大豆含35～40％蛋白质(干基)。大豆蛋白的主要成分是由大豆球蛋白(llS)和β一伴大豆球蛋白(7S)组成(Derbyshire et al,1976)。一、高硫蛋白的提纯工艺将大豆研磨至40目,用冻丙酮(4℃)将大豆粉脱脂,置于铝制薄片中摊开,在通风槽中干燥。...大豆含35～40％蛋白质(干基)。大豆蛋白的主要成分是由大豆球蛋白(llS)和β一伴大豆球蛋白(7S)组成(Derbyshire et al,1976)。一、高硫蛋白的提纯工艺将大豆研磨至40目,用冻丙酮(4℃)将大豆粉脱脂,置于铝制薄片中摊开,在通风槽中干燥。干大豆粉置于密封容器内,在—20℃下贮存备用。高硫球蛋白提纯工艺见图:展开更多
Drosophila RYBP （dRYBP; Ringl and Y_Y1 Binding Protein） is a Polycomb and trithorax interacting protein, whose homologous RYBP/DEDAF mammalian counterparts exhibit tumor cell-specific killing activity. Here we show ...Drosophila RYBP （dRYBP; Ringl and Y_Y1 Binding Protein） is a Polycomb and trithorax interacting protein, whose homologous RYBP/DEDAF mammalian counterparts exhibit tumor cell-specific killing activity. Here we show that although endogenous dRYBP is not involved in developmental apoptosis, high levels of exogenous dRYBP induce apoptosis in all the imaginal discs of the fly, indicating that dRYBP apoptotic activity is not specific to tumor cells. We also show that dRYBP-induced apoptosis is inhibited by high levels of either p35 or DIAP1 （Drosophila Inhibitor of Apoptosis Protein 1）, and requires the function of the pro-apoptotic REAPER, HID and GRIM proteins, the apical caspase DREDD, the adaptor dFADD protein as well as TRITHORAX （TRX）, an epigenetic transcriptional regulator. Furthermore, we demonstrate that overexpression of TRX also induces apoptosis in the imaginal discs. Finally, we show that the expression of reaper-lacZ is upregulated both upon dRYBP-induced apoptosis and upon TRX- induced apoptosis in imaginal discs and that the reaper gene is a direct target of dRYBP in Drosophila embryos. Our results indicate that dRYBP triggers in a receptor-mediated apoptotic pathway that also includes TRX-dependent epigenetic regulation of gene expression.展开更多
文摘大豆含35～40％蛋白质(干基)。大豆蛋白的主要成分是由大豆球蛋白(llS)和β一伴大豆球蛋白(7S)组成(Derbyshire et al,1976)。一、高硫蛋白的提纯工艺将大豆研磨至40目,用冻丙酮(4℃)将大豆粉脱脂,置于铝制薄片中摊开,在通风槽中干燥。干大豆粉置于密封容器内,在—20℃下贮存备用。高硫球蛋白提纯工艺见图:
文摘Drosophila RYBP （dRYBP; Ringl and Y_Y1 Binding Protein） is a Polycomb and trithorax interacting protein, whose homologous RYBP/DEDAF mammalian counterparts exhibit tumor cell-specific killing activity. Here we show that although endogenous dRYBP is not involved in developmental apoptosis, high levels of exogenous dRYBP induce apoptosis in all the imaginal discs of the fly, indicating that dRYBP apoptotic activity is not specific to tumor cells. We also show that dRYBP-induced apoptosis is inhibited by high levels of either p35 or DIAP1 （Drosophila Inhibitor of Apoptosis Protein 1）, and requires the function of the pro-apoptotic REAPER, HID and GRIM proteins, the apical caspase DREDD, the adaptor dFADD protein as well as TRITHORAX （TRX）, an epigenetic transcriptional regulator. Furthermore, we demonstrate that overexpression of TRX also induces apoptosis in the imaginal discs. Finally, we show that the expression of reaper-lacZ is upregulated both upon dRYBP-induced apoptosis and upon TRX- induced apoptosis in imaginal discs and that the reaper gene is a direct target of dRYBP in Drosophila embryos. Our results indicate that dRYBP triggers in a receptor-mediated apoptotic pathway that also includes TRX-dependent epigenetic regulation of gene expression.
