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反相高效液相色谱法同时测定9种食品中乙酰磺胺酸钾、苯甲酸、山梨酸、糖精钠方法的研究 被引量:10
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作者 李惠彬 《中国卫生检验杂志》 CAS 2007年第7期1173-1175,共3页
目的:建立一种准确、适用的反相高效液相色谱法同时分离测定9种不同类型食品中常用甜味剂乙酰磺胺酸钾、糖精钠和防腐剂苯甲酸、山梨酸的分析方法。方法:不同类型样品应用不同的前处理方法,用Inertsil ODS-C18反相柱分离,HPLC-486UV测定... 目的:建立一种准确、适用的反相高效液相色谱法同时分离测定9种不同类型食品中常用甜味剂乙酰磺胺酸钾、糖精钠和防腐剂苯甲酸、山梨酸的分析方法。方法:不同类型样品应用不同的前处理方法,用Inertsil ODS-C18反相柱分离,HPLC-486UV测定,外标法定量。结果:乙酸磺胺酸钾、苯甲酸、山梨酸、糖精钠在各种样品中的回收率平均值分别为89.6%,92.4%,90.2%,88.8%,其精密度分别为4.56%,0.0015%,1.32%,2.86%,最小检出限在取样量为10.0 g时,分别为0.00012,0.0002,0.0001,0.0004 mg/g。结论:本方法能满足9种食品中乙酰磺胺酸钾、苯甲酸、山梨酸、糖精钠的同时分析测定要求。 展开更多
关键词 高效反液相色谱法 乙酰磺胺酸钾 糖精钠 苯甲酸 山梨酸
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反高效液相色谱法测定地骨皮中大黄素、大黄素甲醚实验研究 被引量:2
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作者 何栋 卢大雷 +3 位作者 刘威峰 徐丹 王卫杰 李惠 《河南中医》 2015年第12期3195-3196,共2页
目的:观察反高效液相色谱法测定地骨皮中大黄素、大黄素甲醚的含量情况。方法:采用Agela C18色谱柱,以甲醇-0.1%磷酸水溶液(85∶15)为流动相,流速1.0 m L·min^(-1),检测波长254 nm,柱温为25℃,对地骨皮中大黄素,大黄素甲醚成分含... 目的:观察反高效液相色谱法测定地骨皮中大黄素、大黄素甲醚的含量情况。方法:采用Agela C18色谱柱,以甲醇-0.1%磷酸水溶液(85∶15)为流动相,流速1.0 m L·min^(-1),检测波长254 nm,柱温为25℃,对地骨皮中大黄素,大黄素甲醚成分含量进行测定。结果:该检测方法在(0.1~25.0)mg·L-1浓度范围内线性关系良好,溶液稳定性、重复性及加样回收率良好。结论:反高效液相色谱法操作简便、准确、重复性好,可以用于地骨皮药材中大黄素、大黄素甲醚含量测定,以控制其质量。 展开更多
关键词 高效液相色谱法 地骨皮 大黄素 大黄素甲醚
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血浆游离氨基酸的柱前衍生反相高效液相色谱分析及其在临床检测中的应用 被引量:18
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作者 郑家概 辜英杰 +4 位作者 闫世平 蔡大川 马丽果 牟德海 李光宪 《分析测试学报》 CAS CSCD 北大核心 2005年第5期22-25,29,共5页
用邻苯二甲醛(OPA)-9-芴基甲氧基羰酰氯(FMOC)柱前衍生反相高效液相色谱法测定了婴幼儿血浆中的游离氨基酸.衍生由自动进样器在线自动完成;氨基酸的衍生物用Hypersil ODS反相柱分离,荧光检测器检测;流动相A为10 mmol·L-1pH7.2的磷... 用邻苯二甲醛(OPA)-9-芴基甲氧基羰酰氯(FMOC)柱前衍生反相高效液相色谱法测定了婴幼儿血浆中的游离氨基酸.衍生由自动进样器在线自动完成;氨基酸的衍生物用Hypersil ODS反相柱分离,荧光检测器检测;流动相A为10 mmol·L-1pH7.2的磷酸盐溶液(PB),含0.5%(ψ)四氢呋喃;流动相B为PB-甲醇-乙腈(体积比50:35:15);用二元线性梯度程序洗脱,0 min时A为100%,B为0%;25 min时A为0%,B为100%.本法在25 min内使22种氨基酸全部得到良好分离,分离度大于1.5,最小检出限为1 nmol·mL-1,在20~300nmol·mL-1浓度范围内,22种氨基酸的峰面积和浓度之间的线性相关系数在0.982~0.999之间,保留时间和峰面积的相对标准偏差分别为0.05%~0.50%和0.7%~2.9%,回收率为98%~101%.用本法在医院送检的血浆样品中检出了高甘氨酸血症、苯丙酮尿症、精氨琥珀酸血症、胍氨酸血症、同型半胱氨酸尿症、枫糖尿症和酪氨酸血症等氨基酸遗传代谢缺陷症. 展开更多
关键词 柱前衍生 卡甘高效液相色谱法 血浆 游离氨基酸 氨基酸代谢缺陷症
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反高效液色谱法测定通络逐瘀丸中芍药苷的含量
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作者 刘家海 《中国医药指南》 2014年第34期62-63,共2页
目的建立通络逐瘀丸的质量控制标准。方法采用反高效液相色谱法对通络逐瘀丸中芍药苷的含量进行测定。结果通络逐瘀丸中芍药苷的含量在0.3082-6.164μg的范围内,线性关系良好,r=0.9999,芍药苷的回收率为99.82%,RSD为0.79%。结论该方法... 目的建立通络逐瘀丸的质量控制标准。方法采用反高效液相色谱法对通络逐瘀丸中芍药苷的含量进行测定。结果通络逐瘀丸中芍药苷的含量在0.3082-6.164μg的范围内,线性关系良好,r=0.9999,芍药苷的回收率为99.82%,RSD为0.79%。结论该方法操作易行,稳定可靠,重复性好,可以用于通络逐瘀丸的质量控制。 展开更多
关键词 通络逐瘀丸 芍药苷 高效液相色谱法
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Stability of Baicalin Aqueous Solution by Validated RP-HPLC 被引量:6
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作者 仇峰 唐星 +1 位作者 何仲贵 李好枝 《Journal of Chinese Pharmaceutical Sciences》 CAS 2004年第2期134-137,共4页
Aim In the present study a RP-HPLC method was developed and validated toinvestigate the stability of baicalin aqueous solution. Methods The influences of temperature and pHon the stability of baicalin aqueous solution... Aim In the present study a RP-HPLC method was developed and validated toinvestigate the stability of baicalin aqueous solution. Methods The influences of temperature and pHon the stability of baicalin aqueous solution were investigated by classic homoiothermicacceleration test, and the pH for the most stable solution was determined. Results The time whenbaicalin suffered 10% loss was found to be 18.1 h, and the degradation activation energy of baicalinwas 79.1 kJ·moL^(-1) . The pH at which baicalin is most stable is 4.28. Conclusion The temperatureshould be kept at a lower level and the pH should be adjusted to near that for the most stablesolution in the production of baicalin preparations. 展开更多
关键词 RP-HPLC BAICALIN STABILITY TEMPERATURE PH
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RP-HPLC Determination of Pinoresinol Diglucopyranoside in Qing′e Pill Extract 被引量:4
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作者 熊志立 罗璇 +1 位作者 李小芩 李发美 《Journal of Chinese Pharmaceutical Sciences》 CAS 2004年第2期138-141,共4页
Aim To develop and determine pinoresinol diglucopyranoside in Qing'e Pill, atraditional Chinese compound preparation containing Eucommia ulmoides Oliv. as the principal drug,by a reverse-phase high-performance liq... Aim To develop and determine pinoresinol diglucopyranoside in Qing'e Pill, atraditional Chinese compound preparation containing Eucommia ulmoides Oliv. as the principal drug,by a reverse-phase high-performance liquid chromatographic method (RP-HPLC) . Methods The extract ofQing'e Pill was refluxed with 75% ethanol, purified on an AB-8 macroporous adsorption resin columnand then injected into HPLC system. The HPLC assay was performed on an ODS analytical column with amixture of methanol-acetonitrile-water (24:3:78, V/V/V) as the mobile phase at a flow-rate of 1.0mL·min^(-1), and a UV detector set at 227 nm. Results Good linearity between peak area andconcentration was found in the range of 5.5 - 170 μg·mL^(-1) for pinoresinol diglucopyranoside ( r> 0.9998) . The average recovery was 99.3%. The intra-day assay RSD and the inter-day assay RSDwere 1.3% and 2.8%, respectively (n = 5). The content of pinoresinol diglucopyranoside in Qing'ePill was determined to be 0.446 +- 0.012 mg·g^(-1) (n = 10). Conclusion The RP-HPLC method wasproved to be sensitive, specific, accurate and precise for the determination of pinoresinoldiglucopyranoside in Qing' e Pill. 展开更多
关键词 RP-HPLC pinoresinol diglucopyranoside qing'e pill
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RP-HPLC determination of lycopene in microcapsules 被引量:2
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作者 卢定强 陆晓云 +1 位作者 陆凯丰 胡继军 《Journal of Chinese Pharmaceutical Sciences》 CAS 2007年第1期65-68,共4页
Aim A RP- HPLC method for determination of lycopene in microcapsules was established. Methods The HPLC assay was performed on an Alltima Cls (4.6 mm × 250 mm, 5μm) column with a mixture of methanol-THF-water ... Aim A RP- HPLC method for determination of lycopene in microcapsules was established. Methods The HPLC assay was performed on an Alltima Cls (4.6 mm × 250 mm, 5μm) column with a mixture of methanol-THF-water (66:30:4, V/V/V) as mobile phase at a flow rate of 1.5 mL·min^-1 and the UV detection wavelength was 472 nm. Results The linear range of lycopene was 3.6-18 μg·mL^-1, r = 0.999 8, the average recovery was from 99.81% to 101.06% with RSD less than 1.83%. The RSD of intra-day and interday precision were less than 3.34%. Conclusion The method is simple, accurate and suitable for the determination of lycopene in microcapsules. 展开更多
关键词 RP-HPLC LYCOPENE MICROCAPSULES
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Determination of Sulphonylurea Glimepiride in Dog Serum by RP-HPLC with Pre-column Derivatization 被引量:1
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作者 卢来春 蒋学华 +1 位作者 周静 杨俊毅 《Journal of Chinese Pharmaceutical Sciences》 CAS 2003年第2期101-105,共5页
Aim A simple, sensitive and rapid RP HPLC method with pre column derivatization has been developed for the determination of sulphonylurea glimepiride in dog serum. Methods The sulphonylurea glimepiride was extract... Aim A simple, sensitive and rapid RP HPLC method with pre column derivatization has been developed for the determination of sulphonylurea glimepiride in dog serum. Methods The sulphonylurea glimepiride was extracted from the dog serum using dichlromethane followed by derivatization with DNBF for 20 min at 100℃. The solvent was then evaporated at 60℃ under nitrogen, and the residue was taken up in 100 μL of mobile phase consisting of acetonitrile water (75∶30, v/v). The separation was performed on a Hypersil BDS C18 column with a flow rate of 0 8 mL·min -1 , and the ultraviolet detector wavelength was set at 350 nm. Results Extraction recovery ranged from 75.9% to 83.2%, and methodological recovery was between 96.5% and 109.3%. Within day RSD ranged from 1.5% to 6.3%, and inter day RSD was between 2 9% and 14.8%. The method showed good linearity (R=0.9998). Conclusion The method was simple, convenient and sensitive. The reaction of derivatization was reproducible. 展开更多
关键词 GLIMEPIRIDE pre column derivatization DNBF serum concentration
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Hypertonic stimulation induces synthesis and release of glutamate in cultured rat hypothalamic astrocytes and C6 cells 被引量:1
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作者 曹荣 江山 +3 位作者 段丽 熊鹰飞 高蓓 饶志仁 《Neuroscience Bulletin》 SCIE CAS CSCD 2008年第6期359-366,共8页
Objective To investigate whether hypertonic saline (HS) can induce the synthesis and release of glutamate in cultured hypothalamic astrocytes or C6 cell line. Methods Astrocytes were isolated, cultured, purified and... Objective To investigate whether hypertonic saline (HS) can induce the synthesis and release of glutamate in cultured hypothalamic astrocytes or C6 cell line. Methods Astrocytes were isolated, cultured, purified and identified from the hypothalamus of newborn rat (1 day). The astrocytes were randomly divided into five groups: isotonic (IS) and HS groups, astrocytes were incubated by IS and HS (320 mosM NaCl) medium, respectively, for 1, 3, 5, 10 or 15 rain; carbenoxolone (CBX) +IS and CBX+HS groups, astrocytes were pre-treated with CBX (100 mmol/L) for 1 h at 37℃ in a 5% CO2 / 95% atmosphere, then removed to IS and HS medium, respectively, for 1, 3, 5, 10 or 15 min; Ca2++HS group, astrocytes were pre-incubated with Ca2+ (1 000 μmol/L) for 1 h at 37℃ in a 5% CO2 / 95% atmosphere, followed by a wash with isotonic FBS/DMEM, and then removed to hypertonic saline for 1, 3, 5, 10 or 15 min. The media of five groups were collected to analyze the medium glutamate concentration with high performance liquid chromatography. The astrocytes were fixed and double immunofluorescent stained with anti-glial fibrillary acidic protein (GFAP) and anti-glutamate. The C6 cells were divided into four groups: IS, HS, CBX+IS and CBX+HS groups, and used for quantitative measurement of glutamate in cells by flow cytometry (FCM). Results (1) Anti-GFAP immunofluorescent signal revealed no significant difference among various time points in each group, or among the five groups. (2) The anti-glutamate immunofluorescent signal was increased in HS group and peaked at 5 min, and decreased and returned to the level of IS group at 15 rain (P 〈 0.01 vs the 5 min of HS group). In CBX+HS group, the glutamate intensity was higher than that in CBX+IS and HS groups. (3) The medium glutamate concentration had no change after treatment with HS for 1 and 3 min, while increased markedly after treatment for 5 min to 15 min (P 〈 0.01 vs 1 min and 3 min). On the contrary, the medium glutamate concentrations in the CBX+HS or Ca2++HS group were significant lower than that in the HS group (P 〈 0.01). (4) FCM showed HS and CBX+HS induced glutamate increase in C6 cells. Conclusion HS induced cultured rat hypothalamic astrocytes or C6 cells to synthesize and release glutamate; CBX could block glutamate release, but could not disrupt glutamate synthesis. 展开更多
关键词 ASTROCYTES hypertonic stimulation CARBENOXOLONE connexin 43 high performance liquid chromatography immu-nofluorescent stain RAT
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Determination of Candicidin/FR-008 and Related Components in Fermentation Broth by RP-HPLC
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作者 毛相朝 沈亚领 +2 位作者 魏东芝 陈实 邓子新 《Journal of Chinese Pharmaceutical Sciences》 CAS 2005年第2期115-118,共4页
Aim A liquid chromatographic method for the determination ofcandicidin/FR-008 and related components in fermentation broth has been developed. Methods Therewere four major components in the candicidin/FR-008 complex, ... Aim A liquid chromatographic method for the determination ofcandicidin/FR-008 and related components in fermentation broth has been developed. Methods Therewere four major components in the candicidin/FR-008 complex, which were separated by HPLC under thefollowing conditions: SB-C8 column (4.6 mm x 250 mm, 5 μm) was used, the mobile phase consisted ofacetonitrileam-monium acteate (20 mmol·L^(-1) , pH 4.0) (40:60, V/V) , with a flow rate of 1 .0mL·min^(-1) , the UV detection wavelength was 380 nm, and the whole process was performed at 25℃ .Results The linearity was obtained in the range of 6.25 - 500 μg· mL^(-1) candicidin/FR-008 withthe regression equation of Y = 20 461 x + 30 748 and the correlation coefficient of 0.999 1. Theinstrument precision was 1.84% and the method precision was 3.8%. Conclusion This method isaccurate, rapid and simple; it can be used for determination of candicidin/FR-008 and relatedcomponents in fermentation broth. 展开更多
关键词 candicidin/FR-008 HPLC fermentation broth METABOLITES
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A Pre-column Derivatization HPLC Method for the De-termination of Peimine and Peiminine in Bulbus Fritillar-iae
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作者 李文彦 毕开顺 +2 位作者 乔延江 宁黎丽 罗旭 《Journal of Chinese Pharmaceutical Sciences》 CAS 1996年第4期213-218,共6页
A pre-column derivatization HPLC method for the determination of peimine(P)and peiminine(PE)in Bulbus Fritillariae has been developed. Under the derivatization conditions optimized,the calibration curve is Y1=-3.83... A pre-column derivatization HPLC method for the determination of peimine(P)and peiminine(PE)in Bulbus Fritillariae has been developed. Under the derivatization conditions optimized,the calibration curve is Y1=-3.83×103+1.33 ×105X1,r=0.998 for P and Y2=-7.86 × 102+6.33 × 104X2,r=0995 for PE,where Y is the peak area and X is the weight of the alkaloid; the average recovery is 98.0%(n=5, RSD=2.1%) for P and 101.0%(n=5,RSD=4.1%)for PE,the linear range is from 0.504 μg to 3.126μg for P and from 0.520μgto 3.328μgfor PE,respectively. Results of the determination of the two alkaloids in several samples of different Fritillaria species from various parts ofthe country are presented.The results suggest that P and PE are two major chemical constituents in bulbs of different Fritillaria species,and that the method developed is generally appli-cable to the determination of the hydroxy group on aliphatic fused ring systems without steric hin-drance. 展开更多
关键词 Peimine(P) Peiminine(PE) DERIVATIZATION HPLC
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Determination of Lycopene Concentration in Dog Plasma by RP-HPLC
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作者 王会娟 李馨儒 +1 位作者 黄燕清 刘艳 《Journal of Chinese Pharmaceutical Sciences》 CAS 2004年第4期259-261,共3页
A method for determination of lycopene concentration in dog plasma wasestablished. Methods RP-HPLC was used; the mobile phase consisted of methanol-acetonitrile-methylenechloride (40:30:30, V/V) , the wavelength of de... A method for determination of lycopene concentration in dog plasma wasestablished. Methods RP-HPLC was used; the mobile phase consisted of methanol-acetonitrile-methylenechloride (40:30:30, V/V) , the wavelength of detection was 472 nm, the column temperature wasambient temperature, and the flow rate was 1.0 mL·min^(-1). Results The standard curve was linearin the range from 0.012 4 to 0.496 μg·mL^(-1) with r=0.9992. The average extraction recovery was97.6% +-4.2%. The intra-day and inter-day RSD were 1.52% -4.95% and 2.31% -7.38%, respectively.Conclusion This method is sensitive, rapid, reproducible, and of good selectivity for the analysisof lycopene in dog plasma. 展开更多
关键词 LYCOPENE plasma concentration RP-HPLC
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清热汤剂中黄芩苷的含量测定 被引量:4
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作者 谢萍 王艳华 毕开顺 《中国中药杂志》 CAS CSCD 北大核心 2002年第1期67-68,共2页
关键词 清热汤剂 黄芩苷 含量测定 高效液相色谱法
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Simultaneous Determination of Ceftazidime and Tazobactam in Injectable Powder by Reversed-Phase High Performance Liquid Chromatography 被引量:2
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作者 孟湘明 孟志云 +1 位作者 张亮 窦桂芳 《Journal of Chinese Pharmaceutical Sciences》 CAS 2004年第4期267-270,共4页
A reversed-phase high performance liquid chromatographic (RP-HPLC) method wasdeveloped and validated for the simultaneous deteimination of ceftazidime and tazobactam ininject-able powder. Methods Chromatography was ca... A reversed-phase high performance liquid chromatographic (RP-HPLC) method wasdeveloped and validated for the simultaneous deteimination of ceftazidime and tazobactam ininject-able powder. Methods Chromatography was carried out on Zorbax 300SB-C_(18) column using amixture of methanol and aqueous solution of phosphate buffer (pH = 5.6) as mobile phase. The UVdetection wavelength was 220 run. Results The linear ranges of ceftazidime and tazobactam were 0.62- 631.8 μg·mL^(-1) and 0.66 - 677.50 μg·mL^(-1), respectively. The average recoveries were 98.8%- 101.4% for ceftazidime, and 99,1% - 100.2% for tazobactam. The RSD values of inter-day andintra-day assays were lower than 1.5% for ceftazidime and 2.6% for tazobactam. Conclusion Thismethod is reproducible, simple, precise, and rapid for the quality control of ceftazidime andtazobactam in injectable powder. 展开更多
关键词 CEFTAZIDIME TAZOBACTAM RP-HPLC
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RP-HPLC法测定生地黄和酒地黄不同提取方法中梓醇的含量 被引量:5
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作者 杨昌庆 殷秀丽 +1 位作者 宋海荣 孙艳涛 《广东化工》 CAS 2016年第20期162-164,共3页
目的:比较生地黄和熟地黄中梓醇的含量;探究炮制对地黄中梓醇含量的影响。方法:回流提取法;反高效液相色谱法,采用C18柱,流动相为水-乙腈(1∶99),流速为1.0 m L·min-1,检测波长为210 nm。结果:70%乙醇30倍量提取时,测得生地黄中梓... 目的:比较生地黄和熟地黄中梓醇的含量;探究炮制对地黄中梓醇含量的影响。方法:回流提取法;反高效液相色谱法,采用C18柱,流动相为水-乙腈(1∶99),流速为1.0 m L·min-1,检测波长为210 nm。结果:70%乙醇30倍量提取时,测得生地黄中梓醇的含量最高;70%乙醇10倍量提取时,测得酒地黄中的梓醇含量最高。结论:生地黄与酒地黄中梓醇的含量不同,不同浓度和倍量的乙醇影响地黄中梓醇的提取。 展开更多
关键词 酒地黄 梓醇 回流提取法 高效液相色谱法 中药炮制
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A RP-HPLC method for determination of paclitaxel in its solid dispersion
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作者 刘祥瑞 吴科春 +2 位作者 张春晖 张烜 张强 《Journal of Chinese Pharmaceutical Sciences》 CAS 2007年第2期101-104,共4页
Aim To develop and validate a RP-HPLC method for the analysis of paclitaxel in a solid dispersion. Methods Paclitaxel and the internal standard norethisterone were separated using a Phenomenex ODS 3 column and monitor... Aim To develop and validate a RP-HPLC method for the analysis of paclitaxel in a solid dispersion. Methods Paclitaxel and the internal standard norethisterone were separated using a Phenomenex ODS 3 column and monitored at a wavelength at 227 nm. The isocratic mobile phase consisting of methanol-acetonitrile-water (40:30:30, V/V) was pumped at a flow-rate of 1.0 mL·min^-1. The dissolution studies were performed according to published studies. Results Under these chromatographic conditions, the calibration curve was linear in the range of 4-40 μg·mL ^-1 with the correlation coefficient of 0.9999. The mean recovery was 98.42 % (RSD = 1.19 %). At the 60 min time point, the dissolution of paclitaxel from the solid dispersion was nearly 100 %, however, the original form of paclitaxel was about 30 %. Conclusion The method was proven to be specific, accurate and precise for determining the dissolution of paclitaxel from solid dispersion. 展开更多
关键词 PACLITAXEL Solid dispersion RP-HPLC
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Development and Validation of a Novel Reverse Phase HPLC for Determination of Aloperine in Aloperine Tablets 被引量:4
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作者 WANGHua ZHENGLi WANGZhan ZHANGYong-qiang] WUFeng-lan 《Journal of Chinese Pharmaceutical Sciences》 CAS 2003年第4期211-214,共4页
Aim To develop a reverse phase HPLC method for the determination of aloperine, an alkaloid that is newly extracted from Sophora alopecuraides and has shown wide pharmacological effects including antibacterial and... Aim To develop a reverse phase HPLC method for the determination of aloperine, an alkaloid that is newly extracted from Sophora alopecuraides and has shown wide pharmacological effects including antibacterial and antiinflammatory actions. Methods The samples were analyzed on a ODS column with methanol water triethylamine (3∶97∶0 1 V/V) as a mobile phase. The flow rate was 1 0 mL·min -1 , and UV detection wavelength 205 nm. Results Linear regression equation was A=1 6920C+1 7455 (r 2=0 9999, n =5) in concentratins ranging from 20 to 120 μg·mL -1 . The recoveries were 101 2±1 46 % at 80 μg·mL -1 , 100 5±0 75% at 100 μg·mL -1 , and 100 7±1 10% at 120 μg·mL -1 , respectively, and the precisions of aloperine within or between run were from 0 80% to 1 98% ( n =5). The relative contents of aloperine in three lots of tablets were 101 59±1 38%, 98 46±0 23%, and 99 41±1 09% ( n =3). Conclusion The newly developed reverse phase HPLC method is simple and useful for daily assay of aloperine tablets and can overcome the interference from excipient and other alkaloids in titration and UV detection. 展开更多
关键词 ALOPERINE aloperine tablets RP HPLC
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Decreased blood riboflavin levels are correlated with defective expression of RFT2 gene in gastric cancer 被引量:6
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作者 Maynur Eli De-Sheng Li +6 位作者 Wei-Wei Zhang Bing Kong Chen-Song Du Maimaitiaili Wumar Batur Mamtimin Ilyar Sheyhidin Ayshamgul Hasim 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第24期3112-3118,共7页
AIM:To investigate the relationship between blood riboflavin levels and riboflavin transporter 2(RFT2) gene expression in gastric carcinoma(GC) development.METHODS:High-performance liquid chromatography was used to de... AIM:To investigate the relationship between blood riboflavin levels and riboflavin transporter 2(RFT2) gene expression in gastric carcinoma(GC) development.METHODS:High-performance liquid chromatography was used to detect blood riboflavin levels in patients with GC.Real-time fluorogenic quantitative polymerase chain reaction and immunohistochemistry were used to analyze the expression of RFT2 mRNA and protein in samples from 60 GC patients consisting of both tumor and normal tissue.RESULTS:A significant decrease in the RFT2 mRNA levels was detected in GC samples compared with those in the normal mucous membrane(0.398 ± 0.149 vs 1.479 ± 0.587;P = 0.040).Tumors exhibited low RFT2 protein expression(75%,16.7%,8.3% and 0% for no RFT2 staining,weak staining,medium staining and strong staining,respectively),which was significantly lower than that in the normal mucous membrane(10%,16.7%,26.7% and 46.7% for no RFT2 staining,weak staining,medium staining and strong staining,respectively;P < 0.05).Tumors with low RFT2 expression were significantly associated with tumor stage and histological grade.Moreover,a significantly decrease in Uyghur patients was observed compared with Han patients.However,other parameters-gender,tumor location and lymph node metastasis-showed no significant relationship with RFT2 expression.Blood riboflavin levels were reverse correlated with development of GC(1.2000 ± 0.97 569 ng/mL in high tumor stage patients vs 2.5980 ± 1.31 129 ng/mL in low tumor stage patients;P < 0.05).A positive correlation of plasma riboflavin levels with defective expression of RFT2 protein was found in GC patients(2 = 2.619;P = 0.019).CONCLUSION:Defective expression of RFT2 is associated with the development of GC and this may represent a mechanism underlying the decreased plasma riboflavin levels in GC. 展开更多
关键词 Gastric carcinoma Riboflavin transporter 2gene RIBOFLAVIN Prognosis High-performance liquid chro-matography
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The Use of HPLC in Determination of Endogenous Hormones in Anthers of Bitter Melon 被引量:4
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作者 Yi Tang Li Wang Chao Ma Ji Liu Bin Liu Huanxiu Li 《Journal of Life Sciences》 2011年第2期139-142,共4页
A simple, rapid and perfect extraction and determination method for the endogenous hormones in anthers of bitter melon with reversed-phase high performance liquid chromatography (HPLC) has been developed. The HPLC s... A simple, rapid and perfect extraction and determination method for the endogenous hormones in anthers of bitter melon with reversed-phase high performance liquid chromatography (HPLC) has been developed. The HPLC system consisted of Hypersil ODS C 18 reverse phase column (150 mm × 4.6 mm, 5 μm) with a methanol gradient in 0.6% acetic acid and UV detector set at 254 nm. The results showed that the method was accurate and efficient. 展开更多
关键词 HPLC endogenous hormones bitter melon (Momordica eharantia L.)
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RP-HPLC法测定小儿清热止咳口服液中黄芩苷的含量 被引量:2
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作者 袁才琼 邱海蕴 +1 位作者 杨国庆 康红英 《中国药师》 CAS 2008年第3期333-334,共2页
目的:建立反相高效液相色谱法测定小儿清热止咳口服液中黄芩苷的含量。方法:采用Diamonsil C_(18)色谱柱(250 mm×4.6 mm,5μm),以甲醇-水-磷酸溶液(50:50:0.3)为流动相,流速1.0 ml·min^(-1),检测波长276 nm。结果:线性范围0.... 目的:建立反相高效液相色谱法测定小儿清热止咳口服液中黄芩苷的含量。方法:采用Diamonsil C_(18)色谱柱(250 mm×4.6 mm,5μm),以甲醇-水-磷酸溶液(50:50:0.3)为流动相,流速1.0 ml·min^(-1),检测波长276 nm。结果:线性范围0.083~0.92μg(r=0.999 9)。平均回收率为95.4%,RSD 0.8%(n=5)。结论:本法简便、准确、重现性好,可用于该制剂质量控制。 展开更多
关键词 高效液相色谱法 黄芩苷 小儿清热止咳口服液
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