目的:探讨有氧运动对自发性高血压大鼠(spontaneous hypertension rat,SHR)心肌钙调神经磷酸酶/活化T细胞因子(calcineurin/nuclear factor of activated T cells, CaN/NFAT)信号通路的影响,以及A激酶锚定蛋白150(A-kinaseanchoringprot...目的:探讨有氧运动对自发性高血压大鼠(spontaneous hypertension rat,SHR)心肌钙调神经磷酸酶/活化T细胞因子(calcineurin/nuclear factor of activated T cells, CaN/NFAT)信号通路的影响,以及A激酶锚定蛋白150(A-kinaseanchoringprotein150,AKAP150)在其中的作用。方法:12周龄雄性SHR以及正常血压对照组大鼠(Wistar-Kyotorat,WKY),随机分为正常血压安静组(WKY-SED),正常血压运动组(WKY-EX),高血压安静组(SHR-SED),高血压运动组(SHR-EX)。运动组进行12周中等强度跑台运动。12周后取心脏,HE染色、Langendorff离体心脏灌流、免疫组化、免疫细胞荧光、Western blot等方法进行实验。结果:1)经12周有氧运动,WKY和SHR运动组收缩压均显著低于各自安静组;2)与WKY-SED组相比,SHR-SED组左心室内压上升最大速率(maximal rate of increase in left ventricular pressure,+dp/dt_(max))、左心室内压下降最大速率(maximal rate of decrease in left ventricular pressure,-dp/dt_(max))绝对值显著降低,SHR-EX组与SHR-SED组相比,+dp/dt_(max)、-dp/dt_(max)绝对值显著升高(P<0.01),左心室收缩压力(leftventricularsystolicpressure,LVSP)升高(P<0.01),LVSP-LVDP升高(P<0.01);3)SHR-SED组CaN和AKAP150的荧光强度高于WKY-SED组(P<0.01),CaN表达高于SHR-EX组(P<0.01);SHR-EX组AKAP150的荧光强度高于SHR-SED组(P<0.01);4)SHR-SED组CaN、GATA结合蛋白4(GATAbindprotein,GATA4)及AKAP150的蛋白表达高于WKY-SED组(P<0.01),p-NFATc3/NFATc3的比值显著低于WKYSED组;SHR-EX组CaN、GATA4的表达低于SHR-SED组(P<0.01),p-NFATc3/NFATc3的比值和AKAP150的表达均高于SHR-SED组。结论:有氧运动下调SHR心脏CaN/NFAT信号通路活性、增加调节因子AKAP150的表达,是运动改善其心脏肥大的分子机制之一。展开更多
Coronary blood flow reserve (CFR) was assessed by transesophageal Doppler echocardiography in normal subjects (group A. n=20),hypertensive non-left ventricular hypertrophy (non-LVH)Patients (group B,n=22). hypertensiv...Coronary blood flow reserve (CFR) was assessed by transesophageal Doppler echocardiography in normal subjects (group A. n=20),hypertensive non-left ventricular hypertrophy (non-LVH)Patients (group B,n=22). hypertensive patients with LVH(group C.n=32)and coronary heart disease patients (group D. n=33) with the volume sample placed at the bifurcation of the left main and left main and left descending coronary artery. Coronary blood flow velocity (CBFV)was evaluated at rest.2 minutes after dipyridamole (0. 56mg/kg. i. v.) . and 2 minutes after aminophylline i.v. The ratio of dipyridamole to rest maximal diastolic velocity (D/R PDV) was considered the index of coronary blood flow reserve.It was found that D/R PDV was significantly less in groups C and D compared with that in groups A and B (D PDC,1.84±0. 57. 1. 57±0. 41 versus 2.59±0.70 and 2.22+0.58,respectively),with no difference in D/R PDV between groups C and D.Twenty-four out of 32 patients in group C with D/R PDV were less than 2.0 compared to 29 out of 33 patients in group D (P>0.05).Significant negative correlation was found between D/R PDV. D/R PSV and interseptal thickness. left ventricular mass index in hypertensive patients.These data show that impaired CFR in hypertensive patients with LVH is comparable to that in patients with coronary heart disease.展开更多
文摘目的:探讨有氧运动对自发性高血压大鼠(spontaneous hypertension rat,SHR)心肌钙调神经磷酸酶/活化T细胞因子(calcineurin/nuclear factor of activated T cells, CaN/NFAT)信号通路的影响,以及A激酶锚定蛋白150(A-kinaseanchoringprotein150,AKAP150)在其中的作用。方法:12周龄雄性SHR以及正常血压对照组大鼠(Wistar-Kyotorat,WKY),随机分为正常血压安静组(WKY-SED),正常血压运动组(WKY-EX),高血压安静组(SHR-SED),高血压运动组(SHR-EX)。运动组进行12周中等强度跑台运动。12周后取心脏,HE染色、Langendorff离体心脏灌流、免疫组化、免疫细胞荧光、Western blot等方法进行实验。结果:1)经12周有氧运动,WKY和SHR运动组收缩压均显著低于各自安静组;2)与WKY-SED组相比,SHR-SED组左心室内压上升最大速率(maximal rate of increase in left ventricular pressure,+dp/dt_(max))、左心室内压下降最大速率(maximal rate of decrease in left ventricular pressure,-dp/dt_(max))绝对值显著降低,SHR-EX组与SHR-SED组相比,+dp/dt_(max)、-dp/dt_(max)绝对值显著升高(P<0.01),左心室收缩压力(leftventricularsystolicpressure,LVSP)升高(P<0.01),LVSP-LVDP升高(P<0.01);3)SHR-SED组CaN和AKAP150的荧光强度高于WKY-SED组(P<0.01),CaN表达高于SHR-EX组(P<0.01);SHR-EX组AKAP150的荧光强度高于SHR-SED组(P<0.01);4)SHR-SED组CaN、GATA结合蛋白4(GATAbindprotein,GATA4)及AKAP150的蛋白表达高于WKY-SED组(P<0.01),p-NFATc3/NFATc3的比值显著低于WKYSED组;SHR-EX组CaN、GATA4的表达低于SHR-SED组(P<0.01),p-NFATc3/NFATc3的比值和AKAP150的表达均高于SHR-SED组。结论:有氧运动下调SHR心脏CaN/NFAT信号通路活性、增加调节因子AKAP150的表达,是运动改善其心脏肥大的分子机制之一。
文摘Coronary blood flow reserve (CFR) was assessed by transesophageal Doppler echocardiography in normal subjects (group A. n=20),hypertensive non-left ventricular hypertrophy (non-LVH)Patients (group B,n=22). hypertensive patients with LVH(group C.n=32)and coronary heart disease patients (group D. n=33) with the volume sample placed at the bifurcation of the left main and left main and left descending coronary artery. Coronary blood flow velocity (CBFV)was evaluated at rest.2 minutes after dipyridamole (0. 56mg/kg. i. v.) . and 2 minutes after aminophylline i.v. The ratio of dipyridamole to rest maximal diastolic velocity (D/R PDV) was considered the index of coronary blood flow reserve.It was found that D/R PDV was significantly less in groups C and D compared with that in groups A and B (D PDC,1.84±0. 57. 1. 57±0. 41 versus 2.59±0.70 and 2.22+0.58,respectively),with no difference in D/R PDV between groups C and D.Twenty-four out of 32 patients in group C with D/R PDV were less than 2.0 compared to 29 out of 33 patients in group D (P>0.05).Significant negative correlation was found between D/R PDV. D/R PSV and interseptal thickness. left ventricular mass index in hypertensive patients.These data show that impaired CFR in hypertensive patients with LVH is comparable to that in patients with coronary heart disease.
