Objective: To explore water soluble metabolite features of brain tumor specimens with HRMAS-1HMRS and its potential clinical value. Methods: There were thirty cases of pathologically proven brain tumor, including 6 I-...Objective: To explore water soluble metabolite features of brain tumor specimens with HRMAS-1HMRS and its potential clinical value. Methods: There were thirty cases of pathologically proven brain tumor, including 6 I-II grade astrocy- tomas, 7 III grade anaplastic astrocytomas, 10 IV grade glioblastomas and 7 meningiomas. Used Varian Company 600 MHz spectrometer with the Nano-probe for acquisition HRMAS-1HMRS, which was postprocessed with jMRUI 3.2 version software. These metabolic probability and their ratios to Cr were summed. Results: (1) HRMAS-1HMRS could resolve NAA, PCr/Cr, GPC + PCho + Cho, Glu/Gln, Gly, Tau, Ala, Lac, mI and so on. All samples showed Lac, 6 samples showed unknown single peak at 3.72 ppm or 3.90 ppm. (2) The mean Cho/Cr of 6 I-II grade astrocytomas was 2.42 ± 1.01 (P = 0.003, compared with glioblastoma). The mean Cho/Cr of 7 anaplastic astrocytomas was 3.48 ± 0.59 (P = 0.01, compared with glioblastoma). The Cho/Cr of 10 glioblastomas broadly ranged from 0.9 to 11.3 (mean 5.40 ± 1.23). From I-II grade astrocytoma to glioblastoma, Ala/Cr, Tau/Cr and Gly/Cr trends were increased; the mean Ala/Cr of glioma was 0.31 ± 0.13. (3) Meningiomas showed higher Ala and Cho. Their Cr was lower than that of gliomas. 4/7 cases had no NAA, 3/7 patients had lower NAA. Mean Cho/Cr was 3.56 ± 1.01, Ala/Cr was 0.53 ± 0.28 (P = 0.006, compared with glioma). Conclusion: HRMAS-1HMRS can show further details in vivo MRS, resolve in vivo spectroscopic metabolite of Cho compound and differentiate the extent of benign and ma- lignant glioma. With the increase in the malignant degree of gliomas, Cho, mI, Ala, Tau and Gly will increase. HRMAS-1HMRS is the only method of isotropic spectroscopy for pathological specimens.展开更多
生物组织是基于NMR的代谢组学研究的主要对象之一,广泛应用于分子病理学、毒理学、生物医学等众多领域.但是,为了保证测定的准确,组织的NMR实验往往需要在较低的温度下和较短时间内完成,以防止由于组织内酶的降解和扩散而导致的某些代...生物组织是基于NMR的代谢组学研究的主要对象之一,广泛应用于分子病理学、毒理学、生物医学等众多领域.但是,为了保证测定的准确,组织的NMR实验往往需要在较低的温度下和较短时间内完成,以防止由于组织内酶的降解和扩散而导致的某些代谢物质的分析信息被破坏.统计全相关谱(Statistical Total Correlation Spectroscopy,STOCSY)是依靠一维谱来实现二维谱的一些功能的方法,不需要额外的实验时间,已经被广泛应用于代谢组学研究中.本文采用STOCSY方法,通过对一系列1H高分辨魔角旋转谱的统计分析和计算,得到了肾脏组织的准二维相关谱,其中共振峰之间的相关较为准确的反应了物质之间的耦合信息,为物质的归属提供了帮助.展开更多
基金grants from The National Natural ScienceFoundation of China (10234070, 20573132 and 20575074)The Chinese Academy of Sciences (100T programme: T12508-06S138)~~
文摘Objective: To explore water soluble metabolite features of brain tumor specimens with HRMAS-1HMRS and its potential clinical value. Methods: There were thirty cases of pathologically proven brain tumor, including 6 I-II grade astrocy- tomas, 7 III grade anaplastic astrocytomas, 10 IV grade glioblastomas and 7 meningiomas. Used Varian Company 600 MHz spectrometer with the Nano-probe for acquisition HRMAS-1HMRS, which was postprocessed with jMRUI 3.2 version software. These metabolic probability and their ratios to Cr were summed. Results: (1) HRMAS-1HMRS could resolve NAA, PCr/Cr, GPC + PCho + Cho, Glu/Gln, Gly, Tau, Ala, Lac, mI and so on. All samples showed Lac, 6 samples showed unknown single peak at 3.72 ppm or 3.90 ppm. (2) The mean Cho/Cr of 6 I-II grade astrocytomas was 2.42 ± 1.01 (P = 0.003, compared with glioblastoma). The mean Cho/Cr of 7 anaplastic astrocytomas was 3.48 ± 0.59 (P = 0.01, compared with glioblastoma). The Cho/Cr of 10 glioblastomas broadly ranged from 0.9 to 11.3 (mean 5.40 ± 1.23). From I-II grade astrocytoma to glioblastoma, Ala/Cr, Tau/Cr and Gly/Cr trends were increased; the mean Ala/Cr of glioma was 0.31 ± 0.13. (3) Meningiomas showed higher Ala and Cho. Their Cr was lower than that of gliomas. 4/7 cases had no NAA, 3/7 patients had lower NAA. Mean Cho/Cr was 3.56 ± 1.01, Ala/Cr was 0.53 ± 0.28 (P = 0.006, compared with glioma). Conclusion: HRMAS-1HMRS can show further details in vivo MRS, resolve in vivo spectroscopic metabolite of Cho compound and differentiate the extent of benign and ma- lignant glioma. With the increase in the malignant degree of gliomas, Cho, mI, Ala, Tau and Gly will increase. HRMAS-1HMRS is the only method of isotropic spectroscopy for pathological specimens.
文摘生物组织是基于NMR的代谢组学研究的主要对象之一,广泛应用于分子病理学、毒理学、生物医学等众多领域.但是,为了保证测定的准确,组织的NMR实验往往需要在较低的温度下和较短时间内完成,以防止由于组织内酶的降解和扩散而导致的某些代谢物质的分析信息被破坏.统计全相关谱(Statistical Total Correlation Spectroscopy,STOCSY)是依靠一维谱来实现二维谱的一些功能的方法,不需要额外的实验时间,已经被广泛应用于代谢组学研究中.本文采用STOCSY方法,通过对一系列1H高分辨魔角旋转谱的统计分析和计算,得到了肾脏组织的准二维相关谱,其中共振峰之间的相关较为准确的反应了物质之间的耦合信息,为物质的归属提供了帮助.
