This paper provides the information on nesting biology of 20 bird species in alpine shrub (4000-4700 m a.s.l.) or alpine meadow-wasteland habitats (4300-4700 m a.s.l.) in Lhasa region,Tibet.Of these species,three are ...This paper provides the information on nesting biology of 20 bird species in alpine shrub (4000-4700 m a.s.l.) or alpine meadow-wasteland habitats (4300-4700 m a.s.l.) in Lhasa region,Tibet.Of these species,three are endemic to the Tibetan plateau and 17 widespread in altitudes.The data on these taxa are all new to the high-altitude environments,especially the upper limit of their breeding distribution.A few species are firstly reported with respect to nesting information in the world.The study will contribute our knowledge for natural history of birds occurring in the harsh,extreme habitats.展开更多
The genetic parameters of main reproductive traits for the new Yorkshire line with high prolificacy were evaluated by multiple animal model DFREML The re- suits showed that the heritability evaluates of the total numb...The genetic parameters of main reproductive traits for the new Yorkshire line with high prolificacy were evaluated by multiple animal model DFREML The re- suits showed that the heritability evaluates of the total number of born （TNB）, alive number of born （ANB）, litter weigh at birth （LWB） and litter weight at age of 21 days were 0.10, 0.15, 0.09 and 0.14, respectively. The genetic correlations between these traits, for example, the correlations between TNB/ANB, NB/LWB, TNB/LW21, ANB/LWB, ANB/LW21 and LWB/LW21 were 0.87, 0.74, 0.43, 0.69, 0.59 and 0.51, respectively.展开更多
The combined selection index used in the breeding of new Yorkshire dam line with high prolificacy according to breeding objects was formulated as /：2.272E- BVNB-0.056EBVDAYS. After 5 generations breeding, the two mai...The combined selection index used in the breeding of new Yorkshire dam line with high prolificacy according to breeding objects was formulated as /：2.272E- BVNB-0.056EBVDAYS. After 5 generations breeding, the two main selected traits such as total number of born and age at 100 kg weight was 12.17 piglets/litter and 165.18 d, respectively. The genetic improvements per generates was 0.156 and -2.198, respec- tively. The breeding objects of the new Yorkshire dam line with high prolificacy were basically reached. It indicated that the methods and index could be used in pig breeding.展开更多
The effects of temperature, initial pH, salinity of culture medium, and carbon and nitrogen sources on growth and doco- sahexaenoic acid (C22 : 6 n-3, DHA) production from Schizochytrium limacinum OUC88 were investiga...The effects of temperature, initial pH, salinity of culture medium, and carbon and nitrogen sources on growth and doco- sahexaenoic acid (C22 : 6 n-3, DHA) production from Schizochytrium limacinum OUC88 were investigated in the present study. The results revealed that the optimal temperature, initial pH and salinity level of the medium for DHA production were 23℃, 7.0 and 18, respectively. Glucose was proved the best carbon source for the growth and DHA production from S. limacinum. Among the nitrogen sources tested, soybean cake hydrolysate, a cheap by-product, was found to be effective for the accumulation of DHA in S. lima- cinum cells. In addition, increasing the concentration of carbon sources in the medium caused a significant increase in cell biomass; however, accumulation of DHA in cells was mainly stimulated by the ratio of C/N in the medium. Under the optimal culture condi- tions, the maximum DHA yield achieved in flasks was 4.08 g L-1 after 5 d of cultivation.展开更多
The phenotypic traits of high prolificacy, low fat on the rib eye muscle and high tolerance to internal parasites make the Barbados Blackbelly （BBB） sheep a valuable economic resource for small subsistence farmers o...The phenotypic traits of high prolificacy, low fat on the rib eye muscle and high tolerance to internal parasites make the Barbados Blackbelly （BBB） sheep a valuable economic resource for small subsistence farmers on some Caribbean islands. However, the determination of what is a purebred BBB sheep has long been a contentious issue because of the subjectivity. It is therefore necessary to have a more reliable means of identifying what is a purebred BBB sheep for purposes of conservation and breed improvement. Three geographically isolated populations of BBB sheep were genetically analysed at 19 microsatellite loci. Eighteen of the microsatellites were found to be useful for genetic analysis of the BBB sheep, based on polymorphic information content （PIC） values （0.5 〈 PIC 〉 0.25）. One microsatellite was not useful for genetic analysis based on PIC （0.111）, however it is considered to be a locus of interest because it is fully inbred （f = 1） and homozygous in all of the populations analysed. The analysis also showed that the purebred population of BBB sheep can be distinguished from other populations by formation of distinct clusters when subjected to analysis of molecular variance （AMOVA） and the construction of a rooted consensus tree using the Neighbour-Joining method and pair-wise distances based on marker estimated kinships.展开更多
[Objective] The purpose of the study is to breed Micromonospora car- bonacea highly producing antibiotics and then to improve the antibiotic production. [Method] Sodium Nitrite mutagenesis, combined with rifampicin re...[Objective] The purpose of the study is to breed Micromonospora car- bonacea highly producing antibiotics and then to improve the antibiotic production. [Method] Sodium Nitrite mutagenesis, combined with rifampicin resistance screening, was used in mutation breeding of M. carbonacea highly producing antibiotics from the strain of M. carbonacea JXNU-I. [Result] The overproducing strain JXNU-1-16- Y65 was screened with the production of antibiotics 266.05% more than that of the original strain. [Conclusion] The effectiveness of sodium nitrite mutation in breeding microorganisms highly producing antibiotic was proved, and the study may lay the foundation on further development and application of the antibiotic from M. car- bonacea JXNU-1.展开更多
Ultrasound has been widely used in clinics. Cellular responses to low-intensity ultrasound are parameter-dependent. Proper parameter setting is vital to its exact use. To get guidelines for parameter setting, lowinten...Ultrasound has been widely used in clinics. Cellular responses to low-intensity ultrasound are parameter-dependent. Proper parameter setting is vital to its exact use. To get guidelines for parameter setting, lowintensity ultrasound stimulation on the proliferation and reproductivity of Hep G2 and 3T3 cells in vitro was examined with a 1.06 MHz-generator by changing the parameters(including intensity, pulse repetition frequency and duty cycle)in a wide range. Cell viability and reproductivity at different time after sonication were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)and colony formation assay to indicate timerelated proliferation. The results illustrate that ultrasound irradiation at 0.4—0.8 W/cm^2 and high pulse repetition frequency(100 Hz)can facilitate cell proliferation, while above 0.8 W/cm^2 would resist it. The extent of resistance closely correlated with duty cycle and pulse repetition frequency. Resistance effect at low pulse repetition frequency(1 Hz)is greater than that at high pulse repetition frequency(100 Hz)and not time-related. The influence of high pulse repetition frequency is time-accumulated, indicating cellular process involved. These findings would provide valuable guidelines for the application of low-intensity ultrasound in stem cell transformation and tissue engineering.展开更多
基金the National Natural Sciences Foundation of China (Grant Nos. 39800016,30170156,30270216,30370241,30425036,30670337,and 30830019)
文摘This paper provides the information on nesting biology of 20 bird species in alpine shrub (4000-4700 m a.s.l.) or alpine meadow-wasteland habitats (4300-4700 m a.s.l.) in Lhasa region,Tibet.Of these species,three are endemic to the Tibetan plateau and 17 widespread in altitudes.The data on these taxa are all new to the high-altitude environments,especially the upper limit of their breeding distribution.A few species are firstly reported with respect to nesting information in the world.The study will contribute our knowledge for natural history of birds occurring in the harsh,extreme habitats.
基金Supported by National Science and Technology Support Plan during the Eleventh Five-year Plan(2006BAD01A08-02)Hubei Agricultural Innovation Program(2007-620-004-003)Special Fund for Modern Pig Production Technology Construction(NYCYTX-009)~~
文摘The genetic parameters of main reproductive traits for the new Yorkshire line with high prolificacy were evaluated by multiple animal model DFREML The re- suits showed that the heritability evaluates of the total number of born （TNB）, alive number of born （ANB）, litter weigh at birth （LWB） and litter weight at age of 21 days were 0.10, 0.15, 0.09 and 0.14, respectively. The genetic correlations between these traits, for example, the correlations between TNB/ANB, NB/LWB, TNB/LW21, ANB/LWB, ANB/LW21 and LWB/LW21 were 0.87, 0.74, 0.43, 0.69, 0.59 and 0.51, respectively.
基金Supported by National Science and Technology Support Plan during the Eleventh Five-year Plan(2006BAD01A08-02)Hubei Agricultural Innovation Program(2007-620-004-003)Special Fund for Modern Pig Production Technology Construction(NYCYTX-009)~~
文摘The combined selection index used in the breeding of new Yorkshire dam line with high prolificacy according to breeding objects was formulated as /：2.272E- BVNB-0.056EBVDAYS. After 5 generations breeding, the two main selected traits such as total number of born and age at 100 kg weight was 12.17 piglets/litter and 165.18 d, respectively. The genetic improvements per generates was 0.156 and -2.198, respec- tively. The breeding objects of the new Yorkshire dam line with high prolificacy were basically reached. It indicated that the methods and index could be used in pig breeding.
文摘The effects of temperature, initial pH, salinity of culture medium, and carbon and nitrogen sources on growth and doco- sahexaenoic acid (C22 : 6 n-3, DHA) production from Schizochytrium limacinum OUC88 were investigated in the present study. The results revealed that the optimal temperature, initial pH and salinity level of the medium for DHA production were 23℃, 7.0 and 18, respectively. Glucose was proved the best carbon source for the growth and DHA production from S. limacinum. Among the nitrogen sources tested, soybean cake hydrolysate, a cheap by-product, was found to be effective for the accumulation of DHA in S. lima- cinum cells. In addition, increasing the concentration of carbon sources in the medium caused a significant increase in cell biomass; however, accumulation of DHA in cells was mainly stimulated by the ratio of C/N in the medium. Under the optimal culture condi- tions, the maximum DHA yield achieved in flasks was 4.08 g L-1 after 5 d of cultivation.
文摘The phenotypic traits of high prolificacy, low fat on the rib eye muscle and high tolerance to internal parasites make the Barbados Blackbelly （BBB） sheep a valuable economic resource for small subsistence farmers on some Caribbean islands. However, the determination of what is a purebred BBB sheep has long been a contentious issue because of the subjectivity. It is therefore necessary to have a more reliable means of identifying what is a purebred BBB sheep for purposes of conservation and breed improvement. Three geographically isolated populations of BBB sheep were genetically analysed at 19 microsatellite loci. Eighteen of the microsatellites were found to be useful for genetic analysis of the BBB sheep, based on polymorphic information content （PIC） values （0.5 〈 PIC 〉 0.25）. One microsatellite was not useful for genetic analysis based on PIC （0.111）, however it is considered to be a locus of interest because it is fully inbred （f = 1） and homozygous in all of the populations analysed. The analysis also showed that the purebred population of BBB sheep can be distinguished from other populations by formation of distinct clusters when subjected to analysis of molecular variance （AMOVA） and the construction of a rooted consensus tree using the Neighbour-Joining method and pair-wise distances based on marker estimated kinships.
基金Supported by Science and Technology Planning Project of Jiangxi Province of China(20112BBF60026)~~
文摘[Objective] The purpose of the study is to breed Micromonospora car- bonacea highly producing antibiotics and then to improve the antibiotic production. [Method] Sodium Nitrite mutagenesis, combined with rifampicin resistance screening, was used in mutation breeding of M. carbonacea highly producing antibiotics from the strain of M. carbonacea JXNU-I. [Result] The overproducing strain JXNU-1-16- Y65 was screened with the production of antibiotics 266.05% more than that of the original strain. [Conclusion] The effectiveness of sodium nitrite mutation in breeding microorganisms highly producing antibiotic was proved, and the study may lay the foundation on further development and application of the antibiotic from M. car- bonacea JXNU-1.
基金Supported by the Natural Science Foundation of Tianjin(No.12JCYBJC18300)
文摘Ultrasound has been widely used in clinics. Cellular responses to low-intensity ultrasound are parameter-dependent. Proper parameter setting is vital to its exact use. To get guidelines for parameter setting, lowintensity ultrasound stimulation on the proliferation and reproductivity of Hep G2 and 3T3 cells in vitro was examined with a 1.06 MHz-generator by changing the parameters(including intensity, pulse repetition frequency and duty cycle)in a wide range. Cell viability and reproductivity at different time after sonication were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)and colony formation assay to indicate timerelated proliferation. The results illustrate that ultrasound irradiation at 0.4—0.8 W/cm^2 and high pulse repetition frequency(100 Hz)can facilitate cell proliferation, while above 0.8 W/cm^2 would resist it. The extent of resistance closely correlated with duty cycle and pulse repetition frequency. Resistance effect at low pulse repetition frequency(1 Hz)is greater than that at high pulse repetition frequency(100 Hz)and not time-related. The influence of high pulse repetition frequency is time-accumulated, indicating cellular process involved. These findings would provide valuable guidelines for the application of low-intensity ultrasound in stem cell transformation and tissue engineering.
