Phycocyanin, a functional protein found in blue-green algae, with characteristic absorption peak around 620 nm, can be used to detect the quantity of blue-green algae in waters. Spectral characteristics of phycocyanin...Phycocyanin, a functional protein found in blue-green algae, with characteristic absorption peak around 620 nm, can be used to detect the quantity of blue-green algae in waters. Spectral characteristics of phycocyanin were studied by measuring hyperspectral water leaving radiance and absorption curve, and the results showed that the absorption peak of phycocyanin around 620 nm was evident in the curve of water leaving radiance. Bio-optical model of phycocyanin was set up with analytical methods and calibrated with error analysis. Linear relationship between phycocyanin concentration measured and that retrieved with calibrated bio-optical model (R = 0.755) was better than that retrieved with the band ratio of 709 nm/620 nm (R = 0.729), which proved that the calibration was neces-sary for improving the accuracy of phycocyanin concentration.展开更多
基金Acknowledgements The authors would like to thank Dr. Zhang Ytmlin for measuring absorption of blue-green algae, and Zhang Xiaoyu, Zhang Xiaofeng, Liu Tao for field measurements. This work was supported by the National Natural Science Foundation of China （Grant No. 40576078）, and Natural Science Foundation of Guangdong Province （Grant No. 5003685）.
文摘Phycocyanin, a functional protein found in blue-green algae, with characteristic absorption peak around 620 nm, can be used to detect the quantity of blue-green algae in waters. Spectral characteristics of phycocyanin were studied by measuring hyperspectral water leaving radiance and absorption curve, and the results showed that the absorption peak of phycocyanin around 620 nm was evident in the curve of water leaving radiance. Bio-optical model of phycocyanin was set up with analytical methods and calibrated with error analysis. Linear relationship between phycocyanin concentration measured and that retrieved with calibrated bio-optical model (R = 0.755) was better than that retrieved with the band ratio of 709 nm/620 nm (R = 0.729), which proved that the calibration was neces-sary for improving the accuracy of phycocyanin concentration.
