[ Objective] The aim of this study was to discuss the effect of antioxidants and lipid peroxidation from pea crops of plateau. [ Method] SOD enzyme liquid from pea crops of plateau was extracted by means of protein co...[ Objective] The aim of this study was to discuss the effect of antioxidants and lipid peroxidation from pea crops of plateau. [ Method] SOD enzyme liquid from pea crops of plateau was extracted by means of protein concentration assay, enzyme activity assay and antioxidant activity determination by DPPH method, peroxide activity inhibition of in vitro tissues from mice by homogenate MDA colorimetry method and lipid peroxidation assay of in vitro tissues. [ Result ] IC50 of the crude enzyme liquid extracted from pea on DPPH was 55.16 mg/L, while the scavenging rate of the crude enzyme liquid was lower than that of ascorbic acid, tea polyphenol and citric acid with the same concentration. The synergistic effect was found in ascorbic acid and crude enzyme liquid, but the synergism of ascorbic acid was better than that of citric acid. IC50 of SOD enzyme liquid extracted from pea on DPPH was 11.1 mg/L, which was better than that of tea polyphenol and closely similar to that of ascorbic acid. SOD enzyme liquid extracted from pea had an inhibitory effect on MDA production from in vitro tissues such as liver, kidney and heart, especially for a significantly inhibitory effect on MDA from liver in vitro. When the concentration was 0.25 mg/ml, the inhibition rate reached 78.3%, and then the inhibition rate increased little with the concentration incresas, while its effect on heart and kidney were inferior. [ Conclusion] SOD crude enzyme liquid and SOD enzyme liquid extracted from pea all have certain DPPH scavenging capacity, while SOD enzyme liquid extracted from pea has an inhibitory effect on lipid peroxidation.展开更多
Batch experiments tively evaluate the inhibition were conducted to compara- effects and mechanisms of a low-concentration （1%） proline solution cover on the release of pollutants from high-sulfur coal gangue. High- ...Batch experiments tively evaluate the inhibition were conducted to compara- effects and mechanisms of a low-concentration （1%） proline solution cover on the release of pollutants from high-sulfur coal gangue. High- sulfur coal gangue was continuously immersed in a proline solution and in deionized water （as a control treatment） for 540 days. The results showed that the coal gangue in the control treatment was oxidized and generated leachate with poor water qualities, i.e., the leachate exhibited lower pH values, higher redox potential values, higher pollutant concentrations （804^2-, Fe, Mn, Cu, and Zn）, and high levels of acidophilic sulfur-oxidizing bacteria. However, compared to the control treatment, the addition of the proline solution （1%） significantly improved the water quality of the leachate by significantly reducing the Eh values, the pollutant concentrations （804^2-, Fe^2＋, Fe, Mn, Cu, and Zn）, and the activity of acidophilic sulfur-oxidiz- ing bacteria and by significantly increasing the pH value to neutral. The proline treatment significantly inhibited the oxidation of coal gangue and the release of pollutants, mainly by inhibiting the activity of acidophilic sulfur-ox- idizing bacteria and by altering the heavy metal fractions and the mineralogical characteristics. Therefore, in engi- neering practice, workers should consider using an envi- ronmental friendly aqueous proline solution cover to achieve the in-situ control of pollutant releases from coal gangue dumps.展开更多
There has been carried out a comparative research, which allow us to determine the quantities and the central points of accumulation ofPb, Cu, Zn and Cd in the vegetative and reproductive organs of the four sorghum va...There has been carried out a comparative research, which allow us to determine the quantities and the central points of accumulation ofPb, Cu, Zn and Cd in the vegetative and reproductive organs of the four sorghum varieties （technical sorghtan, sugar sorghum, sudan grass and grain sorghnm）, as well as to ascertain the possibilities for their growth in soils contaminated with heavy metals and their application for phytoremediation purposes. The experimental plots were situated at different distances （0.1 and 15.0 kin） from the source of pollution-the Non-Ferrous Metal Works near Plovdiv, Bulgaria. On reaching commercial ripeness, the crops were gathered and the concentrations of Pb, Cu, Zn and Cd in their different parts-roots, stems, leaves and gains-were determined through dry mineralization. To determine the heavy metal content in the samples, ICP was used. A clearly distinguished trend exists which describes the accumulation of heavy metals within the vegetative and reproductive organs of the studied crops. Sudan grass and technical sorghum accumulated larger heavy metal quantities compared to sugar sorghum and grain sorghum, as the majority of the heavy metals was retained by the roots and a very small amount was translocated to epigeous parts. The depots for accumulation were in the following order： roots 〉 leaves 〉 stems 〉 grains. The studied crops can be related to metal-tolerant crops and can be cultivated on softs which are of low, medium or high contamination with lead, zinc and cadmium, as they do not exhibit a tendency of accumulating these elements in grains at levels above the maximum permissible concentrations for fodder. The selective accumulation of Pb, Cu, Zn and Cd in the roots and the possibility to remove the root-remains makes technical sorghum, sugar sorghum and Sudan grass extremely suitable for phytoremediation purposes. The possible use of grains for animal food guarantees the economic expedience upon the selection of these crops.展开更多
文摘[ Objective] The aim of this study was to discuss the effect of antioxidants and lipid peroxidation from pea crops of plateau. [ Method] SOD enzyme liquid from pea crops of plateau was extracted by means of protein concentration assay, enzyme activity assay and antioxidant activity determination by DPPH method, peroxide activity inhibition of in vitro tissues from mice by homogenate MDA colorimetry method and lipid peroxidation assay of in vitro tissues. [ Result ] IC50 of the crude enzyme liquid extracted from pea on DPPH was 55.16 mg/L, while the scavenging rate of the crude enzyme liquid was lower than that of ascorbic acid, tea polyphenol and citric acid with the same concentration. The synergistic effect was found in ascorbic acid and crude enzyme liquid, but the synergism of ascorbic acid was better than that of citric acid. IC50 of SOD enzyme liquid extracted from pea on DPPH was 11.1 mg/L, which was better than that of tea polyphenol and closely similar to that of ascorbic acid. SOD enzyme liquid extracted from pea had an inhibitory effect on MDA production from in vitro tissues such as liver, kidney and heart, especially for a significantly inhibitory effect on MDA from liver in vitro. When the concentration was 0.25 mg/ml, the inhibition rate reached 78.3%, and then the inhibition rate increased little with the concentration incresas, while its effect on heart and kidney were inferior. [ Conclusion] SOD crude enzyme liquid and SOD enzyme liquid extracted from pea all have certain DPPH scavenging capacity, while SOD enzyme liquid extracted from pea has an inhibitory effect on lipid peroxidation.
基金funded by a grant from the United Fund of Guizhou Province GovernmentNational Natural Science Foundation of China(No.U1612442-3)the Project of the Education Department of Guizhou Province(Nos.KY 2016011,GZZ201607,and ZDXK201611)
文摘Batch experiments tively evaluate the inhibition were conducted to compara- effects and mechanisms of a low-concentration （1%） proline solution cover on the release of pollutants from high-sulfur coal gangue. High- sulfur coal gangue was continuously immersed in a proline solution and in deionized water （as a control treatment） for 540 days. The results showed that the coal gangue in the control treatment was oxidized and generated leachate with poor water qualities, i.e., the leachate exhibited lower pH values, higher redox potential values, higher pollutant concentrations （804^2-, Fe, Mn, Cu, and Zn）, and high levels of acidophilic sulfur-oxidizing bacteria. However, compared to the control treatment, the addition of the proline solution （1%） significantly improved the water quality of the leachate by significantly reducing the Eh values, the pollutant concentrations （804^2-, Fe^2＋, Fe, Mn, Cu, and Zn）, and the activity of acidophilic sulfur-oxidiz- ing bacteria and by significantly increasing the pH value to neutral. The proline treatment significantly inhibited the oxidation of coal gangue and the release of pollutants, mainly by inhibiting the activity of acidophilic sulfur-ox- idizing bacteria and by altering the heavy metal fractions and the mineralogical characteristics. Therefore, in engi- neering practice, workers should consider using an envi- ronmental friendly aqueous proline solution cover to achieve the in-situ control of pollutant releases from coal gangue dumps.
文摘There has been carried out a comparative research, which allow us to determine the quantities and the central points of accumulation ofPb, Cu, Zn and Cd in the vegetative and reproductive organs of the four sorghum varieties （technical sorghtan, sugar sorghum, sudan grass and grain sorghnm）, as well as to ascertain the possibilities for their growth in soils contaminated with heavy metals and their application for phytoremediation purposes. The experimental plots were situated at different distances （0.1 and 15.0 kin） from the source of pollution-the Non-Ferrous Metal Works near Plovdiv, Bulgaria. On reaching commercial ripeness, the crops were gathered and the concentrations of Pb, Cu, Zn and Cd in their different parts-roots, stems, leaves and gains-were determined through dry mineralization. To determine the heavy metal content in the samples, ICP was used. A clearly distinguished trend exists which describes the accumulation of heavy metals within the vegetative and reproductive organs of the studied crops. Sudan grass and technical sorghum accumulated larger heavy metal quantities compared to sugar sorghum and grain sorghum, as the majority of the heavy metals was retained by the roots and a very small amount was translocated to epigeous parts. The depots for accumulation were in the following order： roots 〉 leaves 〉 stems 〉 grains. The studied crops can be related to metal-tolerant crops and can be cultivated on softs which are of low, medium or high contamination with lead, zinc and cadmium, as they do not exhibit a tendency of accumulating these elements in grains at levels above the maximum permissible concentrations for fodder. The selective accumulation of Pb, Cu, Zn and Cd in the roots and the possibility to remove the root-remains makes technical sorghum, sugar sorghum and Sudan grass extremely suitable for phytoremediation purposes. The possible use of grains for animal food guarantees the economic expedience upon the selection of these crops.
