The effects of temperature, initial pH, salinity of culture medium, and carbon and nitrogen sources on growth and doco- sahexaenoic acid (C22 : 6 n-3, DHA) production from Schizochytrium limacinum OUC88 were investiga...The effects of temperature, initial pH, salinity of culture medium, and carbon and nitrogen sources on growth and doco- sahexaenoic acid (C22 : 6 n-3, DHA) production from Schizochytrium limacinum OUC88 were investigated in the present study. The results revealed that the optimal temperature, initial pH and salinity level of the medium for DHA production were 23℃, 7.0 and 18, respectively. Glucose was proved the best carbon source for the growth and DHA production from S. limacinum. Among the nitrogen sources tested, soybean cake hydrolysate, a cheap by-product, was found to be effective for the accumulation of DHA in S. lima- cinum cells. In addition, increasing the concentration of carbon sources in the medium caused a significant increase in cell biomass; however, accumulation of DHA in cells was mainly stimulated by the ratio of C/N in the medium. Under the optimal culture condi- tions, the maximum DHA yield achieved in flasks was 4.08 g L-1 after 5 d of cultivation.展开更多
Volvariella volvacea is a popular variety because of its distinct flavor, pleasant taste, higher protein content and shorter cropping duration compared with other cultivated mushrooms. It is also the most expensive mu...Volvariella volvacea is a popular variety because of its distinct flavor, pleasant taste, higher protein content and shorter cropping duration compared with other cultivated mushrooms. It is also the most expensive mushroom in artificial mushrooms.With the development of economy and the industrialization of edible fungi, more and more straw mushroom has been cultivated in northern China, and southern mushroom is moving to northern areas. In this study, based on many years of cultivation experience, the high yield cultivating techniques of V. volvacea was summarized. V. volvacea cultivation consists of five steps, i.e.,composting, pasteurization, spawning, pining and harvesting.展开更多
Aflatoxins are produced mainly by Aspergillus flavus and Aspergillus parasiticus, and can be found in many grains such as peanuts, soybeans and com. This study aimed to qualitatively and quantitatively evaluate the pr...Aflatoxins are produced mainly by Aspergillus flavus and Aspergillus parasiticus, and can be found in many grains such as peanuts, soybeans and com. This study aimed to qualitatively and quantitatively evaluate the production of aflatoxin in liquid media using strains of Aspergillus flavus obtained from peanuts marketed in the city of Fortaleza, CEo Strains of Aspergillus flavus were inoculated into a liquid medium malt extract and after 2 days inoculated into a second medium containing sucrose 5%, MgSO4·7H20 0.1%, KH2PO4 1%, ZnSO4·7H2O 0.0176 g, and cultured for 3 more days. The media were kept at room temperature ranging from 24°C to 32 °C with agitation of 130 rpm and aeration of 4.17 Llmin. Qualitative analysis was performed by thin layer chromatography and quantitatively by high performance liquid chromatography with fluorescence detection, demonstrating the production of aflatoxin B I （588 mg/L） and B2 （929 mg/L）.展开更多
Nowadays many colleges have opened the animation specialty.However, setting up colleges increasing Numbers represent only the surface of the stack, and the nature of the talent cultivation system structure.Lack of opt...Nowadays many colleges have opened the animation specialty.However, setting up colleges increasing Numbers represent only the surface of the stack, and the nature of the talent cultivation system structure.Lack of optimization training mode, curriculum system hasn＇t been established, and the required talent market imbalances, as non-existent, without water fish, animation talent will to die, animation industry will slump and slump, animation market will also be barren and desolate.From in-depth exploration on the teaching system and teaching methods, research, and discuss the best solution, it is necessary to explore the training mode for animation professional development. With animation professional rapid development, the number of students has ballooned, animation education in colleges and universities has become the main base of animation talent cultivation, will in the next few years as China＇s animation industry to develop a large number of high-quality talent.Many schools use the traditional teaching methods and teaching mode, the lack of systematic and innovation.This high-volume, homogeneity of animation education, bring extensive form -- the result of the students＇ professional skills in the face of employment when the choice is very single, many students just after finish this course will use software or it is an imitation of making a few small animation, creative and independent production does not have the ability of some animation short film, at the same time not good to achieve docking of shortage of the professional and market and can not adapt in the work.This will lead to the animation education market, the rapid expansion of saturation will endanger the whole animation industry market. But, in recent years, there is a seemingly contradictory phenomenon： on the one hand, find a job in a large number of animation in colleges and universities graduates appear difficult situation; The domestic animation companies, on the other hand, it is difficult to hire the best animation talent, animation professional obtain employment situation is very serious.For start animation professional colleges and universities, how to stay awake and calm in the boom, it is necessary for us to explore suitable for animation professional development model of education.展开更多
文摘The effects of temperature, initial pH, salinity of culture medium, and carbon and nitrogen sources on growth and doco- sahexaenoic acid (C22 : 6 n-3, DHA) production from Schizochytrium limacinum OUC88 were investigated in the present study. The results revealed that the optimal temperature, initial pH and salinity level of the medium for DHA production were 23℃, 7.0 and 18, respectively. Glucose was proved the best carbon source for the growth and DHA production from S. limacinum. Among the nitrogen sources tested, soybean cake hydrolysate, a cheap by-product, was found to be effective for the accumulation of DHA in S. lima- cinum cells. In addition, increasing the concentration of carbon sources in the medium caused a significant increase in cell biomass; however, accumulation of DHA in cells was mainly stimulated by the ratio of C/N in the medium. Under the optimal culture condi- tions, the maximum DHA yield achieved in flasks was 4.08 g L-1 after 5 d of cultivation.
基金Supported by the Earmarked Fund for Shanghai Modern Edible Fungi-industry Technology Research System(Grant No.201709)
文摘Volvariella volvacea is a popular variety because of its distinct flavor, pleasant taste, higher protein content and shorter cropping duration compared with other cultivated mushrooms. It is also the most expensive mushroom in artificial mushrooms.With the development of economy and the industrialization of edible fungi, more and more straw mushroom has been cultivated in northern China, and southern mushroom is moving to northern areas. In this study, based on many years of cultivation experience, the high yield cultivating techniques of V. volvacea was summarized. V. volvacea cultivation consists of five steps, i.e.,composting, pasteurization, spawning, pining and harvesting.
文摘Aflatoxins are produced mainly by Aspergillus flavus and Aspergillus parasiticus, and can be found in many grains such as peanuts, soybeans and com. This study aimed to qualitatively and quantitatively evaluate the production of aflatoxin in liquid media using strains of Aspergillus flavus obtained from peanuts marketed in the city of Fortaleza, CEo Strains of Aspergillus flavus were inoculated into a liquid medium malt extract and after 2 days inoculated into a second medium containing sucrose 5%, MgSO4·7H20 0.1%, KH2PO4 1%, ZnSO4·7H2O 0.0176 g, and cultured for 3 more days. The media were kept at room temperature ranging from 24°C to 32 °C with agitation of 130 rpm and aeration of 4.17 Llmin. Qualitative analysis was performed by thin layer chromatography and quantitatively by high performance liquid chromatography with fluorescence detection, demonstrating the production of aflatoxin B I （588 mg/L） and B2 （929 mg/L）.
文摘Nowadays many colleges have opened the animation specialty.However, setting up colleges increasing Numbers represent only the surface of the stack, and the nature of the talent cultivation system structure.Lack of optimization training mode, curriculum system hasn＇t been established, and the required talent market imbalances, as non-existent, without water fish, animation talent will to die, animation industry will slump and slump, animation market will also be barren and desolate.From in-depth exploration on the teaching system and teaching methods, research, and discuss the best solution, it is necessary to explore the training mode for animation professional development. With animation professional rapid development, the number of students has ballooned, animation education in colleges and universities has become the main base of animation talent cultivation, will in the next few years as China＇s animation industry to develop a large number of high-quality talent.Many schools use the traditional teaching methods and teaching mode, the lack of systematic and innovation.This high-volume, homogeneity of animation education, bring extensive form -- the result of the students＇ professional skills in the face of employment when the choice is very single, many students just after finish this course will use software or it is an imitation of making a few small animation, creative and independent production does not have the ability of some animation short film, at the same time not good to achieve docking of shortage of the professional and market and can not adapt in the work.This will lead to the animation education market, the rapid expansion of saturation will endanger the whole animation industry market. But, in recent years, there is a seemingly contradictory phenomenon： on the one hand, find a job in a large number of animation in colleges and universities graduates appear difficult situation; The domestic animation companies, on the other hand, it is difficult to hire the best animation talent, animation professional obtain employment situation is very serious.For start animation professional colleges and universities, how to stay awake and calm in the boom, it is necessary for us to explore suitable for animation professional development model of education.
