Objective.To evaluate the effects of high-fructose diet on expression of orex in and its receptors,orexin1receptor and orexin2receptor in rat hypothalamus tissue,and to analysis the interaction of related fa ctors inv...Objective.To evaluate the effects of high-fructose diet on expression of orex in and its receptors,orexin1receptor and orexin2receptor in rat hypothalamus tissue,and to analysis the interaction of related fa ctors involved in regulating orexin and its receptors.Methods.Insulin resistan ce rat model induced by high fructose confirmed by the gold standard eug-lycaem ic clamping was employed and mRNA expression of orexin and its receptors OX 1 R and OX 2 R in hypothalamus,mRNA expression of leptin in adipose tissue were measured by reverse transcription poly-merase chain reaction.Serum insulin and triglyc eride levels were measured by chemiluminescence im-munoassay and biochemical en zyme techniques.Results.Expression of orexin mRNA decreased about 40%in hi gh fructose diet rats compared to control group,whereas expressi on of orexin1receptor and orexin2receptor mRNA increased up to4.4and5.1f old.Leptin mRNA expression in adipose tissue increased about 3 0%in comparison with control group.Blood glucose,serum insuli n and triglyceride have shown signi ?ficant higher levels than those in contro l group.Glucose infusion rate was much low-er in comparison with control group.Conclusi ons.High?fructose diet induces insulin resistance in rats with impact on orexi n and leptin regulations.Blood glucose,serum insulin,lipid metabolism and lep tin play an interactive role on orexin and its receptors regulation in rats.展开更多
AIM:To evaluate the effects of soy supplementation on insulin resistance,fatty liver and alanine aminotransferase(ALT) levels in non-diabetic patients with chronic hepatitis C(CHC).METHODS:In a prospective,randomized ...AIM:To evaluate the effects of soy supplementation on insulin resistance,fatty liver and alanine aminotransferase(ALT) levels in non-diabetic patients with chronic hepatitis C(CHC).METHODS:In a prospective,randomized and singleblinded clinical trial,we compared patients with CHC who had casein as a supplement(n = 80)(control group),with patients who consumed a soy supplement diet(n = 80) [intervention group(IG)].Both groups received 32 g/d of protein for 12 wk.RESULTS:Patients' baseline features showed that 48.1% were overweight,43.7% had abdominal fat accumulation,34.7% had hepatic steatosis and 36.3% had an homeostasis model assessment index of insulin resistance(HOMA-IR) ≥ 3.0.Descriptive analysis showed that protein supplementation diet reduced hepatic steatosis in both groups;however,significant reductions in ALT levels occurred in the soy group.Multiple regression modeling indicated that in the presence of severe fibrosis(F3/F4),g glutamyl transferase elevation and high density lipoprotein(HDL) reduction,the intervention group had 75% less chance of developing hepatic steatosis(OR= 0.25;95% CI:0.06-0.82) and 55% less chance of presenting with an ALT level ≥ 1.5 × the upper limit of normal(ULN)(OR = 0.45,95% CI:0.22-0.89).Soy treatment did not have any effect on insulin resistance(OR = 1.92;95% CI:0.80-4.83),which might be attributed to the fact that the HOMAIR values at baseline in most of our patients were in the normal range.Advanced hepatic fibrosis,an ALT level > 1.5 × ULN and visceral fat were predictors of an HOMA-IR ≥ 3.The IG group had a reduced risk of an ALT level > 1.5 × ULN.An HOMA-IR ≥ 3.0 and HDL < 35 mg/dL were also risk factors for increased ALT.CONCLUSION:Soy supplementation decreased ALT levels and thus may improve liver inflammation in hepatitis C virus(HCV) patients;it also reduced hepatic steatosis in a subgroup of patients but did not change insulin resistance.It should be considered in the nutritional care of HCV patients.展开更多
Objective To assess the risk factors for prehypertension in Xinjiang Uygur population. Methods A cross-section study was conducted in a Xinjiang Uygur population（438 males and 716 females,aged 30 to 70 years）.The fa...Objective To assess the risk factors for prehypertension in Xinjiang Uygur population. Methods A cross-section study was conducted in a Xinjiang Uygur population（438 males and 716 females,aged 30 to 70 years）.The fasting lipid profiles,serum glucose,insulin,and uric acid were determined.Homeostasis model assessment of insulin resistance（HOMA-IR）index was used to assess insulin resistance（IR）.Binary logistic regression analysis was performed to determine risk factors for prehypertension.Blood pressure levels ofnormotensives and prehypertensives in different body mass index（BMI）categories were compared. Results Binary logistic regression analysis performed after adjustment for gender,lipids profiles,waist-to-hip ratio,uric acid,HOMA-IR,and lifestyle（alcohol drinking and smoking）showed a significantly increasing prevalence of prehypertension with BMI.The odds ratios for prehypertension against the lowest BMI group（separated by 24 and 28）were 1.934 and 2.490（95% confidence interval：1.435-2.606 and 1.825-3.399,respectively）.Age was independently correlated to the increasing prevalence of prehypertension.HOMA-IR was not associated with prehypertensive.The mean diastolic blood pressure（DBP）was significantly increased with BMI categories in either normotensives or prehypertensives（P〈0.001）while the mean systolic blood pressure（SBP）was significantly increased with BMI only in normotensives（P〈0.001）. Conclusions In Xinjiang Uygurs,BMI and age was the risk factors for prehypertension.DBP is significantly increased with BMI.IR is not associated with prehypertension.These findings emphasize the importance of management of obesity for the control of blood pressure and other cardiovascular complications.展开更多
OBJECTIVE:To study serum visfatin levels in women with polycystic ovary syndrome(PCOS)grouped by Traditional Chinese Medicine(TCM)patterns.To study the correlations of serum visfatin levels with homeostatic model asse...OBJECTIVE:To study serum visfatin levels in women with polycystic ovary syndrome(PCOS)grouped by Traditional Chinese Medicine(TCM)patterns.To study the correlations of serum visfatin levels with homeostatic model assessment insulin resistance(HOMA-IR),fasting plasma glucose(FPG),fasting insulin(FINS),body mass index(BMI),testosterone(T),total cholesterol(TC),and triglycerides(TG).METHODS:Two hundred and twelve PCOS patients were placed into the following TCM pattern subgroups:Kidney-Yang deficiency(KYD)group,Spleen-Yang deficiency(SYD)group,stagnant Liver-Qi transforming into heat(SLQTH)group,and Kidney-Yin deficiency(KYIND)group.The correlations between serum visfatin levels and HOMA-IR,FPG,FINS,BMI,T,TC,andTG were analyzed.RESULTS:Of all patients with PCOS,there were 82in the KYD group(38.6%),67 in the SYD group(31.6%),37 in the SLQTH group(17.5%),and 26 in the KYIND group(12.3%).Visfatin levels in all PCOS subgroups were higher than those in the control group(P<0.01 or P<0.05).Among these subgroups,the visfatin levels in the SYD group were significantly higher than those in the other three TCM pattern groups(P<0.05).There were no statistical differences among the remaining three pattern groups.The levels of BMI,FINS,HOMA-IR,T,and TG were significantly higher in all subgroups than those in the control group(P<0.05).There were no significant differences in FPG and TC between all PCOS subgroups and the control group(P>0.05).The SYD group had higher levels of FINS and HOMA-IR compared with the KYD,SLQTH,and KYIND groups(P<0.05).In all subgroups,after controlling for BMI,TG,TC,and age,visfatin was positively correlated with FINS(r=0.197,P=0.015)and HOMA-IR(r=0.173,P=0.033),and was not correlated with T.CONCLUSION:KYD and SYD patterns are most common in PCOS patients.Increased visfatin is a common pathophysiologic manifestation in PCOS patients.The SYD group had the highest levels of visfatin,and visfatin was positively correlated with FINS and HOMA-IR.展开更多
One major function of adipocytes is to store excess energy in the form of triglycerides. Insufficient adipose lipid storage is asso- ciated with many pathological conditions including hyperlipidemia, insulin resistanc...One major function of adipocytes is to store excess energy in the form of triglycerides. Insufficient adipose lipid storage is asso- ciated with many pathological conditions including hyperlipidemia, insulin resistance, and type 2 diabetes. In this study, we observed the overexpression of SUMO-specific protease 2 （Senp2） in adipose tissues during obesity. Adipocyte 5enp2 deficiency resulted in less adipose lipid storage accompanied by an ectopic fat accumulation and insulin resistance under high-fat diet feeding. We further found that SET domain bifurcated 1 （Setdbl） was a SUMOylated protein and that SUMOylation promoted Setdbl occupancy on the promoter locus of Pparg and Cebpa genes to suppress their expressions by H3Kgme3. Senp2 could suppress Setdbl function by de-SUMOylation. In adipocyte 5enp2-deficiency mice, accumulation of the SUMOylated Setdbl suppressed the expression of Pparg and Cebpo genes as welt as lipid metabolism-related target genes, which would decrease the ability of lipid storage in adipocytes. These results revealed the crucial role of Senp2-Setdbl axis in controlling adipose lipid storage.展开更多
OBJECTIVE: To investigate the clinical effect of Qingxue Dan(QXD) on obesity and metabolic biomarker related to obesity.METHODS: A randomized, double blinded, placebo-controlled trial with a paralleled study design wa...OBJECTIVE: To investigate the clinical effect of Qingxue Dan(QXD) on obesity and metabolic biomarker related to obesity.METHODS: A randomized, double blinded, placebo-controlled trial with a paralleled study design was conducted. Twenty-six obese volunteers aged between 30 and 60 with obesity and more than 2 metabolic risk factors were recruited at the department of oriental rehabilitation medicine, Kyunghee university oriental medical hospital, Seoul, Korea. Subjects were randomly assigned to an intervention(QXD) group or a placebo group, and treated with 900 mg/d of QXD or placebo medicine for 8 weeks. Primary endpoint was the change of body mass index(BMI) at 8 week from baseline. Secondary outcomes included the change of body composition, abdominal fat mass measured with Dual energy X-ray absorptiometry(DXA), blood pressure,lipid profiles and the homeostasis model assessment for insulin resistance(HOMA-IR).RESULTS: BMI was decreased in the QXD group sig-nificantly. Total body fat, abdominal fat mass measured with DXA Region of Interest and waist circumference(WC) showed a trend toward decreasing in the QXD group, but fat free mass was decreased in all groups. Triglyceride(TG) was decreased in QXD group significantly, but WC, total cholesterol and high-density lipoprotein cholesterol were increased in both group. BP didn't change during the study period. HOMA-IR is decreased in both groups without group effect.CONCLUSION: 8-weeks of oral administrations of QXD(900 mg/d) reduces BMI, with a tendency of lose of total body fat mass, especially abdominal fat. It also significantly reduced serum TG level.These results suggest QXD could be used to treat obesity and metabolic risk factors. Further study is needed to confirm our pilot findings.展开更多
文摘Objective.To evaluate the effects of high-fructose diet on expression of orex in and its receptors,orexin1receptor and orexin2receptor in rat hypothalamus tissue,and to analysis the interaction of related fa ctors involved in regulating orexin and its receptors.Methods.Insulin resistan ce rat model induced by high fructose confirmed by the gold standard eug-lycaem ic clamping was employed and mRNA expression of orexin and its receptors OX 1 R and OX 2 R in hypothalamus,mRNA expression of leptin in adipose tissue were measured by reverse transcription poly-merase chain reaction.Serum insulin and triglyc eride levels were measured by chemiluminescence im-munoassay and biochemical en zyme techniques.Results.Expression of orexin mRNA decreased about 40%in hi gh fructose diet rats compared to control group,whereas expressi on of orexin1receptor and orexin2receptor mRNA increased up to4.4and5.1f old.Leptin mRNA expression in adipose tissue increased about 3 0%in comparison with control group.Blood glucose,serum insuli n and triglyceride have shown signi ?ficant higher levels than those in contro l group.Glucose infusion rate was much low-er in comparison with control group.Conclusi ons.High?fructose diet induces insulin resistance in rats with impact on orexi n and leptin regulations.Blood glucose,serum insulin,lipid metabolism and lep tin play an interactive role on orexin and its receptors regulation in rats.
基金Supported by Gold Nutrition Indústria e Comercio and Centro colaborador Nordeste II/Ministério da Saúde,Brazil
文摘AIM:To evaluate the effects of soy supplementation on insulin resistance,fatty liver and alanine aminotransferase(ALT) levels in non-diabetic patients with chronic hepatitis C(CHC).METHODS:In a prospective,randomized and singleblinded clinical trial,we compared patients with CHC who had casein as a supplement(n = 80)(control group),with patients who consumed a soy supplement diet(n = 80) [intervention group(IG)].Both groups received 32 g/d of protein for 12 wk.RESULTS:Patients' baseline features showed that 48.1% were overweight,43.7% had abdominal fat accumulation,34.7% had hepatic steatosis and 36.3% had an homeostasis model assessment index of insulin resistance(HOMA-IR) ≥ 3.0.Descriptive analysis showed that protein supplementation diet reduced hepatic steatosis in both groups;however,significant reductions in ALT levels occurred in the soy group.Multiple regression modeling indicated that in the presence of severe fibrosis(F3/F4),g glutamyl transferase elevation and high density lipoprotein(HDL) reduction,the intervention group had 75% less chance of developing hepatic steatosis(OR= 0.25;95% CI:0.06-0.82) and 55% less chance of presenting with an ALT level ≥ 1.5 × the upper limit of normal(ULN)(OR = 0.45,95% CI:0.22-0.89).Soy treatment did not have any effect on insulin resistance(OR = 1.92;95% CI:0.80-4.83),which might be attributed to the fact that the HOMAIR values at baseline in most of our patients were in the normal range.Advanced hepatic fibrosis,an ALT level > 1.5 × ULN and visceral fat were predictors of an HOMA-IR ≥ 3.The IG group had a reduced risk of an ALT level > 1.5 × ULN.An HOMA-IR ≥ 3.0 and HDL < 35 mg/dL were also risk factors for increased ALT.CONCLUSION:Soy supplementation decreased ALT levels and thus may improve liver inflammation in hepatitis C virus(HCV) patients;it also reduced hepatic steatosis in a subgroup of patients but did not change insulin resistance.It should be considered in the nutritional care of HCV patients.
基金This study was supported by the National Natural Science Foundation of China （30260038） and the National Supporting Programs for Critical Illness of China （2002BA711A0B）. We are very grateful for the participants from Hetian region. We would also like to thank all the staff of the Center of Diagnosis, Treatment and Research of Hypertension in Xinjiang for support with the medical examination and demographic data collection.
文摘Objective To assess the risk factors for prehypertension in Xinjiang Uygur population. Methods A cross-section study was conducted in a Xinjiang Uygur population（438 males and 716 females,aged 30 to 70 years）.The fasting lipid profiles,serum glucose,insulin,and uric acid were determined.Homeostasis model assessment of insulin resistance（HOMA-IR）index was used to assess insulin resistance（IR）.Binary logistic regression analysis was performed to determine risk factors for prehypertension.Blood pressure levels ofnormotensives and prehypertensives in different body mass index（BMI）categories were compared. Results Binary logistic regression analysis performed after adjustment for gender,lipids profiles,waist-to-hip ratio,uric acid,HOMA-IR,and lifestyle（alcohol drinking and smoking）showed a significantly increasing prevalence of prehypertension with BMI.The odds ratios for prehypertension against the lowest BMI group（separated by 24 and 28）were 1.934 and 2.490（95% confidence interval：1.435-2.606 and 1.825-3.399,respectively）.Age was independently correlated to the increasing prevalence of prehypertension.HOMA-IR was not associated with prehypertensive.The mean diastolic blood pressure（DBP）was significantly increased with BMI categories in either normotensives or prehypertensives（P〈0.001）while the mean systolic blood pressure（SBP）was significantly increased with BMI only in normotensives（P〈0.001）. Conclusions In Xinjiang Uygurs,BMI and age was the risk factors for prehypertension.DBP is significantly increased with BMI.IR is not associated with prehypertension.These findings emphasize the importance of management of obesity for the control of blood pressure and other cardiovascular complications.
基金Supported by Traditional Chinese Medicine Project of Chongqing Municipal Health Bureau,China
文摘OBJECTIVE:To study serum visfatin levels in women with polycystic ovary syndrome(PCOS)grouped by Traditional Chinese Medicine(TCM)patterns.To study the correlations of serum visfatin levels with homeostatic model assessment insulin resistance(HOMA-IR),fasting plasma glucose(FPG),fasting insulin(FINS),body mass index(BMI),testosterone(T),total cholesterol(TC),and triglycerides(TG).METHODS:Two hundred and twelve PCOS patients were placed into the following TCM pattern subgroups:Kidney-Yang deficiency(KYD)group,Spleen-Yang deficiency(SYD)group,stagnant Liver-Qi transforming into heat(SLQTH)group,and Kidney-Yin deficiency(KYIND)group.The correlations between serum visfatin levels and HOMA-IR,FPG,FINS,BMI,T,TC,andTG were analyzed.RESULTS:Of all patients with PCOS,there were 82in the KYD group(38.6%),67 in the SYD group(31.6%),37 in the SLQTH group(17.5%),and 26 in the KYIND group(12.3%).Visfatin levels in all PCOS subgroups were higher than those in the control group(P<0.01 or P<0.05).Among these subgroups,the visfatin levels in the SYD group were significantly higher than those in the other three TCM pattern groups(P<0.05).There were no statistical differences among the remaining three pattern groups.The levels of BMI,FINS,HOMA-IR,T,and TG were significantly higher in all subgroups than those in the control group(P<0.05).There were no significant differences in FPG and TC between all PCOS subgroups and the control group(P>0.05).The SYD group had higher levels of FINS and HOMA-IR compared with the KYD,SLQTH,and KYIND groups(P<0.05).In all subgroups,after controlling for BMI,TG,TC,and age,visfatin was positively correlated with FINS(r=0.197,P=0.015)and HOMA-IR(r=0.173,P=0.033),and was not correlated with T.CONCLUSION:KYD and SYD patterns are most common in PCOS patients.Increased visfatin is a common pathophysiologic manifestation in PCOS patients.The SYD group had the highest levels of visfatin,and visfatin was positively correlated with FINS and HOMA-IR.
基金This work was supported by grants from the National Natural Science Foundation of China （91019021 and 81430069 to J.C.）, the National Basic Research Program of China （973 Program） （2013CB910902 to J.C.）, Shanghai Committee of Science and Technology （15ZR1424500 to T.W. and 15140904300）, Shanghai Municipal Education Commission （ZZjdyx15003 to T.W. and 2017-01-07-00-01-E00050 to J.C.）, and Shanghai Jiao Tong University School of Medicine （14XJ10001 to T.W.）.
文摘One major function of adipocytes is to store excess energy in the form of triglycerides. Insufficient adipose lipid storage is asso- ciated with many pathological conditions including hyperlipidemia, insulin resistance, and type 2 diabetes. In this study, we observed the overexpression of SUMO-specific protease 2 （Senp2） in adipose tissues during obesity. Adipocyte 5enp2 deficiency resulted in less adipose lipid storage accompanied by an ectopic fat accumulation and insulin resistance under high-fat diet feeding. We further found that SET domain bifurcated 1 （Setdbl） was a SUMOylated protein and that SUMOylation promoted Setdbl occupancy on the promoter locus of Pparg and Cebpa genes to suppress their expressions by H3Kgme3. Senp2 could suppress Setdbl function by de-SUMOylation. In adipocyte 5enp2-deficiency mice, accumulation of the SUMOylated Setdbl suppressed the expression of Pparg and Cebpo genes as welt as lipid metabolism-related target genes, which would decrease the ability of lipid storage in adipocytes. These results revealed the crucial role of Senp2-Setdbl axis in controlling adipose lipid storage.
文摘OBJECTIVE: To investigate the clinical effect of Qingxue Dan(QXD) on obesity and metabolic biomarker related to obesity.METHODS: A randomized, double blinded, placebo-controlled trial with a paralleled study design was conducted. Twenty-six obese volunteers aged between 30 and 60 with obesity and more than 2 metabolic risk factors were recruited at the department of oriental rehabilitation medicine, Kyunghee university oriental medical hospital, Seoul, Korea. Subjects were randomly assigned to an intervention(QXD) group or a placebo group, and treated with 900 mg/d of QXD or placebo medicine for 8 weeks. Primary endpoint was the change of body mass index(BMI) at 8 week from baseline. Secondary outcomes included the change of body composition, abdominal fat mass measured with Dual energy X-ray absorptiometry(DXA), blood pressure,lipid profiles and the homeostasis model assessment for insulin resistance(HOMA-IR).RESULTS: BMI was decreased in the QXD group sig-nificantly. Total body fat, abdominal fat mass measured with DXA Region of Interest and waist circumference(WC) showed a trend toward decreasing in the QXD group, but fat free mass was decreased in all groups. Triglyceride(TG) was decreased in QXD group significantly, but WC, total cholesterol and high-density lipoprotein cholesterol were increased in both group. BP didn't change during the study period. HOMA-IR is decreased in both groups without group effect.CONCLUSION: 8-weeks of oral administrations of QXD(900 mg/d) reduces BMI, with a tendency of lose of total body fat mass, especially abdominal fat. It also significantly reduced serum TG level.These results suggest QXD could be used to treat obesity and metabolic risk factors. Further study is needed to confirm our pilot findings.
