临床医学留学生实验诊断学教学的实践与体会

为满足临床医学本科留学生实验诊断学的教学需要,探索建立相应的教学体系至关重要。总结讨论3年来留学生实验诊断学英语教学的改革与实践,充分了解教学对象,加强教师英语沟通能力的培养,编撰新的适用教材,应用多种现代化教学模式,依据各附属医院的优势学科选配教学内容,最终建立一种适合于留学生实验诊断学教学体系,以提高教学质量。

Progress in Plasma Diagnostics and Physics Experiment

The development of eight new diagnostic systems and the improvement of the laser Thomson scattering system contributed to the experimental campaign on HL-2A in 2006. Besides, the accuracy and flexibility of the molecular beam injection （MBI） system was improved greatly by using the special driver and controller, it is more convenient for studies such as particle transport. In the field of plasma physics experiment, the features of 3-D GAM zonal flows were further studied and some signs of low frequency zonal flows were observed. In the ECRH experiments, the effects of ECRH on sawtooth activities and m/n= 1/1 mode were studied under different plasma discharge conditions. The effect of MBI during ECRH on plasma behavior, such as non-local heat transport, was also studied.

Diagnostic accuracy of a rapid fecal test to confirm H pylori eradication after therapy: Prospective comparison with a laboratory stool test

AIM: To investigate the clinical performances of rapidstool test (ImmunoCard STAT HpSA, Meridian DiagnosticInc.) in the evaluation of eradication therapy of H pyloriand to compare it with a well-known and validatedlaboratory stool test (Amplified IDEA Hp StAR, Dako).METHODS: Stool samples of 122 patients wereevaluated after eradication therapy of H pylori. H pyloristatus was assessed by 13C-urea breath test (UBT).Stool specimens were tested using either the rapidimmunoassay kit or the laboratory immunoassay kit.RESULTS: Forty-three patients were infected and 79non-infected. Sensitivity and specificity of ImmunoCardSTAT and Hp StAR were 58.14% and 76.4%, and97.47% and 98.73%, respectively (P > 0.05). Overallagreement between the two tests was 92.6% (113 of122 cases).CONCLUSION: ImmunoCard STAT seems to haverather low performances, and it cannot be regarded as areliable tool in the post-treatment setting. Also Hp StARcannot be recommended to confirm H pylori eradicationafter treatment.

PRRS in Vietnam and Its Diagnosis

Genotyping 42 serum samples taken from the pigs in the oubreaks from 2009 to 2013 with RT-PCR and nested RT-PCR, showed more than 80% of samples were positive with Chinese PRRSV clade, and the others were European and North American classical PRRSV genotypes. Ten serum samples from unnapprent PRRS herds were examined for antibodies against PRRSV with ELISA and also for PRRSV with RT-PCR. It was clearly that the titer of antibodies against PRRSV by ELISA test could not be used for interpreting PRRSV infection. In despite of PRRS vaccination or non-vaccination, a risk of PRRSV infection and re-infection exist, utilizing RT-PCR in combination with serology will give the producer and veterinarian PRRSV more exact situation in the herds.

Prevalence of sensitization to weed pollens of Humulus scandens,Artemisia vulgaris,and Ambrosia artemisiifolia in northern China

Objective:Weed pollens are common sources of allergens worldwide.The prevalence of weed pollen sensitization is not yet fully known in China.The purpose of this study was to investigate the prevalence of sensitization to weed allergens from Artemisia,Ambrosia,and Humulus in northern China.Methods:A total of 1144 subjects(aged from 5 to 68 years) visiting our clinic from June to October 2011 underwent intradermal testing using a panel of 25 allergen sources.Subjects with positive skin responses to any pollen were further tested for their serum concentrations of IgE antibodies against Artemisia vulgaris,Ambrosia artemisiifolia,and Humulus scandens,and against the purified allergens,Art v 1 and Amb a 1.Results:Of 1144 subjects,170 had positive intradermal reactions to pollen and 144 donated serum for IgE testing.The prevalence of positive intradermal responses to pollens of Artemisia sieversiana,Artemisia annua,A.artemisiifolia,and H.scandens was 11.0%,10.2%,3.7%,and 6.6%,respectively.Among the intradermal positive subjects,the prevalence of specific IgE antigens to A.vulgaris was 58.3%,to A.artemisiifolia 14.7%,and to H.scandens 41.0%.The prevalence of specific IgE antigens to the allergen Art v 1 was 46.9%,and to Amb a 1 was 11.2%.The correlation between the presence of IgE antibodies specific to A.vulgaris and to the Art v 1 antigen was very high.Subjects with A.artemisiifolia specific IgE also had A.vulgaris specific IgE,but with relatively high levels of A.vulgaris IgE antibodies.There were no correlations between the presence of IgE antibodies to H.scandens and A.vulgaris or to H.scandens and A.artemisiifolia.Conclusions:The intradermal prevalence of weed pollen sensitization among allergic subjects in northern China is about 13.5%.Correlations of specific IgE antibodies suggest that pollen allergens from Artemisia and Humulus are independent sources for primary sensitization.

Protein array of Coxiella burnetii probed with Q fever sera

Coxiella burnetii is the etiological agent of Q fever.To identify its major seroreactive proteins,a subgenomic protein array was developed.A total of 101 assumed virulence-associated recombinant proteins of C.burnetii were probed with sera from mice experimentally infected with C.burnetii and sera from Q fever patients.Sixteen proteins were recognized as major seroreactive antigens by the mouse sera.Seven of these 16 proteins reacted positively with at least 45% of Q fever patient sera.Notably,HspB had the highest fluorescence intensity value and positive frequency of all the proteins on the array when probed with both Q fever patient sera and mouse sera.These results suggest that these seven major seroreactive proteins,particularly HspB,are potential serodiagnostic and subunit vaccine antigens of Q fever.

Application of radionuclide imaging to hepatic impact injury in rabbits

Objective: To investigate the role and clinical value of radionuclide imaging in hepatic impact injuries in rabbits. Methods: Rabbits were experimentally impacted on the liver with BIM IV bio impact machine. Liver imaging was performed with 99m Tc labeled sodium phytate. Liver blood pool imaging was performed with 99m Tc stannous pyrophosphate labeled red blood cells. The results of radionuclide imaging were compared with the anatomic results. Results: There was significant difference between the images of the injured liver and the control. Radio diminution and defect were shown in the injured liver areas. Various sorts of abnormal radioactivity distribution were observed with hepatic blood pool imaging. The results of the liver imaging and liver blood pool imaging were accorded with the results of the anatomic findings. Conclusions: Radionuclide imaging may well display the changes of hepatocellular structures and functions after injury, which is valuable in locating the concrete injured position and differentiating the injured degrees of liver.