背景:上颌后牙区由于上颌窦的存在限制了种植体的应用,骨诱导成骨技术的应用为上颌窦底的升高提供了可能和保障。虽然药理学、动物实验及部分临床研究均验证了重组人骨形成蛋白2/明胶海绵的骨诱导性,但是否存在研究的局限性、病例的自...背景:上颌后牙区由于上颌窦的存在限制了种植体的应用,骨诱导成骨技术的应用为上颌窦底的升高提供了可能和保障。虽然药理学、动物实验及部分临床研究均验证了重组人骨形成蛋白2/明胶海绵的骨诱导性,但是否存在研究的局限性、病例的自限性、结果的可靠性尚不十分清楚。目的:系统评价重组人骨形成蛋白2/明胶海绵在上颌窦底增高过程中牙槽嵴的增量疗效和安全性。方法:计算机检索PubMed(1966/2009-12)、Embase(1974/2009-12)、Cochrane Library(2009年第4期)、中国生物医学文献数据库CBM(1978/2009-12)、中国期刊全文数据库CNKI(1994/2009-12)、维普中文科技期刊数据库VIP(1989/2009-12)等数据库。主要检索词包括"rhBMP-2,ACS,autogenous bone graft;重组人骨形成蛋白2,明胶海绵,自体骨移植等"。采用自由词与主题词结合的方式检索,筛选在上颌窦底增高术中应用重组人骨形成蛋白2/明胶海绵修复的随机对照试验,应用RevMan5软件进行Meta分析。结果与结论:最终纳入3个研究,共288例患者。Meta分析结果提示,与自体骨移植组相比,1.5g/L重组人骨形成蛋白2/明胶海绵组牙槽嵴高度和宽度的增加差异有显著性意义;0.75g/L重组人骨形成蛋白2/明胶海绵组牙槽嵴高度的增加差异无显著性意义,牙槽嵴宽度的增加差异有显著性意义;重组人骨形成蛋白2/明胶海绵植入6个月后可改善种植区的骨密度,未有不良反应报道。提示1.5g/L,0.75g/L重组人骨形成蛋白2/明胶海绵及自体骨移植对骨缺损区都有明显的诱导成骨作用,1.5g/L重组人骨形成蛋白2/明胶海绵的效果优于自体骨。展开更多
Objective To explore the expression pattern and possible role of hypoxia inducible factor-1α （ HIF-1α ） in fetal vertebrae development of mouse. Methods The developmental stages of mice fetal vertebrae were obser...Objective To explore the expression pattern and possible role of hypoxia inducible factor-1α （ HIF-1α ） in fetal vertebrae development of mouse. Methods The developmental stages of mice fetal vertebrae were observed from embryonic days 13. 5 to 18. 5 （ E13. 5 to E18. 5 ） by stereoscopic and light microscopes respectively, and the expressions of HIF-1α at various times were also detected at levels of mRNA and protein by using methods of RT-PCR and Western blotting. Distribution of HIF-1α in the vertebrae was examined by immunohistochemical assay. Vascular endothelia growth factor （VEGF） mRNA and other chondro-osteoblast marker genes as type II collagen al （ Coll2al ） and osteocalcin （OCN） were detected by RT-PCR too. Results The cartilaginous spine column began to form at E13. 5, followed by the arising of the primary ossification center in vertebrae at E15. 5, then the osteogenesis expanded and extended to both sides of the vertebrae. HIF-1α mRNA began to express at E13. 5, and showed significantly higher level at E14. 5 （ P 〈 O. 05 ）, then declined to a low level. VEGF mRNA expressed coincidently with HIF-1α. While HIF-1α protein expression was observed at E14. 5 and lasted at low level till to birth. The expression pattern of Coll2al and OCN elucidated the cell evolution from chondrocyte to osteoblast. Conclusion The developmental pattern of vertebrae appears to be an endochondral osteogenesis process. Existed hypoxia microenviroment in the vertebrae may increase HIF-1α mRNA and protein contents thus activate VEGF expression, as may be related to the activation of other downstream genes of hypoxia inducible factor-1α and initiate the cascade of endochondral osteogenesis.展开更多
Objective： To observe the osteoinductive activity of demineralized bone matrix （DBM） and deprotenized bone （DPB） made from human avascular necrotic femoral head. Methods： The femoral head was cut into pieces wi...Objective： To observe the osteoinductive activity of demineralized bone matrix （DBM） and deprotenized bone （DPB） made from human avascular necrotic femoral head. Methods： The femoral head was cut into pieces with the size of 3 mm×3 mm×5 mm, which were made into DBM and DPB. These two kinds of biomaterials were cocultured with human bone mesenchymal stem cells （hBMSCs）. Monolayer cells without biomaterials were cultured as control. Proliferative activity ofhBMSCs was evaluated on days 1, 3, 5, 7 and 14. The concentration of alkaline phosphatase （ALP）, osteocalcin （OC）, and Ca^2＋ were detected on days 1, 7, 14 and 21. Results： Cells cultured in DBM showed higher proliferative activity than did in DPB and monolayer cells （F= 39.773, P〈0.01）. DBM and DPB also had osteoinductive activity. The concentrations of ALP （F=93.162, P〈0.01）, OC （F=236.852, P〈0.01）, Ca^2＋ （F=80.711, P〈0.01）of DBM group were significantly higher than that of DPB and control groups. Conclusions： In vitro, DBM and DPB made from avascular necrotic femoral head have osteoinductive activity when cocultured with hBMSCs, and the former is stronger than the latter.展开更多
文摘背景:上颌后牙区由于上颌窦的存在限制了种植体的应用,骨诱导成骨技术的应用为上颌窦底的升高提供了可能和保障。虽然药理学、动物实验及部分临床研究均验证了重组人骨形成蛋白2/明胶海绵的骨诱导性,但是否存在研究的局限性、病例的自限性、结果的可靠性尚不十分清楚。目的:系统评价重组人骨形成蛋白2/明胶海绵在上颌窦底增高过程中牙槽嵴的增量疗效和安全性。方法:计算机检索PubMed(1966/2009-12)、Embase(1974/2009-12)、Cochrane Library(2009年第4期)、中国生物医学文献数据库CBM(1978/2009-12)、中国期刊全文数据库CNKI(1994/2009-12)、维普中文科技期刊数据库VIP(1989/2009-12)等数据库。主要检索词包括"rhBMP-2,ACS,autogenous bone graft;重组人骨形成蛋白2,明胶海绵,自体骨移植等"。采用自由词与主题词结合的方式检索,筛选在上颌窦底增高术中应用重组人骨形成蛋白2/明胶海绵修复的随机对照试验,应用RevMan5软件进行Meta分析。结果与结论:最终纳入3个研究,共288例患者。Meta分析结果提示,与自体骨移植组相比,1.5g/L重组人骨形成蛋白2/明胶海绵组牙槽嵴高度和宽度的增加差异有显著性意义;0.75g/L重组人骨形成蛋白2/明胶海绵组牙槽嵴高度的增加差异无显著性意义,牙槽嵴宽度的增加差异有显著性意义;重组人骨形成蛋白2/明胶海绵植入6个月后可改善种植区的骨密度,未有不良反应报道。提示1.5g/L,0.75g/L重组人骨形成蛋白2/明胶海绵及自体骨移植对骨缺损区都有明显的诱导成骨作用,1.5g/L重组人骨形成蛋白2/明胶海绵的效果优于自体骨。
基金Supported by Anhui Provincial Natural Science Foundation (No. 070413097)the Special Funds for Major State Basic Research Project of Shanghai (No. 04DZ05606)
文摘Objective To explore the expression pattern and possible role of hypoxia inducible factor-1α （ HIF-1α ） in fetal vertebrae development of mouse. Methods The developmental stages of mice fetal vertebrae were observed from embryonic days 13. 5 to 18. 5 （ E13. 5 to E18. 5 ） by stereoscopic and light microscopes respectively, and the expressions of HIF-1α at various times were also detected at levels of mRNA and protein by using methods of RT-PCR and Western blotting. Distribution of HIF-1α in the vertebrae was examined by immunohistochemical assay. Vascular endothelia growth factor （VEGF） mRNA and other chondro-osteoblast marker genes as type II collagen al （ Coll2al ） and osteocalcin （OCN） were detected by RT-PCR too. Results The cartilaginous spine column began to form at E13. 5, followed by the arising of the primary ossification center in vertebrae at E15. 5, then the osteogenesis expanded and extended to both sides of the vertebrae. HIF-1α mRNA began to express at E13. 5, and showed significantly higher level at E14. 5 （ P 〈 O. 05 ）, then declined to a low level. VEGF mRNA expressed coincidently with HIF-1α. While HIF-1α protein expression was observed at E14. 5 and lasted at low level till to birth. The expression pattern of Coll2al and OCN elucidated the cell evolution from chondrocyte to osteoblast. Conclusion The developmental pattern of vertebrae appears to be an endochondral osteogenesis process. Existed hypoxia microenviroment in the vertebrae may increase HIF-1α mRNA and protein contents thus activate VEGF expression, as may be related to the activation of other downstream genes of hypoxia inducible factor-1α and initiate the cascade of endochondral osteogenesis.
文摘Objective： To observe the osteoinductive activity of demineralized bone matrix （DBM） and deprotenized bone （DPB） made from human avascular necrotic femoral head. Methods： The femoral head was cut into pieces with the size of 3 mm×3 mm×5 mm, which were made into DBM and DPB. These two kinds of biomaterials were cocultured with human bone mesenchymal stem cells （hBMSCs）. Monolayer cells without biomaterials were cultured as control. Proliferative activity ofhBMSCs was evaluated on days 1, 3, 5, 7 and 14. The concentration of alkaline phosphatase （ALP）, osteocalcin （OC）, and Ca^2＋ were detected on days 1, 7, 14 and 21. Results： Cells cultured in DBM showed higher proliferative activity than did in DPB and monolayer cells （F= 39.773, P〈0.01）. DBM and DPB also had osteoinductive activity. The concentrations of ALP （F=93.162, P〈0.01）, OC （F=236.852, P〈0.01）, Ca^2＋ （F=80.711, P〈0.01）of DBM group were significantly higher than that of DPB and control groups. Conclusions： In vitro, DBM and DPB made from avascular necrotic femoral head have osteoinductive activity when cocultured with hBMSCs, and the former is stronger than the latter.
