目的:研究补肾中药龟板对激素性骨质疏松大鼠骨量、骨微细结构、骨生物力学和骨代谢指标的改善作用,并对其分子机制进行探讨。方法:40只4月龄SD大鼠随机分为4组:空白组、模型组、阿伦磷酸钠组和龟板组。模型组、阿伦磷酸钠组及龟板组皮...目的:研究补肾中药龟板对激素性骨质疏松大鼠骨量、骨微细结构、骨生物力学和骨代谢指标的改善作用,并对其分子机制进行探讨。方法:40只4月龄SD大鼠随机分为4组:空白组、模型组、阿伦磷酸钠组和龟板组。模型组、阿伦磷酸钠组及龟板组皮下注射地塞米松造模,成功后分别用0.9%氯化钠溶液、阿伦磷酸钠和龟板进行灌胃。12周后取大鼠腰椎(L1-6)和血清进行检测:双能X线检测腰椎骨量、micro-CT检测腰椎骨微细结构、压缩实验检测腰椎生物力学,HE染色观察腰椎形态学改变,ELISA检测大鼠PINP和β-CTX水平表达,q PCR检测腰椎Runx2、SP-7、CTSK和AP-1的m RNA表达。结果:空白组、龟板组及阿伦磷酸钠组骨密度、骨矿物质含量、骨表面积、骨小梁数量、骨小梁厚度、压缩强度明显高于模型组(P<0.05,P<0.01),骨小梁间距、PINP和β-CTX水平、CTSK m RNA表达水平明显低于模型组(P<0.05,P<0.01)。结论:补肾中药龟板可能通过降低骨转换有效改善激素性骨质疏松;CTSK可能是龟板改善激素性骨质疏松大鼠骨量、骨微细结构、骨生物力学和骨代谢的作用靶点之一。展开更多
To investigate the effect of moderate hypothermia on respon ses of axonal cytoskeleton to axonal injury in the acute stage of injury. Methods: Of fifteen adult guinea pigs, twelve animals were subj ected to stretch in...To investigate the effect of moderate hypothermia on respon ses of axonal cytoskeleton to axonal injury in the acute stage of injury. Methods: Of fifteen adult guinea pigs, twelve animals were subj ected to stretch injury to the right optic nerves and divided into the normother mic group (n=6) in which the animals core temperature was maintained at 36.0 37.5℃ and the hypothermia group (n=6) in which the core temperature was red uced to 32.0 32.5℃ after stretch injury. Remaining three animals sustained no injury to the right optic nerves and served as control group. Half of injure d animals (n=3) of either normothermic group or hypothermic group were killed at either 2 hours or 4 hours after injury. The ultrastructural changes of axonal c ytoskeleton of the right optic nerve fibers from the animals were examined under a transmission electron microscope and analyzed by quantitative analysis with a computer image analysis system. Results: At 2 hours after stretch injury, there was a significa nt reduction in the mean number of microtubules (P< 0.001 ), and a significant increase in the mean intermicrotubule spacing (P< 0.05 or P< 0.01 ) in axons of all siz es in normothermic animals. The mean number of neurofilaments also decreased sta tistically (P< 0.01 ) in large and medium subgroups of a xons in the same experimental group at 2 hours. By 4 hours, the large subgroup o f axons in normothermic animals still demonstrated a significant decline in the mean number of microtubules (P< 0.01 ) and an increase i n the mean intermicrotubule spacing (P< 0.05 ), while th e medium and small subgroups of axons displayed a significant increase in the me an number of neurofilaments (P< 0.05 ) and reduction in the mean interneurofilament spacing (P< 0.05 ). On the c ontrary, either the mean number of microtubules and the mean intermicrotubule sp acing, or the mean number of neurofilaments and interneurofilament spacing in ax ons of all sizes in hypothermic stretch injured animals was not significant dif ferent from the mean values of sham operated animals. Conclusions: Posttraumatic moderate hypothermia induced immedia tely after axonal injury results in substantial protection of axonal cytoskeleto n and ameliorates axonal damage.展开更多
文摘目的:研究补肾中药龟板对激素性骨质疏松大鼠骨量、骨微细结构、骨生物力学和骨代谢指标的改善作用,并对其分子机制进行探讨。方法:40只4月龄SD大鼠随机分为4组:空白组、模型组、阿伦磷酸钠组和龟板组。模型组、阿伦磷酸钠组及龟板组皮下注射地塞米松造模,成功后分别用0.9%氯化钠溶液、阿伦磷酸钠和龟板进行灌胃。12周后取大鼠腰椎(L1-6)和血清进行检测:双能X线检测腰椎骨量、micro-CT检测腰椎骨微细结构、压缩实验检测腰椎生物力学,HE染色观察腰椎形态学改变,ELISA检测大鼠PINP和β-CTX水平表达,q PCR检测腰椎Runx2、SP-7、CTSK和AP-1的m RNA表达。结果:空白组、龟板组及阿伦磷酸钠组骨密度、骨矿物质含量、骨表面积、骨小梁数量、骨小梁厚度、压缩强度明显高于模型组(P<0.05,P<0.01),骨小梁间距、PINP和β-CTX水平、CTSK m RNA表达水平明显低于模型组(P<0.05,P<0.01)。结论:补肾中药龟板可能通过降低骨转换有效改善激素性骨质疏松;CTSK可能是龟板改善激素性骨质疏松大鼠骨量、骨微细结构、骨生物力学和骨代谢的作用靶点之一。
文摘To investigate the effect of moderate hypothermia on respon ses of axonal cytoskeleton to axonal injury in the acute stage of injury. Methods: Of fifteen adult guinea pigs, twelve animals were subj ected to stretch injury to the right optic nerves and divided into the normother mic group (n=6) in which the animals core temperature was maintained at 36.0 37.5℃ and the hypothermia group (n=6) in which the core temperature was red uced to 32.0 32.5℃ after stretch injury. Remaining three animals sustained no injury to the right optic nerves and served as control group. Half of injure d animals (n=3) of either normothermic group or hypothermic group were killed at either 2 hours or 4 hours after injury. The ultrastructural changes of axonal c ytoskeleton of the right optic nerve fibers from the animals were examined under a transmission electron microscope and analyzed by quantitative analysis with a computer image analysis system. Results: At 2 hours after stretch injury, there was a significa nt reduction in the mean number of microtubules (P< 0.001 ), and a significant increase in the mean intermicrotubule spacing (P< 0.05 or P< 0.01 ) in axons of all siz es in normothermic animals. The mean number of neurofilaments also decreased sta tistically (P< 0.01 ) in large and medium subgroups of a xons in the same experimental group at 2 hours. By 4 hours, the large subgroup o f axons in normothermic animals still demonstrated a significant decline in the mean number of microtubules (P< 0.01 ) and an increase i n the mean intermicrotubule spacing (P< 0.05 ), while th e medium and small subgroups of axons displayed a significant increase in the me an number of neurofilaments (P< 0.05 ) and reduction in the mean interneurofilament spacing (P< 0.05 ). On the c ontrary, either the mean number of microtubules and the mean intermicrotubule sp acing, or the mean number of neurofilaments and interneurofilament spacing in ax ons of all sizes in hypothermic stretch injured animals was not significant dif ferent from the mean values of sham operated animals. Conclusions: Posttraumatic moderate hypothermia induced immedia tely after axonal injury results in substantial protection of axonal cytoskeleto n and ameliorates axonal damage.
