“高生素1号”对茶叶化学成分和茶叶品质的影响

用“高生素 1号”叶面喷施剂处理大棚覆盖的龙井 -43茶树 ,氨基酸含量比对照高 1 .5 -1 2 .9% ,儿茶素类总含量增加 4.6-1 9.5 % ;茶叶品质感官评分比对照高 2 .3 -2 .6%。

Effects of Atrazine Stress on the Growth of Pennisetum hydridum

[Objective] This study was to investigate the effect of atrazine stress on the growth of Pennisetum hydridum. [Method] Pot experiments were conducted to study the effects of atrazine stress （20, 50, 100, 200, 500 mg/kg） on plant height, biomass, root-shoot ratio and chlorophyll content of P. hydridum. [Results] Low level of atrazine stress （20 and 50 mg/kg） showed no significant effects on plant height and biomass of P. hydridum. Moderate level of atrazine stress （100 and 200 mg/kg） did not show significant effect on plant height, but did on the biomass of P. hydridum. Biomass of P. hydridum stressed by 100 and 200 mg/kg of atrazine was decreased by 34.1% and 36.4% compared with control, respectively. High level of atrazine stress （500 mg/kg） brought significant decrease in plant height（by 40.6%） and biomass（20.0%） of P. hydridum. All levels of atrazine stress showed no significant effects on root-shoot ratio and chlorophyll content of P. hydridum. [Conclusion] Pennisetum hydridum has strong tolerance to atrazine stress.

不同日粮饲喂肥育猪的效果试验

供试验猪 92头 ,均为杜长嘉三元杂种肉猪 ,随机分为 3组 ,采用天然生长肽 (高生素 )加有机铬 (烟酸铬 )作添加剂 ,配制试验 1组饲料 ;天然生长肽加中药“891”,配制试验 2组饲料 ;嘉兴某饲料公司生产的“10 1”饲料为试验 3组饲料。经 75 d饲喂试验 ,1组日增重 5 71g,料重比2 .90∶ 1,瘦肉率 5 8.32 %;2组分别为 4 87g,3.0 8∶ 1,5 5 .84 %;3组为 5 76 g,3.5 3∶ 1,5 5 .70 %。

Responses of Chlorophyll Fluorescence and Carotenoids Biosynthesis to High Light Stress in Rice Seedling Leaves at Different Leaf Position

In the present study, we investigated the changes of photosynthesis, chlorophyll fluorescence and the content of carotenoid pigments in rice (Oryza sativa L.) seedling leaves and their responses to high light. The results showed that the rate of photosynthesis, the contents of individual and total carotenoids and the pool size of xanthophyll cycle decreased with age increasing of the leaf. When the leaves were exposed to high light for 2 h, the qN of mature leaf (5th leaf) increased more significantly than that of younger (6th leaf) and older leaves (3rd and 4th leaf). Comparing with the leaves before exposure to high light, the excitation pressure on PSⅡ (1- qP ) increased by 44%, 57%, 19% and 45% in the 3rd, 4th, 5th and 6th leaf under high light, respectively. The highest content of carotenoids and the greatest conversion of violaxanthin to zeaxanthin were found in the 5th leaf, and it was consistent with the 5th leaf exhibiting the strongest resistance to high light. Our results suggested that the ability of rice leaf to resist photoinhibition is related to the level of carotenoids and the ability of carotenoids biosynthesis.

Chlorophyll Fluorescence and Membrane Lipid Peroxidation in the Flag Leaves of Different High Yield Rice Variety at Late Stage of Development Under Natural Condition

With indica ( Oryza sativa L.) hybrid Shanyou 63 as control, the hybrid rice varieties including Peiai 64S/E32, Peiai 64S/9311, X07S/Zihui 100, Guangyou 881 and japonica 9516 were used to study changes of chlorophyll content, photosynthetic response to light intensity and temperature, chlorophyll fluorescence characteristics and membrane lipid peroxidation in their flag leaves at the late stage of development under natural conditions in Nanjing. The results were as follows:. primary photochemical efficiency of PS II ( F-v / F-m), quantum yield of linear electron transport of PS II (phi(PSII)), electron transfer rate (ETR) in these rice varieties decreased with their decrease of chlorophyll content during this period. This kind of impediment to energy conversion induced the transfer of excessive energy to the reducing side of PS I, hence the accumulation of O-2(radical anion) and peroxidation of membrane lipid, and resulting in the accumulation of malondialdehyde (MDA), that is the destroys of photosynthetic pigments and membranes and the consequent, premature senescence. This phenomenon is variable conspicuously in different rice varieties. Under natural condition in Nanjing, F-v/F-m, phi(PSII), ETR and quenching coefficient ( qP) in japonica 9516 tolerant to photooxidation decreased less and the conversion capacity of light energy was stable, premature senescence was unlikely, and consequently the seed-setting rate was higher. While F-v/F-m, phi(PSII), ETR and photochemical qP in Shanyou 63 sensitive to photooxidation decreased more and therefore premature senescence was easy to happen, thus the seed-setting rate and yield were all reduced. The tolerance to photooxidation and premature senescence in other hybrids derived from typical two line or three line crossing laid in the middle. From the rice breeding for super-high-yield, on the basis of the good plant-type of current rice, considering both hybrid vigor and the prevention premature senescence, it would be a notable strategy to use japonica maternal line or maternal. lines with some japonica genotype as the sterile lines in rice breeding.

A Pre-column Derivatization HPLC Method for the De-termination of Peimine and Peiminine in Bulbus Fritillar-iae

A pre-column derivatization HPLC method for the determination of peimine(P)and peiminine(PE)in Bulbus Fritillariae has been developed. Under the derivatization conditions optimized,the calibration curve is Y1=-3.83×103+1.33 ×105X1,r=0.998 for P and Y2=-7.86 × 102+6.33 × 104X2,r=0995 for PE,where Y is the peak area and X is the weight of the alkaloid; the average recovery is 98.0%(n=5, RSD=2.1%) for P and 101.0%(n=5,RSD=4.1%)for PE,the linear range is from 0.504 μg to 3.126μg for P and from 0.520μgto 3.328μgfor PE,respectively. Results of the determination of the two alkaloids in several samples of different Fritillaria species from various parts ofthe country are presented.The results suggest that P and PE are two major chemical constituents in bulbs of different Fritillaria species,and that the method developed is generally appli-cable to the determination of the hydroxy group on aliphatic fused ring systems without steric hin-drance.

Response of seedlings of different tree species to elevated CO_2 in Changbai Mountain

Eco-physiological responses of seedlings of eight species, Pinus koraiensis, Picea koraiensis, Larix olgensis, Populus ussuriensis, Betula platyphylla, Tilia amurensis, Traxinus mandshurica and Acer mono from broadleaved/Korean pine forest, to elevated CO2 were studied by using open-top chambers under natural sunlight in Changbai Mountain, China in two growing seasons (1998-1999). Two concentrations of CO2 were designed: elevated CO2 (700 祄olmol-1) and ambient CO2 (400 祄olmol-1). The study results showed that the height growth of the tree seedlings grown at elevated CO2 increased by about 10%-40% compared to those grown at ambient CO2. And the water using efficiency of seedlings also followed the same tendency. However, the responses of seedlings in transpiration and chlorophyll content to elevated CO2 varied with tree species. The broad-leaf tree species were more sensitive to the elevated CO2 than conifer tree species. All seedlings showed a photosynthetic acclimation to long-term elevated CO2.

Analysis of Security of High-yield Cultivation of Spring Peanut in the Southwest of Henan Province Based on Meteorological Factors

The changes of meteorological factors such as average temperature, pre- cipitation and sunshine duration in the whole growth period and at various growth stages of spring peanut in Dengzhou City during 2001-2016 were analyzed, and their effects on the growth and development of spring peanut were discussed. The results show that from 2001 to 2016, average temperature tended to increase in the growth period of spring peanut in Dengzhou City, but the increasing trend was slow; precipitation showed a decreasing trend on the whole （it increased at the sowing and flowering stage）; sunshine duration reduced year by year （the decreasing trend was different at various growth stages）, but the decrease of sunshine duration was more obvious than that of precipitation. Based on the analysis of meteorological conditions and the growth rules of spring peanut, it is needed to scientifically choose varieties of spring peanut, plan suitable sowing patterns, apply and manage- fertilizer and water to reasonably realize sustainable high-yield of spring peanut based on its safe stable yield.

Relationship of serum vitamin D level on geriatric syndromes and physical performance impairment in elderly hypertensive patients

Objective To investigate the relationship among serum vitamin D levels, physical performance impairment, and geriatric syndromes in elders with hypertension. Methods According to the concentration of vitamin D levels, a total of 143 elderly patients with hypertension were classified into vitamin D deficient group （vitamin D 〈 20 ng/mL, n = 94） and vitamin D appropriate group （vitamin D 〉 20 ng/rnL, n = 49）. Geriatric syndromes and physical performance were assessed by using comprehensive geriatric assessment （CGA）. Correlation among vitamin D levels, geriatric syndromes and physical performance was analyzed. Results No statistical differences were found in various aspects of geriatric syndromes between the two groups （P 〉 0.05）. While correlation analysis indicated that vitamin D levels had a positive association with ADL score （r = 0.235, P 〈 0.01） and a negative association with Morse fall scale score （r = 0.238, P 〈 0.01）. Patients with deficient vitamin D level had longer time both in the Five Time Sit to Stand Test （5tSTS）, （15.765 ± 5.593） and the four-meter walk test [7.440 （5.620, 9.200）], a weaker hand-grip in the grip strength test （28.049 ± 9.522）, and a lower Tinetti performance-oriented mobility assessment （Tinetti POMA） [26 （22, 27）] and Balance subscale of the Tinetti performance-oriented mobility assessment （B-POMA） score [ 14 （12, 16）], compared with appropriate vitamin D level [（13.275 ± 3.692）; 5.810 （4.728, 7.325）]; （31.989 ± 10.217）; [26.5 （25, 28）]; [15 （14, 16）, respectively, all P 〈 0.05]. Furthermore, results of logistic regression indicated that vitamin D was significantly associated with 5tSTS （OR = 1.2, 95% CI = 1.050-1.331, P 〈 0.01）, Tinetti POMA （OR = 3.7, 95% CI：1.284-10.830, P 〈 0.05） and B-POMA （OR = 0.8, 95% CI：0.643-0.973, P 〈 0.05）. Conclusions In elderly hypertensive patients, serum vitamin D deficient level is associated with physical performance impairment. However, no statistical significance was found between vitamin D and geriatric syndromes. Further study is required to investigate possible mechanisms for the association between vitamin D and physical performance.

Detection of 36 antibiotics in coastal waters using high performance liquid chromatography-tandem mass spectrometry

Among pharmaceuticals and personal care products released into the aquatic environment, antibiotics are of particular concern, because of their ubiquity and health effects. Although scientists have recently paid more attention to the threat of antibiotics to coastal ecosystems, researchers have often focused on relatively few antibiotics, because of the absence of suitable analytical methods. We have therefore developed a method for the rapid detection of 36 antibiotic residues in coastal waters, including tetracyclines （TCs）, sulfanilamides （SAs）, and quinolones （QLs）. The method consists of solid-phase extraction （SPE） and liquid chromatography-tandem mass spectrometry （LC-MS/MS） analysis, using electrospray ionization （ESI） in positive mode. The SPE was performed with Oasis HLB and Oasis MCX cartridges. Chromatographic separation on a Cr8 column was achieved using a binary eluent containing methanol and water with 0.1% formic acid. Typical recoveries of the analytes ranged from 67.4% to 109.3% at a fortification level of 100 ng/L. The precision of the method, calculated as relative standard deviation （RSD）, was below 14.6% for all the compounds. The limits of detection （LODs） varied from 0.45 pg to 7.97 pg. The method was applied to detemaine the target analytes in coastal waters of the Yellow Sea in Liaoning, China. Among the tested antibiotics, 31 were found in coastal ＇waters, with their concentrations between the LOD and 212.5 ng/L. These data indicate that this method is valid for analysis of antibiotics in coastal waters. The study first reports such a large number of antibiotics along the Yellow Sea coast of Liaoning, and should facilitate future comprehensive evaluation of antibiotics in coastal ecosystems

Biodegradation of microcystin-RR and -LR by an indigenous bacterial strain MC-LTH11 isolated from Lake Taihu

The indigenous bacterial strain MC-LTH11 with the capability of degrading microcystin-RR MC-RR and microcystin-LR MC-LR was successfully isolated from Lake Taihu.The bacterium was identified as Stenotrophomonas sp. which possessed a mlrA gene. The MC-LTH11 thoroughly degraded MC-RR and MC-LR with the initial concentration of 37.13 mg/L and 18.49 mg /L respectively in the medium containing crude microcystins extract within 6 d.The degradation rates were affected by temperature pH initial MCs concentration and the kinds of media. Additionally the bacterial strain MC-LTH11 also degraded thoroughly microcystins in the water body of Lake Taihu within 1 d.These results suggest that the Stenotrophomonas sp.MC-LTH11 has the capacity to bioremediate water bodies contaminated by microcystins and may contribute to the degradation of microcystins after the outbreak of harmful cyanobacterial blooms in Lake Taihu.

Spatial-temporal Evolution Pattern of Agricultural Productivity in Northwestern Sichuan Plateau

Currently, the agricultural growth in developed countries mainly relies on the improvement of productivity, which is also the target for China. Accordingly, the purpose of this study was to describe the spatial-temporal evolution pattern of agricultural productivity, to reveal changes in total factor productivity in 2ooo-2olo, and analyze the impact of these changes in northwestern Sichuan plateau, China. Using data envelopment analysis （DEA） and the Malmquist Index, an in-depth study was conducted on agricultural productivity and changes in total factor productivity of 31 counties in northwestern Sichuan plateau. Results indicated that： （1） geographically, counties with optimal efficiency were mainly located in the north of northwestern Sichuan plateau and those with the lowest efficiency, in the south; （2） relative to pure technical efficiency, scale efficiency was the dominant factor in determining agricultural productivity; （3） the redundancy rate of input factors in zolo was slightly lower than that in zooo, thereby indicating an improved utilization of input factors to a certain extent and a great potential for further improving such utilization; （4） during the 2ooo-2olo period, the agricultural total factor productivity had an average annual growth rate of 8.3%, but the growth rates in various regions differed widely; （5） technical progress was the dominant factor promoting the improvement of total factor oroductivitv inagriculture. The disparities in spatial distribution may be due to the differences of natural conditions, former level agricultural productivity between counties. The findings are valuable for the government to make sustainable development policies for agriculture and improving agricultural development in northwestern Sichuan plateau.

Agrobacterium-mediated transformation of herbicide resistance in creeping bentgrass and colonial bentgrass

Embryogenic calli were induced from the seeds of creeping bentgrass ( Agrostis palustris Huds.) cv. Regent and colonial bentgrass ( Agrostis Tenuis Sibth. Fl. Oxen.) cv. Tiger. The embryogenic calli were precultured on fresh medium for 4-7 days and then co cultivated with Agrobacterium tumefaciens , LBA4404, which contains plasmid vector pSBGM harboring bar coding region, synthetic green fluorescent protein (sGFP) coding region and matrix attachment region (MAR). After 3 days of co cultivation, the calli were washed thoroughly and transferred to MS medium containing 2 mg/L of 2, 4 D, 12-15 mg/L phosphinothricin (PPT) and 250 mg/L of cefotaxime. After 2-3 months of selection, the actively growing calli of 'Regent' and 'Tiger' were transferred to MS medium with 12-15 mg/L PPT and 250 mg/L cefotaxime for regeneration. The putative transformants were maintained on MS medium with 3 mg/L PPT for long period but control died within 1 month. After establishing in greenhouse, the transformants also showed strong resistance to 0.4% of herbicide Basta but control plants died within 2 weeks. Under confocal microscope, both young leaves and roots showed significant GFP expression. PCR analysis revealed the presence of a DNA fragment of GFP gene at the expected size (380 bp) in the transformants and its absence in a randomly selected control plant.

Diagnosis of Hypophosphatasia

Objective： HPP （Hypophosphatasia） is a congenital disease characterized by a deficiency of tissue-specific ALP （alkaline phosphatase）, which causes the generation of abnormal bone and tooth tissue. The clinical manifestations are variable, from neonatal forms with high mortality to milder adult forms with fractures and osteomalacia. The present study reports a clinical case of HPP, with emphasis on the physical characteristics and laboratory tests. The patient was attended at the medical genetics outpatient clinic of the Association of Parents and Friends of the Exceptional of Manaus （Associa？？o de Pais e Amigos dos Excepcionais-APAE/Manaus）. Methodology： For the case report, anamnesis, peripheral blood G-band karyotyping, Vitamin and ALP dosage and resonance examination were performed. Results： A.M.M.S, 9 years, non-consanguineous parents, no bone disease in the family, with one sister from the mother. Birth weight AGA without intercurrence. The patient presented a slight delay in psycho-motor development and was diagnosed with craniostenosis through CNS imaging examination. The physical examination, in addition to a short stature, showed dolichocephaly, frontal bossing, curved tibias and great dental compromise with many early cavities and loss of teeth. Laboratory evaluation： ALP dosage： 63/L （N = 69-325）; Vitamin B6 dosage： 27.4 （5.2-34.1）. The mother and the younger sister also had an ALP result below that expected for the age. Conclusions： The clinical phenotype and laboratory diagnosis of the patient were compatible with ICD Q78 HPP.

Sap Flow of Abies georgei var. smithii and Its Relationship with the Environment Factors in the Tibetan Subalpine Region, China

Abies georgei var. smithii is a dominant species playing an important role in protecting biodiversity and sustaining the forestry ecosystems in Southeastern Tibetan Plateau. Stem sap flows of five different diameters at the breast height(DBH) A. georgei var. smithii samples were monitored continuously with the thermal dissipation probe for the entire growing period in order to understand the water transportation mechanism and the effects of environmental factors on its transpiration and growth. Relative environment factors, temperature and humidity of air, photosynthetically active radiation, rainfall, and wind speed, soil moisture, etc. were measured by the automatic weather stations. Diurnal and seasonal variations in sap flow rate with the different stem diameters and their correlations with meteorological factors were analyzed. The diurnal change in sap flow velocity showed a single-peak curve at the daily time scale, whereas a lower sap flow velocity can be observed in the largest DBH sample tree at night. The maximum average velocity was observed in August, whereas the minimum velocity was observed in January, and a large amount of water evaporated in summer owing to the higher sap flow velocity. In addition, sap flow velocity was closely related to changes in the micrometeorological factors, with average sap flow velocity showing significant linear correlations with air temperature, photosynthetically active radiation, rainfall, and vapor pressure deficit of air and soil moisture. Therefore, some measures, improving the light and temperature conditions, should be taken for protecting A. georgei var. smithii population in the Tibetan Plateau.

Current Situation of Cultural Conflict Between Teachers and Students in English Classes Provided by Colleges and Universities and Effective Solutions

At present, quality education is proceeding in full swing in China. English education provided by colleges and universities of China has gone through a series of reform and gained relatively rapid development to a certain degree. English has become an international language, and most companies have listed English proficiency as an important requirement for hiring employees, which indicates that English teaching in classrooms of colleges and universities is of great importance. However, cultural conflict between teachers and students in English classes provided by colleges and universities has been gradually drawing people＇s attention and has an impact on quality of English teaching provided by colleges and universities. In this paper, the current situation of cultural conflict is introduced and several solutions for solving such cultural conflicts are put forward, which may be viewed as a reflection.

Construction and Implementation of Practice Teaching System of English Major in Local Normal Universities

English practice teaching has outstanding superiority over the extension and supplementation of English classroom teaching, and practice teaching is an indispensably important part of college English teaching. To students, cultivation of the ability of practice is vital, affecting students＇ employment and future. To universities, teaching quality has a direct impact on the development prospect of schools and decides the future of schools. Moreover, local normal universities also undertake a more important task to cultivate local high-quality talents teaching primary and junior high schools. These teaching talents undertake the great task of English teaching over primary and junior high school students, and thus the construction and implementation of practice teaching system of English major in normal universities are urgent.

Uptake and Recovery of Soil Nitrogen by Bryophytes and Vascular Plants in an Alpine Meadow

Due to their particular physiology and life history traits, bryophytes are critical in regulating biogeochemical cycles and functions in alpine ecosystem. Hence, it is crucial to investigate their nutrient utilization strategies in comparison with vascular plants and understand their responses to the variation of growing season caused by climate change. Firstly, this study testified whether or not bryophytes can absorb nitrogen(N) directly from soil through spiking three chemical forms of 15N stable isotope tracer. Secondly, with stronger ability of carbohydrates assimilation and photosynthesis, it is supposed that N utilization efficiency of vascular plants is significantly higher than that of bryophytes. However, the recovery of soil N by bryophytes can still compete with vascular plants due to their greater phytomass. Thirdly, resource acquisition may be varied from the change of growing season, during which N pulse can be manipulated with 15N tracer addition at different time. Both of bryophytes and vascular plants contain more N in a longer growing season, and prefer inorganic over organic N. Bryophytes assimilate more NH4+ than NO3– and amino acid, which can be indicated from the greater shoot excess 15N of bryophytes. However, vascular plants prefer to absorb NO3– for their developed root systems and vascular tissue. Concerning the uptake of three forms N by bryophytes, there is significant difference between two manipulated lengths of growing season. Furthermore, the capacity of bryophytes to tolerate N-pollution may be lower than currently appreciated, which indicates the effect of climate change on asynchronous variation of soil N pools with plant requirements.

Public Diplomacy Literacy Education of Youth in the Era of New Media Sphere

In recent years, public diplomacy experienced a steady and prosperous development in China. The objective of this paper is to map out the role of public diplomacy in China playing in the process of China＇s national image construction overseas and the necessity of public diplomacy literacy education of youth especially those college students in higher education institutions. To be aware of these, it is significant and valuable in terms of the public diplomacy literacy education in college teaching in the era of new media sphere.