Embryogenic calli were induced from the seeds of creeping bentgrass ( Agrostis palustris Huds.) cv. Regent and colonial bentgrass ( Agrostis Tenuis Sibth. Fl. Oxen.) cv. Tiger. The embryogenic calli were precult...Embryogenic calli were induced from the seeds of creeping bentgrass ( Agrostis palustris Huds.) cv. Regent and colonial bentgrass ( Agrostis Tenuis Sibth. Fl. Oxen.) cv. Tiger. The embryogenic calli were precultured on fresh medium for 4-7 days and then co cultivated with Agrobacterium tumefaciens , LBA4404, which contains plasmid vector pSBGM harboring bar coding region, synthetic green fluorescent protein (sGFP) coding region and matrix attachment region (MAR). After 3 days of co cultivation, the calli were washed thoroughly and transferred to MS medium containing 2 mg/L of 2, 4 D, 12-15 mg/L phosphinothricin (PPT) and 250 mg/L of cefotaxime. After 2-3 months of selection, the actively growing calli of 'Regent' and 'Tiger' were transferred to MS medium with 12-15 mg/L PPT and 250 mg/L cefotaxime for regeneration. The putative transformants were maintained on MS medium with 3 mg/L PPT for long period but control died within 1 month. After establishing in greenhouse, the transformants also showed strong resistance to 0.4% of herbicide Basta but control plants died within 2 weeks. Under confocal microscope, both young leaves and roots showed significant GFP expression. PCR analysis revealed the presence of a DNA fragment of GFP gene at the expected size (380 bp) in the transformants and its absence in a randomly selected control plant.展开更多
The carbon （C） sequestration potential of turfgrass systems has been investigated and demonstrated from several studies. The role of these ecosystems in continental and Mediterranean climates though, is not yet clea...The carbon （C） sequestration potential of turfgrass systems has been investigated and demonstrated from several studies. The role of these ecosystems in continental and Mediterranean climates though, is not yet clearly understood because environmental limiting factors and management intensities can strongly influence the overall C budget. The aim of the present study is to improve the understanding of the mechanisms underlying C fluxes in a turfgrass ecosystem and to assess its C sequestration potential by estimating the annual C budget. NEE （Net Ecosystem Exchange） of turfgrass was calculated in its seasonal variation over one year, and compared between areas characterized by different degrees of maintenance. The C sequestration potential of the turfgrass was investigated in a golf course near Verona （Italy）, adopting a small-chamber enclosure approach. The measurements of gas exchanges between biosphere and atmosphere, permitted to estimate the NEE, as a function of different management intensities. The intensity of management seems to have influence on its C balance. This study needs further research to understand which maintenance variables are determinant on turfgrass C sequestration.展开更多
The aim of this study was to analyze the effects of mechanical perforation of a golf course grassy sward, subject to maintenance machinery traffic and golf players trampling on its compaction and density. The evolutio...The aim of this study was to analyze the effects of mechanical perforation of a golf course grassy sward, subject to maintenance machinery traffic and golf players trampling on its compaction and density. The evolution of soil compaction state after aeration was also conducted in four stages of measurement. This operation aims to improve the structure and soil texture, which is also called ＂perforation＂ or ＂coring＂. The taken cores leaving on the soil holes of adjustable depth and density （350 holes/mE） are made with an aerator machine called Vertidrain. Soil resistance to penetration and density were determined at the initial state before aeration as well as 10, 20, and 30 days after aeration. Compared to the initial state, the results show that mechanical aeration greatly affects the grassy sward ground by reducing its resistance to penetration as 35% and 43% decrease in penetration resistance were noticed at 5 cm depth l0 and 20 days after aeration, respectively. Also, resistance to penetration decreased by 41% and 48% at 15 cm depth during the same two periods of time with a relatively constant moisture content. However, soil resistance to penetration at 5 and 15 cm depths only decreased by 21% and 26%, respectively. Regarding the soil density measured after aeration, a significant improvement at the 1% level with the method of variance analysis was observed compared to that at the initial state （e.g. 1.33 g·cm^-3） Indeed, the density was 1.29, 1.26 and 1.30 gcm^-3 10, 20 and 30 days after aeration, respectively.展开更多
文摘Embryogenic calli were induced from the seeds of creeping bentgrass ( Agrostis palustris Huds.) cv. Regent and colonial bentgrass ( Agrostis Tenuis Sibth. Fl. Oxen.) cv. Tiger. The embryogenic calli were precultured on fresh medium for 4-7 days and then co cultivated with Agrobacterium tumefaciens , LBA4404, which contains plasmid vector pSBGM harboring bar coding region, synthetic green fluorescent protein (sGFP) coding region and matrix attachment region (MAR). After 3 days of co cultivation, the calli were washed thoroughly and transferred to MS medium containing 2 mg/L of 2, 4 D, 12-15 mg/L phosphinothricin (PPT) and 250 mg/L of cefotaxime. After 2-3 months of selection, the actively growing calli of 'Regent' and 'Tiger' were transferred to MS medium with 12-15 mg/L PPT and 250 mg/L cefotaxime for regeneration. The putative transformants were maintained on MS medium with 3 mg/L PPT for long period but control died within 1 month. After establishing in greenhouse, the transformants also showed strong resistance to 0.4% of herbicide Basta but control plants died within 2 weeks. Under confocal microscope, both young leaves and roots showed significant GFP expression. PCR analysis revealed the presence of a DNA fragment of GFP gene at the expected size (380 bp) in the transformants and its absence in a randomly selected control plant.
文摘The carbon （C） sequestration potential of turfgrass systems has been investigated and demonstrated from several studies. The role of these ecosystems in continental and Mediterranean climates though, is not yet clearly understood because environmental limiting factors and management intensities can strongly influence the overall C budget. The aim of the present study is to improve the understanding of the mechanisms underlying C fluxes in a turfgrass ecosystem and to assess its C sequestration potential by estimating the annual C budget. NEE （Net Ecosystem Exchange） of turfgrass was calculated in its seasonal variation over one year, and compared between areas characterized by different degrees of maintenance. The C sequestration potential of the turfgrass was investigated in a golf course near Verona （Italy）, adopting a small-chamber enclosure approach. The measurements of gas exchanges between biosphere and atmosphere, permitted to estimate the NEE, as a function of different management intensities. The intensity of management seems to have influence on its C balance. This study needs further research to understand which maintenance variables are determinant on turfgrass C sequestration.
文摘The aim of this study was to analyze the effects of mechanical perforation of a golf course grassy sward, subject to maintenance machinery traffic and golf players trampling on its compaction and density. The evolution of soil compaction state after aeration was also conducted in four stages of measurement. This operation aims to improve the structure and soil texture, which is also called ＂perforation＂ or ＂coring＂. The taken cores leaving on the soil holes of adjustable depth and density （350 holes/mE） are made with an aerator machine called Vertidrain. Soil resistance to penetration and density were determined at the initial state before aeration as well as 10, 20, and 30 days after aeration. Compared to the initial state, the results show that mechanical aeration greatly affects the grassy sward ground by reducing its resistance to penetration as 35% and 43% decrease in penetration resistance were noticed at 5 cm depth l0 and 20 days after aeration, respectively. Also, resistance to penetration decreased by 41% and 48% at 15 cm depth during the same two periods of time with a relatively constant moisture content. However, soil resistance to penetration at 5 and 15 cm depths only decreased by 21% and 26%, respectively. Regarding the soil density measured after aeration, a significant improvement at the 1% level with the method of variance analysis was observed compared to that at the initial state （e.g. 1.33 g·cm^-3） Indeed, the density was 1.29, 1.26 and 1.30 gcm^-3 10, 20 and 30 days after aeration, respectively.
