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胫骨注射和左心室注射乳腺癌细胞小鼠骨定植模型的研究
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作者 黄玉凡 李晓青 《天津医科大学学报》 2024年第3期200-204,共5页
目的:探讨胫骨注射和左心室注射乳腺癌细胞构建小鼠骨定植模型的方法。方法:通过对野生型MDA-MB-231乳腺癌细胞进行慢病毒感染,构建稳定表达绿色荧光蛋白(GFP)和荧光素酶(LUC)的细胞株。将GFP+/LUC+标记的处于对数生长期的MDA-MB-231乳... 目的:探讨胫骨注射和左心室注射乳腺癌细胞构建小鼠骨定植模型的方法。方法:通过对野生型MDA-MB-231乳腺癌细胞进行慢病毒感染,构建稳定表达绿色荧光蛋白(GFP)和荧光素酶(LUC)的细胞株。将GFP+/LUC+标记的处于对数生长期的MDA-MB-231乳腺癌细胞分别以6×10^(3)个/只和1×10^(5)个/只接种BALB/c-nu/nu小鼠和NOD/SCID小鼠,构建胫骨注射模型和左心室注射模型。采用活体成像观察肿瘤细胞在活体小鼠体内的定植部位及分布情况;X线检测观察骨损伤的位置及程度;苏木素-伊红(HE)染色和广谱角蛋白(Pan CK)免疫组化染色定位骨中的肿瘤细胞。结果:经荧光显微镜及活细胞成像检测,表达GFP和LUC的慢病毒对MDA-MB-231细胞感染效率高达95%,且继续传代后GFP和LUC表达效率无明显减退,可用于后续实验。胫骨注射4 h后进行活体成像观察,结果显示小鼠右下肢胫骨出现LUC信号;左心室注射后立即进行活体成像观察,发现肿瘤细胞已循环至全身,表明已成功构建胫骨注射模型及左心室注射模型。30 d进行活体成像观察发现,胫骨注射模型和左心室注射模型腿部出现LUC信号,表明腿部有定植灶形成。X线结果也表明存在骨损伤。HE染色和Pan CK免疫组化染色证实胫骨注射MDA-MB-231乳腺癌细胞小鼠模型100%出现骨定植灶,定植率高,而左心室注射MDA-MB-231乳腺癌细胞小鼠模型仅20%出现骨定植灶。结论:运用活体成像、X线等检测技术联合病理学分析评估胫骨注射及左心室注射乳腺癌细胞骨定植小鼠模型,可提高结果分析的准确性,能为乳腺癌转移机制研究、抗转移治疗等提供实验和理论依据。 展开更多
关键词 注射 左心室注射 骨定植
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Study of treating tibial large bone defect with migration of lengthened bone segment in goats
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作者 杨柳 李起鸿 +2 位作者 周仲安 谭祖键 彭亦良 《Journal of Medical Colleges of PLA(China)》 CAS 2001年第2期115-118,共4页
Objective:To studytheeffectsof migrationof lengthenedbonesegment(MLBS)on thebloodcirculation andrepairremodelingprocessat theendsof largebonedefectof a longbone.Methods:A totalof18adultgoats wereusedand morethan35%of ... Objective:To studytheeffectsof migrationof lengthenedbonesegment(MLBS)on thebloodcirculation andrepairremodelingprocessat theendsof largebonedefectof a longbone.Methods:A totalof18adultgoats wereusedand morethan35%of the originallengthof theirlefttibiawas resected.Uppermetaphysiotomyto lengthenupperpartof thetibiawas doneandthelengthenedbonesegmentwas migratedto repairthelargebone defect.TheresultswereobservedwithX-rayfilms,Chinese-inkpermeatedtransparentsectionsandhistological study.Results:AfterMLBS,thedefectendsof thetibiaweresuppliedwithabundantbloodcirculationwhich resultedin rapidandsolidlongbonehealing.Conclusion:Repairof largebonedefectof longboneswithMLBS providesa newmethodforclinicalpractice. 展开更多
关键词 bonelengthening boneremodeling externalskeletonfixator bloodcirculation bonedefect
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A SIMPLIFIED IN VIVO DOSIMETRY FOR TOTAL BODY IRRADIATION PRIOR TO BONE MARROW TRANSPLANTATION
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作者 肖泽久 《Chinese Medical Sciences Journal》 CAS CSCD 1994年第4期242-244,共3页
For TBI (total body irradiation) prior to BMT(bone marrow transplantation )and in order to guarantee exact treatment,it is necessary to perfect in vivo dosimetry to detect any deviation of the treatment and to verify ... For TBI (total body irradiation) prior to BMT(bone marrow transplantation )and in order to guarantee exact treatment,it is necessary to perfect in vivo dosimetry to detect any deviation of the treatment and to verify the dose dis-tribution. A simplified and convenient transmission type in vivo dosimetry and problems are introduced and discussed. 展开更多
关键词 bone marrow transplantation total body irradiation in vivo dosimetry
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Treatment of tibial defect and bone nonunion with limb shortening with external fixator and reconstituted bone xenograft 被引量:1
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作者 王志刚 刘建 +5 位作者 胡蕴玉 孟国林 金格勒 袁志 王海强 戴先文 《Chinese Journal of Traumatology》 CAS 2003年第2期91-98,共8页
Objective: To explore the effect of external fixator and reconstituted bone xenograft (RBX) in the treatment of tibial bone defect, tibial bone nonunion and congenital pseudarthrosis of the tibia with limb shortening.... Objective: To explore the effect of external fixator and reconstituted bone xenograft (RBX) in the treatment of tibial bone defect, tibial bone nonunion and congenital pseudarthrosis of the tibia with limb shortening. Methods: Twenty patients ( 13 males and 7 females) with tibial bone defect, tibial bone nonunion or congenital pseudarthrosis of the tibia with limb shortening were treated with external fixation. Two kinds of external fixators were used: a half ring sulcated external fixator used in 13 patients and a combined external fixator in 7 patients. Foot-drop was corrected at the same time with external fixation in 4 patients. The shortened length of the tibia was in the range of 2-9 cm, with an average of 4.8 cm. For bone grafting, RBX was used in 12 patients, autogenous ilium was used in 3 patients and autogenous fibula was implanted as a bone plug into the medullary canal in 1 case, and no bone graft was used in 4 patients. Results: All the 20 patients were followed-up for 8 months to 7 years, averaging 51 months. Satisfactory function of the affected extremities was obtained. All the shortened extremities were lengthened to the expected length. For all the lengthening area and the fracture sites, bone union was obtained at the last. The average healing time of 12 patients treated with RBX was 4.8 months. Conclusions: Both the half ring sulcated external fixator and the combined external fixator have the advantages of small trauma, simple operation, elastic fixation without stress shielding and non-limitation from local soft tissue conditions, and there is satisfactory functional recovery of affected extremities in the treatment of tibial bone defects, tibial bone nonunion and congenital pseudarthrosis of the tibia combined with limb shortening. RBX has good biocompatibility and does not cause immunological rejections. It can also be safely used in treatment of bone nonunion and has reliable effect to promote bone healing. 展开更多
关键词 External fixators Bone transplantation Bone lengthening Bone defects
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Twenty-seven-year nonunion of a Hoffa fracture in a 46-year-old patient 被引量:4
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作者 Yirui Jiang Zhenyu Wang Debao Zhang Guishan Gu 《Chinese Journal of Traumatology》 CAS CSCD 2015年第1期54-58,共5页
A Hoffa fracture is an uncommon clinical entity typically seen in adults after high-energy trauma. Nonunion ofa Hoffa fracture appears to be even more uncommon. To our knowledge, only three cases of nonunion ofa Hoffa... A Hoffa fracture is an uncommon clinical entity typically seen in adults after high-energy trauma. Nonunion ofa Hoffa fracture appears to be even more uncommon. To our knowledge, only three cases of nonunion ofa Hoffa fracture have been documented in the literature to date, including two children and one adult. This article presents a case of an adult who had nonunion of a Hoffa fracture for 27 years and was treated by open reduction and internal fixation, and the varus deformity corrected with xenogenous bone graft. An excellent result has been achieved to date. This unusual case reminds us that we cannot neglect the possibility of nonunion of a cancellous hone fracture, especially the Hoffa fractures of the medial femoral condyle if they are treated nonoperatively. It also demonstrates that internal fixation with bone graft is effective, even for the 27-year Hoffa fracture. 展开更多
关键词 Hoffa fracture Fracture fixation internal Bone graft
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External fixation and bone grafting for collapsed and comminuted distal radius fracture
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作者 张双喜 顾方瑞 +5 位作者 彭永利 朱广明 付江 卢俊岳 王江涛 刘章民 《Chinese Journal of Traumatology》 CAS 2005年第3期156-159,164,共5页
Objective: To study the therapeutic effect of collapsed and comminuted distal radius fracture. Methods: Twenty-six patients with collapsed and comminuted distal radius fracture were hospitalized from July 1998 to June... Objective: To study the therapeutic effect of collapsed and comminuted distal radius fracture. Methods: Twenty-six patients with collapsed and comminuted distal radius fracture were hospitalized from July 1998 to June 2003. All fractures were treated by the methods of open reduction, sustained bone grafting and passing joint external fixator to restore the anatomic shape of distal radius. Results: All 26 cases were followed up, and the results showed that the fractures have been united radiographically. The joint surfaces were intact and there was no length discrepancy occurred in patient’s radius. The average volar tilt was 6° to 15° and the average ulnar tilt was 18° to 25°. According to the Dieust criterion, 19 cases were rated as excellent and 7 as good. Conclusions: The method that applying passing joint external fixator and bone grafting for the treatment of collapsed and comminuted distal radius fracture could maintain the stability of fracture and restore the length of radius and the intact of joint surface. 展开更多
关键词 Radius fracture External fixator Bone grafting
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