采用体外细胞培养,结合流式细胞术和M T T方法研究汉防己碱对M G63细胞生长的作用特点。汉防己碱(0.33Lg/mL)对M G 63细胞仅有轻度抑制作用,细胞存活率保持在88%—97%。汉防己碱浓度依赖性轻度降低cDDP对MG63细胞的IC50;低浓度T et(0.33...采用体外细胞培养,结合流式细胞术和M T T方法研究汉防己碱对M G63细胞生长的作用特点。汉防己碱(0.33Lg/mL)对M G 63细胞仅有轻度抑制作用,细胞存活率保持在88%—97%。汉防己碱浓度依赖性轻度降低cDDP对MG63细胞的IC50;低浓度T et(0.33Lg/mL)明显提升cDD P对M G63/cDDP细胞I C50,但0.5和1.0Lg/mL显著降低cD DP对M G 63/cDDP细胞的I C50。汉防己碱对M G 63/cDDP细胞则既可增加细胞耐药发展(0.33Lg/mL),也可逆转肿瘤细胞对cDDP的耐药性(1.0Lg/mL)。展开更多
Objective: To establish a nude mice model of human osteosarcoma lung metastasis. Methods: The growth of human osteosarcoma cell sublines M8 and M6 was determined by MTT assay. 2 × 107 cells were injected into the...Objective: To establish a nude mice model of human osteosarcoma lung metastasis. Methods: The growth of human osteosarcoma cell sublines M8 and M6 was determined by MTT assay. 2 × 107 cells were injected into the tail vein of nude mice. Mice were sacrificed started on week 4 after injection, and lung metastases were evaluated under both mac-roscopic and microscopic observation with HE staining. Results: The growth of low-metastatic subline M6 was lower than high-metastatic sublines M8. Seventeen mice after injected M8 had occurred lung metastases while only one mice had oc-curred in M6 group. Moreover, M8 cells within metastases were arrangement disorder with variable nuclear hyperchromasia. Conclusion: A mouse model for human osteosarcoma cancer lung metastasis can be established by injection different ability of metastasis MG63 cells into tail vein.展开更多
文摘采用体外细胞培养,结合流式细胞术和M T T方法研究汉防己碱对M G63细胞生长的作用特点。汉防己碱(0.33Lg/mL)对M G 63细胞仅有轻度抑制作用,细胞存活率保持在88%—97%。汉防己碱浓度依赖性轻度降低cDDP对MG63细胞的IC50;低浓度T et(0.33Lg/mL)明显提升cDD P对M G63/cDDP细胞I C50,但0.5和1.0Lg/mL显著降低cD DP对M G 63/cDDP细胞的I C50。汉防己碱对M G 63/cDDP细胞则既可增加细胞耐药发展(0.33Lg/mL),也可逆转肿瘤细胞对cDDP的耐药性(1.0Lg/mL)。
基金Supported by a grant from the 973 National Great Foundation Research Program of China (No. 2002CB513100).
文摘Objective: To establish a nude mice model of human osteosarcoma lung metastasis. Methods: The growth of human osteosarcoma cell sublines M8 and M6 was determined by MTT assay. 2 × 107 cells were injected into the tail vein of nude mice. Mice were sacrificed started on week 4 after injection, and lung metastases were evaluated under both mac-roscopic and microscopic observation with HE staining. Results: The growth of low-metastatic subline M6 was lower than high-metastatic sublines M8. Seventeen mice after injected M8 had occurred lung metastases while only one mice had oc-curred in M6 group. Moreover, M8 cells within metastases were arrangement disorder with variable nuclear hyperchromasia. Conclusion: A mouse model for human osteosarcoma cancer lung metastasis can be established by injection different ability of metastasis MG63 cells into tail vein.
