Based on multi-alignment of complete polyprotein amino acid sequences of genus Potyvirus,five degenerated primers were designedThey were Sprimer(5′-GGX AAY AAY AGY GGX CAZ CC-3′),pNIa(+)(5′-TNY TGG AAM CAY TGG A...Based on multi-alignment of complete polyprotein amino acid sequences of genus Potyvirus,five degenerated primers were designedThey were Sprimer(5′-GGX AAY AAY AGY GGX CAZ CC-3′),pNIa(+)(5′-TNY TGG AAM CAY TGG AT-3′),pCI2(+)(5′-GCX ACX AAX ATX ATX GAX AA-3′),pCI1(+)(5′-GTX GGX TCX GGX AAX TCX AC-3′)and pHC(+)(5′-TGY GAY AAY CAZ TTX GA-3′)(X=A,T,C or G:Y=T or C;Z=A or G;N=A or T;M=A,T or G)Using degenerated PCR and modified RACE methods,a protocol for determination of complete genome sequence of potyviruses was established and proved to be successful on five展开更多
【目的】马铃薯Y病毒属(Potyvirus)是世界上最大的两个植物病毒属之一,对多种农作物造成危害。利用文献数据库,客观地分析国内外Potyvirus研究的发展动态,为国内外Potyvirus科研工作者和决策者提供参考。【方法】基于Web of Science数据...【目的】马铃薯Y病毒属(Potyvirus)是世界上最大的两个植物病毒属之一,对多种农作物造成危害。利用文献数据库,客观地分析国内外Potyvirus研究的发展动态,为国内外Potyvirus科研工作者和决策者提供参考。【方法】基于Web of Science数据库,采用文献计量学方法,对全球发表于1985 2012年的Potyvirus文献的国家、作者、机构、载文期刊及研究内容进行了数量和质量分析。【结果】检索到全球60个国家发表的Potyvirus文献2 442篇,以及每篇文献的被引频次和每个国家、机构及作者的H指数。经分析得出:全球Potyvirus研究论文的产出量自1991年呈稳定上升趋势,文献的数量和质量在统计的国家中,以美国最好,其次是法国、西班牙、澳大利亚、英国;美国的高产活跃作者最多;Potyvirus研究的热点是基因沉默和分子生物学,并随着研究技术方法的革新将不断深入;研究方向更多倾向于寄主方面的研究(如基因沉默、寄主抗性、与寄主互作等)及交叉学科;而中国在Potyvirus基础研究上距离国际先进水平还有很大距离,研究力量较少,有影响力的论文较少。【结论】美国在该领域的研究处于领先水平,我国仍需加强Potyvirus基础研究力量投入,努力缩短差距。展开更多
YN80 was isolated from Amorphophallus rivieri Durieu showing mosaic and crinkle symptoms in Songming,Yunnan province.Flexuous filamentous particles were found in diseased leave sap and pinwheel inclusion bodies were f...YN80 was isolated from Amorphophallus rivieri Durieu showing mosaic and crinkle symptoms in Songming,Yunnan province.Flexuous filamentous particles were found in diseased leave sap and pinwheel inclusion bodies were found in the leave tissue.YN80 had positive reaction to universal antibody of Potyvirus by DAS-ELISA.3’-terminal sequence of YN80 was cloned and sequenced.cp gene of YN80 consisted of 987 nt,encoded 328 aa(36.1 kDa).Sequence analysis showed that YN80 shared the highest identity(97.0%)with CP amino acid sequence of Dasheen mosaic virus(DsMV).These data indicated that YN80 was an isolate of DsMV.This is the first molecular identification of A.rivieri Durieu isolate of DsMV in China.展开更多
文摘Based on multi-alignment of complete polyprotein amino acid sequences of genus Potyvirus,five degenerated primers were designedThey were Sprimer(5′-GGX AAY AAY AGY GGX CAZ CC-3′),pNIa(+)(5′-TNY TGG AAM CAY TGG AT-3′),pCI2(+)(5′-GCX ACX AAX ATX ATX GAX AA-3′),pCI1(+)(5′-GTX GGX TCX GGX AAX TCX AC-3′)and pHC(+)(5′-TGY GAY AAY CAZ TTX GA-3′)(X=A,T,C or G:Y=T or C;Z=A or G;N=A or T;M=A,T or G)Using degenerated PCR and modified RACE methods,a protocol for determination of complete genome sequence of potyviruses was established and proved to be successful on five
文摘【目的】马铃薯Y病毒属(Potyvirus)是世界上最大的两个植物病毒属之一,对多种农作物造成危害。利用文献数据库,客观地分析国内外Potyvirus研究的发展动态,为国内外Potyvirus科研工作者和决策者提供参考。【方法】基于Web of Science数据库,采用文献计量学方法,对全球发表于1985 2012年的Potyvirus文献的国家、作者、机构、载文期刊及研究内容进行了数量和质量分析。【结果】检索到全球60个国家发表的Potyvirus文献2 442篇,以及每篇文献的被引频次和每个国家、机构及作者的H指数。经分析得出:全球Potyvirus研究论文的产出量自1991年呈稳定上升趋势,文献的数量和质量在统计的国家中,以美国最好,其次是法国、西班牙、澳大利亚、英国;美国的高产活跃作者最多;Potyvirus研究的热点是基因沉默和分子生物学,并随着研究技术方法的革新将不断深入;研究方向更多倾向于寄主方面的研究(如基因沉默、寄主抗性、与寄主互作等)及交叉学科;而中国在Potyvirus基础研究上距离国际先进水平还有很大距离,研究力量较少,有影响力的论文较少。【结论】美国在该领域的研究处于领先水平,我国仍需加强Potyvirus基础研究力量投入,努力缩短差距。
文摘YN80 was isolated from Amorphophallus rivieri Durieu showing mosaic and crinkle symptoms in Songming,Yunnan province.Flexuous filamentous particles were found in diseased leave sap and pinwheel inclusion bodies were found in the leave tissue.YN80 had positive reaction to universal antibody of Potyvirus by DAS-ELISA.3’-terminal sequence of YN80 was cloned and sequenced.cp gene of YN80 consisted of 987 nt,encoded 328 aa(36.1 kDa).Sequence analysis showed that YN80 shared the highest identity(97.0%)with CP amino acid sequence of Dasheen mosaic virus(DsMV).These data indicated that YN80 was an isolate of DsMV.This is the first molecular identification of A.rivieri Durieu isolate of DsMV in China.
