目的:观察蒙药德都红花-7味散对非酒精性脂肪肝的治疗作用及其机制,为临床用药提供理论依据.方法:取Wister♂大鼠,随机分为空白组8只、空白给药(德都红花-7味散)、模型组、德都红花-7味散低、高剂量组,各组10只.除空白组和空白给药组,按...目的:观察蒙药德都红花-7味散对非酒精性脂肪肝的治疗作用及其机制,为临床用药提供理论依据.方法:取Wister♂大鼠,随机分为空白组8只、空白给药(德都红花-7味散)、模型组、德都红花-7味散低、高剂量组,各组10只.除空白组和空白给药组,按2 m L/100 g脂肪乳剂灌胃,1次/d,共计给脂肪乳剂灌胃4 wk.空白给药组、德都红花-7味散低、高剂量组分别给予蒙药德都红花-7味散0.6、0.6、3.0 g/(kg·d).各给药组均提前7 d给药,给药体积为1 m L/100 g,1次/d,灌胃给药;其他两组灌胃等体积蒸馏水,共计给药5 wk.观察各组大鼠血脂四项,肝功,肝组织匀浆中总胆固醇(cholesterol,CHO)、甘油三酯(triglyceride,TG)、超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)含量;HE染色观察肝脏细胞脂肪变程度.结果:与空白组比较,模型组大鼠血清TG、CHO、低密度脂蛋白(low density lipoprotein cholesterol,LDL-C)、肝匀浆TG、CHO、MDA、空白给药组MDA升高,血清高密度脂蛋白(high density lipoprotein cholesterol,HDL-C)、肝匀浆SOD明显降低;德都红花-7味散低、高剂量组大鼠血清CHO、LDL-C升高,有显著性差异(P<0.05).与模型组比较,低剂量组血清TG降低,高剂量组血清CHO、LDL-C降低、HDL-C升高,空白给药组肝匀浆TG、CHO、MDA降低,SOD升高;低剂量组肝匀浆TG、CHO、MDA降低,高剂量组肝匀浆TG、MDA降低,SOD升高有显著性差异(P<0.05).给药组肝组织脂肪变程度较模型组明显减轻.结论:蒙药德都红花-7味散治疗高质饮食脂肪肝疗效确切,抗脂质过氧化可能是其治疗机制的一部分.展开更多
The aim of the present study was to investigate the protein-sparing effect of carbohydrate in diets for juvenile turbot(Scophthalmus maximus) reared at five salinities(12,18,24,30,and 36).The fish were fed three isoca...The aim of the present study was to investigate the protein-sparing effect of carbohydrate in diets for juvenile turbot(Scophthalmus maximus) reared at five salinities(12,18,24,30,and 36).The fish were fed three isocaloric and isolipidic diets for 60 days.The results show that specific growth rate(SGR)and feed conversion efficiency(FCE) were higher in fish reared at salinities of 18 and 36,but lower at 12.Fish fed with diet C25P40(25%carbohydrate and 40%protein) had lower SGR and FCE values compared with those fed with the C5P52(5%carbohydrate and 52%protein) and C15P46(15%carbohydrate and 46%protein) diets;however,there was no statistical difference between diet C5P52 and C15P46.SGR and FCE values were unaffected by diet composition in fish reared at salinity 36.Hepatic lipogenic enzyme activities were higher in fish reared at 18 and 36,but lower at 12,while glucokinase(GK) activity was higher in fish reared at 12,and lower at 18 and 36.Dietary starch enhanced GK activity while depressing lipogenic enzyme activity.However,lipogenic enzyme activity increased with increasing dietary starch in fish reared at 36.It is recommended that salinity should be maintained > 12 in the farming of juvenile turbot.In addition,an increase in gelatinized starch from 5%to 15%could spare 6%dietary protein in fish reared at salinities of 18-30,while higher salinity(36) could improve dietary carbohydrate use and enhance the protein-sparing effect,which is linked with the induction of lipogenic capacities.展开更多
文摘目的:观察蒙药德都红花-7味散对非酒精性脂肪肝的治疗作用及其机制,为临床用药提供理论依据.方法:取Wister♂大鼠,随机分为空白组8只、空白给药(德都红花-7味散)、模型组、德都红花-7味散低、高剂量组,各组10只.除空白组和空白给药组,按2 m L/100 g脂肪乳剂灌胃,1次/d,共计给脂肪乳剂灌胃4 wk.空白给药组、德都红花-7味散低、高剂量组分别给予蒙药德都红花-7味散0.6、0.6、3.0 g/(kg·d).各给药组均提前7 d给药,给药体积为1 m L/100 g,1次/d,灌胃给药;其他两组灌胃等体积蒸馏水,共计给药5 wk.观察各组大鼠血脂四项,肝功,肝组织匀浆中总胆固醇(cholesterol,CHO)、甘油三酯(triglyceride,TG)、超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)含量;HE染色观察肝脏细胞脂肪变程度.结果:与空白组比较,模型组大鼠血清TG、CHO、低密度脂蛋白(low density lipoprotein cholesterol,LDL-C)、肝匀浆TG、CHO、MDA、空白给药组MDA升高,血清高密度脂蛋白(high density lipoprotein cholesterol,HDL-C)、肝匀浆SOD明显降低;德都红花-7味散低、高剂量组大鼠血清CHO、LDL-C升高,有显著性差异(P<0.05).与模型组比较,低剂量组血清TG降低,高剂量组血清CHO、LDL-C降低、HDL-C升高,空白给药组肝匀浆TG、CHO、MDA降低,SOD升高;低剂量组肝匀浆TG、CHO、MDA降低,高剂量组肝匀浆TG、MDA降低,SOD升高有显著性差异(P<0.05).给药组肝组织脂肪变程度较模型组明显减轻.结论:蒙药德都红花-7味散治疗高质饮食脂肪肝疗效确切,抗脂质过氧化可能是其治疗机制的一部分.
基金Supported by the Special Fund of Chinese Ministry of Agriculture for Modern Agriculture Industry System Construction(No.CARS-50)
文摘The aim of the present study was to investigate the protein-sparing effect of carbohydrate in diets for juvenile turbot(Scophthalmus maximus) reared at five salinities(12,18,24,30,and 36).The fish were fed three isocaloric and isolipidic diets for 60 days.The results show that specific growth rate(SGR)and feed conversion efficiency(FCE) were higher in fish reared at salinities of 18 and 36,but lower at 12.Fish fed with diet C25P40(25%carbohydrate and 40%protein) had lower SGR and FCE values compared with those fed with the C5P52(5%carbohydrate and 52%protein) and C15P46(15%carbohydrate and 46%protein) diets;however,there was no statistical difference between diet C5P52 and C15P46.SGR and FCE values were unaffected by diet composition in fish reared at salinity 36.Hepatic lipogenic enzyme activities were higher in fish reared at 18 and 36,but lower at 12,while glucokinase(GK) activity was higher in fish reared at 12,and lower at 18 and 36.Dietary starch enhanced GK activity while depressing lipogenic enzyme activity.However,lipogenic enzyme activity increased with increasing dietary starch in fish reared at 36.It is recommended that salinity should be maintained > 12 in the farming of juvenile turbot.In addition,an increase in gelatinized starch from 5%to 15%could spare 6%dietary protein in fish reared at salinities of 18-30,while higher salinity(36) could improve dietary carbohydrate use and enhance the protein-sparing effect,which is linked with the induction of lipogenic capacities.
