不同细胞系在骨肉瘤动物模型中的成瘤差异性比较

目的:探讨不同细胞系在骨肉瘤动物模型中的成瘤差异性。方法:⑴对Mg-63和U-2 OS两系细胞分别行细胞划痕实验和transwell实验;⑵32只裸鼠随机分为Mg-63细胞悬液法、组织块法与U-2 OS细胞悬液法、组织块法4组,每组8只。两种细胞系分别采取细胞悬液法和组织块移植法进行股骨远端原位骨肉瘤重建,统计4组原位骨肉瘤模型成瘤率、瘤重以及肿瘤转移率等指标。结果:细胞划痕实验中,Mg-63和U-2 OS在划痕48 h后迁移距离分别为(381.2±23.6)、(591.9±35.1)μm。骨肉瘤细胞transwell实验中,Mg-63和U-2 OS 16 h后穿膜细胞数分别为24.0±2.6,81.9±3.1。骨肉瘤造模实验中,Mg-63悬液法组成瘤率、肺转移率、淋巴结转移率、4周瘤重分别为62.5%,10.4%,0,(2.1±0.1)g;Mg-63组织块法组分别为100%,76.8%,20.7%,(2.4±0.3)g;U-2 OS悬液法组分别为100%,45.8%,0,(2.4±0.6)g;U-2 OS组织块法组分别为100%,82.3%,41.1%,(2.6±0.4)g。结论:U-2 OS细胞系拥有较高的组织侵袭性和细胞增殖能力,是一种良好的骨肉瘤原位模型制作细胞系。

门特病专题知识讲座 正确认识“骨肿瘤”

5月17日在天津召开的第15届全国骨肿瘤学术会议上,来自北京积水潭医院的牛晓辉教授介绍,随着影像学、细胞分子生物学等技术的发展,对骨肿瘤的识别、诊断有了大幅度提升。骨肿瘤的关键在于早发现、早诊断、早治疗,一旦出现症状,应及时治疗,通过化疗、保肢手术、靶向治疗等进行综合治疗,近90%的患者可保肢,患者获得较好的生活质量。一。

液氮对Wistar大鼠股骨斜型骨折愈合的影响

目的:利用低温液氮冷冻处理从6周龄正常Wistar大鼠股骨中上段截取的实验骨块20 min后,快速复温取下的骨块,经过常规消毒处理复位,观察低温液氮对Wistar大鼠股骨斜型骨折愈合的影响。方法:6周龄Wistar大鼠10只随机分成两组,A组空白对照组5只,B组实验处理组。通过常规无菌手术处理两组Wistar大鼠右后肢股骨中上段,A组手术制造斜型骨折模型取出骨折标本进行生理盐水浸泡20 min处理后常规消毒后复位,B组手术制造斜型骨折模型取出骨折标本进行低温液氮冷冻处理20 min后迅速27℃无菌生理盐水复温后再常规消毒后复位。结果:实验研究发现,实验组的股骨实验标本回植到Wistar大鼠体内后,数周后观察骨质正常生长,骨折线生长完好,与空白对照组生长恢复情况经过统计学分析,无明显差别,无统计学意义(P >0.05)。结论:通过实验研究发现液氮冷冻技术对Wistar大鼠正常骨骨折愈合的正常生长无影响,可以应用到未来临床研究。

Establishment of a transplantation tumor model of human osteosarcoma in chick embryo

Objective: We established a transplantation tumor model of human osteosarcoma in chick embryo, studied its morphological and biological characteristics, and observed its dynamic process of angiogenesis induction so that a simple and practical model can be provided for studying osteosarcoma. Methods: Human osteosarcoma cells at different concentrations were inoculated in chorioallantoic membrane (CAM) of chick embryos at different embryonic ages to observe the factors affecting the survival of the transplanted osteosarcoma in chick embryo, growth characteristics of the transplantation tumor, and the morphological characteristics and biological characteristics of the transplantation tumor. Results: The transplantation tumor model of human osteosarcoma in chick embryo was successfully established. It was found that the transplantation tumor was easy to grow and it showed strong angiogenesis-inducing effects. Under the light microscope, the transplantation tumor showed a similar tissue structure to human osteosarcoma. Conclusion: It is feasible to establish a transplantation tumor model of human osteosarcoma in chick embryo. The model can be easily duplicated with a simple operation, which provides a useful animal model for studying osteosarcoma.

国内医学新闻

上海市卫生局科研处(200002)张勘报道 上海市肿瘤研究所高玉堂、钱耕荪研究员等,以乙型肝炎表面抗原(HBsAg)和AF-N7-鸟嘌呤加成物(AF-N7-Gua)等为标志物,进行大规模人群血清和尿样检测分子流行病学研究,以探讨黄曲霉毒素(AF)暴露与肝癌发生有关联的假设。 AF的致肝癌作用虽已在多种动物中得到证实,但由于缺乏对人体暴露于AF的有效检测手段。