The concentrations of polychlorinated biphenyls （PCBs） have been determined in soils collected in Beijing of China. According to Principle Component Analysis （PCA） of PCBs, the possible sources were studied. Relat...The concentrations of polychlorinated biphenyls （PCBs） have been determined in soils collected in Beijing of China. According to Principle Component Analysis （PCA） of PCBs, the possible sources were studied. Relationship between PCBs and soil organic materials revealed that higher chlorinated PCBs are more inclined to be adsorbed by particles rich of organic materials. But the low chlorinated homologues are dominant in Beijing soils, which is very different from the foreign studies. It was concluded that the application of relatively low chlorinated commercial PCBs in China was the main reason leading to this homologue composition character. Besides, the relatively short pollution history is also one of the main reasons. PCA of PCBs data in Beijing soil and atmospheric deposition samples revealed that some other emission sources such as atmospheric deposition, automobile exhaust; chemical and petrochemical industry and steel industry may be the important reasons of elevated PCBs concentrations in Beijing soil展开更多
Revolutionary in scope and application, the CRISPR Cas9 endonuclease system can be guided by 20-nt single guide RNA （sgRNA） to any complementary loci on the double- stranded DNA. Once the target site is located, Cas...Revolutionary in scope and application, the CRISPR Cas9 endonuclease system can be guided by 20-nt single guide RNA （sgRNA） to any complementary loci on the double- stranded DNA. Once the target site is located, Cas9 can then cleave the DNA and introduce mutations. Despite the power of this system, sgRNA is highly susceptible to off-target homologous attachment and can consequently cause Cas9 to cleave DNA at off- target sites. In order to better understand this flaw in the system, the human genome and Streptococcus pyogenes Cas9 （SpCas9） were used in a mathematical and computational study to analyze the probabilities of potential sgRNA off-target homologies. It has been concluded that off-target sites are nearly unavoidable for large-size genomes, such as the human genome. Backed by mathematical analysis, a viable solution is the double-nicking method which has the promise for genome editing specificity. Also applied in this study was a computational algorithm for off-target homology search that was implemented in Java to confirm the mathematical analysis.展开更多
基金Acknowledgements： The research was supported by Beijing Natural and Science Foundation.
文摘The concentrations of polychlorinated biphenyls （PCBs） have been determined in soils collected in Beijing of China. According to Principle Component Analysis （PCA） of PCBs, the possible sources were studied. Relationship between PCBs and soil organic materials revealed that higher chlorinated PCBs are more inclined to be adsorbed by particles rich of organic materials. But the low chlorinated homologues are dominant in Beijing soils, which is very different from the foreign studies. It was concluded that the application of relatively low chlorinated commercial PCBs in China was the main reason leading to this homologue composition character. Besides, the relatively short pollution history is also one of the main reasons. PCA of PCBs data in Beijing soil and atmospheric deposition samples revealed that some other emission sources such as atmospheric deposition, automobile exhaust; chemical and petrochemical industry and steel industry may be the important reasons of elevated PCBs concentrations in Beijing soil
文摘Revolutionary in scope and application, the CRISPR Cas9 endonuclease system can be guided by 20-nt single guide RNA （sgRNA） to any complementary loci on the double- stranded DNA. Once the target site is located, Cas9 can then cleave the DNA and introduce mutations. Despite the power of this system, sgRNA is highly susceptible to off-target homologous attachment and can consequently cause Cas9 to cleave DNA at off- target sites. In order to better understand this flaw in the system, the human genome and Streptococcus pyogenes Cas9 （SpCas9） were used in a mathematical and computational study to analyze the probabilities of potential sgRNA off-target homologies. It has been concluded that off-target sites are nearly unavoidable for large-size genomes, such as the human genome. Backed by mathematical analysis, a viable solution is the double-nicking method which has the promise for genome editing specificity. Also applied in this study was a computational algorithm for off-target homology search that was implemented in Java to confirm the mathematical analysis.
