Objective To explore the expression change of stem cell-derived neural stem/progenitor cell supporting factor （SDNSF） gene in the injuried spinal cord tissues of rats, and the relation between the expressions of SDN...Objective To explore the expression change of stem cell-derived neural stem/progenitor cell supporting factor （SDNSF） gene in the injuried spinal cord tissues of rats, and the relation between the expressions of SDNSF and nestin. Methods The spinal cord contusion model of rat was established according to Allen＇s falling strike method. The expression of SDNSF was studied by RT-PCR and in situ hybridization （ISH）, and the expression of nestin was detected by immunochemistry. Results RT-PCR revealed that SDNSF mRNA was upregulated on day 4 after injury, peaked on day 8-12, and decreased to the sham operation level on day 16. ISH revealed that SDNSF mRNA was mainly expressed in the gray matter cells, probably neurons, of spinal cord. The immunohistochemistry showed that accompanied with SDNSF mRNA upregulation, the nestin-positive cells showed erupted roots, migrated peripherad and proliferation on the 8-day slice. However, the distribution pattern of these new cells was different from that of SDNSF-positive cells. Conclusion （1） SDNSF is expressed in the gray matter of spinal cord. The expression of SDNSF mRNA in the spinal cord varies with injured time. （2） The nestin-positive cells proliferate accompanied with spinal cord injury repair, but do not secrete SDNSF.展开更多
The species of the genus Eudesme from the China were are studied. There are four species in China, Eudesme virescens (Carm.) J.Ag., Eudesme huanghaiensis Ding et Lu, E.qingdaoensis Ding et Lu and E.shandongensis Ding ...The species of the genus Eudesme from the China were are studied. There are four species in China, Eudesme virescens (Carm.) J.Ag., Eudesme huanghaiensis Ding et Lu, E.qingdaoensis Ding et Lu and E.shandongensis Ding et Lu. Their morphological features and geographical distributions are discussed, a key to the species from China is provided. E.virescens is characterized by frond being 10～20 cm in height or more, 2～5 mm in diameter, solid in center, its subcortical cells being cylindrical or ellipsoidal. E.huanghaiensis is characterized by frond being 5～20 cm in height, 1.5～3.0 mm in diameter, solid, spherical subcortical cells and rhizoidal filaments developed from the basal cells of subcortext. E.qingdaoensis is characterized by frond being 10 cm in height, hollow in center, 2.5 mm in diameter, 90～230 μm long medullary cells, thin medullary wall, long and slender terminal cell of rhizoidal filaments. E.shandongensis is characterized by frond being 18～25 cm in height, hollow in center, 1.0～1.5 mm in diameter, 80～195 μm long medullary cells, slightly thick medullary wall,spherical terminal cell of rhizoidal filaments. All specimens studied are preserved in the Herbarium of the Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.展开更多
Fracture of femoral shaft in adults is common and mostly managed with intramedullary interlocking nails. Complications during closed intramedullary femoral nailing are uncommon, and mostly of them are caused by techni...Fracture of femoral shaft in adults is common and mostly managed with intramedullary interlocking nails. Complications during closed intramedullary femoral nailing are uncommon, and mostly of them are caused by technical reasons. We describe a case of closed nailing for a femoral shaft fracture in which a jammed intramedullary guide wire, due to an incarcerated bone fragment at the nail tip, was inadvertently advanced across the knee. Forceful attempt of nail insertion caused this complication, which was probably attributed to nail design.展开更多
文摘Objective To explore the expression change of stem cell-derived neural stem/progenitor cell supporting factor （SDNSF） gene in the injuried spinal cord tissues of rats, and the relation between the expressions of SDNSF and nestin. Methods The spinal cord contusion model of rat was established according to Allen＇s falling strike method. The expression of SDNSF was studied by RT-PCR and in situ hybridization （ISH）, and the expression of nestin was detected by immunochemistry. Results RT-PCR revealed that SDNSF mRNA was upregulated on day 4 after injury, peaked on day 8-12, and decreased to the sham operation level on day 16. ISH revealed that SDNSF mRNA was mainly expressed in the gray matter cells, probably neurons, of spinal cord. The immunohistochemistry showed that accompanied with SDNSF mRNA upregulation, the nestin-positive cells showed erupted roots, migrated peripherad and proliferation on the 8-day slice. However, the distribution pattern of these new cells was different from that of SDNSF-positive cells. Conclusion （1） SDNSF is expressed in the gray matter of spinal cord. The expression of SDNSF mRNA in the spinal cord varies with injured time. （2） The nestin-positive cells proliferate accompanied with spinal cord injury repair, but do not secrete SDNSF.
文摘The species of the genus Eudesme from the China were are studied. There are four species in China, Eudesme virescens (Carm.) J.Ag., Eudesme huanghaiensis Ding et Lu, E.qingdaoensis Ding et Lu and E.shandongensis Ding et Lu. Their morphological features and geographical distributions are discussed, a key to the species from China is provided. E.virescens is characterized by frond being 10～20 cm in height or more, 2～5 mm in diameter, solid in center, its subcortical cells being cylindrical or ellipsoidal. E.huanghaiensis is characterized by frond being 5～20 cm in height, 1.5～3.0 mm in diameter, solid, spherical subcortical cells and rhizoidal filaments developed from the basal cells of subcortext. E.qingdaoensis is characterized by frond being 10 cm in height, hollow in center, 2.5 mm in diameter, 90～230 μm long medullary cells, thin medullary wall, long and slender terminal cell of rhizoidal filaments. E.shandongensis is characterized by frond being 18～25 cm in height, hollow in center, 1.0～1.5 mm in diameter, 80～195 μm long medullary cells, slightly thick medullary wall,spherical terminal cell of rhizoidal filaments. All specimens studied are preserved in the Herbarium of the Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.
文摘Fracture of femoral shaft in adults is common and mostly managed with intramedullary interlocking nails. Complications during closed intramedullary femoral nailing are uncommon, and mostly of them are caused by technical reasons. We describe a case of closed nailing for a femoral shaft fracture in which a jammed intramedullary guide wire, due to an incarcerated bone fragment at the nail tip, was inadvertently advanced across the knee. Forceful attempt of nail insertion caused this complication, which was probably attributed to nail design.
