泸州市博物馆藏高树14件作品之管窥

高树是晚清官员、诗人、书法家、戏曲作家,工书擅文,名震京师。泸州市博物馆所藏高树14件作品,题材主要有随感杂谈、思乡怀亲、唱和酬赠等内容,其中蕴含的历史信息对于研究高树生平、家族、家风家教、交游、学术思想等具有重要的参考价值与补充作用。

《清人诗文集总目提要》订补——以高树等十人生卒年为中心

今人柯愈春先生所著《清人诗文集总目提要》,从清代诗歌(包括散文)文献学的角度来说,代表了迄今为止该领域学术研究的最高成就。因此,以该书作为基准,对其中难免存在的若干舛误与疏漏进行订正与补充,从而使之尽可能地更趋完善,这也就成了一项很有意义的工作。同时,这些遗留下来的问题,其难度相对来说也是最大的。现在即根据平日读书所得,对其中高树、易顺豫、王守恂、江朝宗、成多禄、许钟璐、徐兆光、旷世斌、傅岳棻等十位作家生卒年的有关问题予以订补,供作者及其他相关读者参考。

定高树径测量仪的研究

定高树径测量仪是基于单片机、超声波测距、条形码识别等多种技术而开发研制的在树木确定高度情况下进行测量树木直径的一种新型测量仪器。本课题的主要目的就是研制出一种脱离传统手工记录测量数据的高科技、便携式树木直径的测量仪器。本文阐述了此仪器的测量原理及系统结构,并对定高树径测量仪的硬件结构和工作原理分别进行了说明。

高树：远见者远行

我们很重,只有到时间拉不动的时候,我们才美丽的叹息,才倚着思想的小楼尽情发愁——摘自高树小说《拒绝辩护》

传宣簪笔侍承明:试论高树《金銮琐记》

晚清民国诗人高树《金銮琐记》以纪事诗记述了清末政情及宫廷情况,包括其任清政府军机章京时的所见闻,其中包括戊戌变法、义和团运动,以及袁世凯的活动等时事诗;抒发人际遭遇的遣怀诗、书画作品等兴致诗,记述当时的社会风情、人文活动,史料与文学价值较高。

高树生卒年考

高树作为晚清民国诗人、官员、戏曲作家,有大量文学作品留世,且具有较大的文学与史学价值,但其生平和创作一直为研究者所忽视。生卒年是研究高树文学创作的基本前提,然而一直以来,或说法不一,或语焉不详,并无确切及一致记载。该文通过查找、爬梳高树诗歌自注、同时代作家诗文集及地方志、家谱等相关文献,可以考知高树生于1849年八月十五日亥时(公历10月1日)。

Research on High-Velocity Impact Damage Monitoring Method of CFRP Based on Guided Wave

Carbon fiber-reinforced polymer(CFRP)is widely used in aerospace applications.This kind of material may face the threat of high-velocity impact in the process of dedicated service,and the relevant research mainly considers the impact resistance of the material,and lacks the high-velocity impact damage monitoring research of CFRP.To solve this problem,a real high-velocity impact damage experiment and structural health monitoring(SHM)method of CFRP plate based on piezoelectric guided wave is proposed.The results show that CFRP has obvious perforation damage and fiber breakage when high-velocity impact occurs.It is also proved that guided wave SHM technology can be effectively used in the monitoring of such damage,and the damage can be reflected by quantifying the signal changes and damage index(DI).It provides a reference for further research on guided wave structure monitoring of high/hyper-velocity impact damage of CFRP.

不同品种的杜仲叶体外抗氧化性研究

实验选取了高树和矮树两种杜仲品种,设计了抗1,1-二苯基-2-苦肼基(DPPH)自由基、羟基自由基、超氧阴离子、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基(ABTS^(+))清除以及总还原力的测定实验,着重研究了不同储存时间、储存方法、加工方式、制备方式对杜仲叶抗氧化能力的影响。结果表明,相对于采摘时间、加工方式、制备方式等因素,杜仲叶的品种更影响抗氧化能力,矮树的抗自由基能力多强于高树。这为研究杜仲茶原料科学拼配及标准化、杜仲产品的深加工提供了技术支撑及新思路。

Effect of Continuous Cropping on Leaf Nutrient and Growth of Different Species of Poplar Plantation

[Objective]The paper was to study the effect of continuous cropping on leaf nutrient and growth of different species of poplar plantation.[Method] The tree height,diameter at breast height（DBH）,volume of different generations and species of poplar plantation in poplar production area in northern Jiangsu Province,as well as TN,TP and TK content in their leaves were determined through field investigation and indoor experiment.[Result] With the increasing generation,the changes of TN,TP and TK content in the leaves of different ages of two varieties of forest stand were also different.However,with the increasing tree ages,TN,TP and TK contents in leaves showed decrease trend.With the increasing generation,the changes of tree height of I-69 and I-72 poplar showed the similar trend.The effect of increasing generation on tree height of 7-year-old and 10-year-old poplar was relatively stable,showing decrease trend from generation to generation.The tree height of 4-year-old poplar first increased and then decreased with the increasing generation.The changes of DBH along with different generations were basically consistent with the changes of tree height.With the increasing cultivation generations,the volume of 7-year-old and 10-year-old poplar decreased with significant difference.[Conclusion ] The result provided basis for rational fertilization and management of poplar plantation,improvement of productivity of poplar plantation,and achievement of sustainable management of plantation.

Determination of contents of 10-Hydroxycamptothecin in Camptotheca acuminata by high-performance liquid chromatogram

The determination method of 10-hydroxycamptothecin in Camptotheca acuminata fruits by high-performance liquid chromatogram (HPLC) was studied. The HPLC analysis was performed on a HIQ sil C18(4.6×250 mm) column with mobile phase of acetonitrilewater (3:7, V:V), flow rate 1 mLmin-1 and UV detective wavelength 266 nm. Extracting 10-hydroxycamptothecin by ultrasonic method from fruits of C. acuminata to prepare samples for analysis was systematically discussed. The optimal extraction condition was carried out by 60% alcohol solution at 60℃ for 50 minutes.

Breeding and Characterization of Yunchachunhao, a New Tea Variety with Full Aroma and High Quality

[Objective] The aim of this study was to select a tea variety with full aro- ma and high quality. [Method] The quality of Yunchachunhao was identified using Yunkang 10 and Fudingdabaicha as the controls in variety comparison test, using Yunkang 10 and Dangdidayezhong as the controls in reginal test. [Result] In variety comparison test, the yield of Yunchachunhao was 39.3% more than that of Yunkang 10, and 29.6% more than that of Fudingdabaicha. In reginal test, the yield of Yun- chachunhao was 2.5% （Baoshan） and 10.2% （Lancang） more than that of Yunkang 10. The roast-dried green tea of Yunchachunhao was characterized by flowery fla- vor, good taste and bright green infused leaves. It got the total scores of 91.13 in quality identification, which was higher than that of Fudingdabaicha （90.75） and Yunkang 10 （88.85）. The steam fixed green tea of Yunchachunhao contained 28.0% polyphenols and 2.7% amino acids; and the ratio of polyphenols to amino acids was 10.4, lower than that of Fudingdabaicha （10.6） and Yunkang 10 （17.3）. Yun- chachunhao had better resistance to drought and blister blight than Yunkang 10. [Conclusion] The survival rates of cuttings and transplanted seedlings of Yun- chachunhao are higher than that of control varieties, suggesting it has better adapt- ability and can be promoted in the large-leaf tea planting area of Yunnan Province.

Analysis of Application of Superabsorbent Polymer in Sandy Soils

[Objective]The aim was to research water retention of superabsorbent polymer （SAP） in sandy soils. [Method] With soils from an ancient course in the Yel ow River as materials, acrylate SAP was taken as water retention materials to conduct the test. [Result] With 0.2%-0.4% SAP added, dewatering period of sandy soil extended after irrigation; the period of water retention quantity over 10% last for 42 d, which was significantly more than that of the control group in 16 d （saturated water reducing to 10%）; water retention effect proved better at the 2nd irrigation without soil stirring compared with the 1st irrigation. [Conclusion] The mixture of SAP with sandy soils promotes water retention in soils.

Research on Crown Grafting of Seedling Cashew Trees

Objective] The study was aimed to improve the yield and quality of cashew. [Method] Through the comparative analysis, the survival rate, new germi-nation and yield characteristics of the cutting stems with different heights by stem grafting and bark grafting. [Result] The best cutting stem height of cashew should be in 100-150 cm, when the number of new germinated shoots was 85.5-87.7. Stem grafting could significantly improve the survival rate of the shooting branch （92.7%）, growth condition of which was better than that of bark grafting, and it also had no clip cortex. The yield of stem grafting was significantly higher than that of bark grafting, and it could reach up to 18.1 kg/plant in 4 years. The branches from stem grafting had significantly better ability in resisting the wind damage than those from bark grafting. [Conclusion] The study provided references for the crown grafting of cashew planting in China.

Establishment of a Mathematical Model for Mulberry Balanced Fertilization in Hilly Areas of Sichuan Province

In order to increase mulberry leaf yield and production efficiency in hilly areas of Sichuan Province through scientific fertilization,＂3414＂,a quadratic regression design for modern fertilization,was employed to simulate the regression relationship between mulberry leaf yield and major fertilizer elements including nitrogen（N）,phosphor（P） and potassium（K） among which mulberry leaf yield was the objective function and N,P and K were the regulatory factors to fertilization level.As a result,7 types of ternary,binary and monadic quadratic fertilizer effect functions of N,P and K fertilizer elements were established.The weight of fertilizer factors in the optimal mulberry leaf yield obtained by various types of functions was in decreasing order of KPKPNPKNKNNP,and the weight of fertilizer factors in the ratio of leaf yield to input was in decreasing order of K〉PK〉NPK〉P〉NK〉NP〉N.To be specific,the ternary quadratic fertilizer effect function（Y=20 313＋0.25X1＋43.48 X2＋34.47X3-0.01X1^2-0.14X2^2-0.06X3^2＋0.05X1X2＋0.02X1X3-0.07X2X3,in which X1,X2 and X3 indicate the fertilizer factors N,P and K,respectively） was a model containing all factors,thereby being a recommended fertilization program for mulberry field.Single factor effect analysis indicated that K fertilizer had the highest influence on mulberry leaf yield and ratio of leaf yield to input.Therefore,among all the simulation functions,the monadic quadratic fertilizer effect function of K fertilizer factor（Y=25 002.27＋44.728X3-0.084X32） leads to the optimal leaf yield and maximum ratio of leaf yield to input.By using the optimal fertilization program with 600 kg/hm2 N,210 kg/hm2 P2O5 and 254.33 kg/hm2 K2O,the optimal economic leaf yield obtained is 30 945.00 kg/hm2,the cost of fertilizer input is 4 403.65 yuan/hm2,and the ratio of leaf yield to input is as high as 17.57.After verification by production practices,the recommended fertilization indicators in the optimal mulberry fertilization program can provide reference for the construction of high-yield mulberry field in hilly areas of Sichuan Province.

Effects of climate factors on the height increment of poplar protec-tion forest in the riverbank field

Based on the data of stand investigation and stem analysis, the effects of climate factors on the poplar protection forest increment in the riverbank field of the Dalinghe and Xiaolinghe rivers of Liaoning Province, China were studied by step-wise regression procedure and grey system theories and methods. A regression model reflecting the correlation between the height increment of poplar protection forest and climatic factors was developed. The order of grey relevance for the effect of climatic factors on the height increment of poplar protection forest is: light>water>heat, and it could be interpreted that the poplar increment was mainly influenced by light factor, water factor, and heat factor. This result will provide scientific basis for the in-tensive cultivation and regeneration of the poplar protection forest in riverbank field in similar regions in China.

Genetic diversity and matrilineal structure in Chinese tree shrews inhabiting Kunming,China

Due to their special phylogenetic position in the Euarchontoglires and close affinity to primates,tree shrews have been proposed as an alternative experimental animal to primates in biomedical research.However,the population genetic structure of tree shrews has largely remained unknown and this has hindered the development of tree shrew breeding and selection.Here we sampled 80 Chinese tree shrews（Tupaia belangeri chinensis） in Kunming,China,and analyzed partial mtDNA control region sequence variation.Based on our samples and two published sequences from northern tree shrews（T.belangeri）,we identified 29 substitutions in the mtDNA control region fragment（～ 604 bp） across 82 individuals and defined 13 haplotypes.Seventeen samples were selected for sequencing of the cytochrome b（Cyt b;1134 bp） gene based on control region sequence variation and were analyzed in combination with 34 published sequences to solidify the phylogenetic pattern obtained from control region data.Overall,tree shrews from Kunming have high genetic diversity and present a remarkable long genetic distance to the two reported northern tree shrews outside China.Our results provide some caution when using tree shrews to establish animal models because of this apparent genetic difference.In addition,the high genetic diversity of Chinese tree shrews inhabiting Kunming suggests that systematic genetic investigations should be conducted before establishing an inbred strain for medical and biological research.

The effect of exogenous sugar solution and high concentration of CO_2 on the contents of sugar and protein of Betula platyphylla leaves

The content of total sugar, sucrose, fructose and protein in the leaves of3-yr.-old Betula platyphylla was measured after the treatment by three exogenous sugar solutions(sucrose, fructose, glucose) and three high concentrations of CO_2 (700, 1 400, 2 100 μL/L·L^(-1))for about a month in 1998. The results showed that spraying three exogenous sugar solutionsincreased markedly the content of sugar and protein of leaves under 700 μL·L^(-1) and 1 400μL·L^(-1) CO_2 The effect of spraying exogenous sucrose solution was the best among the threeexogenous sugars. The treatment of spraying exogenous sugar solution and 2 100 μL·L^(-1) CO_2constrained the accumulation of total sugar and protein of leaves. There was no difference inprotein content of leaves when spraying glucose and fructose solutions under 700 μL·L^(-1) and 1400 μL·L^(-1) CO_2. The treatment of 2 100 μL·L^(-1) CO_2 concentration significantly increasedthe contents of total sugar, sucrose, fructose, and protein of leaves compared with that of the 700μL·L^(-1) and 1 400 μL·L^(-1) CO_2 except the plants spraying fructose solution. There waspositive correlation between the content of sugar of leaves and CO_2 concentration when sprayingsame exogenous sugar solution.

Optimization for Purification and Characterization of Recombinant Hirudin Ⅲ from E. coli

Aim To optimize purification conditions of recombinant hirudin 3 in thefermentation broth and characterize the product. Methods Reambinant hirudin 3 was isolated andpurified from the fermentation broth by three column chromatography steps with macroporous resin,DEAE cellulose DES2 and preparative RP-HPLC, respectively, and the optimal conditions were obtained.Purity of the product was determined by SDS-PAGE and analytical RP-HPLC. The molecular weight wasdetermined by mass spec-trometry. The structure of the product was analyzed by peptide map.ResultsThe product with purity of 95.4786% was obtained after three purification steps in the optimumconditions with a total yield of 39%. The molecular weight of the product was 6 913.32 ± 6.55 Da,coincident to the theoretical molecular weight of r-hirudin 3. The structure of the product wascoincident to r-hirudin 3 either. Conclusion The optimized purification steps can be successfullyemployed for purification of r-hirudin 3 from E. coli using batch-type approaches. The productobtained with high purity was confirmed to be r-hirudin 3.

Analysis of Codon Usage Between Different Poplar Species

Codon usage is the selective and nonrandom use of synonymous codons to encode amino acids in genes for proteins. The analysis of codon usage may improve the understanding of cocion preferences between different species and allow to rebuild the codons of exogenous genes to increase the expression efficiency of exogenous genes, Here, codon DNA sequence （CDS） of four poplar species, including Populus tremuloides Michx., P. tomentosa Carr., P. deltoides Marsh., and P. trichocarpa Torr. ＆ Gray., is used to analyze the relative frequency of synonymous codon （RFSC）. High-frequency codons are selected by high-frequency （HF） codon analysis. The results indicate that the codon usage is common for all four poplar species and the codon preference is quite similar among the four poplar species. However, CCT encoding for Pro, and ACT coding for Thr are the preferred codons in P. tremuloides and P. tomentosa, whereas CCA coding for Pro, and ACA coding for Thr are preferred in P. deltoides and P. trichocarpa The codons such as TGC coding for Cys, TTC coding for Phe, and AAG coding for Lys, are preferred in the poplar species except P trichocarpa. GAG coding for Glu is preferred only in P deltoides, while the other three poplar species prefer to use GAA. The commonness of preferred codon allows exogenous gene designed by the preferred cocion of one of the different poplar species to be used in other poplar species.

Responses of some landscape trees to the drought and high temperature events during 2006 and 2007 in Yamaguchi,Japan

Extreme weather events were analyzed based on the meteorological data from the year of 1967 to 2007 for Yamaguchi, Japan. The responses from landscape trees were also investigated mainly by the analysis of image pixel and spectral reflectance. Results show that after the dry, hot and windy summer in 2007, many landscape trees in Yamaguchi City tended to respond the extreme weather events by reducing their leaf surface area and receiving less radiation energy. Premature leaf discoloration or defoliation appeared on some landscape tree species and leaf necrosis occurred on tip and margin of many Kousa dogwood (Cornus kousa) trees at unfavorable sites. Described by image pixel analysis method, the leaf necrotic area percentage (LNAP) of sampled dogwood trees averaged 41.6% and the sampled Sasanqua camellia (Camelia sasanqua) tree also showed fewer flowers in flower season of 2007 than that in 2006. By differential analysis of partial discolored crown, it presented a logistic differential equation of crown color for sweet gum (Liquidambar styraciflua) trees. It suggested that the persistent higher temperature and lower precipitation could be injurious to the sensitive landscape trees at poor sites, even in relative humid area like Yamaguchi.