研究高分子量聚乙烯(H igh m o lecu lar w e igh t po lyethy lene,HMW P)材料对成骨细胞活性及其在骨折愈合过程中对新骨形成的影响。(1)体外实验:在HMW P浸提液和标准培养基中培养成人骨髓源成骨细胞,采用M TT法检测成骨细胞的活性...研究高分子量聚乙烯(H igh m o lecu lar w e igh t po lyethy lene,HMW P)材料对成骨细胞活性及其在骨折愈合过程中对新骨形成的影响。(1)体外实验:在HMW P浸提液和标准培养基中培养成人骨髓源成骨细胞,采用M TT法检测成骨细胞的活性、碱性磷酸酶试剂盒检测成骨细胞的生理功能。(2)体内实验:制作犬股骨远端粉碎性骨折模型,用HMW P板与钢板互锁组合固定,术后3、6、9、12周取材,分别进行HE染色组织学观察及扫描电镜观察HMW P板下骨折端的新骨生成情况。结果发现:(1)采用HMW P浸提液和标准培养基培养的2组成骨细胞,在第2、4、8、14 d的增殖能力和碱性磷酸酶活性无统计学差异,P>0.05;(2)术后不同时间点进行肉眼观察、HE染色组织学观察及扫描电镜观察均可见HMW P板与骨组织之间界面无吸收,HMW P下骨折端的新生骨组织随着时间延长不断成熟。提示:HMW P对成骨细胞的活性及新骨形成均无不良影响,可作为内固定材料应用于骨折的治疗。展开更多
Polymer matrix types of fiber hybrid composites are key factors to improve ballistic impact damage tolerances.Here we report ballistic penetration damages of Kevlar/ultra-high molecular weight polyethylene(UHMWPE)hybr...Polymer matrix types of fiber hybrid composites are key factors to improve ballistic impact damage tolerances.Here we report ballistic penetration damages of Kevlar/ultra-high molecular weight polyethylene(UHMWPE)hybrid composites with thermoplastic polyurethane(PU)matrix.The hybrid composites were penetrated by fragment-simulating projectiles(FSPs)using an air gun impact system.The effects of stacking sequences on the ballistic performance of hybrid composites were analyzed.Two types of specific energy absorption(the energy absorption per unit area density and the energy absorption per unit thickness)were investigated.It was found that the main damage modes of PU hybrid composites were fiber breakage,matrix damage,fiber pullout and interlayer delamination.The instantaneous deformation could not be used as a reference index for evaluating the ballistic performance of the target plate.The energy absorption process of the PU hybrid composites showed a nonlinear pattern.The hybrid structure affected the specific energy absorption of the materials.展开更多
文摘研究高分子量聚乙烯(H igh m o lecu lar w e igh t po lyethy lene,HMW P)材料对成骨细胞活性及其在骨折愈合过程中对新骨形成的影响。(1)体外实验:在HMW P浸提液和标准培养基中培养成人骨髓源成骨细胞,采用M TT法检测成骨细胞的活性、碱性磷酸酶试剂盒检测成骨细胞的生理功能。(2)体内实验:制作犬股骨远端粉碎性骨折模型,用HMW P板与钢板互锁组合固定,术后3、6、9、12周取材,分别进行HE染色组织学观察及扫描电镜观察HMW P板下骨折端的新骨生成情况。结果发现:(1)采用HMW P浸提液和标准培养基培养的2组成骨细胞,在第2、4、8、14 d的增殖能力和碱性磷酸酶活性无统计学差异,P>0.05;(2)术后不同时间点进行肉眼观察、HE染色组织学观察及扫描电镜观察均可见HMW P板与骨组织之间界面无吸收,HMW P下骨折端的新生骨组织随着时间延长不断成熟。提示:HMW P对成骨细胞的活性及新骨形成均无不良影响,可作为内固定材料应用于骨折的治疗。
基金National Natural Science Foundation of China(No.51875099)。
文摘Polymer matrix types of fiber hybrid composites are key factors to improve ballistic impact damage tolerances.Here we report ballistic penetration damages of Kevlar/ultra-high molecular weight polyethylene(UHMWPE)hybrid composites with thermoplastic polyurethane(PU)matrix.The hybrid composites were penetrated by fragment-simulating projectiles(FSPs)using an air gun impact system.The effects of stacking sequences on the ballistic performance of hybrid composites were analyzed.Two types of specific energy absorption(the energy absorption per unit area density and the energy absorption per unit thickness)were investigated.It was found that the main damage modes of PU hybrid composites were fiber breakage,matrix damage,fiber pullout and interlayer delamination.The instantaneous deformation could not be used as a reference index for evaluating the ballistic performance of the target plate.The energy absorption process of the PU hybrid composites showed a nonlinear pattern.The hybrid structure affected the specific energy absorption of the materials.
