In this report, we describe the isolation and characterization of microsatellite loci for forest musk deer （Moschus berezovskii） through an improved enrichment protocol. Five new markers were isolated from the genom...In this report, we describe the isolation and characterization of microsatellite loci for forest musk deer （Moschus berezovskii） through an improved enrichment protocol. Five new markers were isolated from the genomic DNA of forest musk deer and showed high polymorphism with 4 - 13 alleles in 24 sampled individuals from the population of Jinfeng Mountain, Sichuan Province, China. The observed and expected heterozygesities were from 0.429 - 0.957 and 0.587 - 0.902, respectively. The average polymorphism information content （PIC） value in these five loci was 0. 730. This suggests that the five microsatellite loci are a valuable tool for further studies about forest musk deer.展开更多
Anhui musk deer [ Moschus ( moschiferus/berezovskii ) anhuiensis ] has been a taxonomic mystery since its discovery in early 80's.In this paper,with museum samples,we sequenced the complete cytochrome b ge...Anhui musk deer [ Moschus ( moschiferus/berezovskii ) anhuiensis ] has been a taxonomic mystery since its discovery in early 80's.In this paper,with museum samples,we sequenced the complete cytochrome b gene of five Anhui musk deer.When compared with other species in Genus Moschus ,Anhui musk deer showed a rather level of sequence divergence from all the other species in this genus.The phylogenetic trees constructed by multiple methods supported the same topology,in which the monophyly of Anhui musk deer was clearly demonstrated.Therefore,our molecular data suggest a full species status for Anhui musk deer ( Moschus anhuiensis ),rather than a subspecies of either M moschiferus or M berezovskii previously suggested by morphological studies.展开更多
研究野生动物栖息地空间分布格局及其与环境因素的关系,对了解该物种适宜生境的空间分布以及生境需求有重要意义。开展珍稀物种原麝(Moschus moschiferus)的空间分布格局研究,可为其栖息地管理和保护提供科学依据。利用MaxEnt模型对陕...研究野生动物栖息地空间分布格局及其与环境因素的关系,对了解该物种适宜生境的空间分布以及生境需求有重要意义。开展珍稀物种原麝(Moschus moschiferus)的空间分布格局研究,可为其栖息地管理和保护提供科学依据。利用MaxEnt模型对陕西黄龙山原麝潜在生境及其主要影响因子进行定量分析,并确定原麝在该区域的潜在分布范围和关键影响因子及其阈值。结果表明,模型预测结果达到优秀水平,原麝潜在分布关键限制因子及其阈值为最干季平均温度<-3.6℃,到道路的距离>4100 m,坡度>71.5°,降水量变化方差为81.5~85.0,最适为83.5,昼夜温差月均值<11.6℃,最适为9.5℃。原麝的适宜区主要分布在宜川县的东南部、黄龙县的东北部及与洛川交界处、韩城的西北部,中等适宜区主要分布在宜川县适宜区周边及县城的西南部、黄龙县适宜区周边及西北部和东部、洛川县的东北部、韩城的西北部适宜区周边。适宜区面积为677.75 km ^(2),中等适宜区为2797.75 km ^(2)。各县域适宜区面积从大到小依次为宜川、韩城、黄龙和洛川,中等适宜区为黄龙、宜川、洛川和韩城。建议加强原麝适宜区的重点保护以及中等适宜区的保护和管理,控制进山人员的数量并进行教育,减少对原麝的干扰和捕杀。同时,建议林业部门近期把宜川县的原麝适宜区调整到陕西黄龙山国家级自然保护区,远期在洛川、黄龙和宜川的交界处成立新的保护区。展开更多
文摘In this report, we describe the isolation and characterization of microsatellite loci for forest musk deer （Moschus berezovskii） through an improved enrichment protocol. Five new markers were isolated from the genomic DNA of forest musk deer and showed high polymorphism with 4 - 13 alleles in 24 sampled individuals from the population of Jinfeng Mountain, Sichuan Province, China. The observed and expected heterozygesities were from 0.429 - 0.957 and 0.587 - 0.902, respectively. The average polymorphism information content （PIC） value in these five loci was 0. 730. This suggests that the five microsatellite loci are a valuable tool for further studies about forest musk deer.
基金This work received supportsfrom Natural Science Foundation of Yun nan Science Technology Committee granted to SuBingpartlys
文摘Anhui musk deer [ Moschus ( moschiferus/berezovskii ) anhuiensis ] has been a taxonomic mystery since its discovery in early 80's.In this paper,with museum samples,we sequenced the complete cytochrome b gene of five Anhui musk deer.When compared with other species in Genus Moschus ,Anhui musk deer showed a rather level of sequence divergence from all the other species in this genus.The phylogenetic trees constructed by multiple methods supported the same topology,in which the monophyly of Anhui musk deer was clearly demonstrated.Therefore,our molecular data suggest a full species status for Anhui musk deer ( Moschus anhuiensis ),rather than a subspecies of either M moschiferus or M berezovskii previously suggested by morphological studies.
文摘研究野生动物栖息地空间分布格局及其与环境因素的关系,对了解该物种适宜生境的空间分布以及生境需求有重要意义。开展珍稀物种原麝(Moschus moschiferus)的空间分布格局研究,可为其栖息地管理和保护提供科学依据。利用MaxEnt模型对陕西黄龙山原麝潜在生境及其主要影响因子进行定量分析,并确定原麝在该区域的潜在分布范围和关键影响因子及其阈值。结果表明,模型预测结果达到优秀水平,原麝潜在分布关键限制因子及其阈值为最干季平均温度<-3.6℃,到道路的距离>4100 m,坡度>71.5°,降水量变化方差为81.5~85.0,最适为83.5,昼夜温差月均值<11.6℃,最适为9.5℃。原麝的适宜区主要分布在宜川县的东南部、黄龙县的东北部及与洛川交界处、韩城的西北部,中等适宜区主要分布在宜川县适宜区周边及县城的西南部、黄龙县适宜区周边及西北部和东部、洛川县的东北部、韩城的西北部适宜区周边。适宜区面积为677.75 km ^(2),中等适宜区为2797.75 km ^(2)。各县域适宜区面积从大到小依次为宜川、韩城、黄龙和洛川,中等适宜区为黄龙、宜川、洛川和韩城。建议加强原麝适宜区的重点保护以及中等适宜区的保护和管理,控制进山人员的数量并进行教育,减少对原麝的干扰和捕杀。同时,建议林业部门近期把宜川县的原麝适宜区调整到陕西黄龙山国家级自然保护区,远期在洛川、黄龙和宜川的交界处成立新的保护区。
