Aim To investigate the plant origin and the identity of the red substance on the surface of Hong Dangshen, a unique medicinal material for diarrhea in Hong Kong. Methods The HPLC fingerprints and 5S rRNA gene spacer s...Aim To investigate the plant origin and the identity of the red substance on the surface of Hong Dangshen, a unique medicinal material for diarrhea in Hong Kong. Methods The HPLC fingerprints and 5S rRNA gene spacer sequences of Hong Dangshen were obtained and compared with those of genuine species of Radix Codonopsis. The X-ray diffraction spectrum of the red substance was analyzed and compared with that of Halloysitum fingerprints to the Codonopsis species and the highest similarity to Rubrum. Results Hong Dangshen showed very similar HPLC Codonopsis pilosula var. modesta in terms of 5S rRNA gene spacer sequences. The X-ray diffraction spectrum of the red substance was consistent with that of Halloysitum Rubrum. Conclusion The source plant of Hong Dangshen was suggested to be Codonopsis pilosula var. modesta, one of the genuine original plants of Radix Codonopsis （Dangshen） in the China Pharmacopoeia （2005 edition）. The red substance on the surface of Hong Dangshen was indicated to be Halloysitum Rubrum, a traditional medicinal mineral for chronic diarrhea. Our data suggest that Hong Dangshen is derived from the roots of Codonopsis pilosula var. modesta which has been processed with Halloysitum Rubrum, and the name is suggested to be Radix Codonopsis Praeparata Halloysita Rubra.展开更多
The sequence fragment of PHYA, obtained from transcriptome sequencing,was used as the template, and the full-length c DNA sequence of PHYA gene in tall fescue was amplified using 3＇RACE and 5＇RACE techniques. The c ...The sequence fragment of PHYA, obtained from transcriptome sequencing,was used as the template, and the full-length c DNA sequence of PHYA gene in tall fescue was amplified using 3＇RACE and 5＇RACE techniques. The c DNA sequence of PHYA gene has a complete open reading frame（ORF, 293-6 682 bp）, and it encodes a protein composed of 1 129 amino acids. The N-terminal of Fa PHYA is composed of GAF and Phytochrome domains, and its C-terminal contains two repeated PAS domains, one histidine kinase A domain and one histidine kinase-like ATPase domain. The homology analysis showed that the amino acid sequences of Fa PHYA of tall fescue and PHYAs of gramineous plants have higher homologies（85%）, indicating close genetic relationships. However, the homologies between FaPHYA of tall fescue and PHYAs of monocotyledons are lower, indicating far genetic relationships.展开更多
Objective： To explore the effects of hyperbaric oxygen （HBO） treatment on the neuronal apoptosis at an earlier stage and the expressions of Cytochrome C （Cyt C）, Bcl-2 （B-cell lymphoma-2 family） and Bax （Bcl-2...Objective： To explore the effects of hyperbaric oxygen （HBO） treatment on the neuronal apoptosis at an earlier stage and the expressions of Cytochrome C （Cyt C）, Bcl-2 （B-cell lymphoma-2 family） and Bax （Bcl-2 associated X protein） in rat brain tissues after traumatic brain injury （TBI）. Methods： Forty adult rats were divided into two groups, i.e. ,Group A （the rats with untreated TBI） and Group B （ rats with HBO treatment after TBI）. Sections of brain tissues of these two groups were then detected at 3,6, 12,24,72 hours after TBI by immunohistochemistry and Results： HBO treatment could up-regulate the expression of Bcl-2 within 72 hours, reduce the release of Cyt C from mitochondria, attenuate the formation of dimeric Bax and alleviate the mitochondrial edema within 24 hours after TBI. Conclusions： HBO treatment can alleviate neuronal apoptosis after TBI by reducing the release of Cyt C and the dimers of Bax and up-regulating the expression of Bcl-2.展开更多
基金Hong Kong Jockey Club Charities Trust (ProjectNo. HKJCICM-2-02R).
文摘Aim To investigate the plant origin and the identity of the red substance on the surface of Hong Dangshen, a unique medicinal material for diarrhea in Hong Kong. Methods The HPLC fingerprints and 5S rRNA gene spacer sequences of Hong Dangshen were obtained and compared with those of genuine species of Radix Codonopsis. The X-ray diffraction spectrum of the red substance was analyzed and compared with that of Halloysitum fingerprints to the Codonopsis species and the highest similarity to Rubrum. Results Hong Dangshen showed very similar HPLC Codonopsis pilosula var. modesta in terms of 5S rRNA gene spacer sequences. The X-ray diffraction spectrum of the red substance was consistent with that of Halloysitum Rubrum. Conclusion The source plant of Hong Dangshen was suggested to be Codonopsis pilosula var. modesta, one of the genuine original plants of Radix Codonopsis （Dangshen） in the China Pharmacopoeia （2005 edition）. The red substance on the surface of Hong Dangshen was indicated to be Halloysitum Rubrum, a traditional medicinal mineral for chronic diarrhea. Our data suggest that Hong Dangshen is derived from the roots of Codonopsis pilosula var. modesta which has been processed with Halloysitum Rubrum, and the name is suggested to be Radix Codonopsis Praeparata Halloysita Rubra.
基金Supported by National Natural Science Foundation of China(31360576)~~
文摘The sequence fragment of PHYA, obtained from transcriptome sequencing,was used as the template, and the full-length c DNA sequence of PHYA gene in tall fescue was amplified using 3＇RACE and 5＇RACE techniques. The c DNA sequence of PHYA gene has a complete open reading frame（ORF, 293-6 682 bp）, and it encodes a protein composed of 1 129 amino acids. The N-terminal of Fa PHYA is composed of GAF and Phytochrome domains, and its C-terminal contains two repeated PAS domains, one histidine kinase A domain and one histidine kinase-like ATPase domain. The homology analysis showed that the amino acid sequences of Fa PHYA of tall fescue and PHYAs of gramineous plants have higher homologies（85%）, indicating close genetic relationships. However, the homologies between FaPHYA of tall fescue and PHYAs of monocotyledons are lower, indicating far genetic relationships.
文摘Objective： To explore the effects of hyperbaric oxygen （HBO） treatment on the neuronal apoptosis at an earlier stage and the expressions of Cytochrome C （Cyt C）, Bcl-2 （B-cell lymphoma-2 family） and Bax （Bcl-2 associated X protein） in rat brain tissues after traumatic brain injury （TBI）. Methods： Forty adult rats were divided into two groups, i.e. ,Group A （the rats with untreated TBI） and Group B （ rats with HBO treatment after TBI）. Sections of brain tissues of these two groups were then detected at 3,6, 12,24,72 hours after TBI by immunohistochemistry and Results： HBO treatment could up-regulate the expression of Bcl-2 within 72 hours, reduce the release of Cyt C from mitochondria, attenuate the formation of dimeric Bax and alleviate the mitochondrial edema within 24 hours after TBI. Conclusions： HBO treatment can alleviate neuronal apoptosis after TBI by reducing the release of Cyt C and the dimers of Bax and up-regulating the expression of Bcl-2.
