Aim The several species of the genus Paris called ＂Chonglou＂ are famous traditional Chinese herbal medicines. We established the quantitative analysis method of the steroidal saponins in some species of the genus Pa...Aim The several species of the genus Paris called ＂Chonglou＂ are famous traditional Chinese herbal medicines. We established the quantitative analysis method of the steroidal saponins in some species of the genus Paris and discussed their relations. Methods We detected the contents of 11 steroidal saponins in Paris samples with a Kromasel C18 （ 150 mm× 4.6 mm ID, 5μm） column which was subjected to gradient elution with acetonitrile-water （30：70- 60：40, V/V） at a flow rate of 1 mL· min^-1 by HPLC-ELSD and established chemical cluster tree using SPSS 11 software. Results All the samples could be separated and calibration curves of 11 saponins were prepared. We successfully detected the contents of 11 steroidal saponins in 14 Paris spp. in 30 min. The recovery for the assay of saponins was between 95 % and 97 %. The RSD of precision of 11 saponins and stability of samples were below 3 %. Chemical phylogenetic tree based on saponin contents indicated that 17 samples of Paris spp. clustered separately. Conclusion The established method is accurate and convenient, and suitable for the quantitative analysis of these 11 steroidal saponins in Paris spp.. The chemical phylogenetic tree is in accordance with Takhtajian classical taxonomy.展开更多
为适应临床上已广泛使用的低新鲜气流量吸入全麻,减少麻醉药的浪费和环境污染,降低蒸发器只能专用于一种麻醉药所造成的浪费,我们改造Tec3型三氯乙烯蒸发器,用单片机控制,研制成了多用电控高效能蒸发器。材料与方法一、材料美国Omeda Ex...为适应临床上已广泛使用的低新鲜气流量吸入全麻,减少麻醉药的浪费和环境污染,降低蒸发器只能专用于一种麻醉药所造成的浪费,我们改造Tec3型三氯乙烯蒸发器,用单片机控制,研制成了多用电控高效能蒸发器。材料与方法一、材料美国Omeda Exce1210麻醉机,芬兰Capnomac Ultima Detax多功能气体监测仪;美国雅培生产的安氟醚和异氟醚,日本丸石公司的七氟醚,上海仪表厂的三相步进电机,浙江启东电脑设备厂的EPROM,机电部49所的温度传感器(准确性±0.1℃)及Tec3型三氯乙烯蒸发器。二、三氯乙烯蒸发器的改造将其刻度盘拆除。展开更多
文摘Aim The several species of the genus Paris called ＂Chonglou＂ are famous traditional Chinese herbal medicines. We established the quantitative analysis method of the steroidal saponins in some species of the genus Paris and discussed their relations. Methods We detected the contents of 11 steroidal saponins in Paris samples with a Kromasel C18 （ 150 mm× 4.6 mm ID, 5μm） column which was subjected to gradient elution with acetonitrile-water （30：70- 60：40, V/V） at a flow rate of 1 mL· min^-1 by HPLC-ELSD and established chemical cluster tree using SPSS 11 software. Results All the samples could be separated and calibration curves of 11 saponins were prepared. We successfully detected the contents of 11 steroidal saponins in 14 Paris spp. in 30 min. The recovery for the assay of saponins was between 95 % and 97 %. The RSD of precision of 11 saponins and stability of samples were below 3 %. Chemical phylogenetic tree based on saponin contents indicated that 17 samples of Paris spp. clustered separately. Conclusion The established method is accurate and convenient, and suitable for the quantitative analysis of these 11 steroidal saponins in Paris spp.. The chemical phylogenetic tree is in accordance with Takhtajian classical taxonomy.
文摘为适应临床上已广泛使用的低新鲜气流量吸入全麻,减少麻醉药的浪费和环境污染,降低蒸发器只能专用于一种麻醉药所造成的浪费,我们改造Tec3型三氯乙烯蒸发器,用单片机控制,研制成了多用电控高效能蒸发器。材料与方法一、材料美国Omeda Exce1210麻醉机,芬兰Capnomac Ultima Detax多功能气体监测仪;美国雅培生产的安氟醚和异氟醚,日本丸石公司的七氟醚,上海仪表厂的三相步进电机,浙江启东电脑设备厂的EPROM,机电部49所的温度传感器(准确性±0.1℃)及Tec3型三氯乙烯蒸发器。二、三氯乙烯蒸发器的改造将其刻度盘拆除。
