics in combination with the location-condition of mobile terminals. When there is no bandwidth resource available in the cellular network or WLAN, the proposed PDLT algorithm allows an incoming voice call or data call within the overlapping area of the cellular network and the WLAN to be directed to the spare network; meanwhile, by dynamically computing the occupancy of the bandwidth resource, the proposed PDLT algorithm also allows an ongoing voice call or data communication to be transferred to the network with a sufficient bandwidth resource according to the given threshold to balance the number of voice/data calls in the two networks. The analysis results of a two-dimensional Markov model and the simulation results show that the PDLT algorithm can effectively enhance the whole integrated network＇ s traffic, reduce the blocking probability of new calls and increase the data throughput, and thus decrease the response time for various services.展开更多
The authors propose a two-stage method for recognizing driving situations on the basis of driving signals for application to a safe human interface of an in-vehicle information system. In first stage, an unknown drivi...The authors propose a two-stage method for recognizing driving situations on the basis of driving signals for application to a safe human interface of an in-vehicle information system. In first stage, an unknown driving situation is determined as stopping behavior or non-stopping behavior. In second stage, a Hidden Markov Model （HMM）-based pattern recognition method is used to model and recognize six non-stopping driving situations. The authors attempt to find the optimal HMM configuration to improve the performance of driving situation recognition. Center for Integrated Acoustic Information Research （CLAIR） in-vehicle corpus is used to evaluate the HMM-based recognition method. Driving situation categories are recognized using five driving signals. The proposed method achieves a relative error reduction rate of 30.9% compared to a conventional one-stage based HMMs.展开更多
We reported herein the synthesis and antibacterial activity evaluation of two oxazolidinone-deferasirox conjugates with different linkers that were designed based on the“Trojan horse”strategy.The conjugates could co...We reported herein the synthesis and antibacterial activity evaluation of two oxazolidinone-deferasirox conjugates with different linkers that were designed based on the“Trojan horse”strategy.The conjugates could combine with Fe^(3+)ions as the deferasirox.However,both conjugates were inactive against tested bacteria,including S.aureus,E.coli,A.baumannii,and P.aeruginosa.The results suggested that the synthesized iron chelator deferasirox was not suitable as a siderophore of the bacteria to transport the antibiotic,or the coupling linker of the synthesized conjugates could not be hydrolyzed to release the oxazolidinone in the cytoplasm.Therefore,the design and synthesis of oxazolidinone-deferasirox conjugates need further exploration.展开更多
Apolipoprotein C1 （Apocl） is associated with lipoprotein metabolism, but its physiological role during embryogenesis is largely unknown. We reveal a new function of Apoclb, a transcript isoform of Apocl, in epiboly ...Apolipoprotein C1 （Apocl） is associated with lipoprotein metabolism, but its physiological role during embryogenesis is largely unknown. We reveal a new function of Apoclb, a transcript isoform of Apocl, in epiboly during zebrafish gastrulation. Apoclb is expressed in yolk syncytial layers and in deep cells of the ventral and lateral region of the embryos. It displays a ra- dial gradient with high levels in the interior layer and low levels in the superficial layer. Knockdown of Apoclb by injecting antisense morpholino （MO） caused the epiboly arrest in deep cells. Moreover, we show that the radial intercalation and the ra- dial gradient distribution of E-cadherin are disrupted both in Apoclb knockdown and overexpressed embryos. Therefore, Apoc 1 b controls epiboly via E-cadherin-mediated radial intercalation in a gradient-dependent manner.展开更多
基金Supported by the National Natural Science Foundation Program(30160010)~~
文摘[Objective] The aim is to study the construction of vectors expressing foreign protein in potato starch grains specifically, and provide some reference for solving industrialized core problem of high cost and low expression level of foreign protein. [Method] By using molecular biological techniques of RT-PCR and nested PCR, plant expression vector for the foreign protein locating in the potato starch grains was constructed. [ Result] Coding sequence （ GC20 ） of potato starch grains that was located and expressed by GBSSI promoter was cloned. Plant expression vector was screened out through connection, transformation and enzyme digestion identification. [ Conclusion] This result laid a foundation for further screening the foreign protein on the potato starch grains.
基金The National Science and Technology Major Project(No. 2011ZX03005-004-03 )the National Natural Science Foundation of China (No. 61171081 )the Research Fund of the National Mobile Communications Research Laboratory of Southeast University (No.2011A08)
文摘For the integration network of a cellular network and a wireless local area network （WLAN）, a priority-based dynamic load transfer （PDLT） algorithm is proposed. The dynamic vertical handoffs by call admission control are jointly determined by the network conditions and the traffic characteristics in combination with the location-condition of mobile terminals. When there is no bandwidth resource available in the cellular network or WLAN, the proposed PDLT algorithm allows an incoming voice call or data call within the overlapping area of the cellular network and the WLAN to be directed to the spare network; meanwhile, by dynamically computing the occupancy of the bandwidth resource, the proposed PDLT algorithm also allows an ongoing voice call or data communication to be transferred to the network with a sufficient bandwidth resource according to the given threshold to balance the number of voice/data calls in the two networks. The analysis results of a two-dimensional Markov model and the simulation results show that the PDLT algorithm can effectively enhance the whole integrated network＇ s traffic, reduce the blocking probability of new calls and increase the data throughput, and thus decrease the response time for various services.
文摘The authors propose a two-stage method for recognizing driving situations on the basis of driving signals for application to a safe human interface of an in-vehicle information system. In first stage, an unknown driving situation is determined as stopping behavior or non-stopping behavior. In second stage, a Hidden Markov Model （HMM）-based pattern recognition method is used to model and recognize six non-stopping driving situations. The authors attempt to find the optimal HMM configuration to improve the performance of driving situation recognition. Center for Integrated Acoustic Information Research （CLAIR） in-vehicle corpus is used to evaluate the HMM-based recognition method. Driving situation categories are recognized using five driving signals. The proposed method achieves a relative error reduction rate of 30.9% compared to a conventional one-stage based HMMs.
基金The CAMS Innovation Fund for Medical Sciences(CIFMS 2021-I2M-1-028).
文摘We reported herein the synthesis and antibacterial activity evaluation of two oxazolidinone-deferasirox conjugates with different linkers that were designed based on the“Trojan horse”strategy.The conjugates could combine with Fe^(3+)ions as the deferasirox.However,both conjugates were inactive against tested bacteria,including S.aureus,E.coli,A.baumannii,and P.aeruginosa.The results suggested that the synthesized iron chelator deferasirox was not suitable as a siderophore of the bacteria to transport the antibiotic,or the coupling linker of the synthesized conjugates could not be hydrolyzed to release the oxazolidinone in the cytoplasm.Therefore,the design and synthesis of oxazolidinone-deferasirox conjugates need further exploration.
基金supported by grants from the National Basic Research Program of China(2010CB126300)the National High Technology Research and Development Program of China(2012AA092201-3)+1 种基金Ministry of Agriculture of China within the special Fund for Agro-scientific Research in the Public Interest(200903045)the National Natural Science Foundation of China(30971414)
文摘Apolipoprotein C1 （Apocl） is associated with lipoprotein metabolism, but its physiological role during embryogenesis is largely unknown. We reveal a new function of Apoclb, a transcript isoform of Apocl, in epiboly during zebrafish gastrulation. Apoclb is expressed in yolk syncytial layers and in deep cells of the ventral and lateral region of the embryos. It displays a ra- dial gradient with high levels in the interior layer and low levels in the superficial layer. Knockdown of Apoclb by injecting antisense morpholino （MO） caused the epiboly arrest in deep cells. Moreover, we show that the radial intercalation and the ra- dial gradient distribution of E-cadherin are disrupted both in Apoclb knockdown and overexpressed embryos. Therefore, Apoc 1 b controls epiboly via E-cadherin-mediated radial intercalation in a gradient-dependent manner.
