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恶性横纹肌样瘤(MRT)的起源研究——高变异率HeLa细胞致裸鼠产生MRT的实验研究 被引量:6
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作者 张德礼 黄高升 +5 位作者 李六金 何旭玉 夏耕田 高步先 白晓鸿 刘尚高 《Acta Genetica Sinica》 SCIE CAS CSCD 2000年第12期1057-1071,T002,共16页
:HeLa细胞KB株、X株、NM20/X株、H株的染色体众数依次为60±3(超二倍体)、62±3(超二倍体)、68±3(超二倍体和亚四倍体)和78±2(亚四倍体),所占比率分别为72%~76%,69%,52%和40%。在纯化3代的肿瘤阴性对... :HeLa细胞KB株、X株、NM20/X株、H株的染色体众数依次为60±3(超二倍体)、62±3(超二倍体)、68±3(超二倍体和亚四倍体)和78±2(亚四倍体),所占比率分别为72%~76%,69%,52%和40%。在纯化3代的肿瘤阴性对照二倍体猫肾(染色体众数38所占比率80%)和犬肾原代细胞皮下接种裸鼠的致癌/致瘤率分别为0%(0/22)和0%(0/10),X株HeLa细胞冻融裂解物皮下接种裸鼠产生进行性缩小肿瘤的比率为20%(1/5)的前提下,HeLa细胞KB株、X株、NM20/X株皮下接种裸鼠产生进行性生长恶性肿瘤的比率分别为100%(10/10),100%(25/25)和100%(5/5),H株细胞皮下接种裸鼠产生恶性肿瘤的比率为50%(5/10)。其中,只有HeLa细胞KB株10~11代(染色体结构畸变率高达20%,出现18%双着丝点和2%断片)以超高数量接种的1组4只裸鼠(0.17ml 12.75×107/鼠)才均形成MRT,特别是从染色体众数不同的3株HeLa细胞中筛选出致瘤性强的X株,连传20代后皮下接种裸鼠形成低分化肿瘤,定名为NM20/X株0代,其染色体众数因经裸鼠体内传代选育而明显增大(染色体众数为68±3,所占比率为52%),实体瘤手术切除后体外连传11代,再皮下接种裸鼠均形成MRT(5/5),但要求完形活细胞接种量要大(5~12×107/鼠),肿瘤产生? 展开更多
关键词 恶性横纹肌样瘤 HELA细胞 高变异率
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拉米夫定治疗慢性乙型肝炎后YMDD变异的处理
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作者 陈丽军 王文冰 《中国冶金工业医学杂志》 2006年第1期99-100,共2页
核苷类似物拉米夫定作用于HBV聚合酶逆转录酶活性部位YMDD基序,快速抑制HBVDNA的复制,是目前治疗慢性乙型肝炎比较理想的药物。但因其不能清除cccDNA,在临床上必须长期用药,随着疗程的延长,高变异率和变异后的耐药限制了其在临床... 核苷类似物拉米夫定作用于HBV聚合酶逆转录酶活性部位YMDD基序,快速抑制HBVDNA的复制,是目前治疗慢性乙型肝炎比较理想的药物。但因其不能清除cccDNA,在临床上必须长期用药,随着疗程的延长,高变异率和变异后的耐药限制了其在临床的广泛应用。国外报道治疗52周变异率达32%。并随疗效延长而增高。针对出现拉米夫定治疗后发生YMDD变异患者的处理进行研究。 展开更多
关键词 YMDD变异 拉米夫定治疗 慢性乙型肝炎 肝炎后 HBVDNA CCCDNA 高变异率 核苷类似物 活性部位 逆转录酶
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60GHz单级功率放大器的设计 被引量:1
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作者 王岗 孙玲玲 文进才 《杭州电子科技大学学报(自然科学版)》 2013年第6期1-4,共4页
该文探讨了60 GHz功率放大器的设计方法,设计并测试了基于0.07μm GaAs工艺的60 GHz毫米波单片功率放大器,该放大器采用共源结构,单级放大,工作电压为1.2 V,工作电流为27 mA,在62.2 GHz时有最大小信号增益4.9 dB,60 GHz时仿真的输出1 d... 该文探讨了60 GHz功率放大器的设计方法,设计并测试了基于0.07μm GaAs工艺的60 GHz毫米波单片功率放大器,该放大器采用共源结构,单级放大,工作电压为1.2 V,工作电流为27 mA,在62.2 GHz时有最大小信号增益4.9 dB,60 GHz时仿真的输出1 dB压缩点功率为12 dBm。 展开更多
关键词 毫米波单片集成电路 砷化镓 放大器 变异电子迁移晶体管
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Effect Analysis of High Strain Rate and Anisotropy on Tension⁃Compression Asymmetry of Aluminum Alloy 7050
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作者 FU Xiuli SHI Qihang +1 位作者 WANG Hui PAN Yongzhi 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI CSCD 2020年第3期377-384,共8页
Using the devices of split Hopkinson tension bar(SHTB)and split Hopkinson pressure bar(SHPB),the dynamic tension and compression experiments in three typical forming directions(rolling direction(RD),transverse directi... Using the devices of split Hopkinson tension bar(SHTB)and split Hopkinson pressure bar(SHPB),the dynamic tension and compression experiments in three typical forming directions(rolling direction(RD),transverse direction(TD)and normal direction(ND))were carried out at strain rates of 1000,2000 and 4000 s-1,respectively.From the microscopic point of view,the effect of strain rate and anisotropy on tension compression asymmetry of aviation aluminum alloy 7050 was studied by scanning electron microscope(SEM),metallographic microscope and electron backscatter diffraction(EBSD).The results showed that there was obvious asymmetry between tension and compression,especially that the yield strength of the material in tension was higher than that in compression.The asymmetry in the elastic stage of tension-compression was weaker and the asymmetry in the strengthening stage was stronger with the increase of strain rate.At the same strain rate,the changing trend of the flow stress was distinct under different orientations of tension and compression,which was related to the stress direction of the grains.According to EBSD grain orientation analysis and raw material texture pole figure analysis,it was found that the larger the difference in the degree of grain refinement during tension and compression,the larger the macro-flow stress difference. 展开更多
关键词 aluminum alloy TEXTURE high strain rate ANISOTROPY tension-compression asymmetry
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乙型肝炎病毒前C/C区基因变异的研究 被引量:1
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作者 杨小娟 吴国荣 《中华实验和临床病毒学杂志》 CAS CSCD 北大核心 2005年第4期M0004-M0004,共1页
关键词 前C/C区基因变异 乙型肝炎病毒 复制过程 生物学特性 高变异率 反转录酶 发病机制 HBV 酶活性 错配
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HEART RATE VARIABILITY DURING HIGH-INTENSITY EXERCISE 被引量:3
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作者 SARMIENTO Samuel GARCIA-MANSO +4 位作者 Juan Manuel MARTIN-GONZALEZ Juan Manuel VA-AMONDE Diana CALDERóN Javier DA SILVA-GRIGOLETTO Marzo Edir 《Journal of Systems Science & Complexity》 SCIE EI CSCD 2013年第1期104-116,共13页
The aim of this paper is to describe and analyse the behaviour of heart rate variability(HRV)during constant-load,high-intensity exercise using a time frequency analysis(Wavelet Transform).Eleven elite cyclists took p... The aim of this paper is to describe and analyse the behaviour of heart rate variability(HRV)during constant-load,high-intensity exercise using a time frequency analysis(Wavelet Transform).Eleven elite cyclists took part in the study(age:18.6±3.0 years;VO_(2max):4.88±0.61 litres·min^(-1)).Initially,all subjects performed an incremental cycloergometer test to determine load power in a constant load-test(379.55±36.02 W;89.0%).HRV declined dramatically from the start of testing(p<0.05).The behaviour of power spectral density within the LF band mirrored that of total energy,recording a significant decrease from the outset LF peaks fell rapidly thereafter,remaining stable until the end of the test.HF-VHF fell sharply in the first 20 to 30 seconds.The relative weighting(%) of HF-VHF was inverted with the onset of fatigue,[1.6%at the start,7.1(p<0.05) at the end of the first phase,and 43.1%(p<0.05) at the end of the test].HF-VHF_(peak) displayed three phases:a moderate initial increase,followed by a slight fall,thereafter increasing to the end of the test.The LF/HF-VHF ratio increased at the start,later falling progressively until the end of the first phase and remaining around minimal values until the end of the test. 展开更多
关键词 CYCLING heart rate variability wavelet.
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