目的观察益气活血补肾方髓复康对大鼠脑缺血损伤区勿动蛋白(Nogo)-A及其受体(NgR)表达的抑制作用。方法除正常组外,采用Koizumi法制作SD大鼠单侧大脑中动脉阻塞模型(MACO),并随机分为模型组、阳性对照组及髓复康大、中、小剂量组,各组...目的观察益气活血补肾方髓复康对大鼠脑缺血损伤区勿动蛋白(Nogo)-A及其受体(NgR)表达的抑制作用。方法除正常组外,采用Koizumi法制作SD大鼠单侧大脑中动脉阻塞模型(MACO),并随机分为模型组、阳性对照组及髓复康大、中、小剂量组,各组在给药8、15、30 d 3个时间点取大鼠脑组织,采用免疫组化方法检测各组脑缺血损伤区Nogo-A及NgR表达量的差异。结果髓复康大、中、小剂量组Nogo-A、NgR表达减弱,与模型组比较差异有统计学意义(P<0.05,P<0.01)。结论髓复康可抑制脑缺血损伤区Nogo-A、NgR表达,改善脑缺血损伤区轴突再生微环境,这可能是其促进脑缺血损伤后轴突再生的机制之一。展开更多
目的研究益气活血补肾方髓复康对大鼠脑缺血损伤区勿动蛋白(NoGo-A)表达的抑制作用。方法通过Koizumi法制作SD大鼠单侧大脑中动脉阻塞模型(MACO),将SD大鼠随机分为正常组、模型组、阳性对照组、髓复康大剂量组、中剂量组和小剂量组,各...目的研究益气活血补肾方髓复康对大鼠脑缺血损伤区勿动蛋白(NoGo-A)表达的抑制作用。方法通过Koizumi法制作SD大鼠单侧大脑中动脉阻塞模型(MACO),将SD大鼠随机分为正常组、模型组、阳性对照组、髓复康大剂量组、中剂量组和小剂量组,各组又分别在给药8、15和30 d 3个时间点取大鼠脑组织,通过免疫组化、RT-PCR的方法检测各组脑缺血损伤区勿动蛋白表达量的差异。结果中药大剂量及中剂量组勿动蛋白表达最弱,均与模型组形成极显著差异(P<0.001),小剂量组的勿动蛋白表达也低于模型组,与之形成显著差异(P<0.05)。结论髓复康可以抑制脑缺血损伤区勿动蛋白的表达,改善脑缺血损伤区轴突再生微环境,可能是其促进脑缺血损伤后轴突再生的机制之一。展开更多
目的研究益气活血补肾方"髓复康"对大鼠脑缺血损伤区和体外培养神经胶质瘢痕中硫酸软骨素蛋白多糖(CSPGs)表达的抑制作用。方法通过Koizumi法制作SD大鼠单侧大脑中动脉阻塞模型(MACO),将SD大鼠随机分为正常组、模型组、阳性...目的研究益气活血补肾方"髓复康"对大鼠脑缺血损伤区和体外培养神经胶质瘢痕中硫酸软骨素蛋白多糖(CSPGs)表达的抑制作用。方法通过Koizumi法制作SD大鼠单侧大脑中动脉阻塞模型(MACO),将SD大鼠随机分为正常组、模型组、阳性对照组(激素组)、髓复康大剂量组、中剂量组和小剂量组,均在8,15和30 d 3个时间点取大鼠脑组织制作病理切片,通过免疫组化染色方法检测各组脑缺血损伤区硫酸软骨素蛋白多糖表达量的差异;建立体外培养神经胶质瘢痕模型,以血清药理学的方法选择不同浓度的含药血清,使用免疫组化染色方法分别在培养12,24,48 h后定量检测神经胶质细胞硫酸软骨素蛋白多糖的表达量。结果整体动物实验和体外培养的神经胶质细胞中,中药大剂量及中剂量组神经胶质细胞的反应性增生较轻,硫酸软骨素蛋白多糖表达最弱,均与模型组形成极显著差异(P<0.01),小剂量组的硫酸软骨素蛋白多糖表达也低于模型组,与之形成显著差异(P<0.05)。结论 "髓复康"可以抑制脑缺血损伤区硫酸软骨素蛋白多糖的表达,改善脑缺血损伤区轴突再生微环境,可能是其促进脑缺血损伤后轴突再生的机制之一。展开更多
Objective： To investigate the effect of autocontrol micromotion locking nail （AMLN） on experimental fracture healing and its mechanism. Methods： 16 goats undergoing both sides of transverse osteotomy of the femor...Objective： To investigate the effect of autocontrol micromotion locking nail （AMLN） on experimental fracture healing and its mechanism. Methods： 16 goats undergoing both sides of transverse osteotomy of the femoral shafts were fixed intramedullary with AMLN and Gross-Kempf （GK） nail, respectively. The follow-up time was 7, 14, 28 and 56 days. Rocntgenographic, biomechanieal, histological, scanning electromicroscopic and biochemical analyses were done. Results. （1） The strength of anticompression, antiflexion and antitorsion in the fractural end in the AMLN-fixed group was higher than that of GK nail-fixed group; whereas, the rate of stress shelter in the fractured end decreased significantly （ P〈0.01 ）. （ 2 ） The content of the total collagen, insoluble collagen, calcium and phosphate in the AMLN-fixed group was higher than that in the GK nail-fixed group （ P〈0.05 ）. （ 3 ） Histological observation and quantitative analysis of calluses revealed that AMLN could promote the growth of bridge calluses and periosteum calluses. Hence the facture healing and remolding process achieved early, which was much better than traditional GK nail fixation. （P〈0.05）. （4） 7-14 days postoperation, the calluses of AMLN-fixed group was flourish and camellarly arranged and the collagen fibril formed constantly in the absorption lacuna of bone trabecula. 28-56 days postoperation, the collagen fibril was flourish around the absorption lacuna and was parallel to the bone＇s longitudinal axis. Active bony absorption and formation were seen, so was remolding and rebuilding. Haversian system was intact and the bony structural net was very tenacious because of the deposition of calcium salt. None of the above findings was observed in the GK nailfixed group. Conclusions： The design of AMLN accords well with the plastic fixation theory. As the geometry ametabolic system constituted by the intramedullary fixation instruments and the proximal and distal end of the fracture is very firm and stable, the disturbance to the physical stress distributed in the fractural end is light. The generation and conduct of the intermittent physical stress between the fractural parts could reach the balance between stress conduct and stress protection. The feature that the healing and remolding take place at the same time speeds up the fractural healing process.展开更多
Objective： To observe the clinical effect of mind-refreshing and orifice-opening needling method plus swallowing disorder therapeutic apparatus for deglutition disorder of stroke patients in convalescence stage. Meth...Objective： To observe the clinical effect of mind-refreshing and orifice-opening needling method plus swallowing disorder therapeutic apparatus for deglutition disorder of stroke patients in convalescence stage. Methods： A total of 136 patients conforming to the inclusion criteria were randomized into three groups by the random number table, including group A of 46 cases, group B of 44 cases and group C of 46 cases. Patients in group A received swallowing disorder therapeutic apparatus treatment, patients in group B received mind-refreshing and orifice-opening needling method treatment, and patients in group C received mind-refreshing and orifice-opening needling method plus swallowing disorder therapeutic apparatus treatment. The treatment was given once a day for 10 d as a course, the whole treatment lasted for 4 courses. Therapeutic evaluation items including water-swallowing test （WST）, standardized swallowing assessment （SSA） and modified Barthel index （MBI） were measured before treatment, after treatment and at follow-up visit （2 months after treatment）. Results： After treatment, scores of WST and MBI in all three groups increased significantly （all P〈0.05）, while the SSA score dropped significantly （all P〈0.05）. After treatment and during follow-up visit period, score of WST in group C was significantly higher than that in group A and group B （both P〈0.05）, while the difference between group A and group B showed no statistical significance （P〉0.05）; the SSA score in group C was substantially lower than that in group A and group B （all P〈0.05）, the difference between group A and group B showed no statistical significance （P〉0.05）; the MBI scores in group B and group C were substantially higher than that in group A （all P〈0.05）, the difference between group B and group C showed no statistical significance （P〉0.05）. After treatment and during follow-up visit period, the differences in overall therapeutic effect between group A and group B showed no statistical significance （P〉0.05）, while the overall therapeutic effect in group C was substantially better than that in group A and group B （all P〈0.05）. Conclusion： Both mind-refreshing and orifice-opening needling method and swallowing disorder therapeutic apparatus can alleviate symptoms of deglutition disorder of stroke patients respectively, and the combination of two methods can improve the therapeutic effect.展开更多
Objective: To observe the clinical efficacy of Gao's nape acupuncture plus swallowing training in treating pharyngeal deglutition disorder after stroke. Methods: One hundred patients with post-stroke pharyngeal de...Objective: To observe the clinical efficacy of Gao's nape acupuncture plus swallowing training in treating pharyngeal deglutition disorder after stroke. Methods: One hundred patients with post-stroke pharyngeal deglutition disorder were randomized into a treatment group and a control group, with 50 cases in each group. The two groups both received routine neurological intervention. In addition, the treatment group was given Gao's nape acupuncture plus swallowing training, while the control group was intervened by swallowing training alone. After eight-week treatment, the two groups were observed in terms of the changes in repetitive saliva swallowing test (RSST), modified water swallowing test (MWST), standardized swallowing assessment (SSA) and swallowing-related quality of life (SWAL-QOL). The clinical efficacies of the two groups were also compared. Results: After treatment, the RSST grading, and scores of MWST, SSA and SWAL-QOL changed significantly in both groups (P<0.05 or P<0.01). The RSST grading, and scores of MWSX SSA and SWAL-QOL in the treatment group were significantly different from those in the control group after treatment (P<0.05 or P<0.01). The total effective rate and markedly effective rate were respectively 100.0% and 72.3% in the treatment group, versus 97.9% and 34.0% in the control group. There was a significant differenee in the markedly effective rate between the two groups (P<0.01). The differenee in the clinical efficacy between the two groups was statistically significant (P<0.01). Con elusion: Gao's n ape acupu ncture plus swallowi ng training is an effective approach for post-stroke phary ngeal deglutiti on disorder. Its therapeutic efficacy is more significant than that of swallowing training alone.展开更多
文摘目的观察益气活血补肾方髓复康对大鼠脑缺血损伤区勿动蛋白(Nogo)-A及其受体(NgR)表达的抑制作用。方法除正常组外,采用Koizumi法制作SD大鼠单侧大脑中动脉阻塞模型(MACO),并随机分为模型组、阳性对照组及髓复康大、中、小剂量组,各组在给药8、15、30 d 3个时间点取大鼠脑组织,采用免疫组化方法检测各组脑缺血损伤区Nogo-A及NgR表达量的差异。结果髓复康大、中、小剂量组Nogo-A、NgR表达减弱,与模型组比较差异有统计学意义(P<0.05,P<0.01)。结论髓复康可抑制脑缺血损伤区Nogo-A、NgR表达,改善脑缺血损伤区轴突再生微环境,这可能是其促进脑缺血损伤后轴突再生的机制之一。
文摘目的研究益气活血补肾方髓复康对大鼠脑缺血损伤区勿动蛋白(NoGo-A)表达的抑制作用。方法通过Koizumi法制作SD大鼠单侧大脑中动脉阻塞模型(MACO),将SD大鼠随机分为正常组、模型组、阳性对照组、髓复康大剂量组、中剂量组和小剂量组,各组又分别在给药8、15和30 d 3个时间点取大鼠脑组织,通过免疫组化、RT-PCR的方法检测各组脑缺血损伤区勿动蛋白表达量的差异。结果中药大剂量及中剂量组勿动蛋白表达最弱,均与模型组形成极显著差异(P<0.001),小剂量组的勿动蛋白表达也低于模型组,与之形成显著差异(P<0.05)。结论髓复康可以抑制脑缺血损伤区勿动蛋白的表达,改善脑缺血损伤区轴突再生微环境,可能是其促进脑缺血损伤后轴突再生的机制之一。
文摘目的研究益气活血补肾方"髓复康"对大鼠脑缺血损伤区和体外培养神经胶质瘢痕中硫酸软骨素蛋白多糖(CSPGs)表达的抑制作用。方法通过Koizumi法制作SD大鼠单侧大脑中动脉阻塞模型(MACO),将SD大鼠随机分为正常组、模型组、阳性对照组(激素组)、髓复康大剂量组、中剂量组和小剂量组,均在8,15和30 d 3个时间点取大鼠脑组织制作病理切片,通过免疫组化染色方法检测各组脑缺血损伤区硫酸软骨素蛋白多糖表达量的差异;建立体外培养神经胶质瘢痕模型,以血清药理学的方法选择不同浓度的含药血清,使用免疫组化染色方法分别在培养12,24,48 h后定量检测神经胶质细胞硫酸软骨素蛋白多糖的表达量。结果整体动物实验和体外培养的神经胶质细胞中,中药大剂量及中剂量组神经胶质细胞的反应性增生较轻,硫酸软骨素蛋白多糖表达最弱,均与模型组形成极显著差异(P<0.01),小剂量组的硫酸软骨素蛋白多糖表达也低于模型组,与之形成显著差异(P<0.05)。结论 "髓复康"可以抑制脑缺血损伤区硫酸软骨素蛋白多糖的表达,改善脑缺血损伤区轴突再生微环境,可能是其促进脑缺血损伤后轴突再生的机制之一。
基金Funded by the National Science Foundation of China ( No. 30200063 ) the Innovative Talents Project of Henan Province (No. 200084-14).
文摘Objective： To investigate the effect of autocontrol micromotion locking nail （AMLN） on experimental fracture healing and its mechanism. Methods： 16 goats undergoing both sides of transverse osteotomy of the femoral shafts were fixed intramedullary with AMLN and Gross-Kempf （GK） nail, respectively. The follow-up time was 7, 14, 28 and 56 days. Rocntgenographic, biomechanieal, histological, scanning electromicroscopic and biochemical analyses were done. Results. （1） The strength of anticompression, antiflexion and antitorsion in the fractural end in the AMLN-fixed group was higher than that of GK nail-fixed group; whereas, the rate of stress shelter in the fractured end decreased significantly （ P〈0.01 ）. （ 2 ） The content of the total collagen, insoluble collagen, calcium and phosphate in the AMLN-fixed group was higher than that in the GK nail-fixed group （ P〈0.05 ）. （ 3 ） Histological observation and quantitative analysis of calluses revealed that AMLN could promote the growth of bridge calluses and periosteum calluses. Hence the facture healing and remolding process achieved early, which was much better than traditional GK nail fixation. （P〈0.05）. （4） 7-14 days postoperation, the calluses of AMLN-fixed group was flourish and camellarly arranged and the collagen fibril formed constantly in the absorption lacuna of bone trabecula. 28-56 days postoperation, the collagen fibril was flourish around the absorption lacuna and was parallel to the bone＇s longitudinal axis. Active bony absorption and formation were seen, so was remolding and rebuilding. Haversian system was intact and the bony structural net was very tenacious because of the deposition of calcium salt. None of the above findings was observed in the GK nailfixed group. Conclusions： The design of AMLN accords well with the plastic fixation theory. As the geometry ametabolic system constituted by the intramedullary fixation instruments and the proximal and distal end of the fracture is very firm and stable, the disturbance to the physical stress distributed in the fractural end is light. The generation and conduct of the intermittent physical stress between the fractural parts could reach the balance between stress conduct and stress protection. The feature that the healing and remolding take place at the same time speeds up the fractural healing process.
文摘Objective： To observe the clinical effect of mind-refreshing and orifice-opening needling method plus swallowing disorder therapeutic apparatus for deglutition disorder of stroke patients in convalescence stage. Methods： A total of 136 patients conforming to the inclusion criteria were randomized into three groups by the random number table, including group A of 46 cases, group B of 44 cases and group C of 46 cases. Patients in group A received swallowing disorder therapeutic apparatus treatment, patients in group B received mind-refreshing and orifice-opening needling method treatment, and patients in group C received mind-refreshing and orifice-opening needling method plus swallowing disorder therapeutic apparatus treatment. The treatment was given once a day for 10 d as a course, the whole treatment lasted for 4 courses. Therapeutic evaluation items including water-swallowing test （WST）, standardized swallowing assessment （SSA） and modified Barthel index （MBI） were measured before treatment, after treatment and at follow-up visit （2 months after treatment）. Results： After treatment, scores of WST and MBI in all three groups increased significantly （all P〈0.05）, while the SSA score dropped significantly （all P〈0.05）. After treatment and during follow-up visit period, score of WST in group C was significantly higher than that in group A and group B （both P〈0.05）, while the difference between group A and group B showed no statistical significance （P〉0.05）; the SSA score in group C was substantially lower than that in group A and group B （all P〈0.05）, the difference between group A and group B showed no statistical significance （P〉0.05）; the MBI scores in group B and group C were substantially higher than that in group A （all P〈0.05）, the difference between group B and group C showed no statistical significance （P〉0.05）. After treatment and during follow-up visit period, the differences in overall therapeutic effect between group A and group B showed no statistical significance （P〉0.05）, while the overall therapeutic effect in group C was substantially better than that in group A and group B （all P〈0.05）. Conclusion： Both mind-refreshing and orifice-opening needling method and swallowing disorder therapeutic apparatus can alleviate symptoms of deglutition disorder of stroke patients respectively, and the combination of two methods can improve the therapeutic effect.
文摘Objective: To observe the clinical efficacy of Gao's nape acupuncture plus swallowing training in treating pharyngeal deglutition disorder after stroke. Methods: One hundred patients with post-stroke pharyngeal deglutition disorder were randomized into a treatment group and a control group, with 50 cases in each group. The two groups both received routine neurological intervention. In addition, the treatment group was given Gao's nape acupuncture plus swallowing training, while the control group was intervened by swallowing training alone. After eight-week treatment, the two groups were observed in terms of the changes in repetitive saliva swallowing test (RSST), modified water swallowing test (MWST), standardized swallowing assessment (SSA) and swallowing-related quality of life (SWAL-QOL). The clinical efficacies of the two groups were also compared. Results: After treatment, the RSST grading, and scores of MWST, SSA and SWAL-QOL changed significantly in both groups (P<0.05 or P<0.01). The RSST grading, and scores of MWSX SSA and SWAL-QOL in the treatment group were significantly different from those in the control group after treatment (P<0.05 or P<0.01). The total effective rate and markedly effective rate were respectively 100.0% and 72.3% in the treatment group, versus 97.9% and 34.0% in the control group. There was a significant differenee in the markedly effective rate between the two groups (P<0.01). The differenee in the clinical efficacy between the two groups was statistically significant (P<0.01). Con elusion: Gao's n ape acupu ncture plus swallowi ng training is an effective approach for post-stroke phary ngeal deglutiti on disorder. Its therapeutic efficacy is more significant than that of swallowing training alone.
