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刺五加中紫丁香苷的制备及其含量测定 被引量:6
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作者 袁晔蓉 李强 《辽宁中医杂志》 CAS 北大核心 2007年第6期809-811,共3页
目的:首先对刺五加中有效成分紫丁香苷进行了提取分离和结构鉴定,自制定量用的紫丁香苷标准品。方法:建立了刺五加中紫丁香苷的反相高效液相色谱定量法:以甲醇-乙腈-水-冰醋酸(14∶1∶85∶1,v∶v∶v∶v)为流动相,检测波长270nm。... 目的:首先对刺五加中有效成分紫丁香苷进行了提取分离和结构鉴定,自制定量用的紫丁香苷标准品。方法:建立了刺五加中紫丁香苷的反相高效液相色谱定量法:以甲醇-乙腈-水-冰醋酸(14∶1∶85∶1,v∶v∶v∶v)为流动相,检测波长270nm。结果:紫丁香苷在1.24~31.0μg/mL质量浓度内线性关系良好(r=0.9997),平均回收率为100.1%(RSD 2.2%)。结论:该法简便快速,结果准确可靠。该方法可作为刺五加质量控制的手段之一。 展开更多
关键词 刺五加 紫丁香苷 高液液相色谱法
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Stability of Baicalin Aqueous Solution by Validated RP-HPLC 被引量:6
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作者 仇峰 唐星 +1 位作者 何仲贵 李好枝 《Journal of Chinese Pharmaceutical Sciences》 CAS 2004年第2期134-137,共4页
Aim In the present study a RP-HPLC method was developed and validated toinvestigate the stability of baicalin aqueous solution. Methods The influences of temperature and pHon the stability of baicalin aqueous solution... Aim In the present study a RP-HPLC method was developed and validated toinvestigate the stability of baicalin aqueous solution. Methods The influences of temperature and pHon the stability of baicalin aqueous solution were investigated by classic homoiothermicacceleration test, and the pH for the most stable solution was determined. Results The time whenbaicalin suffered 10% loss was found to be 18.1 h, and the degradation activation energy of baicalinwas 79.1 kJ·moL^(-1) . The pH at which baicalin is most stable is 4.28. Conclusion The temperatureshould be kept at a lower level and the pH should be adjusted to near that for the most stablesolution in the production of baicalin preparations. 展开更多
关键词 RP-HPLC BAICALIN STABILITY TEMPERATURE PH
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A HPLC Method for the Determination of Salmon Calcitonin in Injection by Gradient Elution 被引量:1
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作者 陈小平 郭庆东 张强 《Journal of Chinese Pharmaceutical Sciences》 CAS 2003年第2期114-116,共3页
It was concluded that the described HPLC method could be used for the assayof salmon calcitonin in injection, as it offers qualified selectivity, accuracy and precision ofanalysis.
关键词 HPLC ASSAY salmon calcitonin injection external standard method
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Determination of Candicidin/FR-008 and Related Components in Fermentation Broth by RP-HPLC
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作者 毛相朝 沈亚领 +2 位作者 魏东芝 陈实 邓子新 《Journal of Chinese Pharmaceutical Sciences》 CAS 2005年第2期115-118,共4页
Aim A liquid chromatographic method for the determination ofcandicidin/FR-008 and related components in fermentation broth has been developed. Methods Therewere four major components in the candicidin/FR-008 complex, ... Aim A liquid chromatographic method for the determination ofcandicidin/FR-008 and related components in fermentation broth has been developed. Methods Therewere four major components in the candicidin/FR-008 complex, which were separated by HPLC under thefollowing conditions: SB-C8 column (4.6 mm x 250 mm, 5 μm) was used, the mobile phase consisted ofacetonitrileam-monium acteate (20 mmol·L^(-1) , pH 4.0) (40:60, V/V) , with a flow rate of 1 .0mL·min^(-1) , the UV detection wavelength was 380 nm, and the whole process was performed at 25℃ .Results The linearity was obtained in the range of 6.25 - 500 μg· mL^(-1) candicidin/FR-008 withthe regression equation of Y = 20 461 x + 30 748 and the correlation coefficient of 0.999 1. Theinstrument precision was 1.84% and the method precision was 3.8%. Conclusion This method isaccurate, rapid and simple; it can be used for determination of candicidin/FR-008 and relatedcomponents in fermentation broth. 展开更多
关键词 candicidin/FR-008 HPLC fermentation broth METABOLITES
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小儿热速清糖浆中山银花检查方法研究
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作者 代喜国 《中文科技期刊数据库(文摘版)医药卫生》 2018年第12期220-221,共2页
建立小儿热速清糖浆中山银花检查方法,利用此法甄别小儿热速清糖浆违规投料问题,确保患者用药的有效性。方法:采用Agilent Zorbax Eclipse XDB-C18(250mm×4.6mm i.d.,5μm)色谱柱;以乙腈为流动相A,水为流动相B,进行梯度洗脱;柱温... 建立小儿热速清糖浆中山银花检查方法,利用此法甄别小儿热速清糖浆违规投料问题,确保患者用药的有效性。方法:采用Agilent Zorbax Eclipse XDB-C18(250mm×4.6mm i.d.,5μm)色谱柱;以乙腈为流动相A,水为流动相B,进行梯度洗脱;柱温为32℃;流速为每分钟1.0ml;蒸发光散射检测器。结果:该方法检测限为48ng,测定条件有小的变动能满足系统适用性试验要求,能够确保方法的可靠性。结论:本法专属性强,可用于制剂产品的质量控制及药品监管机构甄别山银花与金银花投料问题的补充检验方法。 展开更多
关键词 高液液相色谱法 金银花 山银花 灰毡毛忍冬皂苷乙 、灰毡毛忍冬皂苷甲 川续断皂苷乙
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Fucoxanthin Content of Five Species Brown Seaweed from Talango District, Madura Island 被引量:1
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作者 K. Zailanie H. Purnomo 《Journal of Agricultural Science and Technology(A)》 2011年第8期1103-1105,共3页
Fucoxanthin content of five brown seaweed species of East Java collected from Talango district (Madura sea-Jawa sea) namely Padina australis, Turbinaria conoides, Sargassum cinereum, Sargassum filipendula and Sargas... Fucoxanthin content of five brown seaweed species of East Java collected from Talango district (Madura sea-Jawa sea) namely Padina australis, Turbinaria conoides, Sargassum cinereum, Sargassum filipendula and Sargassum echinocarpum were determined and samples were collected using Global Positioning System (GPS). Fucoxanthin from those samples was determined using Thin Layer Chromatography (TLC), High Pressure Liquid Chromatography (HPLC) and Spectrophotometer UV-Vis and the results were then calculated using Seely equation. The pigments separation using HPLC gave a better result compared to TLC, and beside fucoxanthin otlher pigments namely chlorophyll and β carotene were also identified in these samples. Four points of sampling location were chosen using GPS and spot with coordinate positions observed were 113.94444° EL-7.08795° SA; 113.94231° EL-7.08913° SA. The results showed that fucoxanthin content were as follow: Padina australis 0.2674± 0.0046 mg/g; Turbinaria conoides 0.2134± 0.0100 mg/g; Sargassum filipendula 0.1957 ± 0.0173 mg/g; Sargassum cinereum 0.1640 ± 0.0092 mg/g and Sargassum echinocarpum 0.1576 ± 0.0001 mg/g. It can be concluded that Padina australis and Turbinaria conoides contain the most amount of fucoxanthin and it is possible to be cultivated as edible brown seaweed fucoxanthin resources. 展开更多
关键词 Fucoxan thin brown seaweed Madura Island
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Persistence of malachite green and leucomalachite green in perch (Lateolabrax japonicus) 被引量:1
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作者 谭志军 邢丽红 +4 位作者 郭萌萌 王洪艳 江艳华 李兆新 翟毓秀 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2011年第3期647-655,共9页
The persistence of malachite green (MG), and its metabolite leucomalachite green (LMG), in fish tissues is still unclear, leading to many trade disputes. In this research, we established and evaluated an HPLC method t... The persistence of malachite green (MG), and its metabolite leucomalachite green (LMG), in fish tissues is still unclear, leading to many trade disputes. In this research, we established and evaluated an HPLC method that could detect MG and LMG simultaneously, and then investigated the persistence of these two toxins in the tissues of juvenile perch (Lateolabrax japonicus) post sub-chronic MG exposure at 1.0 mg/L. Exposure lasted for 2 h everyday and was repeated six times. The perch were then placed in MG-free seawater for 100 d to eliminate the toxins. Results show that MG accumulated in the tissues, including the gills, liver, muscle, blood and viscera, and then was metabolized rapidly to LMG. The concentrations of these two toxins increased significantly with the accumulation process. In general, the highest concentrations of MG and LMG in all tissue exceeded 1 000 μg/kg, except for MG in the muscle. The order of accumulation levels (highest to lowest) of MG was gill>blood>liver>viscera>muscle, while that of LMG was liver>blood>gill>viscera>muscle. High levels of MG or LMG could persist for several hours but decreased rapidly during the elimination process. The concentration of LMG was much higher than that of MG during the experiment, especially in the gill, liver and blood. Therefore, the three tissues play important roles in toxin accumulation, biotransformation, and elimination. Although the MG and LMG concentrations in muscle were much lower than in other tissues, the content still exceeded the European minimum required performance limit (MRPL), even after 2 400 h (100 d) of elimination. This demonstrates that it is extremely difficult to eliminate MG and LMG from tissues of perch, and therefore use of these toxins is of concern to public health. 展开更多
关键词 PERSISTENCE RESIDUES malachite green leucomalachite green PERCH
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Economic and Qualitative Traits of Italian Alps Saffron 被引量:1
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作者 Alessandra MANZO Sara PANSERI +1 位作者 Danilo BERTONI Annamaria GIORGI 《Journal of Mountain Science》 SCIE CSCD 2015年第6期1542-1550,共9页
Saffron, obtained from the flower stigmas of Crocus sativus L., is one of the most expensive food spices. The introduction of saffron in alpine areas could help to broaden and diversify the activities of mountain mult... Saffron, obtained from the flower stigmas of Crocus sativus L., is one of the most expensive food spices. The introduction of saffron in alpine areas could help to broaden and diversify the activities of mountain multifunctional farms, with a positive impact on economy and land management. According to ISO 3632(2010/2011), saffron can be classified into three categories of quality(I, II, III) depending on the concentration of the three main metabolites responsible for its characteristic colour, flavor and aroma: Crocin, Picrocrocin and Safranal. This study represents the first investigation of the quality of saffron produced in the Italian Alps evaluated with spectrophotometry, HPLC, solid-phase microextraction(SPME), and gas chromatographic analysis combined with mass spectrometry(GC/MS). The experiments used Crocus sativus stigmas produced in 2012-2013 in different areas of the Central Italian Alps were located at an altitude between 720 and 1200 m a.s.l.. Results obtained were compared to commercial saffron. The analyses confirmed that all samples can be classified in the first quality category according to the ISO classification. This high quality is also confirmed by HPLC analysis. Moreover, the SPME-GC/MS analysis identified some differences in the aromatic profile of saffron samples, in particular regarding safranal concentration. A preliminary assessment of the economic viability of high quality saffron production for local markets was also performed. Our study provides valid information regarding the quality and economic sustainability of saffron production in the alpine area confirming this crop as a good candidate for a new source of income for multifunctional farms in mountain areas. 展开更多
关键词 Crocus sativus L. Alps ISO 3632 UV– Vis spectrophotometry SPME-GC/MS HPLC
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Extraction,Isolation and Structural Characterization of Polysaccharides from a Red Alga Gloiopeltis furcata 被引量:3
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作者 YU Guangli HU Yannan YANG Bo ZHAO Xia WANG Peipei JI Guoli WU Jiandong GUAN Huashi 《Journal of Ocean University of China》 SCIE CAS 2010年第2期193-197,共5页
Three kinds of polysaccharides: GFW, GFH and GFA, were sequentially extracted from a red alga Gloiopeltisfurcata with 25℃ and 85℃ water, and 60℃ 4% NaOH water solution. Based on the defatted alga, the yields of th... Three kinds of polysaccharides: GFW, GFH and GFA, were sequentially extracted from a red alga Gloiopeltisfurcata with 25℃ and 85℃ water, and 60℃ 4% NaOH water solution. Based on the defatted alga, the yields of the polysaccharide were 57.9%, 2.5% and 2.6%, respectively. Their monosaccharide compositions, average molecular weights and structural characters were determined by gas chromatography (GC), high performance liquid chromatography (HPLC), fourier transform infrared spectroscopy (FTIR) or ^13C-NMR spectroscopy. The results showed that GFW, GFH and GFA were all composed of D-galactose (Gal) and 3,6-anhydro-L-galactose (AnG), and particularly GFA also contained xylose (Xyl). The average molecular weights of GFW, GFH and GFA were 22.6 kD, 26.5 kD and 49.8 kD, respectively, with the respective sulfate content 31.2%, 25.1% and 22.7%. The data of FTIR and ^13C-NMR confirmed the sulfate ester location at C6 ofgalactose. It is concluded that all the three polysaecharides extracted from Gloiopeltisfurcata were sulfated galactans, two being sulfated-agarose, and one being xylose-containing sulfated galactan. 展开更多
关键词 Gloiopeltisfurcata sulfated galactan EXTRACTION structural characterization
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Changes of Metabolites and Macro- and Micro-elements in Hungarian Potatoes under Organic and Conventional Farming
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作者 Rita Tomoskozi-Farkas Nora Adanyi +6 位作者 Magdolna Gasztonyi-Nagy Maria Berki Viola Horvath Tibor Renkecz Kinga Simon Zoltan Fabulya Zsolt Polgar 《Journal of Agricultural Science and Technology(B)》 2016年第2期83-92,共10页
To study the effect of cultivation system (conventional and organic) on potato tuber components, 42 parameters (anti-nutritives, micro- and macro-elements and 23 metabolites) were studied in three multi-resistant ... To study the effect of cultivation system (conventional and organic) on potato tuber components, 42 parameters (anti-nutritives, micro- and macro-elements and 23 metabolites) were studied in three multi-resistant Hungarian potato varieties for three years in 2007-2009. Discriminant analysis of data proved that all investigated factors (farming technology, genotype and season/year) had significant effect on metabolites. Reversed phase high performance liquid chromatographic (HPLC-DAD, HPLC-MS) and elemental analysis methods have been adapted to quantify the major components, i.e., steroidal alkaloids, nitrite, nitrate, sugars, amino acids and micro-elements, in potato tubers. The absolute amount and changes of tuber components were influenced differentially by the technology, genotype and season in a complex manner. Any examined component, except nitrate content, was found to be significantly higher or lower consistently in relation to the production practice (organic or conventional) during the three years trials. Under the examined circumstances, no consequent positive effect of organic fanning on the total amount of anti-nutritive components, vitamins or micro- and macro-elements of potato tubers could be proven. 展开更多
关键词 POTATO organic farming conventional fanning metabolomics macro- and micro-elements.
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Ultrafiltration of Shiitake Polyphenol with Ultrasound Enhancement
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作者 Defang SHI Hong GAO +3 位作者 Xiuzhi FAN De YANG Shujing XUE Wei CHENG 《Agricultural Science & Technology》 CAS 2015年第10期2243-2246,共4页
Ultrafiltration (UF) experiments were subjected to the concentration of Shi- itake polyphenol(SP) with ultrasound (US) enhancement, by which the effects of the main parameters such as ultrasonic power, transmemb... Ultrafiltration (UF) experiments were subjected to the concentration of Shi- itake polyphenol(SP) with ultrasound (US) enhancement, by which the effects of the main parameters such as ultrasonic power, transmembrane pressure, temperature and axial flow-rate on the membrane fluxes were analyzed, and then the SP of the ultrafiltrate after follow-up resin adsorption by HPLC was investigated. The results in- dicated that US could significantly enhance the membrane fluxes. The optimal con- ditions obtained was as follows: 0.60 W/cm2 US power, axial flow-rate of 80 L/h, operation temperature 30 ~C, transmembrane pressure 0.10 MPa. US increased the absorption capacity of the absorbent resin during the following stage. The HPLC analysis also showed that catechins were purified to some extent as the ultrafiltrate was treated by US and macroporous resin, indicating the technology of US en- hancement coupled with UF showed the potential for concentration and purification of SP by absorbent resins. 展开更多
关键词 SHIITAKE ULTRAFILTRATION ULTRASOUND
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Removal of Pharmaceutically Active Compounds in Sequencing Batch Reactor
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作者 Beata Kamifiska Koleta Majewska Anna Skwierawska Natalia Lukasik Katarzyna Koztowska-Tylingo , 《Journal of Environmental Science and Engineering(A)》 2015年第9期484-489,共6页
Biological treatment efficiency of six pharmaceutical compounds (acetazolamide, metronidazole, opipramol, piracetam, salicylamide and tinidazole) was evaluated using lab-scale Sequencing Batch Reactor (SBR). Compa... Biological treatment efficiency of six pharmaceutical compounds (acetazolamide, metronidazole, opipramol, piracetam, salicylamide and tinidazole) was evaluated using lab-scale Sequencing Batch Reactor (SBR). Comparative biological degradation processes of two types of activated sludge from municipal and pharmaceutical industry sewage treatment plants were examined. Three different organic loadings (0.05 g COD/g MLSS.d, 0.1 g COD/g MLSS.d and 0.2 g COD/g MLSS-d) and reaction time on the efficiency of Active Pharmaceutical Ingredient (API) decomposition were examined. Chemical oxygen demand, non-purgeable organic carbon as well as ammonium nitrogen contents were monitored by standard methods. Percentage of API decomposition was analysed by High Performance Liquid Chromatography (HPLC). The overall API removal efficiency was strictly dependent on the type of activated sludge origin. The main biodegradation products were identified using HPLC-MS,1H NMR and 13C NMR methods as e.g. ({4-[3-(5H-dibenzo[b,f]azepin-5-yl]piperazin-l-yl}methanamine) and (2-amino-1,3,4-thiadiazol-5-sulfonamide) for opipramol and acetazolamide respectively. 展开更多
关键词 Active pharmaceutical ingredients sequencing batch reactor biodegradation.
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Production of Aflatoxins from Aspergillus flavus in Liquid Medium 被引量:1
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作者 Maria Edite Bezerra da Rocha Francisco das Chagas Oliveira Freire +4 位作者 Icaro Gusmao Pinto Vieira Jose Mariados Santos Filho Fabio Erlan Feitosa Maia Maria Izabel Florindo Guedes Davide Rondina 《Journal of Life Sciences》 2013年第4期377-381,共5页
Aflatoxins are produced mainly by Aspergillus flavus and Aspergillus parasiticus, and can be found in many grains such as peanuts, soybeans and com. This study aimed to qualitatively and quantitatively evaluate the pr... Aflatoxins are produced mainly by Aspergillus flavus and Aspergillus parasiticus, and can be found in many grains such as peanuts, soybeans and com. This study aimed to qualitatively and quantitatively evaluate the production of aflatoxin in liquid media using strains of Aspergillus flavus obtained from peanuts marketed in the city of Fortaleza, CEo Strains of Aspergillus flavus were inoculated into a liquid medium malt extract and after 2 days inoculated into a second medium containing sucrose 5%, MgSO4·7H20 0.1%, KH2PO4 1%, ZnSO4·7H2O 0.0176 g, and cultured for 3 more days. The media were kept at room temperature ranging from 24°C to 32 °C with agitation of 130 rpm and aeration of 4.17 Llmin. Qualitative analysis was performed by thin layer chromatography and quantitatively by high performance liquid chromatography with fluorescence detection, demonstrating the production of aflatoxin B I (588 mg/L) and B2 (929 mg/L). 展开更多
关键词 PEANUT FUNGI micotoxins chromatography.
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Degradation of Antibiotics in Aqueous Solution by Photocatalytic Process: Comparing the Efficiency in the Use of ZnO or TiO2
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作者 Barbara Ambrosetti Luigi Campanella Raffaella Palmisano 《Journal of Environmental Science and Engineering(A)》 2015年第6期273-281,共9页
The study examined the photodegradative efficiency of ZnO and TiO2 in degradation of antibiotics in aqueous matrices. Among several types of antibiotics, four antibiotics were chosen to feature the major classes of th... The study examined the photodegradative efficiency of ZnO and TiO2 in degradation of antibiotics in aqueous matrices. Among several types of antibiotics, four antibiotics were chosen to feature the major classes of these compounds: amoxicillin, erythromycin, streptomycin and ciprofloxacin. Degradation of antibiotic solutions was carried out mainly under UV-light irradiation in a set time with the presence of small quantity of zinc oxide or titanium dioxide. Solutions were analyzed with HPLC chromatography and degradation percentages were calculated from ratio between pick area associated to no degraded drug solution and degraded drug solution's pick area. Meanwhile, toxicity of antibiotics and degrading compounds were investigated using a biosensor system, consisting of Clark's electrode associated with a portion of agar medium culture containing Saccharomyces Cerevisiae yeast cells. This way, it was possible to define the oxygen that was consumed by yeast cells. Toxicity associated to antibiotics and degrading products are related to decrease of oxygen concentration in solution. It is clear that zinc oxide is slower than titanium dioxide to degrade antibiotics, but zinc oxide shows better photodegradation efficiency than titanium dioxide in spite of its small specific superficial area. 展开更多
关键词 ANTIBIOTICS DEGRADATION PHOTO-CATALYSIS ZNO TIO2 toxicity.
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Simultaneous determination of four nucleosides in Carthamus tinctorius L.and Safflower injection using high-performance liquid chromatography 被引量:1
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作者 周斐然 赵明波 屠鹏飞 《Journal of Chinese Pharmaceutical Sciences》 CAS 2009年第4期326-330,共5页
We quantitatively determined four nucleosides, including cytidine, uridine, guanosine, and adenosine, in Carthamus tinctorius L. and Safflower injection. Separation was performed on a Zorbax Eclipse XDB-18 column usin... We quantitatively determined four nucleosides, including cytidine, uridine, guanosine, and adenosine, in Carthamus tinctorius L. and Safflower injection. Separation was performed on a Zorbax Eclipse XDB-18 column using a gradient elution with mobile phases of 0.05% trifluoroacetic acid (TFA) aqueous solution (A) and methanol (B). The assay was carried out at a flow rate of 1 mL/min at 25 ℃ with detection at 260 nm. Cytidine, uridine, adenosine and guanosine showed good linearity in the ranges of4.02-503μg/mL (r2= 0.9998), 9.38-1407 μg/mL (rz = 0.9999), 80.6-8060μg/mL (r2 = 0.9999) and 2.10---630μg/mL (r2 = 0.9987) with average recoveries of 97.2%, 94.5%, 98.6% and 108.6%, respectively. The contents of cytidine, uridine, adenosine and guanosine in different Carthamus tinctorius L. and Safflower injection were significantly different. This is the first report on the quantitative determination of nucleosides in Carthamus tinctorius L. and Safflower injection. 展开更多
关键词 Carthamus tinctorius L. Safflower injection NUCLEOSIDE High-performance liquid chromatography
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Simultaneous determination of ginsenosides Rg_1,Re and Rb_1 in rat plasma by HPLC and its application to pharmacokinetic study of SHENMAI injection 被引量:2
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作者 薛清丹 王鹏 +1 位作者 康宇红 李秋红 《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2015年第7期475-481,共7页
In the present study, we developed a sensitive and efficient high performance liquid chromatography (HPLC) method for the simultaneous determination of three ginsenosides (Rg1, Re, Rb1) in rat plasma. Chromatograp... In the present study, we developed a sensitive and efficient high performance liquid chromatography (HPLC) method for the simultaneous determination of three ginsenosides (Rg1, Re, Rb1) in rat plasma. Chromatographic separation was performed on a C18 (150 min×4.6 mm) column utilizing gradient elution profile and a mobile phase consisting of (A) water and (B) acetonitrile. The calibration curve, with a great correlation coefficient greater than 0.998, was linear over the range of 1.0-30.0 μg/mL for ginsenoside Rgl, 0.5-15.0 μg/mL for ginsenoside Re, and 0.5-200.0 μg/mL for ginsenoside Rb1. The intra- and inter-day precisions for three ginsenosides (Rg1, Re, Rb1) were all less than 6.0%, and average recovery, examined at three concentration levels, ranged from 96.1% to 118.6%. The samples was stable within 24 h at 4 ℃ storage, and 30 d at -20 ℃ storage with three freeze-thaw-assay cycles. The low limits of quantification (LOQ) were 1.0, 0.5 and 0.5 μg/mL for Rg1, Re and Rb1, respectively. Taken together, the newly developed method was successfully applied to study the pharmacokinetics of ginsenoside Rg1, Re and Rb1 in rat plasma after intravenous administration of SHENMAI injection (SMI). 展开更多
关键词 Ginsenoside Rg1 RE RB1 HPLC SHENMAI injection PLASMA
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A high performance liquid chromatography method for the quantitative determination of erlotinib in the plasma of tumor bearing BALB/c nude mice and its application in a pharmacokinetic study
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作者 李汉青 陈烨 +6 位作者 李载权 邓晨辉 李良 毕姗姗 李梦瑶 周田彦 卢炜 《Journal of Chinese Pharmaceutical Sciences》 CAS 2011年第3期245-252,共8页
A new HPLC-UV method was developed and validated for the quantitative determination of epidermal growth factor receptor inhibitor erlotinib in the plasma of tumor bearing BALB/c nude mice.Erlotinib and its internal st... A new HPLC-UV method was developed and validated for the quantitative determination of epidermal growth factor receptor inhibitor erlotinib in the plasma of tumor bearing BALB/c nude mice.Erlotinib and its internal standard l-(3-((6,7-bis(2- methoxyethoxy)quinazolin-4-yl)amino)phenyl)ethanone were extracted from mice plasma samples using liquid-liquid extraction with a mixed solvent of methyl t-butyl ether and ethyl acetate(9:1,v/v).Chromatographic separation was performed on a Luna C|_(18)column(4.6 mm×250 mm,5μm)with acetonitrile:5 mM potassium phosphate buffer pH=5.2(41:59,v/v)as the mobile phase.UV detector was set at the wavelength of 345 nm,and the flow rate was 1.0 mL/min.The calibration curve was linear over the range of 20-10 000 ng/mL with acceptable intra-and inter-day precision and accuracy.The intra-day and inter-day precisions were within the range of 1.69%—5.66%,and the accuracies of intra-and inter-day assays were within the range of 105%—113%. The mean recoveries were 85.2%and 96.1%for erlotinib and IS,respectively.This method was successfully applied to a pharmacokinetic study in tumor bearing BALB/c nude mice following single oral administration at the dose of 12.5 mg/kg. The main pharmacokinetic parameters were as follows:C_(max)was 4.67μg/mL,T_(max)was 1.0 h,T_(1/2)was 2.78 h,and AUC_(0-24h)was 18.0μg/mL·h. 展开更多
关键词 ERLOTINIB HPLC-UV Liquid-liquid extraction PHARMACOKINETICS Epidermal growth factor receptor TUMOR
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Combination of dynamic hollow fiber liquid-phase microextraction with HPLC analysis for the determination of UV filters in cosmetic products 被引量:2
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作者 YANG HongYun LI HaiFang +3 位作者 Masahito Ito LIN Jin-Ming GUO GuangSheng DING MingYu 《Science China Chemistry》 SCIE EI CAS 2011年第10期1627-1634,共8页
A simple method based on hollow fiber liquid-phase microextraction (HF-LPME) followed by high performance liquid chro-matography (HPLC) analysis was successfully developed for the determination of UV filters in cosmet... A simple method based on hollow fiber liquid-phase microextraction (HF-LPME) followed by high performance liquid chro-matography (HPLC) analysis was successfully developed for the determination of UV filters in cosmetic products. A canular extractor was assembled by mounting a hollow fiber inside an external tube with a tee-connector. The organic solvent was immobilized into the fiber to form a liquid membrane as the acceptor phase. The sample was continuously injected into the extractor and the UV filters were extracted from the aqueous sample into organic acceptor phase. The main parameters affecting HF-LPME including extraction solvent, sample volume, sample flow rate, pH values and ionic strength were investigated. Toluene has been verified to be suitable as the acceptor phase. Under the optimized HF-LPME conditions, the enrichment factors of five UV filters varying from 24 to 57 were achieved. The limits of detection for the five UV filters were in the range of 1-100 μg L-1 .The relative standard deviations (RSDs) of HF-LPME and HPLC analysis were lower than 5.2%. The proposed method has been successfully applied to the analysis of the varied cosmetic products. 展开更多
关键词 hollow fiber liquid-phase microextraction UV filter high performance liquid chromatography COSMETICS
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