Objective To observe the effects of Zhuang Gu Zhi Tong Formula (ZGZTF) on antagonist SOST in canonical Wnt/β-catenin signaling pathway in osteoporosis. Methods We analyzed the differential genes of patients with oste...Objective To observe the effects of Zhuang Gu Zhi Tong Formula (ZGZTF) on antagonist SOST in canonical Wnt/β-catenin signaling pathway in osteoporosis. Methods We analyzed the differential genes of patients with osteoporosis and normal subjects from the GEO database and then found SOST with specific expression. We analyzed SOST as an antagonist of the Wnt signaling pathway by the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. Then we studied the effect of ZGZTF on SOST in Wnt signaling pathway. Osteoporosis model was induced by ovariectomy (OVX) in 8-week-old female Sprague–Dawley (SD) rats. After 12 weeks of treatment with ZGZTF by intragastric administration, the rats were put to death in batch. The changes of alkaline phosphatase (ALP), bone gla protein (BGP) and estradiol (E2) in serum were determined, and bone mineral density (BMD) and histomorphology of right femur were observed. Biomechanics of lumbar vertebra were measured, and the expression of SOST, Wnt3a,β-catenin, LRP5, Runx2, Osx and their mRNA involving the canonical Wnt/β-catenin signaling pathway were detected by Western blot, RT-PCR and Immunohistochemical analysis. All data were analyzed by SPSS 22.0. Results Twelve weeks of treatment with ZGZTF could significantly decrease the level of ALP and BGP in serum, increase the BMD of femurs, and improve the biomechanical capability of vertebral body in maximum loading and elastic modulus. Concerning histomorphology, we found ordered arrangement of trabeculae, slightly thinning of trabeculae and none obvious slight fractures in femurs after 12 weeks of treatment with ZGZTF. The expression of LRP5,β-catenin, Runx2 and Osx involved in the canonical Wnt/β-catenin signaling pathway was significantly up-regulated in the presence of ZGZTF,and the expression of SOST in this pathway was down-regulated. Conclusions These results suggest that ZGZTF may be antiosteoporosis by down-regulating SOST protein to promote Wnt/β-catenin signaling pathway.展开更多
Objective：To observe the clinical efficacy of Yi Jin Jing （Sinew-transforming Qigong Exercises） for primary osteoporosis in the elderly.Methods：Eighty old patients with primary osteoporosis were randomized into a ...Objective：To observe the clinical efficacy of Yi Jin Jing （Sinew-transforming Qigong Exercises） for primary osteoporosis in the elderly.Methods：Eighty old patients with primary osteoporosis were randomized into a Yi Jin Jing group and a medication group,40 cases in each group.The Yi Jin Jing group was intervened by Yi Jin Jing （Sinew-transforming Qigong Exercises） training,while the medication group was intervened by oral administration of alendronate sodium tablet.Prior to and after 6-month intervention,the bone mineral density （BMD） of the proximal femur,visual analog scale （VAS） and activities of daily living （ADL） were estimated.Results：There were no significant differences in the BMD of the proximal femur,and VAS and ADL scores between the two groups before the intervention （P〉0.05）.After 6-month intervention,the above items all improved significantly in both groups （both P〈0.01）;the improvements in VAS and ADL scores in Yi Jin Jing group were more significant than those in the medication group （P〈0.01）,while the between-group difference in the BMD of the proximal femur was statistically insignificant （P〉0.05）.Conclusion：Practice of Yi Jin Jing （Sinew-transforming Qigong Exercises） can effectively ameliorate the BMD in the elderly with primary osteoporosis,and it can reduce the pain and improve ADL,with a better general effect compared to oral administration of alendronate sodium tablet.展开更多
基金the funding support from the National Natural Science Foundation of China (No. 81573956)Natural Science Foundation of Hunan Province (No. 2018JJ2297)+3 种基金Key Program of Chinese Medicine Science Research Plan of Hunan Province (No. 201612)Key Program of Scientific Research Fund of Hunan Provincial Education Department (No. 16A162)National College Students Innovation and Entrepreneurship Project (No. 201710541002)The Project of Research Learning and Innovative Experiment for College Students in Hunan (No. 2015217, No. 2015220, No. 2016284 and No. 2016281)
文摘Objective To observe the effects of Zhuang Gu Zhi Tong Formula (ZGZTF) on antagonist SOST in canonical Wnt/β-catenin signaling pathway in osteoporosis. Methods We analyzed the differential genes of patients with osteoporosis and normal subjects from the GEO database and then found SOST with specific expression. We analyzed SOST as an antagonist of the Wnt signaling pathway by the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. Then we studied the effect of ZGZTF on SOST in Wnt signaling pathway. Osteoporosis model was induced by ovariectomy (OVX) in 8-week-old female Sprague–Dawley (SD) rats. After 12 weeks of treatment with ZGZTF by intragastric administration, the rats were put to death in batch. The changes of alkaline phosphatase (ALP), bone gla protein (BGP) and estradiol (E2) in serum were determined, and bone mineral density (BMD) and histomorphology of right femur were observed. Biomechanics of lumbar vertebra were measured, and the expression of SOST, Wnt3a,β-catenin, LRP5, Runx2, Osx and their mRNA involving the canonical Wnt/β-catenin signaling pathway were detected by Western blot, RT-PCR and Immunohistochemical analysis. All data were analyzed by SPSS 22.0. Results Twelve weeks of treatment with ZGZTF could significantly decrease the level of ALP and BGP in serum, increase the BMD of femurs, and improve the biomechanical capability of vertebral body in maximum loading and elastic modulus. Concerning histomorphology, we found ordered arrangement of trabeculae, slightly thinning of trabeculae and none obvious slight fractures in femurs after 12 weeks of treatment with ZGZTF. The expression of LRP5,β-catenin, Runx2 and Osx involved in the canonical Wnt/β-catenin signaling pathway was significantly up-regulated in the presence of ZGZTF,and the expression of SOST in this pathway was down-regulated. Conclusions These results suggest that ZGZTF may be antiosteoporosis by down-regulating SOST protein to promote Wnt/β-catenin signaling pathway.
文摘Objective：To observe the clinical efficacy of Yi Jin Jing （Sinew-transforming Qigong Exercises） for primary osteoporosis in the elderly.Methods：Eighty old patients with primary osteoporosis were randomized into a Yi Jin Jing group and a medication group,40 cases in each group.The Yi Jin Jing group was intervened by Yi Jin Jing （Sinew-transforming Qigong Exercises） training,while the medication group was intervened by oral administration of alendronate sodium tablet.Prior to and after 6-month intervention,the bone mineral density （BMD） of the proximal femur,visual analog scale （VAS） and activities of daily living （ADL） were estimated.Results：There were no significant differences in the BMD of the proximal femur,and VAS and ADL scores between the two groups before the intervention （P〉0.05）.After 6-month intervention,the above items all improved significantly in both groups （both P〈0.01）;the improvements in VAS and ADL scores in Yi Jin Jing group were more significant than those in the medication group （P〈0.01）,while the between-group difference in the BMD of the proximal femur was statistically insignificant （P〉0.05）.Conclusion：Practice of Yi Jin Jing （Sinew-transforming Qigong Exercises） can effectively ameliorate the BMD in the elderly with primary osteoporosis,and it can reduce the pain and improve ADL,with a better general effect compared to oral administration of alendronate sodium tablet.
