[Objective] This study aimed to measure the hesperidin content in citrus peel by high performance liquid chromatography, to provide a scientific basis for quality control and identification. [Method] The hesperidin wa...[Objective] This study aimed to measure the hesperidin content in citrus peel by high performance liquid chromatography, to provide a scientific basis for quality control and identification. [Method] The hesperidin was extracted with alkaline solution at 70 ~C and pH 6-7, and the purity of hesperidin was determined by HPLC. [Result] The formula for the regression line was Y=466,097Xq3.415 0 （r=0.999 6）, identify- ing the relationship between hesperidin concentration and peak area, and the linear range was 0.2-1.4μg. The hesperidin solution was stable within 24 h at room temperature. The average recovery rate of hesperidin was 98.41%. [Conclusion] The HPLC method is rapid, simple, and with good linear relationship, can be used for routine analysis of hesperidin.展开更多
文摘[Objective] This study aimed to measure the hesperidin content in citrus peel by high performance liquid chromatography, to provide a scientific basis for quality control and identification. [Method] The hesperidin was extracted with alkaline solution at 70 ~C and pH 6-7, and the purity of hesperidin was determined by HPLC. [Result] The formula for the regression line was Y=466,097Xq3.415 0 （r=0.999 6）, identify- ing the relationship between hesperidin concentration and peak area, and the linear range was 0.2-1.4μg. The hesperidin solution was stable within 24 h at room temperature. The average recovery rate of hesperidin was 98.41%. [Conclusion] The HPLC method is rapid, simple, and with good linear relationship, can be used for routine analysis of hesperidin.
