Objective To observe therapeutic effects of acupuncture on the mouse of hyperlipemia and to explore the mechanisms. Methods One hundred and twenty Kunming mice, male, sanitary degree, were randomly divided into normal...Objective To observe therapeutic effects of acupuncture on the mouse of hyperlipemia and to explore the mechanisms. Methods One hundred and twenty Kunming mice, male, sanitary degree, were randomly divided into normal group （n=40）, a model group （n=40）, an acupuncture group （n=20） and a medicine group （n=20）. Except the normal group, the mice were fed with high fat forage to prepare mouse hyperlipemia model. On the 15th day of modeling, serum total cholesterol （TC）, triglyceride （TG）, low density lipoprotein-cholesterol （LDL-C）, high density lipoprotein-cholesterol （HDL-C） levels were detected in 20 normal mice and 20 model mice; electroacupuncture was given at bilateral ＂Fēnglóng＂ （丰隆 ST 40）, ＂Qūchí＂ （曲池 LI 11）, ＂Sānyīnjiāo＂ （三阴交 SP 6） in the acupuncture group, once a day, for 10 consecutive days; in the medicine group, the mice were intragastrically administrated with Simvastatin, once daily , for 10 consecutive days. After the end of treatment, serum TC, TG, LDL-C, HDL-C, malondialdehyde （MDA）, nitric oxide （NO） and endothelin （ET） contents and superoxide dismutase （SOD） activities were detected in the groups. Results On the 15th day of modeling, in the model group serum TG, TC, LDL-C contents were significantly higher （all P〈0.05） and HDL-C content was significantly lower （P〈0.01） than those in the normal group. After treatment for 10 days, in the acupuncture group and the medicine group serum TG, TC, LDL-C, MDA and ET contents were significantly lower and serum HDL-C and NO contents and SOD activities were significantly higher than those in the model group （P〈0.05, P〈0.01）, and the improving action in the acupuncture group was better than that in the medicine group （P〈0.05, P〈0.01）. Conclusion Acupuncture can regulate fat metabolism, resist lipid peroxidation and protect vascular endothelial function in the mouse of hyperlipemia.展开更多
OBJECTIVE： To explore the plasma metabolite pro- files in patients with the syndrome of phlegm and blood stasis in hyperlipidemia and atherosclerosis （As）, and to search for the metabolic biomarkers of the syndrome...OBJECTIVE： To explore the plasma metabolite pro- files in patients with the syndrome of phlegm and blood stasis in hyperlipidemia and atherosclerosis （As）, and to search for the metabolic biomarkers of the syndrome. METHODS： The plasma metabolite profiles of 31 patients with the syndrome of phlegm and blood stasis in hyperlipidemia and As, 6 patients with syn- dromes without phlegm and blood stasis, and 10 healthy subjects were analyzed by gas chromatog- raphy-mass spectrometry （GC-MS）. Partial least squares-discriminant analyses （PLS-DA） were used to carry out the pattern-recognition analyses of the data. The plasma metabolic biomarkers of patients were obtained by variable importance plot value （VlP value） and Student＇s t-test. The structures ofbiomarkers were defined by the National Institute of Standards and Technology （NIST） database. RESULTS： PLS-DA score plots of plasma metabo- Iomes did not show overlap between the phlegm-blood stasis syndrome group and syn- dromes without phlegm and blood stasis group, whereas significant differences in the concentra- tions in the plasma of 5 metabolites were found （P〈 0.05）. They were identified as urine, isoleucine, gluc- uronic acid, palmitic acid and glycerol by searching in NIST database. The concentrations of four metab- olites in the plasma of patients with syndrome of phlegm and blood stasis were higher than those with syndromes without phlegm and blood stasis, whereas the glycerol concentration was lower. CONCLUSION： Compared with patients with syn- dromes without phlegm and blood stasis, five me- tabolites showed abnormal levels in patients with the syndrome of phlegm and blood stasis. These metabolites could be diagnostic and prognostic biomarkers.展开更多
基金Supported by The Hebei Province TCM Administra on Bureau Founda on for Tackling Key Problems of Science and Technology: 2009058
文摘Objective To observe therapeutic effects of acupuncture on the mouse of hyperlipemia and to explore the mechanisms. Methods One hundred and twenty Kunming mice, male, sanitary degree, were randomly divided into normal group （n=40）, a model group （n=40）, an acupuncture group （n=20） and a medicine group （n=20）. Except the normal group, the mice were fed with high fat forage to prepare mouse hyperlipemia model. On the 15th day of modeling, serum total cholesterol （TC）, triglyceride （TG）, low density lipoprotein-cholesterol （LDL-C）, high density lipoprotein-cholesterol （HDL-C） levels were detected in 20 normal mice and 20 model mice; electroacupuncture was given at bilateral ＂Fēnglóng＂ （丰隆 ST 40）, ＂Qūchí＂ （曲池 LI 11）, ＂Sānyīnjiāo＂ （三阴交 SP 6） in the acupuncture group, once a day, for 10 consecutive days; in the medicine group, the mice were intragastrically administrated with Simvastatin, once daily , for 10 consecutive days. After the end of treatment, serum TC, TG, LDL-C, HDL-C, malondialdehyde （MDA）, nitric oxide （NO） and endothelin （ET） contents and superoxide dismutase （SOD） activities were detected in the groups. Results On the 15th day of modeling, in the model group serum TG, TC, LDL-C contents were significantly higher （all P〈0.05） and HDL-C content was significantly lower （P〈0.01） than those in the normal group. After treatment for 10 days, in the acupuncture group and the medicine group serum TG, TC, LDL-C, MDA and ET contents were significantly lower and serum HDL-C and NO contents and SOD activities were significantly higher than those in the model group （P〈0.05, P〈0.01）, and the improving action in the acupuncture group was better than that in the medicine group （P〈0.05, P〈0.01）. Conclusion Acupuncture can regulate fat metabolism, resist lipid peroxidation and protect vascular endothelial function in the mouse of hyperlipemia.
基金Supported by the National Natural Science Foundation of China for "Combining proteomics and metabolomics to research biological markers group of hyperlipoidemia and arteriosclerosis with the syndrome of phlegm-blood stasis" (No.90709008)National Science and Technology Major Projects for "Major New Drugs Innovation and Development"(2009ZX09502-018)
文摘OBJECTIVE： To explore the plasma metabolite pro- files in patients with the syndrome of phlegm and blood stasis in hyperlipidemia and atherosclerosis （As）, and to search for the metabolic biomarkers of the syndrome. METHODS： The plasma metabolite profiles of 31 patients with the syndrome of phlegm and blood stasis in hyperlipidemia and As, 6 patients with syn- dromes without phlegm and blood stasis, and 10 healthy subjects were analyzed by gas chromatog- raphy-mass spectrometry （GC-MS）. Partial least squares-discriminant analyses （PLS-DA） were used to carry out the pattern-recognition analyses of the data. The plasma metabolic biomarkers of patients were obtained by variable importance plot value （VlP value） and Student＇s t-test. The structures ofbiomarkers were defined by the National Institute of Standards and Technology （NIST） database. RESULTS： PLS-DA score plots of plasma metabo- Iomes did not show overlap between the phlegm-blood stasis syndrome group and syn- dromes without phlegm and blood stasis group, whereas significant differences in the concentra- tions in the plasma of 5 metabolites were found （P〈 0.05）. They were identified as urine, isoleucine, gluc- uronic acid, palmitic acid and glycerol by searching in NIST database. The concentrations of four metab- olites in the plasma of patients with syndrome of phlegm and blood stasis were higher than those with syndromes without phlegm and blood stasis, whereas the glycerol concentration was lower. CONCLUSION： Compared with patients with syn- dromes without phlegm and blood stasis, five me- tabolites showed abnormal levels in patients with the syndrome of phlegm and blood stasis. These metabolites could be diagnostic and prognostic biomarkers.
