Cimicifugae Rhizome （RC） is one of many commonly used traditional Chinese medicines. This study used Box-Behnken Design （BBD）, a widely used form of response surface methodology （RSM）, to optimize the conditions...Cimicifugae Rhizome （RC） is one of many commonly used traditional Chinese medicines. This study used Box-Behnken Design （BBD）, a widely used form of response surface methodology （RSM）, to optimize the conditions of the ultrasound-assisted extraction （UAE） for the extraction of phenolic compounds from CimicifugaeRhizome. The influence of three independent variables, including ethanol concentration （%）, extraction time （min） and solvent-to-material ratio （mL/g）, on the extraction yields of phenolic compounds were examined. The results demonstrated that the optimal ultrasound-assisted extraction （UAE） conditions were as follows： ethanol concentration of 68%, an extraction time of 19 min, and solvent-to-material ratio of 30 mL/g for the extraction of caffeic acid, cimicifugoside, ferulic acid, isoferulicacid, cimifugin, 4′-O-β-glucopyranosyl-5-O-methylvisamminol. These results demonstrated the suitability of UAE for the extraction of phenolic compounds from CimicifugaeRhizome.展开更多
As the main bioactive metabolites of Ganoderma lucidum, triterpenoids have various pharmacological effects. In this paper, the nutritional requirements and culture conditions of a submerged culture of G. lucidum were ...As the main bioactive metabolites of Ganoderma lucidum, triterpenoids have various pharmacological effects. In this paper, the nutritional requirements and culture conditions of a submerged culture of G. lucidum were optimized using the response surface methodology; maximum mycelia biomass and intracellular triterpenoid production reached 1.87 g/100 ml and 93.21 mg/100 ml, respectively, for a culture consisting of wort 4.10% （0.041 g/ml） and yeast extract 1.89% （0.0189 g/ml）, pH 5.40. For the first time, we established that wort, which is cheap and abundant, can replace the more commonly used glucose as the sole source of carbohydrate. Using high-performance liquid chromatography-electrospray ionization-mass spectrometry （HPLC-ESI-MS）, 10 major ganoderic acids were tentatively identified based on the predominant fragmentation pathways with the elimination of H20 and CO2, as well as cleavage of the D-ring.展开更多
＂战略伙伴关系＂和＂战略联盟＂已成为互换使用的词。但伙伴关系针对的是在不同阶段供应链中企业的纵向关系商业的成功来自于公司管理和供应链整体性能的提高,也取决于为顾客提供的改进了的服务。随着各部门的竞争转移到供应链间,每个...＂战略伙伴关系＂和＂战略联盟＂已成为互换使用的词。但伙伴关系针对的是在不同阶段供应链中企业的纵向关系商业的成功来自于公司管理和供应链整体性能的提高,也取决于为顾客提供的改进了的服务。随着各部门的竞争转移到供应链间,每个公司正试图构建更加有效和敏捷的供应链。高效消费者响应（E C R）倡议最初是在美国零售业掀起的,随后遍及到整个欧洲。这个范板的转移为食品供应链管理提供了推动力。展开更多
基金Suzhou Youth science and technology plan(Grant No.KJXW2015028)
文摘Cimicifugae Rhizome （RC） is one of many commonly used traditional Chinese medicines. This study used Box-Behnken Design （BBD）, a widely used form of response surface methodology （RSM）, to optimize the conditions of the ultrasound-assisted extraction （UAE） for the extraction of phenolic compounds from CimicifugaeRhizome. The influence of three independent variables, including ethanol concentration （%）, extraction time （min） and solvent-to-material ratio （mL/g）, on the extraction yields of phenolic compounds were examined. The results demonstrated that the optimal ultrasound-assisted extraction （UAE） conditions were as follows： ethanol concentration of 68%, an extraction time of 19 min, and solvent-to-material ratio of 30 mL/g for the extraction of caffeic acid, cimicifugoside, ferulic acid, isoferulicacid, cimifugin, 4′-O-β-glucopyranosyl-5-O-methylvisamminol. These results demonstrated the suitability of UAE for the extraction of phenolic compounds from CimicifugaeRhizome.
基金supported by the National Natural Science Foundation of China(No.31130042)
文摘As the main bioactive metabolites of Ganoderma lucidum, triterpenoids have various pharmacological effects. In this paper, the nutritional requirements and culture conditions of a submerged culture of G. lucidum were optimized using the response surface methodology; maximum mycelia biomass and intracellular triterpenoid production reached 1.87 g/100 ml and 93.21 mg/100 ml, respectively, for a culture consisting of wort 4.10% （0.041 g/ml） and yeast extract 1.89% （0.0189 g/ml）, pH 5.40. For the first time, we established that wort, which is cheap and abundant, can replace the more commonly used glucose as the sole source of carbohydrate. Using high-performance liquid chromatography-electrospray ionization-mass spectrometry （HPLC-ESI-MS）, 10 major ganoderic acids were tentatively identified based on the predominant fragmentation pathways with the elimination of H20 and CO2, as well as cleavage of the D-ring.
文摘＂战略伙伴关系＂和＂战略联盟＂已成为互换使用的词。但伙伴关系针对的是在不同阶段供应链中企业的纵向关系商业的成功来自于公司管理和供应链整体性能的提高,也取决于为顾客提供的改进了的服务。随着各部门的竞争转移到供应链间,每个公司正试图构建更加有效和敏捷的供应链。高效消费者响应（E C R）倡议最初是在美国零售业掀起的,随后遍及到整个欧洲。这个范板的转移为食品供应链管理提供了推动力。
